127 research outputs found

    New Frontiers of Laser Welding Technology

    Get PDF
    With the advances in power sources and optic technologies, high-power laser welding has been utilized in many applications such as automotive, battery manufacturing, and electronic industries. The low-heat input of laser power and its precise control enables minimal thermal damage and geometric inaccuracy in the weldment. Recently, laser welding has evolved in combination with machine learning, monitoring and control technology, new materials, and new processes. This Special Issue aims to present the recent advances in the development in innovative laser welding technologies based on new laser power sources, laser optics, systems, and monitoring technologies. A total of six papers are presented in this Special Issue

    Gas Metal Arc Welding with Undermatched Filler Wire for hot-press-formed steel of 2.0 GPa strength: Influence of filler wire strength and bead geometry

    Get PDF
    Commercial welding filler wires have less strength than hot-press-forming (HPF) steels. As the 2.0 GPa-HPF steel sheets have been released, their lap welding characteristics were investigated using gas metal arc welding in this study. The base metal was 1.1 mm-thick 2.0 GPa-HPF steel sheets, and three filler metal wires considered in this study (W540, W920, and W980) had tensile strengths of 540, 920, and 980 MPa, respectively. Gas metal arc welding was performed under a controlled short-circuit mode, and the wire feed speed (WFS) was selected as a process parameter. Tensile-shear test and microscopy were performed to evaluate the joint strength and metallurgical characteristics. The joint strength increased when WFS increased. When the WFS was 6 m/min or higher and high strength filler wires were applied to it, a heat affected zone (HAZ) fracture was observed in the tensile-shear test, with a tensile strength of approximately 1150 MPa. The fracture location was the boundary of the sub-critical HAZ (comprising tempered martensite) and intercritical HAZ (comprising polygonal ferrite and martensite). The weld metal (WM) hardness for W540 welds was 270 HV, and that for W920 and W980 was 414–419 HV, while the joint strength for the WM fracture was proportional to the throat thickness. For low WFS (when the heat input per unit length and welding current were low), high strength filler metals enhanced the joint strength, while high welding currents and deep penetration welding modes were recommended for W540. This study provided the filler wire and bead geometry design for the lap welds of 2.0 GPa HPF steel sheets

    Development of Prediction Method for Dimensional Stability of 3D-Printed Objects

    Get PDF
    Fused deposition modeling (FDM), as one of the additive manufacturing processes, is known for strong layer adhesion suitable for prototypes and end-use items. This study used a multiple regression model and statistical analysis to explore the dimensional accuracy of FDM objects. Factors such as inclination angle, layer thickness, support space, and raster angle were examined. Machine learning models (Gaussian process regression (GPR), support vector machines (SVM), and artificial neural network (ANN)) predicted dimensions using 81 datapoints. The mean squared dimensional error (MSDE) between the measured and designed surface profiles was selected as an output for the dimensional accuracy. Support spacing, layer thickness, and raster angle were determined to be statistically significant, and all factors were confirmed as significant predictors. The coefficients of determination for multiple linear regression, GPR, SVM, and ANN models were 76%, 98%, 93%, and 99%, respectively. The mean absolute errors (MAEs)—errors between the measured and the predicted MSDEs—were 0.020 mm and 0.034 mm, respectively, for GPR and SVM models. The MAEs for ANN models were 0.0055 mm for supporting cases and 2.1468 x 10 -5 mm for non-supporting cases

    Cooperative protein structural dynamics of homodimeric hemoglobin linked to water cluster at subunit interface revealed by time-resolved X-ray solution scattering

    Get PDF
    Homodimeric hemoglobin (HbI) consisting of two subunits is a good model system for investigating the allosteric structural transition as it exhibits cooperativity in ligand binding. In this work, as an effort to extend our previous study on wild-type and F97Y mutant HbI, we investigate structural dynamics of a mutant HbI in solution to examine the role of well-organized interfacial water cluster, which has been known to mediate intersubunit communication in HbI. In the T72V mutant of HbI, the interfacial water cluster in the T state is perturbed due to the lack of Thr72, resulting in two less interfacial water molecules than in wild-type HbI. By performing picosecond time-resolved X-ray solution scattering experiment and kinetic analysis on the T72V mutant, we identify three structurally distinct intermediates (I1, I2, and I3) and show that the kinetics of the T72V mutant are well described by the same kinetic model used for wild-type and F97Y HbI, which involves biphasic kinetics, geminate recombination, and bimolecular CO recombination. The optimized kinetic model shows that the R-T transition and bimolecular CO recombination are faster in the T72V mutant than in the wild type. From structural analysis using species-associated difference scattering curves for the intermediates, we find that the T-like deoxy I3 intermediate in solution has a different structure from deoxy HbI in crystal. In addition, we extract detailed structural parameters of the intermediates such as E-F distance, intersubunit rotation angle, and heme-heme distance. By comparing the structures of protein intermediates in wild-type HbI and the T72V mutant, we reveal how the perturbation in the interfacial water cluster affects the kinetics and structures of reaction intermediates of HbI. © 2016 Author(s)1571sciescopu

    Molecular basis for SMC rod formation and its dissolution upon DNA binding.

    Get PDF
    SMC condensin complexes are central modulators of chromosome superstructure in all branches of life. Their SMC subunits form a long intramolecular coiled coil, which connects a constitutive "hinge" dimerization domain with an ATP-regulated "head" dimerization module. Here, we address the structural arrangement of the long coiled coils in SMC complexes. We unequivocally show that prokaryotic Smc-ScpAB, eukaryotic condensin, and possibly also cohesin form rod-like structures, with their coiled coils being closely juxtaposed and accurately anchored to the hinge. Upon ATP-induced binding of DNA to the hinge, however, Smc switches to a more open configuration. Our data suggest that a long-distance structural transition is transmitted from the Smc head domains to regulate Smc-ScpAB's association with DNA. These findings uncover a conserved architectural theme in SMC complexes, provide a mechanistic basis for Smc's dynamic engagement with chromosomes, and offer a molecular explanation for defects in Cornelia de Lange syndrome

    Melting Domain Size and Recrystallization Dynamics of Ice Revealed by Time-Resolved X-ray Scattering

    Full text link
    The phase transition between water and ice is ubiquitous and one of the most important phenomena in nature. Here, we performed time-resolved x-ray scattering experiments capturing the melting and recrystallization dynamics of ice. The ultrafast heating of ice I is induced by an IR laser pulse and probed with an intense x-ray pulse, which provided us with direct structural information on different length scales. From the wide-angle x-ray scattering (WAXS) patterns, the molten fraction, as well as the corresponding temperature at each delay, were determined. The small-angle x-ray scattering (SAXS) patterns, together with the information extracted from the WAXS analysis, provided the time-dependent change of the size and the number of the liquid domains. The results show partial melting (~13 %) and superheating of ice occurring at around 20 ns. After 100 ns, the average size of the liquid domains grows from about 2.5 nm to 4.5 nm by the coalescence of approximately six adjacent domains. Subsequently, we capture the recrystallization of the liquid domains, which occurs on microsecond timescales due to the cooling by heat dissipation and results to a decrease of the average liquid domain size
    corecore