Cooperation of the molecular chaperone Ydj1 with specific Hsp70 homologs to suppress protein aggregation

AbstractYdj1p, a cytosolic DnaJ homolog from Saccharomyces cerevisiae, is demonstrated to function as a molecular chaperone. Purified Ydj1p formed complexes with non-native polypeptides and suppressed protein aggregation. Ydj1p cooperated with Ssa Hsp70 proteins in the prevention of protein aggregation. but not with the Ssb Hsp70 proteins. Cooperation between these different molecular chaperones was only observed in the presence of hydrolyzable ATP and correlated with the ability of Ydj1p to stimulate the ATPase activity of the Hsp70 homolog with which it was paired. The regulatory and chaperone activities of a eukaryotic DnaJ homolog thus act together to assist Hsp70 in modulating the conformation of proteins

Peralihan Hak Milik Atas Tanah Akibat Hibah Menurut Undang-undang Nomor 5 Tahun 1960 Tentang Pokok-pokok Agraria

Penelitian ini dilakukan dengan tujuan untuk mengetahui bagaimana asas dan tujuan pendaftaran tanah dan bagaimana peralihan hak milik atas tanah karena hibah menurut Undang-Undang Nomor 5 Tahun 1960 tentang Pokok-pokok Agraria. Dengan menggunakan metode penelitian yuridis normatif disimpulkan: 1. Pendaftaran tanah dikenal asas specialiteit dan Asas Openbaarheid (Asas Publisitas) serta menurut PP Nomor 4 tahun 1997 dengan asas sederhana,aman, terjangkau, mutakhir dan asas terbuka dan tujuan pendaftaran tanah meliputi, kepastian hukum dan perlindungan hukum kepada pemegang hak atas suatu bidang tanah dan manfaat bagi pemegang hak dengan diselenggarakan pendaftaran tanah adalah memberikan rasa aman, mengetahui dengan jelas data fisik dan data yuridis, memudahkan dalam pelaksanaan peralihan hak, harga tanah menjadi lebih tinggi, dapat dijadikan jaminan utang dengan dibebani Hak Tanggungan, penetapan PBB tidak mudah keliru serta manfaat bagi Pemerintah adalah terwujudnya tertib administrasi, memperlancar kegiatan Pemerintahan yang berkaitan dengan tanah dalam pembangunan dan mengurangi sengketa di bidang pertanahan. 2. Peralihan hak milik atas tanah karena hibah dasar hukumnya terdapat dalam Pasal 20 ayat 1, Pasal 26 ayat 1 dan ayat 2, Pasal 20 ayat 1 dan 2, Pasal 28 ayat 2 dan 3, Pasal 35 ayat 3 , Pasal 43 Undang-Undang Nomor 5 Tahun 1960, Undang-Undang Nomor 16 Tahun 1985 tentang Rumah Susun Pasal 10 ayat 1 dan Peraturan Pemerintah Nomor 24 Tahun 1997 tentang Pendaftaran Tanah yang terdapat dalam Pasal 37 ayat 1 dan ayat 4, Pasal 41 dan 42 , Peraturan Pemerintah Nomor 40 Tahun 1996 yang terdapat dalam Pasal 34 ayat 2 dan ayat 7, Peraturan Pemerintah Nomor 40 Tahun 1996 yang terdapat dalam Pasal 16 ayat 2 dan terdapat dalam Pasal 34 dan Pasal 54

A Racial Impact Analysis of HB 936: Accessibility of Electronic Textbooks

The economic and social consequences of the lack of access to technology for students in the Commonwealth of Virginia are real and significant. This report provides a legislative racial impact analysis of House Bill (HB) 936, a proposed bill in the Virginia General Assembly, prohibiting school boards from making electronic textbooks available for students, unless the school board adopts a plan to ensure that e-textbooks are available on or before July 1, 2017. The bill focuses solely on developing a plan for installing prior to implementing electronic textbooks in K-12 classrooms. Electronic textbooks are important as they offer updated content, ease of accessibility, multimedia features to enhance the learning experience, and the ability for educators to customize learning. Delegating this decision to each local school board in Virginia raises important potential racial implications, including the digital divide. Previous research suggests a correlation between the number of students receiving free and reduced lunch and the lack of availability of electronic textbooks. Districts with high rates of students on free and reduced lunch have a high population of minority students. This analysis provides maps that capture the trends on the probability of providing electronic textbooks for high minority areas across the state. The primary recommendation is to advance policy approaches that make electronic textbooks available to all K‐12 students in the Commonwealth of Virginia

Roundtable Convening on Women and Youth SMEs in Agribusiness

The International Institute of Tropical Agriculture collaborated with The Rallying Cry to understand the challenges faced by women- and youth-led agribusinesses in Zambia. As such, two workshops were previously held in September and December 2022 with the target group to obtain an understanding of their challenges and identify possible solutions. These challenges were categorised into themes: (a) Environmental constraints; (b) Network; (c) Climate change response; (d) Capacity Building; and (e) Social and cultural norms. The women and youth in attendance generated solutions and shared wish list items in both workshops. This output framed and provided the basis for the Roundtable convening held on 2 March 2023. A range of stakeholders from across the private and public sector ecosystem were invited to participate in a full day engagement of co-designing solutions for identified challenges, identifying relevant roles for stakeholder action and developing a blueprint for possible next steps. Key highlights from the event confirmed the challenges and potential solutions previously identified by the women and youth. Stakeholders from local capital providers, entrepreneur support organisations and relevant government ministries provided input into and expanded possible solutions and roles for applicable ecosystem players. Lastly, the events of the day showed that many of the identified solutions are interdependent, and that tangible change will require multi-sector interventions. To implement the actions and recommendations from the Roundtable, a ‘task force’ could be assembled to develop a detailed yearlong workplan to support selected and agreed solutions. Updates can then be shared at subsequent Roundtables; this could become an annual event that draws diverse stakeholders to a single forum. We also see the longer term need to develop an ecosystem initiative for relevant stakeholders that connects siloed activities and objectives and includes the development of a more intentional and supported network for women and youth agripreneurs

Genetic programming-based regression for temporal data

Various machine learning techniques exist to perform regression on temporal data with concept drift occurring. However, there are numerous nonstationary environments where these techniques may fail to either track or detect the changes. This study develops a genetic programming-based predictive model for temporal data with a numerical target that tracks changes in a dataset due to concept drift. When an environmental change is evident, the proposed algorithm reacts to the change by clustering the data and then inducing nonlinear models that describe generated clusters. Nonlinear models become terminal nodes of genetic programming model trees. Experiments were carried out using seven nonstationary datasets and the obtained results suggest that the proposed model yields high adaptation rates and accuracy to several types of concept drifts. Future work will consider strengthening the adaptation to concept drift and the fast implementation of genetic programming on GPUs to provide fast learning for high-speed temporal data.http://link.springer.com/journal/107102022-05-09hj2021Computer Scienc

Redesigning the Warren Animal Shelter

The new shelter should incorporate improved natural lighting and ventilation, adequate space for laundry facilities and more space for the animals\u27 living and bathing needs. The design should consider how to prevent dogs from barking and disturbing one another while in the kennels. Increased storage is necessary for the animal shelter to contain the food and living amenities for the incoming animals

Behavior of metakaolin-based geopolymers incorporating sewage sludge ash (SSA)

[EN] In recent years, geopolymers have become a widely researched binding material. There are technological and environmental advantages tousing this type of binder instead of Portland cement. In this study, binary systems of geopolymers were produced by using mixtures of metakaolin (MK) ,a well-known aluminosilicate raw material, and a residue from sewage sludge incineration: sewage sludge ash (SSA). This ash was used to partially replace the metakaolin in proportions of 0 20%. The mixtures were activated with alkaline solutions and they were cured by using two different conditions: at room temperature (25 °C) and in a thermal bath (65 °C). The samples were assessed by X-ray diffraction, scanning electron microscopy (pastes) and compressive strength (mortars). The results from these studies showed zeolite formation (faujasite) in geopolymers cured in the thermal bath, which caused a decrease in the compressive strength of the alkali-activated mortars.Replacement of MK with SSA caused a lower reduction in the compressive strength of mortars cured at 65 °C. However, at room temperature, similar mechanical strength was observed for the MK and MK-SSA systems. These results demonstrated that SSA is a suitable mineral precursor for partial replacement of MK in geopolymer production.The authors acknowledge Santander Universidades for the grant to Lucia Reig (program: Becas lberoamerica Jovenes Profesores Investigadores Espana 2014), CAPES (CAPES/DGU no 266/12), CNPq (no. 14/2013 processo 478057/2013-0) Scanning electron microscopy service of FEIS/UNESP and CNPq (processo 309015/2015-4).Istuque, D.; Reig Cerdá, L.; Moraes, J.; Akasaki, JL.; Borrachero Rosado, MV.; Soriano Martínez, L.; Paya Bernabeu, JJ.... (2016). Behavior of metakaolin-based geopolymers incorporating sewage sludge ash (SSA). Materials Letters. 180:192-195. https://doi.org/10.1016/j.matlet.2016.05.137S19219518

Trick film: Neil Brand’s radio dramas and the silent film experience

At first glance, silent film and audio drama may appear antithetical modes of expression. Nevertheless, an interesting tradition of silent film-to-radio adapta-tions has emerged on BBC Radio Drama. Beyond this link between silent film and radio drama, other radio dramas have highlighted that Neil Brand, a successful silent film accompanist, radio dramatist and composer, links the silent film expe-rience and audio drama in two of his plays, Joanna and Waves Breaking on a Shore. Using theories of sound and narrative in film and radio, as well as discuss-ing the way radio in particular can stimulate the generation of imagery, this arti-cle examines layered points of view/audition as ways of linking the silent film experience and the use of sound within radio drama

Le FORUM, Vol. 38 No. 3

https://digitalcommons.library.umaine.edu/francoamericain_forum/1042/thumbnail.jp

Identification and selective expansion of functionally superior T cells expressing chimeric antigen receptors

Background: T cells expressing chimeric antigen receptors (CARs) have shown exciting promise in cancer therapy, particularly in the treatment of B-cell malignancies. However, optimization of CAR-T cell production remains a trial-and-error exercise due to a lack of phenotypic benchmarks that are clearly predictive of anti-tumor functionality. A close examination of the dynamic changes experienced by CAR-T cells upon stimulation can improve understanding of CAR–T-cell biology and identify potential points for optimization in the production of highly functional T cells. Methods: Primary human T cells expressing a second-generation, anti-CD19 CAR were systematically examined for changes in phenotypic and functional responses to antigen exposure over time. Multi-color flow cytometry was performed to quantify dynamic changes in CAR-T cell viability, proliferation, as well as expression of various activation and exhaustion markers in response to varied antigen stimulation conditions. Results: Stimulated CAR-T cells consistently bifurcate into two distinct subpopulations, only one of which (CARhi/CD25+) exhibit anti-tumor functions. The use of central memory T cells as the starting population and the resilience—but not antigen density—of antigen-presenting cells used to expand CAR-T cells were identified as critical parameters that augment the production of functionally superior T cells. We further demonstrate that the CARhi/CD25+ subpopulation upregulates PD-1 but is resistant to PD-L1-induced dysfunction. Conclusions: CAR-T cells expanded ex vivo for adoptive T-cell therapy undergo dynamic phenotypic changes during the expansion process and result in two distinct populations with dramatically different functional capacities. Significant and sustained CD25 and CAR expression upregulation is predictive of robust anti-tumor functionality in antigen-stimulated T cells, despite their correlation with persistent PD-1 upregulation. The functionally superior subpopulation can be selectively augmented by careful calibration of antigen stimulation and the enrichment of central memory T-cell type. Electronic supplementary material The online version of this article (doi:10.1186/s12967-015-0519-8) contains supplementary material, which is available to authorized users