Qualidade de morangos submetidos à radiação UV-C pré-colheita

The objective of this work was to evaluate the influence of the use of UV-C radiation at an intensity of 0.53 KJ m-2, during strawberry (Fragaria x ananassa) cultivation, on the physicochemical and phytochemical parameters of the fruits. The used experimental design was completely randomized, in a single-factor arrangement with 11, 19, 29, and 39 UV-C applications and without UV-C application. The hue of the fruits was smaller at the end of the study period. pH varied in strawberries that received 39 applications of UV-C, and the soluble solids content differed with 19 applications. Antioxidant activity was lower in fruits with 11, 19, and 29 UV-C applications, whereas vitamin C content was lower only in fruits that received 29 applications. The use of UV-C radiation at an intensity of 0.53 KJ m-2, during strawberry cultivation, affects the physicochemical and phytochemical parameters of the fruits, but does not cause losses in their quality.O objetivo deste trabalho foi avaliar a influência do uso de radiação UV-C na intensidade de 0,53 KJ m-2, durante o cultivo de morango (Fragaria x ananassa), sobre os parâmetros físico-químicos e fitoquímicos dos frutos. O delineamento experimental utilizado foi inteiramente casualizado, em arranjo unifatorial, com 11, 19, 29 e 39 aplicações de UV-C e sem aplicação de UV-C. A tonalidade dos frutos apresentou menor ângulo ao final do período de estudo. O pH variou em morangos que receberam 39 aplicações de UV-C, e o conteúdo de sólidos solúveis diferiu com 19 aplicações. A atividade antioxidante foi menor nas frutas com 11, 19 e 29 aplicações de UV-C, enquanto o teor de vitamina C foi menor apenas nas frutas que receberam 29 aplicações. O uso de radiação UV-C de 0,53 KJ m-2, durante o cultivo de morango, afeta os parâmetros físico-químicos e fitoquímicos dos frutos, mas não leva à perda de sua qualidade

Clonal chromosomal mosaicism and loss of chromosome Y in elderly men increase vulnerability for SARS-CoV-2

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, we found 133 cases (1.42%) with detectable clonal mosaicism for chromosome alterations (mCA) and 226 males (5.08%) with acquired loss of chromosome Y (LOY). Individuals with clonal mosaic events (mCA and/or LOY) showed a 54% increase in the risk of COVID-19 lethality. LOY is associated with transcriptomic biomarkers of immune dysfunction, pro-coagulation activity and cardiovascular risk. Interferon-induced genes involved in the initial immune response to SARS-CoV-2 are also down-regulated in LOY. Thus, mCA and LOY underlie at least part of the sex-biased severity and mortality of COVID-19 in aging patients. Given its potential therapeutic and prognostic relevance, evaluation of clonal mosaicism should be implemented as biomarker of COVID-19 severity in elderly people. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, individuals with clonal mosaic events (clonal mosaicism for chromosome alterations and/or loss of chromosome Y) showed an increased risk of COVID-19 lethality

The Genome of Anopheles darlingi, the main neotropical malaria vector

Anopheles darlingi is the principal neotropical malaria vector, responsible for more than a million cases of malaria per year on the American continent. Anopheles darlingi diverged from the African and Asian malaria vectors ∼100 million years ago (mya) and successfully adapted to the New World environment. Here we present an annotated reference A. darlingi genome, sequenced from a wild population of males and females collected in the Brazilian Amazon. A total of 10 481 predicted protein-coding genes were annotated, 72% of which have their closest counterpart in Anopheles gambiae and 21% have highest similarity with other mosquito species. In spite of a long period of divergent evolution, conserved gene synteny was observed between A. darlingi and A. gambiae. More than 10 million single nucleotide polymorphisms and short indels with potential use as genetic markers were identified. Transposable elements correspond to 2.3% of the A. darlingi genome. Genes associated with hematophagy, immunity and insecticide resistance, directly involved in vectorhuman and vectorparasite interactions, were identified and discussed. This study represents the first effort to sequence the genome of a neotropical malaria vector, and opens a new window through which we can contemplate the evolutionary history of anopheline mosquitoes. It also provides valuable information that may lead to novel strategies to reduce malaria transmission on the South American continent. The A. darlingi genome is accessible at www.labinfo.lncc.br/index.php/anopheles- darlingi. © 2013 The Author(s)

Avaliação da eficácia de diferentes modalidades de preparo interoperatório do colón associado ou não ao emprego da antibioticoprofilaxia: estudo experimental em ratos Evaluation of the efficacy of different kinds of transoperatory colon washout associating or not the prophylacties with antibiotics: experimental study in rats

A limpeza mecânica do cólon e a antibioticoprolaxia profilática foram os procedimentos responsáveis pela significante diminuição de morbidade e mortalidade na cirurgia colorretal. O objetivo do presente estudo foi avaliar, do ponto de vista bacteriológico, a eficácia de alguns métodos de preparo mecânico do cólon associado ou não ao emprego da antibioticoprolaxia. Foram estudados 64 ratos, machos, Wistar, com peso corporal entre 250-350g. Os animais foram divididos em grupos e submetidos a limpeza transoperatória do cólon utilizando-se diferentes tipos de solução (água de torneira, solução salina isotônica (SSI) e solução de polivinilpirrolidona (PVPI) a 5%). Cada grupo foi subdividido em dois subgrupos, que receberam ou não antibioticoprolaxia (cefoxitina sódica, 30 mg/ Kg, via intraperitoneal) 1 hora antes da intervenção cirúrgica. Os resultados foram comparados com o grupo controle no qual não foi realizado qualquer tipo de procedimento. Não se verificou diferença significativa entre os diferentes tipos de preparo mecânico do cólon. Concluímos que houve uma diminuição da flora bacteriana do cólon com o preparo mecânico, todavia a diminuição foi mais acentuada quando se fez uso da antibioticoprolaxia.<br>Teh significant decreasing in morbity and mortality rates in colorectal surgery was due to the bowel preparation and the antibiotic prophylacticts. The aim of the present study was to evaluate, in a bacteriologic point of view, the different methods of colon cleasing associated or not with an antimicrobial agent. Sixty four male rats, Wistar, ranging in weight from 250g to 350g were used. The animals were allocated for intraoperative irrigation of the colon using different washout solutions ( tap water, isotonic saline solution (SSI), and 5% solution of povidine iodine). Half of the animals of each group received prophylactic antibiotics ( cefoxitin, 30 mg/Kg, intraperitoneal) one hour before procedure. The results were compared with a control group where neither antibiotics prophylactics or bowel preparation were done. No significantly difference was observed when comparing the many types of intraoperative irrigation of the colon. We conclued that the bacterial concentration in the colon lumen has a significantly decrease with the mechanical preparation, however, the most important reduction was achieved with the use of parenteral antibiotics

EFEITOS DO AGAROL<FONT FACE=Symbol>â</FONT> E DO TRIGLICERIL<FONT FACE=Symbol>â</FONT> SOBRE A CICATRIZAÇÃO DE PELE: ESTUDO EXPERIMENTAL EM RATOS

Desde os mais remotos tempos pesquisam-se incessantemente drogas que acelerem o processo cicatricial normal. No Hospital de Clínicas da UFPr preconiza-se a limpeza de feridas limpas com solução salina de NaCl a 0,9%. Alguns serviços têm utilizado empiricamente o Agarol&reg; e o Trigliceril&reg; na confecção do curativo após este procedimento. Este estudo objetivou avaliar experimentalmente a evolução de feridas cutâneas, em ratos, produzidas por incisão cirúrgica tratadas com solução de NaCl a 0,9%, Agarol&reg; e Trigliceril&reg; . Utilizou-se 24 ratos, divididos em três grupos: C, controle, submetido à troca diária de curativo após limpeza com solução de NaCl a 0,9%; grupo A com aplicação de Agarol&reg; e grupo T com aplicação de Trigliceril&reg;. Verificou-se que no terceiro e décimo quarto dias de pós-operatório o Agarol<FONT FACE="Symbol">â</FONT> e o Trigliceril<FONT FACE="Symbol">â</FONT> não influenciaram o processo cicatricial. No sétimo dia de pós-operatório verificou-se houve aumento da quantidade de tecido de granulação nos grupos tratados com Agarol<FONT FACE="Symbol">â</FONT> e diminuição da neovascularização no grupo Trigliceril<FONT FACE="Symbol">â</FONT> .<br>For centuries, mankind has been incessantly researching with drugs that could accelerate the normal wound healing process. In the Hospital de Clinicas da Universidade Federal do Paraná, it has been recommended that the cleansing of clean wounds with saline solution of 0.9% sodium chloride, followed by a cover of dry dressing. However, some services have empirically used Agarol and Trigliceril in the confection of dressings after cleansing. This study aimed to evaluate the evolution of cutaneous wounds treated with normal saline, Agarol and Trigliceril in rats. A group of twenty-four rats were submitted to three skin circular incisions of 1.2 cm in diameter on their backs which exposed the subcutaneous tissue. The wounds were cleaned daily with normal saline solution. This group was distributed at random within three groups: Group C, control, received a clean and dry dressing after cleansing; groups A and T received dressings with Agarol and Trigliceril respectively. The evaluation of the wounds was done on the 3rd, 7th and 14th postoperative days by histological and macroscopic studies. The results showed that on the 3rd and 14th days, no evident influence of the drugs on the healing wound was noticed. On 7th postoperative day, Agarol demonstrated to increase the amount of granulation tissue, while Trigliceril appears to decrease the neovasculature during the healing process