Genetic diversity, linkage disequilibrium and power of a large grapevine (Vitis vinifera L) diversity panel newly designed for association studies

UMR-AGAP Equipe DAVV (Diversité, adaptation et amélioration de la vigne) ; équipe ID (Intégration de Données)International audienceAbstractBackgroundAs for many crops, new high-quality grapevine varieties requiring less pesticide and adapted to climate change are needed. In perennial species, breeding is a long process which can be speeded up by gaining knowledge about quantitative trait loci linked to agronomic traits variation. However, due to the long juvenile period of these species, establishing numerous highly recombinant populations for high resolution mapping is both costly and time-consuming. Genome wide association studies in germplasm panels is an alternative method of choice, since it allows identifying the main quantitative trait loci with high resolution by exploiting past recombination events between cultivars. Such studies require adequate panel design to represent most of the available genetic and phenotypic diversity. Assessing linkage disequilibrium extent and panel power is also needed to determine the marker density required for association studies.ResultsStarting from the largest grapevine collection worldwide maintained in Vassal (France), we designed a diversity panel of 279 cultivars with limited relatedness, reflecting the low structuration in three genetic pools resulting from different uses (table vs wine) and geographical origin (East vs West), and including the major founders of modern cultivars. With 20 simple sequence repeat markers and five quantitative traits, we showed that our panel adequately captured most of the genetic and phenotypic diversity existing within the entire Vassal collection. To assess linkage disequilibrium extent and panel power, we genotyped single nucleotide polymorphisms: 372 over four genomic regions and 129 distributed over the whole genome. Linkage disequilibrium, measured by correlation corrected for kinship, reached 0.2 for a physical distance between 9 and 458 Kb depending on genetic pool and genomic region, with varying size of linkage disequilibrium blocks. This panel achieved reasonable power to detect associations between traits with high broad-sense heritability (> 0.7) and causal loci with intermediate allelic frequency and strong effect (explaining > 10 % of total variance).ConclusionsOur association panel constitutes a new, highly valuable resource for genetic association studies in grapevine, and deserves dissemination to diverse field and greenhouse trials to gain more insight into the genetic control of many agronomic traits and their interaction with the environment

A sensory, chemical and consumer study of the peppery typicality of French Gamay wines from cool-climate vineyards

International audienceAim : Within the protected designation of origin (PDO) Côtes d'Auvergne, Gamay N wines express unique peppery notes that may reflect high levels of rotundone. We investigated the typicality of these wines by determining their sensory, chemical and consumer profiles. Methods and results: Twenty-one Gamay N wines from the 2013 vintage from four French wine-growing areas were assessed by a trained sensory panel (n = 8). Principal component analysis and hierarchical clustering of olfactory data were used to describe differences among regions and to select four wines for a consumer study (n = 87). Gamay N wines from Auvergne had more intense peppery notes and higher rotundone concentrations, two characteristics that showed a significant positive correlation. The large variability in rotundone among the 12 wines from Auvergne was attributed to ethanol content, which was correlated to the rotundone levels in the wines. Those who appreciate wines with a peppery sensory profile were generally managers and professionals who are willing to pay more for a bottle of wine. Conclusion: There were differences in sensory profile and rotundone concentrations in Gamay N wines from cool-climate vineyards. We also identified the consumption profile of those who appreciate peppery wines. Significance and impact of the study: Our results provide a scientific foundation for Auvergne grape growers to promote the typicality of their wines. This research also identifies the key elements for developing the Côtes d'Auvergne wine range and adapting products to consumer profiles

Wine competition: from between-juries consistency to sensory perception of consumers

Wine is a complex product offering to consumer a multitude of choices. Considering this diversity, wine competitions provide a way of helping consumers in their choices. However, the dramatic increase of wine contests in the last fifteen years has led to an increase in the number of award-winning wines. Is a medal really a guarantee of quality or having a medal is just a matter of chance? Two studies were therefore conducted during 3 years in a French wine competition. The first study assessed the impact of the order in which the wine was assessed, and the variability between juries’ judgements and therefore the chances of winning an award. For this purpose, the same wine was placed on all jury tables at the first and penultimate positions of the tasting series. Wines were rated, using the OIV-UIOE score sheet. Score differences were analyzed between tasting juries. The second study sought confirmation by a consumer panel of the medals awarded during the competition. A hedonic test of both award-winning and non-awarded wines was organised, in order to verify whether the awarded wines were indeed preferred by consumers

Wine competition: from between-juries consistency to sensory perception of consumers

Wine is a complex product offering to consumer a multitude of choices. Considering this diversity, wine competitions provide a way of helping consumers in their choices. However, the dramatic increase of wine contests in the last fifteen years has led to an increase in the number of award-winning wines. Is a medal really a guarantee of quality or having a medal is just a matter of chance? Two studies were therefore conducted during 3 years in a French wine competition. The first study assessed the impact of the order in which the wine was assessed, and the variability between juries’ judgements and therefore the chances of winning an award. For this purpose, the same wine was placed on all jury tables at the first and penultimate positions of the tasting series. Wines were rated, using the OIV-UIOE score sheet. Score differences were analyzed between tasting juries. The second study sought confirmation by a consumer panel of the medals awarded during the competition. A hedonic test of both award-winning and non-awarded wines was organised, in order to verify whether the awarded wines were indeed preferred by consumers

Characterization of Vitis vinifera L. somatic variants exhibiting abnormal flower development patterns

Mutants have proven to be a key resource for functional genomic studies in model annual plant species. In perennial plant species where mutants are difficult to generate and to screen, spontaneous somatic variants represent a unique resource to understand the genetic control of complex developmental patterns. The morphological and histological characterization of six Vitis vinifera L. somatic variants that display four different abnormal phenotypes of flower development are described here. A phenotype of reiterated reproductive meristems (RRM), with both flower and petal reiteration, was observed in a somatic variant of the cultivar Carignan. An abnormal development of reproductive organs was displayed by the unfused carpels (UFC) somatic variant of cv. Bouchalès, while a somatic variant of cv. Mourvèdre named carpel-less (CLS) developed abnormal ovules in the absence of carpels. Finally, three independent somatic variants in cvs Gamay, Morrastel, and Pinot displayed a phenotype of multiple perianth whorls (MPW). Gene expression studies showed that the expression profiles of VvMADS-box 1, 2, and 3 (putative orthologues of Arabidopsis flowering genes AG, SEP, and AGL13), were altered during grapevine flower development in the somatic variants, whereas the corresponding original cultivars displayed similar VvMADS-box gene expression profiles. Phenotypic and molecular characterization of these variants allowed the development of hypotheses on genetic functions that might be altered in most of the variants in light of the current ABCDE flower modelPeer reviewe

REIN : un outil au service de l’éclairage des inégalités en santé

International audienceÀ l’occasion des 20 ans du REIN (Réseau Epidémiologie et Information en Néphrologie), un travail de synthèse sur les apports du registre a été mené. Sur la question des inégalités sociales de santé, les messages clés suivants ont été retenus.Les inégalités sociales de santé existent tout au long du parcours du patient atteint d’une maladie rénale chronique et se traduisent par des inégalités territoriales d’accès au traitement par dialyse au domicile ou autonome, à la greffe qu’elle soit préemptive ou non.Les ISS sont retrouvées chez l’adulte mais aussi dans la population pédiatrique.Le genre féminin semble associé à une disparité d’accès à la greffe rénale

Care trajectory differences in women and men with end-stage renal disease after dialysis initiation.

Few studies investigated sex-related differences in care consumption after dialysis initiation. Therefore, the aim of this study was to compare the care trajectory in the first year after dialysis start between men and women by taking into account the context of dialysis initiation. All patients who started dialysis in France in 2015 were included. Clinical data of patients and context of dialysis initiation were extracted from the Renal Epidemiology and Information Network (REIN) registry. Data on care consumption in the first year after dialysis start came from the French national health data system (SNDS): hospital stays 24h for kidney problems and hospital stays >24h for other problems, and consultations with a general practitioner. Variables were compared between men and women with the χ2 test and Student's or Welch t-test and logistic regression models were used to identify the factors associated with care consumption after dialysis start. The analysis concerned 8,856 patients (36% of women). Men were less likely to have a hospital stays >24h for kidney problems than women (OR = 0.8, 95% CI = [0.7-0.9]) and less general practitioner consultations (OR = 0.8, 95% CI = [0.8-0.9]), in the year after dialysis initiation, after adjustment on patient's characteristics. Moreover, hospital stays for vascular access preparation or maintenance were longer in women than men (median duration: 2 days [0-2] vs. 1 day [0-2], p < 0.001). In conclusion, despite greater comorbidities in men, this study found few differences in post-dialysis care trajectory between men and women

Pre-dialysis care trajectory and post-dialysis survival and transplantation access in patients with end-stage kidney disease

Online ahead of print.International audienceBackground: The pre-dialysis care trajectory impact on post-dialysis outcomes is poorly known. This study assessed survival, access to kidney transplant waiting list and to transplantation after dialysis initiation by taking into account the patients' pre-dialysis care consumption (inpatient and outpatient) and the conditions of dialysis start: initiation context (emergency or planned) and vascular access type (catheter or fistula).Methods: Adults who started dialysis in France in 2015 were included. Clinical data came from the French REIN registry and data on the care trajectory from the French National Health Data system (SNDS). The Cox model was used to assess survival and access to kidney transplantation.Results: We included 8856 patients with a mean age of 68 years. Survival was shorter in patients with emergency or planned dialysis initiation with a catheter compared to patients with planned dialysis with a fistula. The risk of death was lower in patients who were seen by a nephrologist more than once in the 6 months before dialysis than in those who were seen only once. The rate of kidney transplant at 1 year post-dialysis was lower for patients with emergency or planned dialysis initiation with a catheter (respectively, HR = 0.5 [0.4; 0.8] and HR = 0.7 [0.5; 0.9]) compared to patients with planned dialysis start with a fistula. Patients who were seen by a nephrologist more than three times between 0 and 6 months before dialysis start were more likely to access the waiting list 1 and 3 years after dialysis start (respectively, HR = 1.3 [1.1; 1.5] and HR = 1.2 [1.1; 1.4]).Conclusions: Nephrological follow-up in the year before dialysis initiation is associated with better survival and higher probability of access to kidney transplantation. These results emphasize the importance of early patient referral to nephrologists by general practitioners

Free Hierarchical Sorting procedure: towards a better understanding of assessor’s perception ?

International audienceInterviews results highlight that expert and familiar consumer panels use top-down strategies (use of concepts, knowledge) while unfamiliar consumer panel use bottom-up strategies (use of perceptions). So, for familiar consumer panel, the exposure and the familiarity to these wines contributed to the use of similar categorization processes than expert panel.➢ Conducting interviews at the end of sorting tasks provides additional informations on categorization strategies and thus a better understanding of assessor’s perception