335 research outputs found
Development of pyrimidone D1 dopamine receptor positive allosteric modulators
MLS1082 is a structurally novel pyrimidone-based D1-like dopamine receptor positive allosteric modulator. Potentiation of D1 dopamine receptor (D1R) signaling is a therapeutic strategy for treating neurocognitive disorders. Here, we investigate the relationship between D1R potentiation and two prominent structural features of MLS1082, namely the pendant N-aryl and C-alkyl groups on the pyrimidone ring. To this end, we synthesized 24 new analogues and characterized their ability to potentiate dopamine signaling at the D1R and the closely related D5R. We identified structure–activity relationship trends for both aryl and alkyl modifications and our efforts afforded several analogues with improvements in activity. The most effective analogues demonstrated an approximately 8-fold amplification of dopamine-mediated D1R signaling. These findings advance the understanding of structural moieties underlying the activity of pyrimidone-based D1R positive allosteric modulators
Semi-empirical catalog of early-type galaxy-halo systems: dark matter density profiles, halo contraction and dark matter annihilation strength
With SDSS galaxy data and halo data from up-to-date N-body simulations we
construct a semi-empirical catalog (SEC) of early-type systems by making a
self-consistent bivariate statistical match of stellar mass (M_star) and
velocity dispersion (sigma) with halo virial mass (M_vir). We then assign
stellar mass profile and velocity dispersion profile parameters to each system
in the SEC using their observed correlations with M_star and sigma.
Simultaneously, we solve for dark matter density profile of each halo using the
spherical Jeans equation. The resulting dark matter density profiles deviate in
general from the dissipationless profile of NFW or Einasto and their mean inner
density slope and concentration vary systematically with M_vir. Statistical
tests of the distribution of profiles at fixed M_vir rule out the null
hypothesis that it follows the distribution predicted by N-body simulations for
M_vir ~< 10^{13.5-14.5} M_solar. These dark matter profiles imply that dark
matter density is, on average, enhanced significantly in the inner region of
halos with M_vir ~< 10^{13.5-14.5} M_solar supporting halo contraction. The
main characteristics of halo contraction are: (1) the mean dark matter density
within the effective radius has increased by a factor varying systematically up
to ~ 3-4 at M_vir = 10^{12} M_solar, and (2) the inner density slope has a mean
of ~ 1.3 with rho(r) ~ r^{-alpha} and a halo-to-halo rms scatter of
rms(alpha) ~ 0.4-0.5 for 10^{12} M_solar ~< M_vir ~< 10^{13-14} M_solar steeper
than the NFW profile (alpha=1). Based on our results we predict that halos of
nearby elliptical and lenticular galaxies can, in principle, be promising
targets for gamma-ray emission from dark matter annihilation.Comment: 43 pages, 20 figures, JCAP, revised and accepted versio
The reproductive capacity of Monk Parakeets Myiopsitta monachus is higher in their invasive range
Breeding parameters for Monk Parakeets Myiopsitta monachus nesting in Barcelona, Spain, were collected for 651 nests over five breeding seasons. This invasive population has a high reproductive capacity compared with the species in the native range: fledging success was double, the percentage of pairs attempting second broods three times higher and 55% of first-year birds bred compared with almost zero in South America
Constraining Running Non-Gaussianity
The primordial non-Gaussian parameter fNL has been shown to be
scale-dependent in several models of inflation with a variable speed of sound.
Starting from a simple ansatz for a scale-dependent amplitude of the primordial
curvature bispectrum for two common phenomenological models of primordial
non-Gaussianity, we perform a Fisher matrix analysis of the bispectra of the
temperature and polarization of the Cosmic Microwave Background (CMB) radiation
and derive the expected constraints on the parameter nNG that quantifies the
running of fNL(k) for current and future CMB missions such as WMAP, Planck and
CMBPol. We find that CMB information alone, in the event of a significant
detection of the non-Gaussian component, corresponding to fNL = 50 for the
local model and fNL = 100 for the equilateral model of non-Gaussianity, is able
to determine nNG with a 1-sigma uncertainty of Delta nNG = 0.1 and Delta nNG =
0.3, respectively, for the Planck mission. In addition, we consider a Fisher
matrix analysis of the galaxy power spectrum to determine the expected
constraints on the running parameter nNG for the local model and of the galaxy
bispectrum for the equilateral model from future photometric and spectroscopic
surveys. We find that, in both cases, large-scale structure observations should
achieve results comparable to or even better than those from the CMB, while
showing some complementarity due to the different distribution of the
non-Gaussian signal over the relevant range of scales. Finally, we compare our
findings to the predictions on the amplitude and running of non-Gaussianity of
DBI inflation, showing how the constraints on a scale-dependent fNL(k)
translate into constraints on the parameter space of the theory.Comment: 37 pages, 14 figure
Entropic force approach to noncommutative Schwarzschild black holes signals a failure of current physical ideas
Recently, a new perspective of gravitational-thermodynamic duality as an
entropic force arising from alterations in the information connected to the
positions of material bodies is found. In this paper, we generalize some
aspects of this model in the presence of noncommutative Schwarzschild black
hole by applying the method of coordinate coherent states describing smeared
structures. We implement two different distributions: (a) Gaussian and (b)
Lorentzian. Both mass distributions prepare the similar quantitative aspects
for the entropic force. Our study shows, the entropic force on the smallest
fundamental unit of a holographic screen with radius vanishes. As a
result, black hole remnants are unconditionally inert even gravitational
interactions do not exist therein. So, a distinction between gravitational and
inertial mass in the size of black hole remnant is observed, i.e. the failure
of the principle of equivalence. In addition, if one considers the screen
radius to be less than the radius of the smallest holographic surface at the
Planckian regime, then one encounters some unusual dynamical features leading
to gravitational repulsive force and negative energy. On the other hand, the
significant distinction between the two distributions is conceived to occur
around , and that is worth of mentioning: at this regime either our
analysis is not the proper one, or non-extensive statistics should be employed.Comment: 15 pages, 2 figures, new references added, minor revision, Title
changed, to appear in EPJ Plu
The impact of the metabotropic glutamate receptor and other gene family interaction networks on autism
Although multiple reports show that defective genetic networks underlie the aetiology of autism, few have translated into pharmacotherapeutic opportunities. Since drugs compete with endogenous small molecules for protein binding, many successful drugs target large gene families with multiple drug binding sites. Here we search for defective gene family interaction networks (GFINs) in 6,742 patients with the ASDs relative to 12,544 neurologically normal controls, to find potentially druggable genetic targets. We find significant enrichment of structural defects (P≤2.40E-09, 1.8-fold enrichment) in the metabotropic glutamate receptor (GRM) GFIN, previously observed to impact attention deficit hyperactivity disorder (ADHD) and schizophrenia. Also, the MXD-MYC-MAX network of genes, previously implicated in cancer, is significantly enriched (P≤3.83E-23, 2.5-fold enrichment), as is the calmodulin 1 (CALM1) gene interaction network (P≤4.16E-04, 14.4-fold enrichment), which regulates voltage-independent calcium-activated action potentials at the neuronal synapse. We find that multiple defective gene family interactions underlie autism, presenting new translational opportunities to explore for therapeutic interventions
The BRCA2 c.68-7T > A variant is not pathogenic : A model for clinical calibration of spliceogenicity
Although the spliceogenic nature of the BRCA2 c.68-7T > A variant has been demonstrated, its association with cancer risk remains controversial. In this study, we accurately quantified by real-time PCR and digital PCR (dPCR), the BRCA2 isoforms retaining or missing exon 3. In addition, the combined odds ratio for causality of the variant was estimated using genetic and clinical data, and its associated cancer risk was estimated by case-control analysis in 83,636 individuals. Co-occurrence in trans with pathogenic BRCA2 variants was assessed in 5,382 families. Exon 3 exclusion rate was 4.5-fold higher in variant carriers (13%) than controls (3%), indicating an exclusion rate for the c.68-7T > A allele of approximately 20%. The posterior probability of pathogenicity was 7.44x10(-115). There was neither evidence for increased risk of breast cancer (OR 1.03; 95% CI 0.86-1.24) nor for a deleterious effect of the variant when co-occurring with pathogenic variants. Our data provide for the first time robust evidence of the nonpathogenicity of the BRCA2 c.68-7T > A. Genetic and quantitative transcript analyses together inform the threshold for the ratio between functional and altered BRCA2 isoforms compatible with normal cell function. These findings might be exploited to assess the relevance for cancer risk of other BRCA2 spliceogenic variants.Peer reviewe
No evidence that protein truncating variants in BRIP1 are associated with breast cancer risk: implications for gene panel testing.
BACKGROUND: BRCA1 interacting protein C-terminal helicase 1 (BRIP1) is one of the Fanconi Anaemia Complementation (FANC) group family of DNA repair proteins. Biallelic mutations in BRIP1 are responsible for FANC group J, and previous studies have also suggested that rare protein truncating variants in BRIP1 are associated with an increased risk of breast cancer. These studies have led to inclusion of BRIP1 on targeted sequencing panels for breast cancer risk prediction. METHODS: We evaluated a truncating variant, p.Arg798Ter (rs137852986), and 10 missense variants of BRIP1, in 48 144 cases and 43 607 controls of European origin, drawn from 41 studies participating in the Breast Cancer Association Consortium (BCAC). Additionally, we sequenced the coding regions of BRIP1 in 13 213 cases and 5242 controls from the UK, 1313 cases and 1123 controls from three population-based studies as part of the Breast Cancer Family Registry, and 1853 familial cases and 2001 controls from Australia. RESULTS: The rare truncating allele of rs137852986 was observed in 23 cases and 18 controls in Europeans in BCAC (OR 1.09, 95% CI 0.58 to 2.03, p=0.79). Truncating variants were found in the sequencing studies in 34 cases (0.21%) and 19 controls (0.23%) (combined OR 0.90, 95% CI 0.48 to 1.70, p=0.75). CONCLUSIONS: These results suggest that truncating variants in BRIP1, and in particular p.Arg798Ter, are not associated with a substantial increase in breast cancer risk. Such observations have important implications for the reporting of results from breast cancer screening panels.The COGS project is funded through a European Commission's Seventh Framework Programme grant
(agreement number 223175 - HEALTH-F2-2009-223175). BCAC is funded by Cancer Research UK
[C1287/A10118, C1287/A12014] and by the European Community´s Seventh Framework Programme under
grant agreement number 223175 (grant number HEALTH-F2-2009-223175) (COGS). Funding for the iCOGS
infrastructure came from: the European Community's Seventh Framework Programme under grant agreement
n° 223175 (HEALTH-F2-2009-223175) (COGS), Cancer Research UK (C1287/A10118, C1287/A 10710,
C12292/A11174, C1281/A12014, C5047/A8384, C5047/A15007, C5047/A10692, C8197/A16565), the
National Institutes of Health (CA128978) and Post-Cancer GWAS initiative (1U19 CA148537, 1U19
16
CA148065 and 1U19 CA148112 - the GAME-ON initiative), the Department of Defense (W81XWH-10-1-
0341), the Canadian Institutes of Health Research (CIHR) for the CIHR Team in Familial Risks of Breast
Cancer, Komen Foundation for the Cure, the Breast Cancer Research Foundation, and the Ovarian Cancer
Research Fund. This study made use of data generated by the Wellcome Trust Case Control consortium.
Funding for the project was provided by the Wellcome Trust under award 076113. The results published here
are in part based upon data generated by The Cancer Genome Atlas Project established by the National Cancer
Institute and National Human Genome Research Institute.This is the author accepted manuscript. The final version is available from BMJ Group at http://dx.doi.org/10.1136/jmedgenet-2015-103529
Fine-Scale Mapping of the 4q24 Locus Identifies Two Independent Loci Associated with Breast Cancer Risk
Background: A recent association study identified a common variant (rs9790517) at 4q24 to be associated with breast cancer risk. Independent association signals and potential functional variants in this locus have not been explored.
Methods: We conducted a fine-mapping analysis in 55,540 breast cancer cases and 51,168 controls from the Breast Cancer Association Consortium.
Results: Conditional analyses identified two independent association signals among women of European ancestry, represented by rs9790517 [conditional P = 2.51 × 10−4; OR, 1.04; 95% confidence interval (CI), 1.02–1.07] and rs77928427 (P = 1.86 × 10−4; OR, 1.04; 95% CI, 1.02–1.07). Functional annotation using data from the Encyclopedia of DNA Elements (ENCODE) project revealed two putative functional variants, rs62331150 and rs73838678 in linkage disequilibrium (LD) with rs9790517 (r2 ≥ 0.90) residing in the active promoter or enhancer, respectively, of the nearest gene, TET2. Both variants are located in DNase I hypersensitivity and transcription factor–binding sites. Using data from both The Cancer Genome Atlas (TCGA) and Molecular Taxonomy of Breast Cancer International Consortium (METABRIC), we showed that rs62331150 was associated with level of expression of TET2 in breast normal and tumor tissue.
Conclusion: Our study identified two independent association signals at 4q24 in relation to breast cancer risk and suggested that observed association in this locus may be mediated through the regulation of TET2.
Impact: Fine-mapping study with large sample size warranted for identification of independent loci for breast cancer risk
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