Developing nutrition-sensitive value chains in Indonesia

In Indonesia’s eastern Maluku and North Maluku provinces, malnutrition levels are high. Nutrition challenges include monotonous diets with inadequate levels of energy, micronutrients and protein. Studies commissioned by IFAD suggest that strengthened value chains for foods such as bananas, cassava, maize, spinach, sweet potatoes and fish could make business sense for smallholders and lay the foundations for a strong local food system that sustainably delivers nutritious foods for healthy diets. With funding from the German and Canadian governments, IFAD recently carried out a set of studies to determine how to design nutrition-sensitive value chain (NSVC) projects for smallholders. Such projects seek to shape the development of value chains for nutritious commodities in ways that are more likely to address nutrition problems. Investments that increase availability and affordability and promote consumption of nutritious foods, including biofortified or underconsumed varieties; provide extension services as well as nutrition education and behaviour change communications; and improve efficiency of market links and processing could increase incomes of women and men, reduce food losses and increase food safety, while also improving nutritional outcomes, largely through improved diets. Interventions that span multiple value chains can help to diversify and improve the overall quality of the diet

Relationship between plasma fibroblast growth factor-23 concentration and survival time in cats with chronic kidney disease

BACKGROUND: Fibroblast growth factor‐23 (FGF‐23) and parathyroid hormone (PTH) are commonly increased in cats with azotemic chronic kidney disease (CKD). Both are predictors of survival time in human patients, but these relationships have not previously been examined in the cat. OBJECTIVES: To investigate the relationship between plasma FGF‐23 and PTH concentrations at diagnosis of CKD in cats with survival time and with disease progression over 12 months. ANIMALS: 214 azotemic, client‐owned cats (≥9 years). METHODS: Retrospective study: Biochemical and urinary variables at diagnosis of azotemic CKD, including plasma FGF‐23 and PTH concentrations were assessed as predictors of survival time (all‐cause mortality) using Cox regression, and as predictors of CKD progression over 12 months using logistic regression. RESULTS: In the final multivariable Cox regression model, survival was negatively associated with plasma creatinine (P = .002) and FGF‐23 concentrations (P = .014), urine protein‐to‐creatinine ratio (P < .001) and age (P < .001). Survival was positively associated with PCV (P = .004). In the final multivariable logistic regression model, independent predictors of CKD progression included logFGF‐23 and age. Neither plasma phosphate nor PTH was found to be an independent predictor of survival time or of CKD progression. CONCLUSIONS AND CLINICAL IMPORTANCE: Plasma FGF‐23 concentration is a novel prognostic indicator in cats with CKD, independent of other factors including plasma creatinine and phosphate concentrations. Further work is required to assess if FGF‐23 contributes directly to CKD progression, but regardless these findings may make FGF‐23 a useful biomarker for predicting poorer outcomes in cats with CKD

Autophagy activation and enhanced mitophagy characterize the Purkinje cells of pcd mice prior to neuronal death

Purkinje cells are a class of specialized neurons in the cerebellum, and are among the most metabolically active of all neurons, as they receive immense synaptic stimulation, and provide the only efferent output from the cerebellum. Degeneration of Purkinje cells is a common feature of inherited ataxias in humans and mice. To understand Purkinje neuron degeneration, investigators have turned to naturally occurring Purkinje cell degeneration phenotypes in mice to identify key regulatory proteins and cellular pathways. The Purkinje cell degeneration (pcd) mouse is a recessive mutant characterized by complete and dramatic post-natal, cell autonomous Purkinje neuron degeneration and death. As the basis of Purkinje cell death in pcd is unresolved, and contradictory data has emerged for the role of autophagy in Purkinje cell degeneration, we studied the mechanism of Purkinje cell death in pcd mice. BAX null status did not suppress Purkinje neuron death in pcd mice, indicating that classic apoptosis is not responsible for Purkinje cell loss. Interestingly, LC3 Western blot analysis and GFP-LC3 immunostaining of degenerating pcd cerebellum revealed activation of the autophagy pathway. Ultrastructural studies confirmed increased autophagy pathway activity in Purkinje cells, and yielded evidence for mitophagy, in agreement with LC3 immunoblotting of cerebellar fractions. As p62 levels were decreased in pcd cerebellum, our findings suggest that pcd Purkinje cell neurons can execute effective autophagy. However, our results support a role for dysregulated autophagy activation in pcd, and suggest that increased or aberrant mitophagy contributes to the Purkinje cell degeneration in pcd mice

Biopsy-proven adenoviral diarrhea responding to low-dose cidofovir

We present a case of diarrhea secondary to biopsy-proven adenovirus (ADV) infection after autologous peripheral hematopoietic stem cell transplant for multiple myeloma. The patient had a negative plasma polymerase chain reaction for ADV and a dramatic clinical response to low-dose cidofovir. To our knowledge, this is the first report in an adult hematopoietic stem cell recipient of the use of low-dose cidofovir to treat proven ADV gastrointestinal infection.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73090/1/j.1399-3062.2008.00303.x.pd

Developing nutrition-sensitive value chains in Nigeria

With funding from the German and Canadian governments, IFAD recently carried out a set of studies in Nigeria and Indonesia to determine how to design nutrition-sensitive value chain (NSVC) projects for smallholders. Such projects seek to shape the development of value chains for nutritious commodities in ways that are likely to address nutrition problems. In Nigeria, the studies were undertaken in the northern states of Katsina and Sokoto, where the IFAD-funded Climate Change Adaptation and Agribusiness Support Programme (CASP) is being implemented by the Federal Ministry of Agriculture and Rural Development of Nigeria. The studies showed that cowpea, groundnut, soybean, millet and sorghum could contribute to improving nutrition as well as livelihoods for smallholders. The studies revealed that the main nutrition problems for smallholders in the project areas include diets with inadequate energy, micronutrient and protein consumption. Such diets are known to contribute to high levels of wasting and stunting in children and undernutrition in women. Problems associated with generally poor diets are compounded by seasonal fluctuations. Promotion of the production and consumption of the five crops identified above could help improve diets and lay the foundations for a more nutritious local food system that also promotes women’s empowerment and resilience in the face of climate change. Importantly, these crops also make business sense for smallholders and value chain development

P16-45. High avidity CD4+ T cell response directed to an immunodominant Gag epitope in HIV controllers: an ANRS EP36 study

International audiencen.

Spectral optical monitoring of 3C390.3 in 1995-2007: I. Light curves and flux variation of the continuum and broad lines

Here we present the results of the long-term (1995-2007) spectral monitoring of the broad line radio galaxy \object{3C~390.3}, a well known AGN with the double peaked broad emission lines, usually assumed to be emitted from an accretion disk. To explore dimensions and structure of the BLR, we analyze the light curves of the broad H$\alpha$ and H$\beta$ line fluxes and the continuum flux. In order to find changes in the BLR, we analyze the H$\alpha$ and H$\beta$ line profiles, as well as the change in the line profiles during the monitoring period. First we try to find a periodicity in the continuum and H$\beta$ light curves, finding that there is a good chance for quasi-periodical oscillations. Using the line shapes and their characteristics (as e.g. peaks separation and their intensity ratio, or FWHM) of broad H$\beta$ and H$\alpha$ lines, we discuss the structure of the BLR. Also, we cross-correlate the continuum flux with H$\beta$ and H$\alpha$ lines to find dimensions of the BLR. We found that during the monitoring period the broad emission component of the H$\alpha$ and H$\beta$ lines, and the continuum flux varied by a factor of $\approx$4-5. Also, we detected different structure in the line profiles of H$\alpha$ and H$\beta$. It seems that an additional central component is present and superposed to the disk emission. In the period of high activity (after 2002), H$\beta$ became broader than H$\alpha$ and red wing of H$\beta$ was higher than the one of H$\alpha$. We found time lags of $\sim$95 days between the continuum and H$\beta$ flux, and about 120 days between the continuum and H$\alpha$ flux. Variation in the line profiles, as well as correlation between the line and continuum flux during the monitoring period is in the favor of the disk origin of the broad lines with the possible contribution of some additional region and/or some kind of perturbation in the disk.Comment: 32 pages, accepted to A&A, typos correcte

The protein kinase R modifies gut physiology to limit colitis

Here we investigate the function of the innate immune molecule protein kinase R (PKR) in intestinal inflammation. To model a colitogenic role of PKR, we determine the physiological response to dextran sulfate sodium (DSS) of wild-type and two transgenic mice strains mutated to express either a kinase-dead PKR or to ablate expression of the kinase. These experiments recognize kinase-dependent and -independent protection from DSS-induced weight loss and inflammation, against a kinase-dependent increase in the susceptibility to DSS-induced injury. We propose these effects arise through PKR-dependent alteration of gut physiology, evidenced as altered goblet cell function and changes to the gut microbiota at homeostasis that suppresses inflammasome activity by controlling autophagy. These findings establish that PKR functions as both a protein kinase and a signaling molecule in instituting immune homeostasis in the gut

Mitochondrial proteomic profiling reveals increased carbonic anhydrase II in aging and neurodegeneration

Carbonic anhydrase inhibitors are used to treat glaucoma and cancers. Carbonic anhydrases perform a crucial role in the conversion of carbon dioxide and water into bicarbonate and protons. However, there is little information about carbonic anhydrase isoforms during the process of ageing. Mitochondrial dysfunction is implicit in ageing brain and muscle. We have interrogated isolated mitochondrial fractions from young adult and middle aged mouse brain and skeletal muscle. We find an increase of tissue specific carbonic anhydrases in mitochondria from middle-aged brain and skeletal muscle. Mitochondrial carbonic anhydrase II was measured in the Purkinje cell degeneration (pcd5J) mouse model. In pcd5J we find mitochondrial carbonic anhydrase II is also elevated in brain from young adults undergoing a process of neurodegeneration. We show C.elegans exposed to carbonic anhydrase II have a dose related shorter lifespan suggesting that high CAII levels are in themselves life limiting. We show for the first time that the mitochondrial content of brain and skeletal tissue are exposed to significantly higher levels of active carbonic anhydrases as early as in middle-age. Carbonic anhydrases associated with mitochondria could be targeted to specifically modulate age related impairments and disease