Un cas de panostéite chez un Cairn Terrier

La panostéite est une affection osseuse idiopathique du chien en croissance touchant habituellement les races de grande taille. Elle atteint préférentiellement les os longs. Elle se manifeste cliniquement par une boiterie, une douleur à la palpation diaphysaire et radiologiquement par des plages d’ossification intramédullaire. L’observation chez un chiot Cairn Terrier est apparue particulièrement intéressante du fait de sa rareté

HASAR : mining sequential association rules for atherosclerosis risk factor analysis

International audienceWe present the HASAR method that is an hybrid approach for ex- tracting adaptive sequential association rules. This method extracts association rules between events occurring in subsequent time-intervals using closed itemsets extraction and evolutionary techniques. An important feature is its capacity to consider different time-intervals depending on the attributes semantic. We applied this method for the analysis of long term medical observations of atherosclerosis risk factors for cardio-vascular diseases prevention. Experimental results show that it is well-suited for extracting knowledge from temporal data where interesting patterns have different observation period length.Nous présentons la méthode HASAR qui est une approche hybride pour l’extraction de règles d’assocations séquentilles. Cette méthode extrait des règles association entre des évènements ayant lieu à différent moment en extrayant des itemsets fermés fréquents et en utilisant des techniques évolutionnaires. Une fonctionnalité important de notre méthode est sa capacité à s’adapter à différente échelle de temps en fonction de la sémantique des attributs. Nous avons appliqué cette méthode pour analyser des observations médicales à long terme sur les facteurs de risques de l’artherosclerose. Les résultats expérimentaux ont montré que cette méthode est bien adaptée pour extraire des connaissance à partir de données temporelles ou les motifs intéressants doivent être observés sur différentes périodes de temps

KTA : a framework for integrating expert knowledge and experiment memory in transcriptome analysis

International audienceThis paper addresses the problem of the integration of expert knowledge in a data mining process. We present the KTA ( integrating expert Knowledge in Transcriptome analysis) framework which allows the mining process to be driven by prior knowledge on the application domain. KTA is embedded in the MEDIANTE project for evaluating and using DNA microarrays, the CORESE semantic search engine and the ANNOT module which annotates scientific publications.Cet article propose une solution au problème de l’intégration de la connaissance experte dans le processus de data mining. Nous présentons KTA (integrating expert Knowledge in Transcriptome Analysis) qui permet au processus de data mining d’être conduit par des connaissances à priori sur le domaine d’application. KTA est une composante du projet MEDIANTE dont le but est d’évaluer et utiliser des biopuces à ADN

Active Control of Silicon Nanotweezers Detects Enzymatic Reaction at the Molecular Level

International audienceThis work achieved the control of micromachined tweezers for the enhancement of the sensing of DNA molecules and related enzymatic reactions. The mechanical stiffness of the silicon nanotweezers is decreased by feedback design and the sensitivity of the system is drastically improved

Closed-loop Control of Silicon Nanotweezers for Improvement of Sensitivity to Mechanical Stiffness Measurement and Bio-Sensing on DNA Molecules

International audienceIn this work we show that implementation of closed loop control to silicon nanotweezers improves the sensitivity of the tool for mechanical characterizations of biological molecules. Micromachined tweezers have already been used for the characterizations of mechanical properties of DNA molecules as well as for the sensing of enzymatic reactions on DNA bundle. However the resolution of the experiments does not allow the sensing on single molecules. Hereafter we show theoretically and experimentally that, reducing the resonance frequency of the system by the implementation of a state feedback, the sensitivity to stiffness variation is enhanced. Such improvement leads to better resolution for detection of enzymatic reactions on DNA

Measuring currents, ice drift, and waves from space: the Sea Surface KInematics Multiscale monitoring (SKIM) concept

We propose a new satellite mission that uses a near-nadir Ka-band Doppler radar to measure surface currents, ice drift and ocean waves at spatial scales of 40?km and more, with snapshots at least every day for latitudes 75 to 82, and every few days otherwise. The use of incidence angles at 6 and 12 degrees allows a measurement of the directional wave spectrum which yields accurate corrections of the wave-induced bias in the current measurements. The instrument principle, algorithm for current velocity and mission performance are presented here. The proposed instrument can reveal features on tropical ocean and marginal ice zone dynamics that are inaccessible to other measurement systems, as well as a global monitoring of the ocean mesoscale that surpasses the capability of today?s nadir altimeters. Measuring ocean wave properties facilitates many applications, from wave-current interactions and air-sea fluxes to the transport and convergence of marine plastic debris and assessment of marine and coastal hazards

Long-term outcome in relationship to neonatal transfusion volume in extremely premature infants: a comparative cohort study

<p>Abstract</p> <p>Background</p> <p>In premature born infants red blood cell (RBC) transfusions have been associated with both beneficial and detrimental sequels. Upon RBC transfusion, improvement in cerebral blood flow and oxygenation have been observed, while a more liberal transfusion policy may be associated with a better developmental outcome. The effect of the transfusion volume on long-term outcome is not known.</p> <p>Methods</p> <p>Observational follow-up study of a cohort of extremely premature born infants, treated in 2 neonatal intensive care units using a different transfusion volume (15 ml/kg in Unit A and 20 ml/kg in Unit B). The primary outcome was a composite of post discharge mortality, neuromotor developmental delay, blindness or deafness, evaluated at a mean corrected age (CA) of 24 months related to the transfusion volume/kg bodyweight administered during the postnatal hospital stay.</p> <p>Results</p> <p>Despite the difference in transfusion volume in clinically comparable groups of infants, they received a similar number of transfusions (5.5 ± 3.2 versus 5.5 ± 2.3 respectively in Unit A and B). The total transfused volume in unit A was 79 ± 47 ml/kg and 108 ± 47 ml/kg in unit B (p = 0.02). Total transfused RBC volume per kg bodyweight was not an independent predictor of the composite outcome (p = 0.96, OR 1.0 (CI 0.9-1.1).</p> <p>Conclusion</p> <p>There was no relationship between the composite outcome at 24 months CA and transfusion volume received during the post natal hospital stay. As there was no clinical advantage of the higher transfusion volume, a more restrictive volume will reduce total transfusion volume and donor exposure. Future research on the optimal transfusion volume per event to extreme preterm infants should include larger, prospective studies with a longer follow-up period through to childhood or even adolescence.</p

Biodistribution, metabolism, and in vivo gene expression of low molecular weight glycopeptide polyethylene glycol peptide DNA co-condensates

The biodistribution, metabolism, cellular targeting, and gene expression of a nonviral peptide DNA gene delivery system was examined. 125 I-labeled plasmid DNA was condensed with low molecular weight peptide conjugates and dosed i.v. in mice to determine the influence of peptide DNA formulation parameters on specific gene targeting to hepatocytes. Optimal targeting to hepatocytes required the combined use of a triantennary glycopeptide (Tri-CWK 18 ) and a polyethylene glycol-peptide (PEG-CWK 18 ) to mediate specific recognition by the asialoglycoprotein receptor and to reduce nonspecific uptake by Kupffer cells. Tri-CWK 18 /PEG-CWK 18 DNA co-condensates were stabilized and protected from metabolism by glutaraldehyde crosslinking. An optimized formulation targeted 60% of the dose to the liver with 80% of the liver targeted DNA localized to hepatocytes. Glutaraldehyde crosslinking of DNA condensates reduced the liver elimination rate from a t ½ of 0.8 to 3.6 h. An optimized gene delivery formulation produced detectable levels of human Α1-antitrypsin in mouse serum which peaked at day 7 compared to no expression using control formulations. The results demonstrate the application of formulation optimization to improve the targeting selectivity and gene expression of a peptide DNA delivery system. © 2000 Wiley-Liss, Inc. and the American Pharmaceutical Association J Pharm Sci 89: 499–512, 2000Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/34500/1/7_ftp.pd