Cuando se apaga el semáforo (Reportaje)

En España, el motociclismo no viene parejo exclusivamente con el Campeonato del Mundo de MotoGP. A su espalda converge otro campeonato mundial con grandes estrellas del motor, el Mundial de Superbike. Sin embargo, su popularidad es mayor en países europeos como Italia o Gran Bretaña. Para que España se sumara al interés por las motos derivadas de serie era necesaria la llegada de un piloto español de la élite de este deporte. Por tanto, el objetivo principal de nuestro reportaje se centra en hacer un recorrido de la trayectoria de un piloto español, Álvaro Bautista, desde que pone fin a su carrera deportiva en MotoGP hasta su llegada al campeonato de Superbike. Una competición que con su aterrizaje parece dejar de ser desconocida en España.In Spain, motorcycling does not come exclusively with the MotoGP World Championship. Behind it converges another world championship with great motor stars, World Superbike Championship. However, its popularity is greater in European countries such as Italy or Great Britain. For Spain to join the interest in production bikes, the arrival of a Spanish rider from the elite of this sport was necessary. Therefore, the main objective of our report is to take a look at the career of a Spanish rider, Álvaro Bautista, from the end of his MotoGP career until his arrival to the Superbike championship. A championship that with his landing seems to be no longer unknown in Spain.Universidad de Sevilla. Grado en Periodism

Is Job Involvement Enough for Achieving Job Satisfaction? The Role of Skills: Use and Group Identification

The main objective of this research is to evaluate the influence of job involvement over job satisfaction mediated through the professional skill use and moderated by group identification. The sample of the current research was composed of 420 subjects. The main results showed that job involvement was strongly related to skill use and group identification. Moreover, the interaction of job involvement and group identification is negatively related with skill use. Our results show that there is no statistically significant relationship between job involvement and job satisfaction. Furthermore, the use of skills is strongly related to job satisfaction. Lastly, we found that a strong group identification tends to harm job satisfaction values.2020-2

Reyes a pie de calle. Simbolismo y espacio público en Europa meridional (siglos XIX y XX). Presentación

Presentación del Dossier "Reyes a pie de calle" por parte de sus coordinadore

Botanical composition of sheep diet in two contrasting environments at Tierra del Fuego steppe (Argentina)

The botanical composition of the diet of sheep in two contrasting environments of the fueguian steppe was compared by means of a microscopic analysis of feces, during the spring and summer 2006. In both areas of study, grasses showed to be the most important group in the diet of both locations and of both seasons, followed by native dicotyledonous. In addition, it was found that in the abandoned pasture of Cullen ranch, the intake of Hieracium pilosella, an invasive species, is high. Management strategies should be targeted to the conservation of grasses which are the life forms that are most susceptible to over grazing disturbance. Besides, these results contribute to the stage of advancement of the invasive species H. pilosella in the fueguian steppe and to the knowledge of the incorporation of this species in the diet of livestock, and possibly in the diets of native herbivores.Fil: Fernández Pepi, María Gabriela. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Nutrición Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Arriaga, Mirta Olga. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Stampacchio, Monica Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; ArgentinaFil: Collantes, Marta Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Museo Argentino de Ciencias Naturales "Bernardino Rivadavia"; Argentin

Analytical exploratory tool for healthcare professionals to monitor cancer patients' progress

[EN] Cancer is a primary public concern in the European continent. Due to the large case numbers and survival rates, a significant population is living with cancer needs. Consequently, health professionals must deal with complex treatment decision-making processes. In this context, much data is collected during cancer care delivery. Once collected, these data are complex for health professionals to access to support clinical decision-making and performance review. There is a need for innovative tools that make clinical data more accessible to support cancer health professionals in these activities. One approach for providing health professionals with access to clinical data is to create the infrastructure and interface for a clinical tool to make data accessible in a relevant manner. In this sense, results should be understandable and valuable for cancer experts to evaluate and optimize cancer processes. This work aims to collect and report the process of developing an exploratory analytical Interactive Process Mining tool with clinical relevance for healthcare professionals for monitoring cancer patients¿ care processes in the context of the LifeChamps project. Following a co-creation and interactive approach thanks to the Interactive Process Mining paradigm, the tool presents patients¿ progress over time for different clinical models and a graphical and navigable Process Indicator in the context of prostate cancer patients.This work was partially funded by the European Union¿s Horizon 2020 research and innovation program under Grant Agreement No 875329.Valero Ramon, Z.; Fernández Llatas, C.; Collantes, G.; Valdivieso, F.; Billis, A.; Bamidis, P.; Traver Salcedo, V. (2023). Analytical exploratory tool for healthcare professionals to monitor cancer patients' progress. Frontiers in Oncology. 12:1-19. https://doi.org/10.3389/fonc.2022.10434111191

Integrative transcriptome and proteome analyses define marked differences between Neospora caninum isolates throughout the tachyzoite lytic cycle

Neospora caninum is one of the main causes of transmissible abortion in cattle. Intraspecific variations in virulence have been widely shown among N. caninum isolates. However, the molecular basis governing such variability have not been elucidated to date. In this study label free LC-MS/MS was used to investigate proteome differences between the high virulence isolate Nc-Spain7 and the low virulence isolate Nc-Spain1H throughout the tachyzoite lytic cycle. The results showed greater differences in the abundance of proteins at invasion and egress with 77 and 62 proteins, respectively. During parasite replication, only 19 proteins were differentially abundant between isolates. The microneme protein repertoire involved in parasite invasion and egress was more abundant in the Nc-Spain1H isolate, which displays a lower invasion rate. Rhoptry and dense granule proteins, proteins related to metabolism and stress responses also showed differential abundances between isolates. Comparative RNA-Seq analyses during tachyzoite egress were also performed, revealing an expression profile of genes associated with the bradyzoite stage in the low virulence Nc-Spain1H isolate. The differences in proteome and RNA expression profiles between these two isolates reveal interesting insights into likely mechanisms involved in specific phenotypic traits and virulence in N. caninum. Significance The molecular basis that governs biological variability in N. caninum and the pathogenesis of neosporosis has not been well-established yet. This is the first study in which high throughput technology of LC-MS/MS and RNA-Seq is used to investigate differences in the proteome and transcriptome between two well-characterized isolates. Both isolates displayed different proteomes throughout the lytic cycle and the transcriptomes also showed marked variations but were inconsistent with the proteome results. However, both datasets identified a pre-bradyzoite status of the low virulence isolate Nc-Spain1H. This study reveals interesting insights into likely mechanisms involved in virulence in N. caninum and shed light on a subset of proteins that are potentially involved in the pathogenesis of this parasite

Comparative performance evaluation of commercial packing materials for malodorants abatement in biofiltration

Producción CientíficaPacking materials used in biofiltration of gaseous pollutants represent a key design parameter, as a proper selection might not only determine the adequate performance of the system but also its cost-effectiveness. This study systematically assessed and compared the performance of a conventional plastic carrier with that of two novel clay-based materials from SAINT GOBAIN for the abatement of a model odorous stream composed of H2S, methylmercaptan and toluene. The packing materials were tested in biotrickling filters, biofilters and a two-phase biotrickling filter. SAINT GOBAIN materials exhibited a higher adsorption potential under abiotic conditions, a higher buffer capacity and a superior performance compared to conventional plastic rings when implemented in biotrickling filters operating at gas residence times as low as 7.5 s. Among the materials tested in biofilters, Filtralite Air AC supported almost complete H2S and toluene removals at a gas residence time of 20 s, while successfully eliminating methylmercaptan at values of ~80%. Interestingly, under most of the conditions tested, clay-based materials also showed comparable pressure drop values than those of plastic rings.Junta de Castilla y León - (grant CLU 2017-09

Isolation and genetic characterization of Toxoplasma gondii in Spanish sheep flocks

[EN] Background: Toxoplasma gondii is a major cause of abortion in small ruminants and presents a zoonotic risk when undercooked meat containing cysts is consumed. The aim of the present study was to investigate the genetic diversity among the T. gondii strains circulating in ovine livestock in Spain. Methods: Selected samples collected from abortion outbreaks due to toxoplasmosis (n = 31) and from chronically infected adult sheep at slaughterhouses (n = 50) in different Spanish regions were bioassayed in mice, aiming at parasite isolation. In addition, all original clinical samples and the resulting isolates were genotyped by multi-nested PCR-RFLP analysis of 11 molecular markers and by PCR-DNA sequencing of portions of the SAG3, GRA6 and GRA7 genes. Results: As a result, 30 isolates were obtained from 9 Spanish regions: 10 isolates from abortion-derived samples and 20 isolates from adult myocardial tissues. Overall, 3 genotypes were found: ToxoDB#3 (type II PRU variant) in 90% (27/30) of isolates, ToxoDB#2 (clonal type III) in 6.7% (2/30), and ToxoDB#1 (clonal type II) in 3.3% (1/30). When T. gondii-positive tissue samples (n = 151) were directly subjected to RFLP genotyping, complete restriction profiles were obtained for 33% of samples, and up to 98% of the specimens belonged to the type II PRU variant. A foetal brain showed a clonal type II pattern, and four specimens showed unexpected type I alleles at the SAG3 marker, including two foetal brains that showed I + II alleles as co-infection events. Amplicons of SAG3, GRA6 and GRA7 obtained from isolates and clinical samples were subjected to sequencing, allowing us to confirm RFLP results and to detect different single-nucleotide polymorphisms. Conclusions: The present study informed the existence of a predominant type II PRU variant genotype (ToxoDB#3) infecting domestic sheep in Spain, in both abortion cases and chronic infections in adults, coexisting with other clonal (ToxoDB#1 and ToxoDB#2), much less frequent genotypes, as well as polymorphic strains as revealed by clinical sample genotyping. The use of multilocus sequence typing aided in accurately estimating T. gondii intragenotype diversitySIThis research was supported by projects funded by the Spanish Ministry of Science and Innovation (AGL2016-75935-C2-R) and the Community of Madrid (PLATESA2-CM-P2018/BAA-4370). MF and RC were funded by UCMSantander/ 2017 pre-doctoral grants, and PLATESA2 post-doctoral grants, respectively. CG was funded by DGAPA, National Autonomous University of Mexico (UNAM). RC, EC and LO are part of the TOXOSOURCES consortium, supported by funding from the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No. 773830: One Health European Joint Programm

Isolation and genetic characterization of Toxoplasma gondii in Spanish sheep flocks

© The Author(s) 2020.[Background]: Toxoplasma gondii is a major cause of abortion in small ruminants and presents a zoonotic risk when undercooked meat containing cysts is consumed. The aim of the present study was to investigate the genetic diversity among the T. gondii strains circulating in ovine livestock in Spain.[Methods]: Selected samples collected from abortion outbreaks due to toxoplasmosis (n = 31) and from chronically infected adult sheep at slaughterhouses (n = 50) in different Spanish regions were bioassayed in mice, aiming at parasite isolation. In addition, all original clinical samples and the resulting isolates were genotyped by multi-nested PCR-RFLP analysis of 11 molecular markers and by PCR-DNA sequencing of portions of the SAG3, GRA6 and GRA7 genes.[Results]: As a result, 30 isolates were obtained from 9 Spanish regions: 10 isolates from abortion-derived samples and 20 isolates from adult myocardial tissues. Overall, 3 genotypes were found: ToxoDB#3 (type II PRU variant) in 90% (27/30) of isolates, ToxoDB#2 (clonal type III) in 6.7% (2/30), and ToxoDB#1 (clonal type II) in 3.3% (1/30). When T. gondii-positive tissue samples (n = 151) were directly subjected to RFLP genotyping, complete restriction profiles were obtained for 33% of samples, and up to 98% of the specimens belonged to the type II PRU variant. A foetal brain showed a clonal type II pattern, and four specimens showed unexpected type I alleles at the SAG3 marker, including two foetal brains that showed I + II alleles as co-infection events. Amplicons of SAG3, GRA6 and GRA7 obtained from isolates and clinical samples were subjected to sequencing, allowing us to confirm RFLP results and to detect different single-nucleotide polymorphisms.[Conclusions]: The present study informed the existence of a predominant type II PRU variant genotype (ToxoDB#3) infecting domestic sheep in Spain, in both abortion cases and chronic infections in adults, coexisting with other clonal (ToxoDB#1 and ToxoDB#2), much less frequent genotypes, as well as polymorphic strains as revealed by clinical sample genotyping. The use of multilocus sequence typing aided in accurately estimating T. gondii intragenotype diversity.This research was supported by projects funded by the Spanish Ministry of Science and Innovation (AGL2016-75935-C2-R) and the Community of Madrid (PLATESA2-CM-P2018/BAA-4370). MF and RC were funded by UCM-Santander/2017 pre-doctoral grants, and PLATESA2 post-doctoral grants, respectively. CG was funded by DGAPA, National Autonomous University of Mexico (UNAM). RC, EC and LO are part of the TOXOSOURCES consortium, supported by funding from the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No. 773830: One Health European Joint Programme.Peer reviewe

Experimental infection with a low virulence isolate of Neospora caninum at 70 days gestation in cattle did not result in foetopathy

The Nc-Spain 1H isolate of Neospora caninum, which was newly obtained from the brain of a congenitally asymptomatic infected calf, demonstrated a reduced in vitro tachyzoite yield and viability rate, as well as low virulence in mouse models. The objective of the present study was to determine the ability of this isolate to induce foetal death in a pregnant bovine model. For this purpose, 13 naïve pregnant heifers were divided into three groups and were experimentally challenged with either 107 tachyzoites of Nc-1 (group 1, n = 5), Nc-Spain 1H (group 2, n = 5) isolates or phosphate-buffered saline (group 3, n = 3) intravenously at 70 days of gestation. After inoculation, pregnancy was monitored and dams were sacrificed when foetal death was detected. The remaining animals were slaughtered at 45 days post-infection. Maternal and foetal samples were collected for examination by histology and parasite DNA detection. Parasitaemia, specific anti-N. caninum IgG and interferon γ responses were also studied. At 3–4 weeks after infection, foetal death was detected in 3 out of 5 Nc-1-infected dams. However, no evidence of foetal death was observed in either Nc-Spain 1H-infected or control groups during the period studied. The most severe histopathological lesions were observed in the placenta and foetal organs from Nc-1-infected cattle that exhibited foetal death. It was in these animals that N. caninum DNA was more frequently detected. Parasitaemia was observed in all Nc-1-infected dams and in only 3 out of 5 Nc-Spain 1H-infected animals. The magnitude of the immune response was significantly higher in the Nc-1-inoculated group than in the group inoculated with the Nc-Spain 1H isolate. These data reveal the reduced virulence of the Nc-Spain 1H isolate in cattle