Experimental In-Field Transfer and Survival of Escherichia coli from Animal Feces to Romaine Lettuce in Salinas Valley, California.

This randomized controlled trial characterized the transfer of E. coli from animal feces and/or furrow water onto adjacent heads of lettuce during foliar irrigation, and the subsequent survival of bacteria on the adaxial surface of lettuce leaves. Two experiments were conducted in Salinas Valley, California: (1) to quantify the transfer of indicator E. coli from chicken and rabbit fecal deposits placed in furrows to surrounding lettuce heads on raised beds, and (2) to quantify the survival of inoculated E. coli on Romaine lettuce over 10 days. E. coli was recovered from 97% (174/180) of lettuce heads to a maximal distance of 162.56 cm (5.33 ft) from feces. Distance from sprinklers to feces, cumulative foliar irrigation, and lettuce being located downwind of the fecal deposit were positively associated, while distance from fecal deposit to lettuce was negatively associated with E. coli transference. E. coli exhibited decimal reduction times of 2.2 and 2.5 days when applied on the adaxial surface of leaves within a chicken or rabbit fecal slurry, respectively. Foliar irrigation can transfer E. coli from feces located in a furrow onto adjacent heads of lettuce, likely due to the kinetic energy of irrigation droplets impacting the fecal surface and/or impacting furrow water contaminated with feces, with the magnitude of E. coli enumerated per head of lettuce influenced by the distance between lettuce and the fecal deposit, cumulative application of foliar irrigation, wind aspect of lettuce relative to feces, and time since final irrigation. Extending the time period between foliar irrigation and harvest, along with a 152.4 cm (5 ft) no-harvest buffer zone when animal fecal material is present, may substantially reduce the level of bacterial contamination on harvested lettuce

Adaptive constraints for feature tracking

In this paper extensions to an existing tracking algorithm are described. These extensions implement adaptive tracking constraints in the form of regional upper-bound displacements and an adaptive track smoothness constraint. Together, these constraints make the tracking algorithm more flexible than the original algorithm (which used fixed tracking parameters) and provide greater confidence in the tracking results. The result of applying the new algorithm to high-resolution ECMWF reanalysis data is shown as an example of its effectiveness

Stacking order dynamic in the quasi-two-dimensional dichalcogenide 1T-TaS2_2 probed with MeV ultrafast electron diffraction

Transitions between different charge density wave (CDW) states in quasi-two-dimensional materials may be accompanied also by changes in the inter-layer stacking of the CDW. Using MeV ultrafast electron diffraction, the out-of-plane stacking order dynamics in the quasi-two-dimensional dichalcogenide 1T-TaS$_2$ is investigated for the first time. From the intensity of the CDW satellites aligned around the commensurate $l$ = 1/6 characteristic stacking order, it is found out that this phase disappears with a 0.5 ps time constant. Simultaneously, in the same experiment, the emergence of the incommensurate phase, with a slightly slower 2.0 ps time constant, is determined from the intensity of the CDW satellites aligned around the incommensurate $l$ = 1/3 characteristic stacking order. These results might be of relevance in understanding the metallic character of the laser-induced metastable "hidden" state recently discovered in this compound

VHF downline communication system for SLAR data

A real time VHF downlink communication system is described for transmitting side-looking airborne radar (SLAR) data directly from an aircraft to a portable ground/shipboard receiving station. Use of this receiving station aboard the U.S. Coast Guard icebreaker Mackinaw for generating real-time photographic quality radar images is discussed. The system was developed and demonstrated in conjunction with the U.S Coast Guard and NOAA National Weather Service as part of the Project Icewarn all weather ice information system for the Great Lakes Winter Navigation Program

Expert chess memory: Revisiting the chunking hypothesis

After reviewing the relevant theory on chess expertise, this paper re-examines experimentally the finding of Chase and Simon (1973a) that the differences in ability of chess players at different skill levels to copy and to recall positions are attributable to the experts' storage of thousands of chunks (patterned clusters of pieces) in long-term memory. Despite important differences in the experimental apparatus, the data of the present experiments regarding latencies and chess relations between successively placed pieces are highly correlated with those of Chase and Simon. We conclude that the 2-second inter-chunk interval used to define chunk boundaries is robust, and that chunks have psychological reality. We discuss the possible reasons why Masters in our new study used substantially larger chunks than the Master of the 1973 study, and extend the chunking theory to take account of the evidence for large retrieval structures (templates) in long-term memory

All-weather ice information system for Alaskan arctic coastal shipping

A near real-time ice information system designed to aid arctic coast shipping along the Alaskan North Slope is described. The system utilizes a X-band Side Looking Airborne Radar (SLAR) mounted aboard a U.S. Coast Guard HC-130B aircraft. Radar mapping procedures showing the type, areal distribution and concentration of ice cover were developed. In order to guide vessel operational movements, near real-time SLAR image data were transmitted directly from the SLAR aircraft to Barrow, Alaska and the U.S. Coast Guard icebreaker Glacier. In addition, SLAR image data were transmitted in real time to Cleveland, Ohio via the NOAA-GOES Satellite. Radar images developed in Cleveland were subsequently facsimile transmitted to the U.S. Navy's Fleet Weather Facility in Suitland, Maryland for use in ice forecasting and also as a demonstration back to Barrow via the Communications Technology Satellite

The photoionization dynamics of the three structural isomers of dichloroethene

Using tunable vacuum-UV radiation from a synchrotron, the threshold photoelectron spectrum, threshold photoelectron photoion coincidence spectrum and ion breakdown diagram of the 1,1, cis-1,2 and trans-1,2 isomers of C$_2$H$_2$Cl$_2$ have been recorded in the range 9-23 eV. The energies of the peaks in the threshold photoelectron spectrum are in good agreement with outer-valence Greens function caculations. The major difference between the isomers, both predicted and observed experimentally is that the F and G states of C$_2$H$_2$Cl$_2^+$ are approximately degenerate for 1,1 and trans-1,2, but well separated for the cis-1,2 isomer. The ground and low-lying valence states of C$_2$H$_2$Cl$_2^+$ are bound, with higher-lying states dissociating to C$_2$H$_2$Cl$^+$ or C$_2$H$_2^+$. The translational kinetic energy release into C$_2$H$_2$Cl$^+$ + Cl is determined as a function of energy. Isolated-state behaviour for the low-lying electronic states of C$_2$H$_2$Cl$_2^+$ becomes more statistical as the energy increases

Vacuum-UV negative photoion spectroscopy of CF3Cl, CF3Br and CF3I

Using synchrotron radiation negative ions have been detected by mass spectrometry following vacuum-UV photoexcitation of trifluorochloromethane (CF$_3$Cl), trifluorobromomethane (CF$_3$Br) and trifluoroiodomethane (CF$_3$I). The anions F$^-$, X$^-$, F$_2^-$, FX$^-$, CF$^-$, CF$_2^-$ and CF$_3^-$ were observed from all three molecules, where X = Cl, Br or I, and their ion yields recorded in the range 8-35 eV. With the exception of Br$^-$ and I$^-$, the anions observed show a linear dependence of signal with pressure, showing that they arise from unimolecular ion-pair dissociation. Dissociative electron attachment, following photoionization of CF$_3$Br and CF$_3$I as the source of low-energy electrons, is shown to dominate the observed Br$^-$ and I$^-$ signals, respectively. Cross sections for ion-pair formation are put on to an absolute scale by calibrating the signal strengths with those of F$^-$ from both SF$_6$ and CF$_4$. These anion cross sections are normalized to vacuum-UV absorption cross sections, where available, and the resulting quantum yields are reported. Anion appearance energies are used to calculate upper limits to 298 K bond dissociation energies for $D^0$(CF$_3$-X) which are consistent with literature values. We report new data for $D^0$(CF$_2$I$^-$-F) ≤ 2.7 ± 0.2 eV and $\Delta_fH^0_{298}$ (CF$_2$I$^+$) ≤ (598 ± 22) kJ mol$^{-1}$. No ion-pair formation is observed below the ionization energy of the parent molecule for CF$_3$Cl and CF$_3$Br, and only weak signals (in both I$^-$ and F$^-$) are detected for CF$_3$I. These observations suggest neutral photodissociation is the dominant exit channel to Rydberg state photoexcitation at these lower energies

Variability of insulin sensitivity during the first 4 days of critical illness

1-pageSafe, effective tight glycaemic control (TGC) can improve outcomes in critical care patients, but is difficult to achieve consistently. Insulin sensitivity defines the metabolic balance between insulin concentration and insulin mediated glucose disposal. Hence, variability of insulin sensitivity can cause variable glycaemia. This study investigates the daily evolution of model-based insulin sensitivity level and variability for critical care patients receiving TGC during the first four days of their ICU stay