Personality regulation of students’ cognitive states during sports and educational activities

The article is devoted to the study of cognitive mental states and personality properties relationship during educational activities. In practical terms, the most important task in the field of educational psychology is to develop methods for updating and regulation of cognitive states. The study of cognitive states carried out in various forms of educational activity: in an ordinary situation (a lecture) and in a tense situation of training (an exam). N = 90, 1-st year students at the Kazan Federal University. During the research used different questionnaires to diagnose the personality traits and the intensity of students’ cognitive states, such as interest, reflection, concentration, mental stress etc. The data analysis included frequency analysis, the polar group method and the MANOVA method. Revealed that in an everyday situation of educational activity (lecture) the most often experienced states are thoughtfulness, interest and concentration, while in a tense situation of an exam - mental stress, doubt and concentration. In everyday situations, a state of thoughtfulness is observed in individuals with high Conscientiousness, and prevails in friendly, open-minded and emotionally stable students with a focus on the task. During the exam students with high activity, extraversion, and focus on the task experience the state of mental stress. Results of this research will find application in educational systems increasing the effectiveness of educational, scientific and creative activities, through the updating of students’ positive cognitive states. Cognitive states stimulate and regulate cognitive activity, performing the function of development of cognitive processes and intellectual abilities. The influence of personality properties on cognitive states is mediated by both the intensity of educational activity and the interaction of the personality traits.The study was carried out with the financial support of the Russian Foundation for Basic Research, project No. 19-013-00325

Acute- and late-phase matrix metalloproteinase (MMP)-9 activity is comparable in female and male rats after peripheral nerve injury.

BACKGROUND:In the peripheral nerve, pro-inflammatory matrix metalloproteinase (MMP)-9 performs essential functions in the acute response to injury. Whether MMP-9 activity contributes to late-phase injury or whether MMP-9 expression or activity after nerve injury is sexually dimorphic remains unknown. METHODS:Patterns of MMP-9 expression, activity and excretion were assessed in a model of painful peripheral neuropathy, sciatic nerve chronic constriction injury (CCI), in female and male rats. Real-time Taqman RT-PCR for MMP-9 and its endogenous inhibitor, tissue inhibitor of metalloproteinase-1 (TIMP-1) of nerve samples over a 2-month time course of CCI was followed by gelatin zymography of crude nerve extracts and purified MMP-9 from the extracts using gelatin Sepharose-beads. MMP excretion was determined using protease activity assay of urine in female and male rats with CCI. RESULTS:The initial upsurge in nerve MMP-9 expression at day 1 post-CCI was superseded more than 100-fold at day 28 post-CCI. The high level of MMP-9 expression in late-phase nerve injury was accompanied by the reduction in TIMP-1 level. The absence of MMP-9 in the normal nerve and the presence of multiple MMP-9 species (the proenzyme, mature enzyme, homodimers, and heterodimers) was observed at day 1 and day 28 post-CCI. The MMP-9 proenzyme and mature enzyme species dominated in the early- and late-phase nerve injury, consistent with the high and low level of TIMP-1 expression, respectively. The elevated nerve MMP-9 levels corresponded to the elevated urinary MMP excretion post-CCI. All of these findings were comparable in female and male rodents. CONCLUSION:The present study offers the first evidence for the excessive, uninhibited proteolytic MMP-9 activity during late-phase painful peripheral neuropathy and suggests that the pattern of MMP-9 expression, activity, and excretion after peripheral nerve injury is universal in both sexes

The alternatively spliced fibronectin CS1 isoform regulates IL-17A levels and mechanical allodynia after peripheral nerve injury.

BackgroundMechanical pain hypersensitivity associated with physical trauma to peripheral nerve depends on T-helper (Th) cells expressing the algesic cytokine, interleukin (IL)-17A. Fibronectin (FN) isoform alternatively spliced within the IIICS region encoding the 25-residue-long connecting segment 1 (CS1) regulates T cell recruitment to the sites of inflammation. Herein, we analyzed the role of CS1-containing FN (FN-CS1) in IL-17A expression and pain after peripheral nerve damage.MethodsMass spectrometry, immunoblotting, and FN-CS1-specific immunofluorescence analyses were employed to examine FN expression after chronic constriction injury (CCI) in rat sciatic nerves. The acute intra-sciatic nerve injection of the synthetic CS1 peptide (a competitive inhibitor of the FN-CS1/α4 integrin binding) was used to elucidate the functional significance of FN-CS1 in mechanical and thermal pain hypersensitivity and IL-17A expression (by quantitative Taqman RT-PCR) after CCI. The CS1 peptide effects were analyzed in cultured primary Schwann cells, the major source of FN-CS1 in CCI nerves.ResultsFollowing CCI, FN expression in sciatic nerve increased with the dominant FN-CS1 deposition in endothelial cells, Schwann cells, and macrophages. Acute CS1 therapy attenuated mechanical allodynia (pain from innocuous stimulation) but not thermal hyperalgesia and reduced the levels of IL-17A expression in the injured nerve. CS1 peptide inhibited the LPS- or starvation-stimulated activation of the stress ERK/MAPK pathway in cultured Schwann cells.ConclusionsAfter physical trauma to the peripheral nerve, FN-CS1 contributes to mechanical pain hypersensitivity by increasing the number of IL-17A-expressing (presumably, Th17) cells. CS1 peptide therapy can be developed for pharmacological control of neuropathic pain

Production and Application of Multicistronic Constructs for Various Human Disease Therapies

The development of multicistronic vectors has opened up new opportunities to address the fundamental issues of molecular and cellular biology related to the need for the simultaneous delivery and joint expression of several genes. To date, the examples of the successful use of multicistronic vectors have been described for the development of new methods of treatment of various human diseases, including cardiovascular, oncological, metabolic, autoimmune, and neurodegenerative disorders. The safety and effectiveness of the joint delivery of therapeutic genes in multicistronic vectors based on the internal ribosome entry site (IRES) and self-cleaving 2A peptides have been shown in both in vitro and in vivo experiments as well as in clinical trials. Co-expression of several genes in one vector has also been used to create animal models of various inherited diseases which are caused by mutations in several genes. Multicistronic vectors provide expression of all mutant genes, which allows the most complete mimicking disease pathogenesis. This review comprehensively discusses multicistronic vectors based on IRES nucleotide sequence and self-cleaving 2A peptides, including its features and possible application for the treatment and modeling of various human diseases

Non-Standard Errors

In statistics, samples are drawn from a population in a data-generating process (DGP). Standard errors measure the uncertainty in estimates of population parameters. In science, evidence is generated to test hypotheses in an evidence-generating process (EGP). We claim that EGP variation across researchers adds uncertainty: Non-standard errors (NSEs). We study NSEs by letting 164 teams test the same hypotheses on the same data. NSEs turn out to be sizable, but smaller for better reproducible or higher rated research. Adding peer-review stages reduces NSEs. We further find that this type of uncertainty is underestimated by participants

Determination of quantum numbers for several excited charmed mesons observed in B- -> D*(+)pi(-) pi(-) decays

A four-body amplitude analysis of the B − → D * + π − π − decay is performed, where fractions and relative phases of the various resonances contributing to the decay are measured. Several quasi-model-independent analyses are performed aimed at searching for the presence of new states and establishing the quantum numbers of previously observed charmed meson resonances. In particular the resonance parameters and quantum numbers are determined for the D 1 ( 2420 ) , D 1 ( 2430 ) , D 0 ( 2550 ) , D ∗ 1 ( 2600 ) , D 2 ( 2740 ) and D ∗ 3 ( 2750 ) states. The mixing between the D 1 ( 2420 ) and D 1 ( 2430 ) resonances is studied and the mixing parameters are measured. The dataset corresponds to an integrated luminosity of 4.7     fb − 1 , collected in proton-proton collisions at center-of-mass energies of 7, 8 and 13 TeV with the LHCb detector

Updated measurement of decay-time-dependent CP asymmetries in D-0 -> K+ K- and D-0 -> pi(+)pi(-) decays

A search for decay-time-dependent charge-parity (CP) asymmetry in D0 \u2192 K+ K 12 and D0 \u2192 \u3c0+ \u3c0 12 decays is performed at the LHCb experiment using proton-proton collision data recorded at a center-of-mass energy of 13 TeV, and corresponding to an integrated luminosity of 5.4 fb^ 121. The D0 mesons are required to originate from semileptonic decays of b hadrons, such that the charge of the muon identifies the flavor of the neutral D meson at production. The asymmetries in the effective decay widths of D0 and anti-D0 mesons are determined to be A_\u393(K+ K 12) = ( 124.3 \ub1 3.6 \ub1 0.5) 7 10^ 124 and A_\u393(\u3c0+ \u3c0 12) = (2.2 \ub1 7.0 \ub1 0.8) 7 10^ 124 , where the uncertainties are statistical and systematic, respectively. The results are consistent with CP symmetry and, when combined with previous LHCb results, yield A_\u393(K+ K 12) = ( 124.4 \ub1 2.3 \ub1 0.6) 7 10^ 124 and A_\u393(\u3c0+ \u3c0 12) = (2.5 \ub1 4.3 \ub1 0.7) 7 10^ 124

Updated measurement of decay-time-dependent CP asymmetries in D-0 -> K+ K- and D-0 -> pi(+)pi(-) decays

A search for decay-time-dependent charge-parity (CP) asymmetry in D-0 -> K+ K- and D-0 -> pi(+)pi(-) eff decays is performed at the LHCb experiment using proton-proton collision data recorded at a center-of-mass energy of 13 TeV, and corresponding to an integrated luminosity of 5.4 fb(-1). The D-0 mesons are required to originate from semileptonic decays of b hadrons, such that the charge of the muon identifies the flavor of the neutral D meson at production. The asymmetries in the effective decay widths of D-0 and (D) over bar (0) mesons are determined to be A(Gamma)(K+ K-) = (-4.3 +/- 3.6 +/- 0.5) x 10(-4) and A(Gamma) (K+ K- ) = (2.2 +/- 7.0 +/- 0.8) x 10(-4), where the uncertainties are statistical and systematic, respectively. The results are consistent with CP symmetry and, when combined with previous LHCb results, yield A(Gamma) (K+ K-) = (-4.4 +/- 2.3 +/- 0.6) x 10(-4) and A(Gamma) (pi(+)pi(-))= (2.5 +/- 4.3 +/- 0.7) x 10(-4)

Ментальная регуляция психических состояний студентов в повседневной и напряженной ситуациях обучения

Introduction. In modern conditions of life, the problem of regulation of mental states and behavior of the personality is of particular relevance. Today Individual style of behavior self-regulation, or self-regulation of functional states consider existing approaches and concepts as a specific type of personality activity. The central place is occupied by the structures of consciousness: semantic, reflective, representative, etc. in the concept of mental states’ regulation developed by the authors. The functional organization of the structures of consciousness is formed in connection with the specifics of complexes consisting of mental structures, mental states and operational means of regulation. Methods. 132 students took part in the study (average age – 19 years, second-year students of the Institute of Psychology and Education of the Kazan (Volga Region) Federal University). We studied the intensity of manifestations of mental states and the effectiveness of their self-regulation on the mental structures in everyday (at lectures) and in stressful (at exams) situations of learning activity. Separately, we studied the severity of each component of the mental structure: semantic structures, reflection, self-attitude in the regulatory process. We studied mental structures using standardized methods and specially developed author\u27s questionnaires. Results. The situational conditionality of the effectiveness of students’ mental states regulation is revealed. In an everyday situation, the key elements of the functional regulatory structure are the reflection of states, the emotional richness of life and the acceptance of people around. In a stressful situation, students\u27 confidence in their abilities, satisfaction with self-realization and a low level of reflection on experience come to the fore. Discussion. The results confirm the concept of mental states’ regulation, denoting the significant role of the consciousness structures (reflective, semantic, self-system) in the regulation of mental states of students. The results are also consistent with the studies of other authors who deal with this problem.Введение. Проблема регуляции психических состояний и поведения субъекта приобретает особую актуальность в современных условиях жизнедеятельности. Существующие на сегодняшний день подходы и концепции рассматривают, в первую очередь, индивидуальный стиль саморегуляции поведения, или же саморегуляцию функциональных состояний, как специфический вид деятельности субъекта. В разрабатываемой авторами концепции ментальной регуляции психических состояний центральное место занимают структуры сознания: смысловые, рефлексивные, репрезентативные и др. Функциональная организация структур сознания формируется в связи со спецификой комплексов, состоящих из ментальных структур (смысловых, рефлексивных и др.), состояний и операциональных средств регуляции. Методы. В исследовании приняли участие 132 студента (средний возраст – 19 лет), учащиеся 2 курса Института психологии и образования Казанского (Приволжского) федерального университета. В повседневных (на лекциях) и в напряженных (на экзаменах) ситуациях учебной деятельности изучались интенсивность проявлений психических состояний и эффективность их саморегуляции со стороны ментальных структур. Ментальные структуры исследовались с помощью стандартизированных методик, а также с применением специально разработанных авторских анкет. Результаты. Выявлена ситуационная обусловленность эффективности ментальной регуляции психических состояний студентов. В повседневной ситуации ключевыми элементами функциональной регуляторной структуры выступают: рефлексия состояний, эмоциональная насыщенность жизни и принятие окружающими людьми. В свою очередь, в напряженной ситуации на передний план выходят: уверенность студентов в своих силах, удовлетворенность самореализацией и низкий уровень рефлексии прошлого опыта. Обсуждение результатов. Полученные результаты подтверждают основные положения концепции ментальной регуляции психических состояний, обозначая значимую роль структур сознания (рефлексивных, смысловых, системы Я) в регуляции психических состояний студентов. Результаты также согласуются с исследованиями других авторов, занимающихся данной проблемой