Effect of Caffeine Ingestion on Indirect Markers of Exercise-Induced Muscle Damage: A Systematic Review of Human Trials

The effect of caffeine on mitigating exercise-induced muscle damage (EIMD) is still poorly understood, but it was hypothesized that caffeine could contribute to decreasing delayed onset muscle soreness, attenuating temporary loss of strength, and reducing circulating levels of blood markers of muscle damage. However, evidence is not conclusive and beneficial effects of caffeine ingestion on EIMD are not always observed. Factors, such as the type of exercise that induces muscle damage, supplementation protocol, and type of marker analyzed contribute to the differences between the studies. To expand knowledge on the role of caffeine supplementation in EIMD, this systematic review aimed to investigate the effect of caffeine supplementation on different markers of muscle damage. Fourteen studies were included, evaluating the effect of caffeine on indirect muscle damage markers, including blood markers (nine studies), pain perception (six studies), and MVC maximal voluntary contraction force (four studies). It was observed in four studies that repeated administration of caffeine between 24 and 72 h after muscle damage can attenuate the perception of pain in magnitudes ranging from 3.9% to 26%. The use of a single dose of caffeine pre-exercise (five studies) or post-exercise (one study) did not alter the circulating blood levels of creatine kinase (CK). Caffeine supplementation appears to attenuate pain perception, but this does not appear to be related to an attenuation of EIMD, per se. Furthermore, the effect of caffeine supplementation after muscle damage on strength recovery remains inconclusive due to the low number of studies found (four studies) and controversial results for both dynamic and isometric strength tests

Differentially Testing Soundness and Precision of Program Analyzers

In the last decades, numerous program analyzers have been developed both by academia and industry. Despite their abundance however, there is currently no systematic way of comparing the effectiveness of different analyzers on arbitrary code. In this paper, we present the first automated technique for differentially testing soundness and precision of program analyzers. We used our technique to compare six mature, state-of-the art analyzers on tens of thousands of automatically generated benchmarks. Our technique detected soundness and precision issues in most analyzers, and we evaluated the implications of these issues to both designers and users of program analyzers

Geometry of Frictionless and Frictional Sphere Packings

We study static packings of frictionless and frictional spheres in three dimensions, obtained via molecular dynamics simulations, in which we vary particle hardness, friction coefficient, and coefficient of restitution. Although frictionless packings of hard-spheres are always isostatic (with six contacts) regardless of construction history and restitution coefficient, frictional packings achieve a multitude of hyperstatic packings that depend on system parameters and construction history. Instead of immediately dropping to four, the coordination number reduces smoothly from $z=6$ as the friction coefficient $\mu$ between two particles is increased.Comment: 6 pages, 9 figures, submitted to Phys. Rev.

Low Metallicity Galaxies from the Dark Energy Survey

We present a new selection of 358 blue compact dwarf galaxies (BCDs) from 5,000 square degrees in the Dark Energy Survey (DES), and the spectroscopic follow-up of a subsample of 68 objects. For the subsample of 34 objects with deep spectra, we measure the metallicity via the direct T$_e$ method using the auroral [\oiii]$\lambda$ 4363 emission line. These BCDs have average oxygen abundance of 12+log(O/H)= 7.8, stellar masses between 10$^7$ to 10$^8$ M$_\odot$ and specific SFR between $\sim$ 10$^{-9}$ to 10$^{-7}$ yr$^{-1}$. We compare the position of our BCDs with the Mass-metallicity (M-Z) and Luminosity-metallicity (L-Z) relation derived from the Local Volume Legacy sample. We find the scatter around the M-Z relation is smaller than the scatter around the L-Z relation. We identify a correlation between the offsets from the M-Z and L-Z relation that we suggest is due to the contribution of metal-poor inflows. Finally, we explore the validity of the mass-metallicity-SFR fundamental plane in the mass range probed by our galaxies. We find that BCDs with stellar masses smaller than $10^{8}$M$_{\odot}$ do not follow the extrapolation of the fundamental plane. This result suggests that mechanisms other than the balance between inflows and outflows may be at play in regulating the position of low mass galaxies in the M-Z-SFR space.Comment: 16 pages, 10 figures, submitted to ApJ, We invite comments from the communit

Molecular characterization of the human COQ5 C-methyltransferase in coenzyme Q10 biosynthesis

Under a Creative Commons license.Coq5 catalyzes the only C-methylation involved in the biosynthesis of coenzyme Q (Q or ubiquinone) in humans and yeast Saccharomyces cerevisiae. As one of eleven polypeptides required for Q production in yeast, Coq5 has also been shown to assemble with the multi-subunit complex termed the CoQ-synthome. In humans, mutations in several COQ genes cause primary Q deficiency, and a decrease in Q biosynthesis is associated with mitochondrial, cardiovascular, kidney and neurodegenerative diseases. In this study, we characterize the human COQ5 polypeptide and examine its complementation of yeast coq5 point and null mutants. We show that human COQ5 RNA is expressed in all tissues and that the COQ5 polypeptide is associated with the mitochondrial inner membrane on the matrix side. Previous work in yeast has shown that point mutations within or adjacent to conserved COQ5 methyltransferase motifs result in a loss of Coq5 function but not Coq5 steady state levels. Here, we show that stabilization of the CoQ-synthome within coq5 point mutants or by over-expression of COQ8 in coq5 null mutants permits the human COQ5 homolog to partially restore coq5 mutant growth on respiratory media and Q6 content. Immunoblotting against the human COQ5 polypeptide in isolated yeast mitochondria shows that the human Coq5 polypeptide migrates in two-dimensional blue-native/SDS-PAGE at the same high molecular mass as other yeast Coq proteins. The results presented suggest that human and Escherichia coli Coq5 homologs expressed in yeast retain C-methyltransferase activity but are capable of rescuing the coq5 yeast mutants only when the CoQ-synthome is assembled.Open Access funded by Telethon (Italy).Peer Reviewe

Resolved images of self-gravitating circumstellar discs with ALMA

In this paper we present simulated observations of massive self-gravitating circumstellar discs using the Atacama Large Millimetre/sub-millimetre Array (ALMA). Using a smoothed particle hydrodynamics model of a $0.2M_{\odot}$ disc orbiting a $1M_{\odot}$ protostar, with a cooling model appropriate for discs at temperatures below $\sim 160$K and representative dust opacities, we have constructed maps of the expected emission at sub-mm wavelengths. We have then used the CASA ALMA simulator to generate simulated images and visibilities with various array configurations and observation frequencies, taking into account the expected thermal noise and atmospheric opacities. We find that at 345 GHz (870 $\mu$m) spiral structures at a resolution of a few AU should be readily detectable in approximately face-on discs out to distances of the Taurus-Auriga star-forming complex.Comment: 8 pages, 6 figures, accepted to MNRAS. Figure quality degraded. Full paper with higher quality figures available at http://www2.fisica.unimi.it/lodato/CossinsLodatoTesti1.pd

Radio multiwavelength analysis of the compact disk CX Tau: strong free-free variability or anomalous microwave emission?

Protoplanetary disks emit radiation across a broad range of wavelengths, requiring a multiwavelength approach to fully understand their physical mechanisms and how they form planets. Observations at sub-millimeter to centimeter wavelengths can provide insights into the thermal emission from dust, free-free emission from ionized gas, and possible gyro-synchrotron emission from the stellar magnetosphere. This Letter focuses on CX Tau, a ${\sim}0.4\,M_\odot$ star with an extended gas emission and a compact and apparently structureless dust disk, with an average millimeter flux when compared to Class II sources in Taurus. We present Karl G. Jansky Very Large Array (VLA) observations in 4 bands (between 9.0 mm and 6.0 cm) and combine them with archival data from the Atacama Large Millimeter/submillimeter Array (ALMA), the Submillimeter Array (SMA) and the Plateau de Bure Interferometer (PdBI). Such a multiwavelength approach allows to separate the dust continuum from other emissions. After isolating the dust thermal emission, we derived an upper limit of the dust disk extent at 1.3 cm which is consistent with theoretical predictions of a radial drift-dominated disk. Centimeter data show a peculiar behavior: deep observations at 6.0 cm did not detect the source, while at 1.3 cm the flux density is anomalously higher than adjacent bands. Intraband spectral indices suggest a dominant contribution from free-free emission, whereas gyro-synchrotron emission is excluded. To explain these observations, we propose strong variability of the free-free emission with timescales shorter than a month. Another possible interpretation is the presence of anomalous microwave emission from spinning dust grains.Comment: Accepted for publication in A&A Letters; 13 pages, 13 figures, 1 tabl

Understanding The Impact of Solver Choice in Model-Based Test Generation

Background: In model-based test generation, SMT solvers explore the state-space of the model in search of violations of specified properties. If the solver finds that a predicate can be violated, it produces a partial test specification demonstrating the violation.Aims: The choice of solvers is important, as each may produce differing counterexamples. We aim to understand how solver choice impacts the effectiveness of generated test suites at finding faults.Method: We have performed experiments examining the impact of solver choice across multiple dimensions, examining the ability to attain goal satisfaction and fault detection when satisfaction is achieved---varying the source of test goals, data types of model input, and test oracle.Results: The results of our experiment show that solvers vary in their ability to produce counterexamples, and---for models where all solvers achieve goal satisfaction---in the resulting fault detection of the generated test suites. The choice of solver has an impact on the resulting test suite, regardless of the oracle, model structure, or source of testing goals.Conclusions: The results of this study identify factors that impact fault-detection effectiveness, and advice that could improve future approaches to model-based test generation

Statistics of the contact network in frictional and frictionless granular packings

Simulated granular packings with different particle friction coefficient mu are examined. The distribution of the particle-particle and particle-wall normal and tangential contact forces P(f) are computed and compared with existing experimental data. Here f equivalent to F/F-bar is the contact force F normalized by the average value F-bar. P(f) exhibits exponential-like decay at large forces, a plateau/peak near f = 1, with additional features at forces smaller than the average that depend on mu. Computations of the force-force spatial distribution function and the contact point radial distribution function indicate that correlations between forces are only weakly dependent on friction and decay rapidly beyond approximately three particle diameters. Distributions of the particle-particle contact angles show that the contact network is not isotropic and only weakly dependent on friction. High force-bearing structures, or force chains, do not play a dominant role in these three dimensional, unloaded packings.Comment: 11 pages, 13 figures, submitted to PR