Production and temperature sensitivity of long chain alkenones in the cultured haptophyte Pseudoisochrysis paradoxa

The alkenone unsaturation index (U<sub>37</sub><sup>K</sup> or U<sub>37</sub><sup>K′</sup>) serves as a critical tool for reconstructing temperature in marine environments. Lacustrine haptophyte algae are genetically distinct from their ubiquitous and well studied marine counterparts, and the unknown species-specific genetic imprints on long chain alkenone production by lacustrine species have hindered the widespread application of the U37<sup>K</sup> temperature proxy to lake sediment records. The haptophyte Pseudoisochrysis paradoxa produces alkenones but its U37<sup>K</sup> calibration has never been determined. It has an alkenone fingerprint abundant in tetraunsaturated alkenones, a hallmark of lacustrine environments. We present here the first calibration of the U37<sup>K</sup> index to temperature for a culture of P. paradoxa. We found that the U37<sup>K</sup> index accurately captured the alkenone response to temperature whereas the U37<sup>K′</sup> index failed to do so, with U37<sup>K′</sup> values below 0.08 projecting to two different temperature values. Our results add a fifth species-specific U37<sup>K</sup> calibration and provide another line of evidence that different haptophyte species require different U37<sup>K</sup> calibrations. The findings also highlight the necessary inclusion of the C<sub>37:4</sub> alkenone when reconstructing temperatures from P. paradoxa-derived alkenone records

Culturing of the first 37:4 predominant lacustrine haptophyte : geochemical, biochemical, and genetic implications

Author Posting. © The Author(s), 2011. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Geochimica et Cosmochimica Acta 78 (2012): 51–64, doi:10.1016/j.gca.2011.11.024.Long chain alkenones (LCAs) are potential biomarkers for quantitative paleotemperature reconstructions from lacustrine environments. However, progress in this area has been severely hindered by the lack of culture studies of haptophytes responsible for alkenone distributions in lake sediments: the predominance of C37:4 LCA. Here we report the first enrichment culturing of a novel haptophyte phylotype (Hap-A) from Lake George, ND that produces predominantly C37:4-LCA. Hap-A was enriched from its resting phase collected from deep sediments rather than from water column samples. In contrast, enrichments from near surface water yielded a different haptophyte phylotype (Hap-B), closely related to Chrysotila lamellosa and Pseudoisochrysis paradoxa, which does not display C37:4-LCA predominance (similar enrichments have been reported previously). The LCA profile in sediments resembles that of Hap-A enrichments, suggesting that Hap-A is the dominant alkenone producer of the sedimentary LCAs. In enrichments, excess lighting appeared to be crucial for triggering blooms of Hap-A. Both and indices show a linear relationship with temperature for Hap-A in enrichments, but the relationship appears to be dependent on the growth stage. Based on 18S rRNA gene analyses, several lakes from the Northern Great Plains, as well as Pyramid Lake, NV and Tso Ur, Tibetan Plateau, China contain the same two haptophyte phylotypes. The Great Plains lakes show the Hap-A-type LCA distribution, whereas Pyramid and Tso Ur show the Hap-B type distribution. Waters of the Great Plain lakes are dominated by sulfate, whereas those Pyramid and Tso Ur are dominated by carbonate, suggesting that the sulfate to carbonate ratio may be a determining factor for the competitiveness of the Hap-A and Hap-B phylotypes in natural settings.This work was supported by a grant from the National Science Foundation to Y. Huang (EAR06-02325) and a Brown University Graduate School Dissertation Fellowship to J. L. Toney

The relationship between psychosocial factors and psychological symptoms in menopausal women

修士論文要

Antibody limits in vivo murid herpesvirus-4 replication by IgG Fc receptor-dependent functions

Antibody is an important antiviral defence. However, it is considered to do little against human gamma-herpesviruses, which establish predominantly latent infections regulated by T cells. One limitation on analysing these infections has been that latency is already well-established at clinical presentation; early infection may still be accessible to antibody. Here, using murid herpesvirus-4 (MuHV-4), we tested the impact of adoptively transferred antibody on early gamma-herpesvirus infection. Immune sera and neutralizing and non-neutralizing monoclonal antibodies (mAbs) all reduced acute lytic MuHV-4 replication. The reductions, even by neutralizing mAbs, were largely or completely dependent on host IgG Fc receptors. Therefore, passive antibody can blunt acute gamma-herpesvirus lytic infection, and does this principally by IgG Fc-dependent functions rather than by neutralization

The association of RANTES polymorphism with severe acute respiratory syndrome in Hong Kong and Beijing Chinese

<p>Abstract</p> <p>Background</p> <p>Chemokines play important roles in inflammation and antiviral action. We examined whether polymorphisms of <it>RANTES, IP-10 </it>and <it>Mig </it>affect the susceptibility to and outcome of severe acute respiratory syndrome (SARS).</p> <p>Methods</p> <p>We tested the polymorphisms of <it>RANTES, IP-10 </it>and <it>Mig </it>for their associations with SARS in 495 Hong Kong Chinese SARS patients and 578 controls. Then we tried to confirm the results in 356 Beijing Chinese SARS patients and 367 controls.</p> <p>Results</p> <p><it>RANTES </it>-28 G allele was associated with SARS susceptibility in Hong Kong Chinese (<it>P </it>< 0.0001, OR = 2.80, 95%CI:2.11–3.71). Individuals with <it>RANTES </it>-28 CG and GG genotypes had a 3.28-fold (95%CI:2.32–4.64) and 3.06-fold (95%CI:1.47–6.39) increased risk of developing SARS respectively (<it>P </it>< 0.0001). This -28 G allele conferred risk of death in a gene-dosage dependent manner (<it>P </it>= 0.014) with CG and GG individuals having a 2.12-fold (95% CI: 1.11–4.06) and 4.01-fold (95% CI: 1.30–12.4) increased risk. For the replication of <it>RANTES </it>data in Beijing Chinese, the -28 G allele was not associated with susceptibility to SARS. However, -28 CG (OR = 4.27, 95%CI:1.64–11.1) and GG (OR = 3.34, 95%CI:0.37–30.7) were associated with admission to intensive care units or death due to SARS (<it>P </it>= 0.011).</p> <p>Conclusion</p> <p><it>RANTES </it>-28 G allele plays a role in the pathogenesis of SARS.</p

Τι είναι η πατρίδα μας; [What is our fatherland?]

Ποια είναι η πατρίδα κάποιων; Ας δούμε το θέμα λίγο σαν πρόβλημα της φιλοσοφίας της γλώσσας: για ένα κατηγόρημα «Άγγλος» «Γάλλος», «Πορτογάλος», «Βέλγος», «Φλαμανδός», πώς να αποφασίσουμε ποια αντικείμενα (ποιοι άνθρωποι) εμπίπτουν σε ποιο κατηγόρημα; Έχουν τελικά νόημα αυτά τα κατηγορήματα; Η χρήση και κατάχρηση αυτών των κατηγορημάτων έχει αποτελέσει μια από τις κυριότερες πηγές δυστυχίας στη διάρκεια των δύο τελευταίων αιώνων και συνεχίζει να είναι στην ημερήσια διάταξη. Συνήθως εμφανίζεται στα πλαίσια αγώνων για την απόκτηση «ελευθερίας» σε αντιπαράθεση με την «καταπίεση», και σε παγκόσμιο επίπεδο και κοντύτερα μας: στη Μακεδονία, την Καταλονία, την Ιρλανδία, το Κουρδιστάν ... Νομίζω πώς αρκετά από αυτά βασίζονται σε λάθη

Asthmatics Exhibit Altered Oxylipin Profiles Compared to Healthy Individuals after Subway Air Exposure

Asthma is a chronic inflammatory lung disease that causes significant morbidity and mortality worldwide. Air pollutants such as particulate matter (PM) and oxidants are important factors in causing exacerbations in asthmatics, and the source and composition of pollutants greatly affects pathological implications.This randomized crossover study investigated responses of the respiratory system to Stockholm subway air in asthmatics and healthy individuals. Eicosanoids and other oxylipins were quantified in the distal lung to provide a measure of shifts in lipid mediators in association with exposure to subway air relative to ambient air.Sixty-four oxylipins representing the cyclooxygenase (COX), lipoxygenase (LOX) and cytochrome P450 (CYP) metabolic pathways were screened using liquid chromatography-tandem mass spectrometry (LC-MS/MS) of bronchoalveolar lavage (BAL)-fluid. Validations through immunocytochemistry staining of BAL-cells were performed for 15-LOX-1, COX-1, COX-2 and peroxisome proliferator-activated receptor gamma (PPARγ). Multivariate statistics were employed to interrogate acquired oxylipin and immunocytochemistry data in combination with patient clinical information.Asthmatics and healthy individuals exhibited divergent oxylipin profiles following exposure to ambient and subway air. Significant changes were observed in 8 metabolites of linoleic- and α-linolenic acid synthesized via the 15-LOX pathway, and of the COX product prostaglandin E(2) (PGE(2)). Oxylipin levels were increased in healthy individuals following exposure to subway air, whereas asthmatics evidenced decreases or no change.Several of the altered oxylipins have known or suspected bronchoprotective or anti-inflammatory effects, suggesting a possible reduced anti-inflammatory response in asthmatics following exposure to subway air. These observations may have ramifications for sensitive subpopulations in urban areas

Papers, posters, and keynote presented at the 26th Polar Libraries Colloquy, hosted by the University of Alaska Fairbanks, Fairbanks, Alaska, USA 10 – 15 July 2016

Published July 2023 by the University of Alaska Anchorage, UAA/APU Consortium Library, and edited by Daria O. Carle. Copyright in individual papers is held by the contributors. A digital copy of this publication can be found at https://polarlibraries.org/colloquy-proceedings/ and in ScholarWorks, the University of Alaska’s Institutional Repository, https://scholarworks.alaska.edu/. A copy of the 2016 Colloquy program is also available at https://polarlibraries.org/colloquy-proceedings/. Further information on the Polar Libraries Colloquy, including details of membership and upcoming conferences, is available at https://polarlibraries.orgHistory of Polar Information Science / Working in Antarctica: Mapping a Changing Experience through the British Antarctic Survey / Géoindex+: A Geospatial Platform for Northern Historical and Research Data / Establishing Criteria for the Development of the “Northern Collection” at Université Laval’s Library: An Exploratory Approach / Introducing Two New Reserach Platforms: seaiceportal.de and expedition.awi.de (abstract only) / Establishing a Digital Library Service for the Inuvialuit Settlement Region / Changing Patterns of Polar Research / Mapping the Rescue of an Archive / Byrd 1933: Films from the Discovery Lecture Series / History of the Elmer E. Rasmuson Library and Its Rare Books Collection / A Roadmap to Navigate the Range of Polar Libraries, Databases, and Archives Now Available Online / Mapping Change with Finna in an Arctic Research Joint Library (paper not listed in program) / Mapping Chang in a Small Library Environment: From Reading Room to Communications Center (abstract only) / The Continued Evolution of the Cold Regions Bibliography Project: Current Status of the Antarctic Bibliography and the Antarctic Journal of the United States and its Predecessors / Connect the North: The Arctic Connect Project / Languages and Dialects in the Digital Library North (abstract only) / Bridging Arctic Indigenous Knowledge with the Digital World: Sharing Indigenous Ways of Knowing in Partnership with Arctic Communities (abstract only) / The Canadian Consortium for Arctic Data Interoperability (abstract and poster