Conservation systematics of the shield-backed trapdoor spiders of the nigrum-group (Mygalomorphae, idiopidae, idiosoma): Integrative taxonomy reveals a diverse and threatened fauna from south-western Australia [dataset]

Supplementary material from: Rix MG, Huey JA, Cooper SJB, Austin AD, Harvey MS (2018) Conservation systematics of the shield-backed trapdoor spiders of the nigrum-group (Mygalomorphae, Idiopidae, Idiosoma): integrative taxonomy reveals a diverse and threatened fauna from south-western Australia. ZooKeys 756: 1-121. https://doi.org/10.3897/zookeys.756.24397 Supplementary material 1: Atlas of morphology : Explanation note: Atlas of morphology for shield-backed trapdoor spiders of the Idiosoma nigrum-group, illustrating a representative selection of male specimens for each species, in five standard views. Supplementary material 2: GenBank data : Explanation note: Spreadsheet of specimens sequenced for the molecular analyses, with associated collection data and GenBank accession numbers. Supplementary material 3: COI dataset : Explanation note: Nexus file of the aligned COI dataset for 82 taxa. Supplementary material 4: FULL dataset : Explanation note: Nexus file of the aligned FULL dataset for 82 taxa. Supplementary material 5: COI p-distances : Explanation note: Spreadsheet with matrix of COI p-distances for 61 sequenced specimens of Idiosoma in the nigrum-group, along with a summary of mean inter- and intra-specific p-distances

Across the Indian Ocean: a remarkable example of trans-oceanic dispersal in an austral mygalomorph spider [dataset]

The Migidae are a family of austral trapdoor spiders known to show a highly restricted and disjunct distribution pattern. Here, we aim to investigate the phylogeny and historical biogeography of the group, which was previously thought to be vicariant in origin, and examine the biogeographic origins of the genus Moggridgea using a dated multi-gene phylogeny. Moggridgea specimens were sampled from southern Australia and Africa, and Bertmainus was sampled from Western Australia. Sanger sequencing methods were used to generate a robust six marker molecular dataset consisting of the nuclear genes 18S rRNA, 28S rRNA, ITS rRNA, XPNPEP3 and H3 and the mitochondrial gene COI. Bayesian and Maximum Likelihood methods were used to analyse the dataset, and the key dispersal nodes were dated using BEAST. Based on our data, we demonstrate that Moggridgea rainbowi from Kangaroo Island, Australia is a valid member of the otherwise African genus Moggridgea. Molecular clock dating analyses show that the inter-specific divergence of M. rainbowi from African congeners is between 2.27–16.02 million years ago (Mya). This divergence date significantly post-dates the separation of Africa from Gondwana (95 Mya) and therefore does not support a vicariant origin for Australian Moggridgea. It also pre-dates human colonisation of Kangaroo Island, a result which is further supported by the intra-specific divergence date of 1.10–6.39 Mya between separate populations on Kangaroo Island. These analyses provide strong support for the hypothesis that Moggridgea colonised Australia via long-distance trans-Indian Ocean dispersal, representing the first such documented case in a mygalomorph spider

Three new species of Dolichogenidea Viereck (Hymenoptera, Braconidae, Microgastrinae) from Australia with exceptionally long ovipositors

The subfamily Microgastrinae contains an extraordinarily rich diversity of parasitoid wasps which parasitise larval lepidopterans. The Australian fauna has generally been poorly studied, particularly for the very speciose genera. One such genus is Dolichogenidea Vierek, which in Australia is known from only six described species. Here we describe three new species of Dolichogenidea from Australia, which are distinguished by possessing extremely long ovipositors compared with the typical form for the genus. These are D. finchi Fagan-Jeffries & Austin, sp. n., D. mediocaudata Fagan-Jeffries & Austin, sp. n., and D. xenomorph Fagan-Jeffries & Austin, sp. n. In describing these new species we also discuss relationships within the genus, and the diversity and biology of the Australian fauna

Reasons to Be Skeptical about Sentience and Pain in Fishes and Aquatic Invertebrates

The welfare of fishes and aquatic invertebrates is important, and several jurisdictions have included these taxa under welfare regulation in recent years. Regulation of welfare requires use of scientifically validated welfare criteria. This is why applying Mertonian skepticism toward claims for sentience and pain in fishes and aquatic invertebrates is scientifically sound and prudent, particularly when those claims are used to justify legislation regulating the welfare of these taxa. Enacting welfare legislation for these taxa without strong scientific evidence is a societal and political choice that risks creating scientific and interpretational problems as well as major policy challenges, including the potential to generate significant unintended consequences. In contrast, a more rigorous science-based approach to the welfare of aquatic organisms that is based on verified, validated and measurable endpoints is more likely to result in “win-win” scenarios that minimize the risk of unintended negative impacts for all stakeholders, including fish and aquatic invertebrates. The authors identify as supporters of animal welfare, and emphasize that this issue is not about choosing between welfare and no welfare for fish and aquatic invertebrates, but rather to ensure that important decisions about their welfare are based on scientifically robust evidence. These ten reasons are delivered in the spirit of organized skepticism to orient legislators, decision makers and the scientific community, and alert them to the need to maintain a high scientific evidential bar for any operational welfare indicators used for aquatic animals, particularly those mandated by legislation. Moving forward, maintaining the highest scientific standards is vitally important, in order to protect not only aquatic animal welfare, but also global food security and the welfare of humans

Genetic Sharing with Cardiovascular Disease Risk Factors and Diabetes Reveals Novel Bone Mineral Density Loci.

Bone Mineral Density (BMD) is a highly heritable trait, but genome-wide association studies have identified few genetic risk factors. Epidemiological studies suggest associations between BMD and several traits and diseases, but the nature of the suggestive comorbidity is still unknown. We used a novel genetic pleiotropy-informed conditional False Discovery Rate (FDR) method to identify single nucleotide polymorphisms (SNPs) associated with BMD by leveraging cardiovascular disease (CVD) associated disorders and metabolic traits. By conditioning on SNPs associated with the CVD-related phenotypes, type 1 diabetes, type 2 diabetes, systolic blood pressure, diastolic blood pressure, high density lipoprotein, low density lipoprotein, triglycerides and waist hip ratio, we identified 65 novel independent BMD loci (26 with femoral neck BMD and 47 with lumbar spine BMD) at conditional FDR < 0.01. Many of the loci were confirmed in genetic expression studies. Genes validated at the mRNA levels were characteristic for the osteoblast/osteocyte lineage, Wnt signaling pathway and bone metabolism. The results provide new insight into genetic mechanisms of variability in BMD, and a better understanding of the genetic underpinnings of clinical comorbidity

Review of Australian Scirtes Illiger, Ora Clark and Exochomoscirtes Pic (Coleoptera: Scirtidae) including descriptions of new species, new groups and a multi-gene molecular phylogeny of Australian and non-Australian species

Watts, Chris H.S., Cooper, Steven J.B., Saint, Kathleen M. (2017): Review of Australian Scirtes Illiger, Ora Clark and Exochomoscirtes Pic (Coleoptera: Scirtidae) including descriptions of new species, new groups and a multi-gene molecular phylogeny of Australian and non-Australian species. Zootaxa 4347 (3): 511-532, DOI: https://doi.org/10.11646/zootaxa.4347.3.

Moggridgea_COI_nex

A text file containing the nexus input file of COI sequences from 103 Moggridgea spiders, including 95 Moggridgea tingle from south-western Australia (T numbers), 5 Moggridgea species from Africa and 3 Moggridgea australis from Kangaroo Island, South Australia. This nexus file was used to produce COI phylogenetic trees presented in the Cooper et al. (2011) Molecular Ecology paper

Moggridgea_COI_reduced_nex

A text file containing the nexus input file of COI sequences from 47 Moggridgea spiders, including 46 Moggridgea tingle from south-western Australia (T numbers), and one Moggridgea australis from Kangaroo Island, South Australia. This nexus file contains a reduced COI dataset after removal of identical haplotype sequences and was used to produce COI phylogenetic trees presented in the Cooper et al. (2011) Molecular Ecology paper

Moggridgea_ITS_nex

This file is a text file containing 53 ITS rRNA sequences from Moggridgea tingle (T numbers), 3 from Moggridgea australis (Mog1-3) and 3 African Moggridgea (MY numbers). It was used to produce phylogenetic trees given in the Cooper et al. (2011) Molecular Ecology paper