1,133 research outputs found

    Orange II removal by a horseradish peroxidase immobilized onto chemically modified diatomites is a combination of adsorption and oxidation processes

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    In this work, a horseradish peroxidase (HRP) was immobilized onto diatomites by covalent bonding. Results indicated that the enzyme loading increased when diatomites were modified with (3-Aminopropyl) triethoxysilane (APTES) and glutaraldehyde. The immobilization was confirmed by SEM/EDX, XRD, DRIFT, and TGA analysis. Higher HRP concentrations of the immobilization solution and immobilization time had also a positive effect on the enzyme loading. Orange II (OII) adsorption onto diatomites and oxidative catalytic activity was evaluated. Results demonstrated that diatomites had a low OII adsorption capacity. However, under the presence of hydrogen peroxide, the dye removal was highly increased due to the catalytic activity of the immobilized HRP. A mathematical model that adequately describes the simultaneous adsorption and enzymatic oxidation of OII in batch tests was developed. Finally, immobilized diatomites were tested in the decolourization reaction of Orange II in a fixed-bed column reactor. Column results demonstrated that the immobilized HRP remained active for at least 12 h during three sequential OII removal tests.Fil: Morales Urrea, Diego Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; ArgentinaFil: Haure, Patricia Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Departamento de Ingeniería Química; ArgentinaFil: Contreras, Edgardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Ciencia y Tecnología de Materiales. Universidad Nacional de Mar del Plata. Facultad de Ingeniería. Instituto de Investigaciones en Ciencia y Tecnología de Materiales; Argentin

    Anomaly detection in laser-guided vehicles' batteries: a case study

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    Detecting anomalous data within time series is a very relevant task in pattern recognition and machine learning, with many possible applications that range from disease prevention in medicine, e.g., detecting early alterations of the health status before it can clearly be defined as "illness" up to monitoring industrial plants. Regarding this latter application, detecting anomalies in an industrial plant's status firstly prevents serious damages that would require a long interruption of the production process. Secondly, it permits optimal scheduling of maintenance interventions by limiting them to urgent situations. At the same time, they typically follow a fixed prudential schedule according to which components are substituted well before the end of their expected lifetime. This paper describes a case study regarding the monitoring of the status of Laser-guided Vehicles (LGVs) batteries, on which we worked as our contribution to project SUPER (Supercomputing Unified Platform, Emilia Romagna) aimed at establishing and demonstrating a regional High-Performance Computing platform that is going to represent the main Italian supercomputing environment for both computing power and data volume.Comment: This paper contains a report on the research work carried out as a collaboration between the Department of Engineering and Architecture of the University of Parma and Elettric80 spa within project SUPER (Supercomputing Unified Platform Emilia Romagna

    Trabajo Remoto, Habilidades Sociales y el Rendimiento Escolar de los Estudiantes del Nivel Primario en la Institución Educativa 449 Eduardo Pérez Gamboa de la Ciudad de Tacna

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    El objetivo de este trabajo de investigación fueron los principales estudio en determinar el nivel de relación que existe entre el trabajo a distancia y las habilidades sociales y el rendimiento académico de los estudiantes de primaria de la Institución Educativa 449. Eduardo Pérez Gamboa, residente en Tacna Actualmente estamos viviendo una pandemia mundial causada por el virus COVID 19, que está provocando el aislamiento de la población a nivel mundial y haciendo que la educación primaria se convierta en educación secundaria. El enfoque de este estudio fue cuantitativo, ya que la recolección de datos se utilizará para demostrar las hipótesis basadas en cálculos numéricos y análisis estadísticos. La investigación es de tipo básico y multivariado, sin diseño experimental.The objective of this research work were the main studies to determine the level of relationship that exists between remote work and social skills and academic performance of primary students of Educational Institution 449. Eduardo Pérez Gamboa, resident in Tacna We are currently experiencing a global pandemic caused by the virus COVID 19, which is causing the isolation of the population worldwide and making that primary education becomes secondary education. The focus of this study was quantitative, since the data collection will be used to demonstrate the hypotheses based on numerical calculations and statistical analysis. The research is basic and multivariate, without experimental design.ChosicaEscuela de PosGrad

    Motivación y productividad laboral de profesionales de salud del policlínico de Essalud en Lima, 2022

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    La investigación se proyectó en determinar si existe relación entre la motivación y la Productividad Laboral de los profesionales de salud del Policlínico de Essalud en Lima, 2022. La metodología del estudio presenta las siguientes características, es tipo básico, enfoque cuantitativo, de diseño no experimental de corte transversal; y correlacional, ya que analiza el nivel de vinculación las dos variables en estudio. Para ambas variables se aplicó el instrumento del cuestionario en la escala de Likert, donde en la variable motivación se tuvo 21 preguntas, mientras que para la segunda variable Productividad Laboral se tuvo 20 preguntas. La validez del instrumento fue dada por tres profesionales expertos y su confiabilidad fue comprobado por medio del Alpha de Cronbach, en tal sentido se pudo garantizar su fiabilidad. La población total que participo fue de 100 colaboradores del Policlínico de Essalud en Lima, 2022, siendo la misma cantidad para la muestra. El estudio explica que la significancia alcanzada con el estadígrafo rho de Spearman es de 0.001 menor que el 0.05, por lo que se acepta la hipótesis de investigación, además se aprecia una correlación positiva baja (0.326) por lo que se concluye que, si existe relación significativa entre la motivación y la productividad laboral de los profesionales de salud del policlínico de Essalud en Lima, 2022

    Occurrence of Proteus mirabilis associated with two species of venezuelan oysters

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    The fecal contamination of raw seafood by indicators and opportunistic pathogenic microorganisms represents a public health concern. The objective of this study was to investigate the presence of enteric bacteria colonizing oysters collected from a Venezuelan touristic area. Oyster samples were collected at the northwestern coast of Venezuela and local salinity, pH, temperature, and dissolved oxygen of seawater were recorded. Total and fecal coliforms were measured for the assessment of the microbiological quality of water and oysters, using the Multiple Tube Fermentation technique. Analyses were made using cultures and 16S rRNA gene sequencing. Diverse enrichment and selective culture methods were used to isolate enteric bacteria. We obtained pure cultures of Gram-negative straight rods with fimbriae from Isognomon alatus and Crassostrea rhizophorae. Our results show that P. mirabilis was predominant under our culture conditions. We confirmed the identity of the cultures by biochemical tests, 16S rRNA gene sequencing, and data analysis. Other enterobacteria such as Escherichia coli, Morganella morganii and Klebsiella pneumoniae were also isolated from seawater and oysters. The presence of pathogenic bacteria in oysters could have serious epidemiological implications and a potential human health risk associated with consumption of raw seafood.A contaminação fecal de frutos do mar crus por microrganismos oportunistas patogênicos representa problema de saúde pública. O objetivo deste estudo é investigar a presença de bactérias entéricas que colonizam ostras coletadas em área turística da Venezuela. Amostras de ostras foram coletadas na costa noroeste da Venezuela e foram registrados a salinidade local, pH, temperatura e o oxigênio dissolvido na água do mar. O total de coliformes fecais foi medido para a avaliação da qualidade microbiológica da água e das ostras, usando a técnica de fermentação em tubos múltiplos. Análises foram feitas usando culturas e seqüência do gene 16S rRNA. Enriquecimento diversificado e métodos de cultura seletivos foram usados para isolar a bactéria entérica. Obtivemos culturas puras de bastões retos Gram negativos com fímbrias de Isognomon alatus e Crassostrea rhizophorae. Nossos resultados mostram que P. mirabilis foi predominante nas nossas condições de cultura. Confirmamos a identidade das culturas por testes bioquímicos, seqüência do gene 16rRNA e a análise de dados. Outras enterobactérias como Escherichia coli, Morganella morganii e Klebsiella pneumoniae foram também isoladas da água do mar e ostras. A presença de bactérias patogênicas em ostras podem ter implicações epidemiológicas e potencial risco para a saúde humana quando do consumo de frutos do mar crus

    Phylogeographic evidence of cognate recognition site patterns and transformation efficiency differences in H. pylori: theory of strain dominance

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    BACKGROUND: Helicobacter pylori has diverged in parallel to its human host, leading to distinct phylogeographic populations. Recent evidence suggests that in the current human mixing in Latin America, European H. pylori (hpEurope) are increasingly dominant at the expense of Amerindian haplotypes (hspAmerind). This phenomenon might occur via DNA recombination, modulated by restriction-modification systems (RMS), in which differences in cognate recognition sites (CRS) and in active methylases will determine direction and frequency of gene flow. We hypothesized that genomes from hspAmerind strains that evolved from a small founder population have lost CRS for RMS and active methylases, promoting hpEurope’s DNA invasion. We determined the observed and expected frequencies of CRS for RMS in DNA from 7 H. pylori whole genomes and 110 multilocus sequences. We also measured the number of active methylases by resistance to in vitro digestion by 16 restriction enzymes of genomic DNA from 9 hpEurope and 9 hspAmerind strains, and determined the direction of DNA uptake in co-culture experiments of hspAmerind and hpEurope strains. RESULTS: Most of the CRS were underrepresented with consistency between whole genomes and multilocus sequences. Although neither the frequency of CRS nor the number of active methylases differ among the bacterial populations (average 8.6 ± 2.6), hspAmerind strains had a restriction profile distinct from that in hpEurope strains, with 15 recognition sites accounting for the differences. Amerindians strains also exhibited higher transformation rates than European strains, and were more susceptible to be subverted by larger DNA hpEurope-fragments than vice versa. CONCLUSIONS: The geographical variation in the pattern of CRS provides evidence for ancestral differences in RMS representation and function, and the transformation findings support the hypothesis of Europeanization of the Amerindian strains in Latin America via DNA recombination

    Growth of Stone pine (Pinus pinea L.) European provenances in central Chile

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    Pinus pinea is characterized by phenotypic plasticity, tolerance to harsh soils and climates, but low differentiation in growth parameters and low genetic variability. Growth and cone production of six European stone pine provenances (two from Italy, three from Spain and one from Slovenia) were analyzed in a field trial experiment established in central Chile. The study evaluated height, diameter at breast height (DBH) and crown diameter growth of 147 nineteen-year-old trees per provenance, as well as fruiting variables (i.e., number of cones per tree and cone weight). Survival over the first 7 years was also evaluated. Provenances significantly differed in cone number per tree, cone weight, height and DBH growth, and crown diameter growth. Provenances were grouped according to growth and production variables: one group included the Italian and Slovenian provenances, the second group Andalucía and Sierra Morena (Spain), and the third included Meseta Castellana (Spain). Individual cone production was positively correlated with cone weight and other growth variables. Meseta Castellana provenance showed the highest growth and productivity. Our results provide useful information for the selection of P. pinea provenances to be used in new plantations in central Chile.Fil: Loewe Muñoz, Verónica. Chilean Forest Institute; ChileFil: Balzarini, Monica Graciela. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Departamento de Desarrollo Rural. Area de Estadística y Biometría; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Delard Rodríguez, Claudia. Chilean Forest Institute; ChileFil: Álvarez Contreras, Andrea. Chilean Forest Institute; ChileFil: Navarro Cerrillo, Rafael María. Universidad de Córdoba; Españ

    Sistema de información para el hospedaje Mar Azul

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    Este trabajo se basa en una necesidad de la empresa Mar Azul la cual ofrece un servicio de hospedaje en Villavicencio, actualmente está muy bien ubicada y les surgió la necesidad de posicionarse en la red, ya que en la actualidad la tecnología ha avanzado mucho y son muchas las personas que aceden al internet diariamente de esta manera se opta por aprovechar los recursos tecnológicos dando a conocer los servicios que ofrece el hospedaje. En este proyecto se realizó un análisis detallado de los principales requerimientos de la empresa, se elaboró el proyecto utilizando la metodología xp ya que promueve el trabajo en equipo, se basa en una retroalimentación continua entre el cliente y el equipo de desarrollo .de esta manera se mejoró la productividad del proyecto

    Removal of Arsenic from water using synthetic Fe7S8 nanoparticles

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    In the present study, pyrrhotite was used to remove arsenite and arsenate from aqueous solutions. The Fe7S8 was synthesized using a solvothermal synthetic method and it was characterized using XRD and SEM micrographs. Furthermore, the particle size for the nanomaterial Fe7S8 was determined to be 29.86 ± 0.87 nm using Scherer’s equation. During the pH profile studies, the optimum pH for the binding of As (III) and As (V) was determined to be pH 4. Batch isotherm studies were performed to determine the binding capacity of As(III) and As(V), which was determined to be 14.3 mg/g and 31.3 mg/g respectively for 25°C. The thermodynamic studies indicated that the ΔG for the sorption of As(III) and As(V) ranged from −115.5 to −0.96 kJ/mol, indicating a spontaneous process was occurring. The enthalpy indicated that an exothermic reaction was occurring during the adsorption in which the ΔH was −53.69 kJ/mol and −32.51 kJ/mol for As(III) and As(V) respectively. In addition, ΔS values for the reaction had negative values of −160.46 J/K and −99.77 J/K for the adsorption of As(III) and As(V) respectively which indicated that the reaction was spontaneous at low temperatures. Furthermore, the sorption for As(III) and As(V) was determined to follow the second order kinetics adsorption model
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