Psoriasis therapy and aortic inflammation — translating statistical to clinical significance

Psoriasis patients are known to have comorbid aortic vascular inflammation, which is one of the leading causes of cardiovascular morbidity and mortality in this population. Many studies report statistically significant improvements in aortic vascular inflammation after use of tumor necrosis factor inhibitors or interleukin-12/23 antagonists. However, the clinical significance in reduction of adverse cardiovascular events in psoriatic patients owing to biologic therapy has not been examined. Regardless of clinically significant cardiovascular benefits, dermatologists should continue to treat psoriasis patients optimally to mitigate the unfavorable effect this disease has on quality of life

Internet-based survey intervention improves adherence to methotrexate among psoriasis patients

Background While it is known that psoriasis patients have poor adherence to both topical and systemic medications, adherence to methotrexate is not well-characterized, and ways to improve methotrexate adherence have not been addressed. Objective The aim of this study was to evaluate whether a digital intervention improved adherence to oral methotrexate as measured by electronic monitoring. Methods Twenty-nine patients were randomized to receive either weekly digital interventions assessing treatment adherence or no intervention for 24 weeks. Patients received medication bottles with electronic monitoring, and returned at weeks 4, 12, and 24 to evaluate disease severity. Results The intervention group took methotrexate correctly 77.1% of the weeks observed compared to the control group which averaged 64.5%. More intervention patients took methotrexate as directed compared to the control group (78.3% vs 64.2%, p < 0.0001). Patients were most adherent around follow-up visits, with 100% of digital intervention patients and 80% of control patients taking methotrexate correctly during the week of a follow-up visit (p = 0.02). The digital intervention did not significantly improve disease severity in the intervention group compared to the nonintervention group. Conclusions Low cost, scalable digital interventions may have the potential to increase psoriasis patient adherence to methotrexate, although the mechanism for the improvement is not yet well defined

Stimulated Release of Cholesterol from Liposomal Membranes by a PEGylated Phospholipid

PEGylated phospholipids are commonly used to increase the blood-circulation time of liposomes by providing a steric barrier around them. This paper documents a fundamentally new property of these lipidsan ability to stimulate the release of cholesterol from phospholipid membranes. Evidence for such stimulation has been obtained by measuring the transport of dehydroergosterol (DHE), a fluorescent simulant of cholesterol, from donor liposomes made from 1-palmitoyl-2-oleoyl-<i>sn</i>-glycero-3-phosphocholine (POPC), 1,2-distearoyl-<i>sn</i>-glycero-3-phosphoethanolamine-<i>N</i>-[methoxy­(polyethylene glycol)-2000 (DSPE-PEG₂₀₀₀), and DHE to acceptor liposomes made from POPC, 1-palmitoyl-2-oleoyl-<i>sn</i>-glycero-3-phosphoglycerol (POPG), and cholesterol. The potential of PEGylated lipids to serve as novel cholesterol-lowering agents is briefly discussed

Theories and Experiments for Testable Baryogenesis Mechanisms: A Snowmass White Paper

The baryon asymmetry of the Universe is one of the central motivations to expect physics beyond the Standard Model. In this Snowmass white paper, we review the challenges and opportunities in testing some of the central paradigms that predict physics at scales low enough to expect new experimental data in the next decade. Focusing on theoretical ideas and some of their experimental implications, in particular, we discuss neutron-antineutron transformations, flavor observables, next generation colliders, future neutron facilities, gravitational waves, searches for permanent electric dipole moments, $0\nu \beta \beta$ decay and some future large underground experiments as methods to test post-sphaleron baryogenesis, electroweak baryogenesis, mesogenesis and low scale leptogenesis. Finally, we comment on the cases where high scale physics can be probed through some of these same mechanisms

Theories and Experiments for Testable Baryogenesis Mechanisms: A Snowmass White Paper

The baryon asymmetry of the Universe is one of the central motivations to expect physics beyond the Standard Model. In this Snowmass white paper, we review the challenges and opportunities in testing some of the central paradigms that predict physics at scales low enough to expect new experimental data in the next decade. Focusing on theoretical ideas and some of their experimental implications, in particular, we discuss neutron-antineutron transformations, flavor observables, next generation colliders, future neutron facilities, gravitational waves, searches for permanent electric dipole moments, $0\nu \beta \beta$ decay and some future large underground experiments as methods to test post-sphaleron baryogenesis, electroweak baryogenesis, mesogenesis and low scale leptogenesis. Finally, we comment on the cases where high scale physics can be probed through some of these same mechanisms

Regulation of genes encoding beta-D-glucan glucohydrolases in barley (Hordeum vulgare)

The definitive version is available at www.blackwell-synergy.comExpression patterns of barley β-D-glucan glucohydrolase genes were monitored using cDNAs encoding isoenzymes ExoI and ExoII. The cDNAs were isolated from 5-day-old seedling libraries. The enzymes are encoded by a small gene family, in which marked differences in codon usage are evident. The cDNAs can be used as specific probes for two subfamilies of β-D-glucan glucohydrolase genes. Genes of both subfamilies are transcribed in the scutellum of germinated grain, in elongating coleoptiles, and in young roots and leaves. Low levels of mRNA for the isoenzyme ExoI gene subfamily could be detected in aleurone layers of germinated grain. Most of the β-D-glucan glucohydrolase activity can be extracted from tissues with dilute aqueous buffers. Enzyme activity is highest in young leaves and elongating coleoptiles, but is not well-correlated with mRNA levels. The expression patterns are consistent with proposed roles for β-glucan glucohydrolases in the turnover or modification of cell-wall (1→3,1→4)-β-D-glucans in elongating coleoptiles and in young vegetative tissues.Andrew J. Harvey, Maria Hrmova, Geoffrey B. Finche