Nine years of rockfish surveys in the Strait of Juan de Fuca

For decades, biologists from the Seattle Aquarium have been informally monitoring bottom fish on rocky reefs in Neah Bay, Washington. Based on increasing concern over the long term stability of bottom fish populations in this area by both state and federal agencies, the Aquarium formalized monitoring in 2005 with diver-based video surveys to quantify bottom fish (rockfish and lingcod) diversity and abundance over time. Divers performed 100-meter video transects, devised to be non-invasive and repeatable, to assess diurnally active and relatively sessile bottom fishes over time. Transects were conducted in the Strait of Juan de Fuca each year in August from 2005-2013 at five permanently marked sites. Density and diversity of bottom fish species were determined by biologists counting fish via the archived video. Over the past nine years, there has been no significant difference in diversity and density of adult rockfish among sites or years, but there have been significant young of the year (YOY) rockfish recruitment events in 2006, 2008 and 2010, termed “jackpot recruitment events”. We also found that young of the year rockfish densities in the “jackpot” years were significantly correlated with lower sea surface temperatures. Rockfish recruitment may be generally poor because larval survival and settlement are dependent upon changing conditions such as climate, abundance of predators, oceanic currents, and chance events. Being long-lived allows the adult population to persist through many years of poor reproduction until a good recruitment year occurs, as in 2006, 2008 and 2010.This study may elucidate significant trends in rockfish diversity and abundance, that will influence long term management plans for rockfish conservation

A vertebrate crossveinless 2 homologue modulates BMP activity and neural crest cell migration

Previous work has revealed that proteins that bind to bone morphogenetic proteins (BMPs) and inhibit their signalling have a crucial role in the spatial and temporal regulation of cell differentiation and cell migration by BMPs. We have identified a chick homologue of crossveinless 2, a Drosophila gene that was identified in genetic studies as a promoter of BMP-like signalling. Chick Cv-2 has a conserved structure of five cysteine-rich repeats similar to those found in several BMP antagonists, and a C-terminal Von Willebrand type D domain. Cv-2 is expressed in the chick embryo in a number of tissues at sites at which elevated BMP signalling is required. One such site of expression is premigratory neural crest, in which at trunk levels threshold levels of BMP activity are required to initiate cell migration. We show that, when overexpressed, Cv-2 can weakly antagonise BMP4 activity in Xenopus embryos, but that in other in vitro assays Cv-2 can increase the activity of co-expressed BMP4. Furthermore, we find that increased expression of Cv-2 causes premature onset of trunk neural crest cell migration in the chick embryo, indicative of Cv-2 acting to promote BMP activity at an endogenous site of expression. We therefore propose that BMP signalling is modulated both by antagonists and by Cv-2 that acts to elevate BMP activity

Relative Abundance of Sixgill Sharks (Hexanchus griseus) in Elliott Bay, Seattle, Washington

The Sixgill Shark Research Project is designed to address gaps in the body of scientific knowledge on bluntnose sixgill sharks (Hexanchus griseus) in Puget Sound. This project utilizes three interwoven techniques: (1) genetics research, (2) visual marker tagging, and (3) video analysis. Seattle Aquarium biologists monitor sixgill shark sightings reported by local divers (since 1999) and study their relative abundance in Elliott Bay under the Aquarium’s pier (since 2003). Here we report on our findings of relative abundance. Bluntnose sixgills are a species of conservation concern. Sixgills are listed as “near threatened” on the IUCN Red List. Living mainly at abyssal depths but also in the shallow waters of the Salish Sea, sixgills are thought to be long-lived and slow-growing, and appear to have established movement corridors and home ranges that remain relatively fixed over time. As apex predators they are important members of marine communities; and, owing to their life history characteristics such as a slow rate of maturity and low reproductive rates, are thought to be extremely vulnerable to exploitation. Here we present our findings of relative abundance of sixgill sharks in Elliot Bay during the two time periods that the research was conducted: 2003-2005 and 2008-2015. We present the number of individual sharks seen each night, number of sharks tagged, number of returning tagged sharks, sex ratios, estimated number of sixgills residing in Elliott Bay (using capture-mark-recapture techniques), and seasonal and long-term trends in abundance

Observations on abundance of bluntnose sixgill sharks, Hexanchus griseus, in an urban waterway in the Salish Sea, 2003-2012

The bluntnose sixgill shark, Hexanchus griseus, is a widely distributed but poorly understood large, apex predator. Anecdotal reports of diver-shark encounters in the late 1990’s and early 2000’s in the Pacific Northwest stimulated interest in the normally deep-dwelling shark and the reason for its presence in the shallow waters of the Salish Sea. Analysis of underwater video documenting sharks at the Seattle Aquarium’s sixgill research site on Seattle’s waterfront and mark-recapture techniques were used to identify individual sharks to answer simple questions about abundance and seasonality. Temporal changes in relative abundance in Puget Sound were reported from a controlled study site from 2003-2012. At the Seattle Aquarium study site, 45 sixgills were observed and tagged with modified Floy visual marker tags, along with an estimated 116 observations of untagged sharks. Mark/Recapture statistical model estimates based on video observations ranged from a high of 98 sharks observed in July of 2004 to a low of 0 sharks observed in several research events from 2008-2012. Both analyses found sixgills significantly more abundant in the summer months at the Aquarium’s research station from 2003-2005 than at any other time during the study

Decrease in Sightings of Sixgill Sharks, Hexanchus griseus, in Elliott Bay, Seattle, WA, United States, a Comparison Between 2003–2005 and 2008–2015

The Bluntnose Sixgill Shark, Hexanchus griseus, is a large predatory shark, has a worldwide distribution and is listed as near-threatened by the International Union of Conservation of Nature (IUCN). The Seattle Aquarium collected observations of free-swimming Sixgill Sharks in Elliott Bay, Washington, under the aquarium’s pier in 20 m of water from 2003 to 2005 and again from 2008 to 2015 using the same methodology. Compared to total Sixgill sightings between 2003 and 2005 (273) fewer total Sixgills were sighted at the aquarium’s research station between 2008 and 2015 (33). The reason for the observed decline in sightings in unknown but based on data from other studies on Sixgills in Puget Sound during the same timeperiod the authors hypothesize the decrease may be due to natural variability of juvenile Sixgill recruitment to Elliott Bay

A Unique 100 Meter Underwater Survey Method Documents Changes in Abundance, Richness, and Community Structure of Hawaiʹi Reef Fishes

Hawai′i coral reefs are essential ecosystems providing resources in the form of food and recreation as well as stabilizing nearshore biodiversity. The Seattle Aquarium has exhibited Hawai′i reef fishes and corals since the mid-1980s to educate guests about these critical ecosystems. In 2009, and in collaboration with Hawai′i’s Division of Aquatic Resources (DAR) and Washington State University, the aquarium expanded its conservation work in Hawai′i through annual surveying of eight reefs along the west coast of Hawai′i via SCUBA-based diver operated video (DOV). Five of the sites are in areas partially closed to most fishing while three sites are in areas partially open to most fishing. 100-meter DOV surveys took place a meter above a horizontal or vertical reef, and survey locations were marked with GPS and fixed underwater markings to enable annual surveys to occur in the same locations. Counts of fish species were subsequently made from the archived video. Over the 11 year dataset we documented increased total abundance at all sites and periods of increasing and decreasing species richness. Multivariate analyses comparing fish community structure before (2009-2012) and after an anomalous warm-water event (2013-2019) documented a persistent shift in community structure. This coincides with a documented marine heat wave in Hawai′i and associated coral bleaching events between 2013-2016. These results suggest that our long-term monitoring program captured a phase shift in community structure associated with changing environmental conditions. These persistent shifts may thus indicate hysteresis at relatively short temporal scales, and ongoing monitoring is required to observe whether the systems shift back to the pre-2013 community structure. As coral reef ecosystems face a multitude of stressors from warming waters to marine pollution, long-term monitoring programs are essential to illuminate trends that may inform conservation and management strategies to preserve these imperiled ecosystems

Terrestrial Gamma-Ray Flashes With Accompanying Elves Detected by ASIM

The Atmosphere-Space Interactions Monitor was designed to monitor Terrestrial Gamma-ray Flashes (TGFs) and Transient Luminous Events (TLEs) from space, enabling the study of how these phenomena are related. In this paper, we present observations of 17 TGFs with accompanying Elves. TGFs are short and highly energetic bursts of gamma photons associated with lightning discharges, whereas Elves are TLEs that are observed as concentric rings of ultraviolet (UV) and visible light at ionospheric altitudes, produced by the excitation of N2 molecules when an electromagnetic pulse hits the base of the ionosphere. Elves were identified when optical detections in the UV band could be clearly distinguished from other optical signals from lightning strokes. The TGFs they accompanied had short durations and were associated with particularly high peak current lightning. Lightning sferics associated with these events were detected by the global lightning network GLD360 and the World Wide Lightning Location Network, and they were, with the exception of one event, observed over ocean or coastal regions. It is likely that these events were associated with Energetic In-cloud Pulses. We show that short duration TGFs tend to be associated with higher peak currents than long duration TGFs.publishedVersio

A Machine Learning Technique to Identify Transit Shaped Signals

We describe a new metric that uses machine learning to determine if a periodic signal found in a photometric time series appears to be shaped like the signature of a transiting exoplanet. This metric uses dimensionality reduction and k-nearest neighbors to determine whether a given signal is sufficiently similar to known transits in the same data set. This metric is being used by the Kepler Robovetter to determine which signals should be part of the Q1–Q17 DR24 catalog of planetary candidates. The Kepler Mission reports roughly 20,000 potential transiting signals with each run of its pipeline, yet only a few thousand appear to be sufficiently transit shaped to be part of the catalog. The other signals tend to be variable stars and instrumental noise. With this metric, we are able to remove more than 90% of the non-transiting signals while retaining more than 99% of the known planet candidates. When tested with injected transits, less than 1% are lost. This metric will enable the Kepler mission and future missions looking for transiting planets to rapidly and consistently find the best planetary candidates for follow-up and cataloging

NASA Exploration Mission 2 Mission Design

Exploration Mission 2 (EM-2) will be NASAs first manned flight on the Space Launch System (SLS) and Orion Spacecraft. The mission has been changed from an SLS Block 1B configuration to Block 1. This change has necessitated a reexamination of the flight profile to determine what changes must be made in order to accommodate the reduced launch vehicle performance on the Block 1. Launch availability and orbital debris risk will be traded to find the best flight profile for both SLS and Orion

Masses, radii, and orbits of small Kepler planets : The transition from gaseous to rocky planets

We report on the masses, sizes, and orbits of the planets orbiting 22 Kepler stars. There are 49 planet candidates around these stars, including 42 detected through transits and 7 revealed by precise Doppler measurements of the host stars. Based on an analysis of the Kepler brightness measurements, along with high-resolution imaging and spectroscopy, Doppler spectroscopy, and (for 11 stars) asteroseismology, we establish low false-positive probabilities (FPPs) for all of the transiting planets (41 of 42 have an FPP under 1%), and we constrain their sizes and masses. Most of the transiting planets are smaller than three times the size of Earth. For 16 planets, the Doppler signal was securely detected, providing a direct measurement of the planet's mass. For the other 26 planets we provide either marginal mass measurements or upper limits to their masses and densities; in many cases we can rule out a rocky composition. We identify six planets with densities above 5 g cm-3, suggesting a mostly rocky interior for them. Indeed, the only planets that are compatible with a purely rocky composition are smaller than 2 R ⊕. Larger planets evidently contain a larger fraction of low-density material (H, He, and H2O).Peer reviewedFinal Accepted Versio