Measurement of late-night salivary cortisol with an automated immunoassay system

Background: Measurement of late-night salivary cortisol concentrations is increasingly used as a screening test in suspected Cushing's syndrome. Cortisol concentrations are typically extremely low in late-night samples and discordant assay-specific reference ranges have been reported. Therefore, the aim of our study was to assess the analytical performance of the first automated cortisol immunoassay specified for salivary measurements and to establish late-night sampling reference-range data for this test. Methods: Salivary cortisol was measured using the Roche Cobas Cortisol assay (Roche Diagnostics). Five salivary pools in different concentration ranges were used to assess the inter-assay imprecision of this test in a two-centre evaluation protocol including two reagent lots. Linearity was tested by serial dilution. Salivary samples were obtained at 23:00 h from 100 apparently healthy volunteers using a commercially available salivary sampling device (Salivette, Sarstedt). A subset of 20 samples was used for method comparison with isotope dilution liquid chromatography-tandem mass spectrometry. Results: Inter-assay coefficients of variation (n=20) between 11.6% and 40.4% were found for mean cortisol concentrations between 12.9 and 2.6 nmol/L, with an estimated functional sensitivity of approximately 5.0 nmol/L. The test also gave linear results in the lowest concentration range between 1.0 and 8.3 nmol/L. Mean late-night salivary cortisol of 5.0 nmol/L was found for healthy individuals; the absolute range was 1.4-16.7 nmol/L, and the 95th percentile was 8.9 nmol/L. Substantially lower concentrations were found with isotope dilution LC-MS/MS compared to immunoassay results (mean concentrations 1.8 and 4.4 nmol/L, respectively). Conclusions: The automated assay investigated was found to offer acceptable analytical performance in the very low concentration range required for late-night salivary cortisol, despite a very short turnaround time. Using this assay, late-night salivary cortisol concentrations below 8.9 nmol/L are typically found in healthy volunteers

Flow Induced by Upwelling Winds in an Equatorward Facing Bay: Gulf of Arauco, Chile

[1] Shipborne observations of hydrographic and flow velocity profiles were combined with wind velocity measurements to describe the characteristics of the wind-induced flow in an equatorward facing bay of central Chile in South America. The measurements, which were taken from two transects and one anchor station, were made during late austral spring, between 4 and 10 December 2000. Most observations concentrated on Boca Grande, a transect that crossed the deep, northern ( equatorward) entrance to the bay. The other transect crossed the smaller and shallower, westward entrance to the bay, Boca Chica. The anchor station was located inside the bay, close to Boca Chica. The period of observations was characterized by persistent upwelling winds of up to 17 m/s. The direction of the wind showed very little variability, but its magnitude exhibited well-defined diurnal fluctuations. Wind speeds peaked at around sunset (2100 local time), which coincided with the deepest pycnocline location. The shallowest pycnocline depth appeared a couple of hours after sunrise. These pycnocline oscillations had amplitudes of similar to5 m and were believed to propagate poleward along the coast and dissipate at the shallow entrance in Boca Chica. This mechanism could have mixed low-oxygen, nutrient-rich waters into the photic zone and enhanced the primary productivity. Observed flows at the Boca Grande transect consisted of downwind flow over the shallow ends and upwind flow in the deepest part. This wind-induced pattern is believed to cause a pair of counterrotating gyres that allow near-surface divergence and ventilation of upwelled subsurface waters at the southern end of the bay. This is the region where the highest concentrations of chlorophyll are identified in color imagery

Consistency of metabolic responses and appetite sensations under postabsorptive and postprandial conditions

The present study aimed to investigate the reliability of metabolic and subjective appetite responses under fasted conditions and following consumption of a cereal-based breakfast. Twelve healthy, physically active males completed two postabsorption (PA) and two postprandial (PP) trials in a randomised order. In PP trials a cereal based breakfast providing 1859 kJ of energy was consumed. Expired gas samples were used to estimate energy expenditure and fat oxidation and 100 mm visual analogue scales were used to determine appetite sensations at baseline and every 30 min for 120 min. Reliability was assessed using limits of agreement, coefficient of variation (CV), intraclass coefficient of correlation and 95% confidence limits of typical error. The limits of agreement and typical error were 292.0 and 105.5 kJ for total energy expenditure, 9.3 and 3.4 g for total fat oxidation and 22.9 and 8.3 mm for time-averaged AUC for hunger sensations, respectively over the 120 min period in the PP trial. The reliability of energy expenditure and appetite in the 2 h response to a cereal-based breakfast would suggest that an intervention requires a 211 kJ and 16.6 mm difference in total postprandial energy expenditure and time-averaged hunger AUC to be meaningful, fat oxidation would require a 6.7 g difference which may not be sensitive to most meal manipulations

Vertical Distribution of Decapod Larvae in the Entrance of an Equatorward Facing Bay of Central Chile: Implications for Transport

Two short biophysical surveys were carried out in order to assess how the decapod crustacean larvae vertical distribution and circulation patterns in an equatorward facing embayment (Gulf of Arauco, 37° S; 73° W) influenced larval transport into and out of the Gulf. The embayment is located at the upwelling area of south central Chile and features a deep (60 m) and a shallow (25 m) pathway of communication with the adjacent coastal ocean. Profiles of zooplankton, temperature, salinity, dissolved oxygen and current velocity were measured during a 22-h period at the shallow entrance to the gulf. In addition, three zooplankton profiles were carried out at the deep entrance to the gulf on the basis of observed changes in hydrographic structure. At the shallow entrance to the gulf, current velocities showed a clear semidiurnal tidal signal, with stronger ebb than flood at the surface and stronger flood at depth. Decapod larval abundances showed a bimodal distribution through time, with a maximum during night-time ebb, and a smaller, second peak at day-time ebb. Larval transport in and out of the Gulf depended on larval vertical distribution and the timing of higher densities. Export was reduced when larvae deepened during ebbing tides or when larvae remained deeper throughout the day. Export was enhanced when larger numbers of individuals occupied ebbing surface waters. At the deep entrance, two groups of crustacean larvae were found associated with two different water types: a surface high oxygen water and a deeper suboxic equatorial subsurface water. A group of larvae that originates in the shelf area appeared entering the Gulf in the deeper waters of this entrance but was absent from the shallower one. Larval vertical distribution in the two layer circulation at the embayment entrances can enhance or preclude retention within the bay. The motion of these layers was determined remotely by upwelling dynamics and modified by local wind (sea breeze) and tides. Overall, high frequency processes (semidiurnal tides, diurnal fluctuations) superimposed onto others of lower frequency (3-8 d period of wind-driven upwelling), may contribute significantly to variations in the transport of individuals from bays in upwelling areas

Engaging Northern Indigenous Communities in Biophysical Research: Pitfalls and Successful Approaches

Guidelines and best practices to engage Indigenous people in Arctic regions in biophysical research have emerged since the 1990s. Despite these guidelines, mainstream scientists still struggle to create effective working relationships with Indigenous people and engage them in their research. We encountered this issue when we visited three communities on Alaska’s west coast to study impactful weather events and the formation of “slush ice berms,” which can protect towns from storm surges. As we worked to build relationships with residents of the towns, we found the existing guidelines are often helpful for telling us what to do—for example, they emphasize the importance of face-to-face communication—but researchers also need to think about how to do it (skills) and how to be (personal attributes). To demonstrate to Indigenous people that we value and respect their culture, researchers could learn to use language that is understandable and that reflects a collaborative rather than a top-down approach. We should be ready to adjust our schedules and to help the community we are visiting, rather than simply focusing on our own needs. We might look for benefits for the community and ensure residents understand and are satisfied with the research we are doing. Some of the necessary attributes we identified are curiosity, honesty, interpersonal awareness, empathy, flexibility, and openness. Although the skills and attributes presented here are useful to bridge the gap between cultures, we caution that there is no specific formula that can guarantee success.Des lignes directrices et des pratiques exemplaires visant à faire participer les peuples autochtones des régions arctiques à la recherche biophysique sont publiées depuis les années 1990. Malgré ces lignes directrices, les scientifiques généraux ont toujours de la difficulté à créer des relations de travail efficaces avec les peuples autochtones et à les faire participer à leurs recherches. Nous avons fait face à cet enjeu quand nous avons visité trois collectivités de la côte ouest de l’Alaska pour étudier les événements climatiques percutants et la formation de « bermes de bouillie de glace » susceptibles de protéger les localités des ondes de tempête. Quand nous avons essayé de nouer des liens avec les résidents des diverses localités, nous avons constaté que les lignes directrices actuelles sont souvent utiles pour nous indiquer quoi faire (par exemple, elles mettent l’accent sur l’importance des communications face à face), mais les chercheurs doivent aussi penser à comment le faire (les compétences) et à comment se présenter (attributs personnels). Pour prouver aux Autochtones qu’ils valorisent et respectent leur culture, les chercheurs pourraient apprendre à employer du vocabulaire qui est compréhensible et qui tient compte d’une démarche de collaboration au lieu d’une démarche hiérarchique du sommet à la base. Il faudrait que nous soyons prêts à adapter nos emplois du temps et à aider la collectivité que nous visitons au lieu de nous concentrer seulement sur nos propres besoins. Il faudrait aussi considérer les avantages pour la collectivité et nous assurer que les résidents comprennent la recherche que nous faisons et soient satisfaits. Parmi les attributs nécessaires pour ces travaux de recherche, notons la curiosité, l’honnêteté, la sensibilisation aux besoins d’autrui, l’empathie, la flexibilité et l’ouverture d’esprit. Même si les compétences et les attributs dont il est question ici aident à combler l’écart entre les cultures, nous tenons à souligner qu’aucune formule particulière ne garantit le succès

Crossovers and Phase Coherence in Cuprate Superconductors

High temperature superconductivity is a property of doped antiferromagnetic insulators. The electronic structure is inhomogeneous on short length and time scales, and, as the temperature decreases, it evolves via two crossovers, before long range superconducting order is achieved. Except for overdoped materials, pairing and phase coherence occur at different temperatures, and phase fluctuations determine both T$_c$ and the temperature dependence of the superfluid density for a wide range of doping. A mechanism for obtaining a high pairing scale in a short coherence length material with a strong poorly-screened Coulomb interaction is described.Comment: 5 pages, Latex, Revte

Impaired tumor necrosis factor-α secretion by CD4 T cells during respiratory syncytial virus bronchiolitis associated with recurrent wheeze

BACKGROUND: Infants with severe respiratory syncytial virus (RSV) bronchiolitis have an increased risk of recurrent wheezing and asthma. We aimed to evaluate the relationships between regulatory T cell (Treg) percentage and cytokine production of in vitro-stimulated CD4+ T cells during acute bronchiolitis and the development of recurrent wheezing in the first 3 years of life. METHODS: We obtained peripheral blood from 166 infants hospitalized with their first episode of RSV-confirmed bronchiolitis. Granzyme B (GZB) expression, and interleukin-10, interferon-γ, tumor necrosis factor-α (TNF-α), IL-4, and IL-5 production by in vitro anti-CD3/CD28- and anti-CD3/CD46-activated CD4+ T cells, and percentage of peripheral Treg (CD4+CD25 RESULTS: Sixty-seven percent (n = 111) of children had wheezing after their initial RSV infection, with 30% having recurrent wheezing. The percentage of peripheral Treg (CD4+CD25 CONCLUSIONS: We demonstrated lower TNF-α production by in vitro stimulated CD4+ T cells during severe RSV bronchiolitis in children that subsequently developed recurrent wheezing, compared with children with no subsequent wheeze. These findings support the role of CD4+ T cell immunity in the development of subsequent wheezing in these children

Justice and conservation: The need to incorporate recognition

In light of the Aichi target to manage protected areas equitably by 2020, we ask how the conservation sector should define justice. We focus in particular on ‘recognition’, because it is the least well understood aspect of environmental justice, and yet highly relevant to conservation because of its concern with respect for local knowledge and cultures. In order to explore the meaning of recognition in the conservation context, we take four main steps. First, we identify four components of recognition to serve as our analytical framework: subjects of justice, the harms that constitute injustice, the mechanisms that produce injustices, and the responses to alleviate these. Secondly, we apply this framework to explore four traditions of thinking about recognition: Hegelian inter-subjectivity, critical theory, southern decolonial theory, and the capabilities approach. Thirdly, we provide three case studies of conservation conflicts highlighting how different theoretical perspectives are illustrated in the claims and practices of real world conservation struggles. Fourthly, we finish the paper by drawing out some key differences between traditions of thinking, but also important areas of convergence. The convergences provide a basis for concluding that conservation should look beyond a distributive model of justice to incorporate concerns for social recognition, including careful attention to ways to pursue equality of status for local conservation stakeholders. This will require reflection on working practices and looking at forms of intercultural engagement that, for example, respect alternative ways of relating to nature and biodiversity

Factors determining the concentration and chemical composition of particulate matter in the air of selected service facilities

The link between increased morbidity and mortality and increasing concentrations of particulate matter (PM) resulted in great attention being paid to the presence and physicochemical properties of PM in closed rooms, where people spends most of their time. The least recognized group of such indoor environments are small service facilities. The aim of this study was to identify factors which determine the concentration, chemical composition and sources of PM in the air of different service facilities: restaurant kitchen, printing office and beauty salon. The average PM concentration measured in the kitchen was 5-fold (PM4, particle fraction ≥ 4 μm) and 5.3-fold (TSP, total PM) greater than the average concentration of these PM fractions over the same period. During the same measurement period in the printing office and in the beauty salon, the mean PM concentration was 10- and 4-fold (PM4) and 8- and 3-fold (TSP) respectively greater than the mean concentration of these PM fractions in outdoor air. In both facilities the main source of PM macro-components, especially organic carbon, were chemicals, which are normally used in such places - solvents, varnishes, paints, etc. The influence of some metals inflow from the outdoor air into indoor environment of those facilities was also recognized

Evaluation of different recall periods for the US National Cancer Institute’s Patient-Reported Outcomes version of the Common Terminology Criteria for Adverse Events (PRO-CTCAE)

Aims—The U.S. National Cancer Institute recently developed the Patient-Reported Outcomes version of the Common Terminology Criteria for Adverse Events (PRO-CTCAE). PRO-CTCAE is a library of questions for clinical trial participants to self-report symptomatic adverse events (e.g., nausea). The objective of this study is to inform evidence-based selection of a recall period when PRO-CTCAE is included in a trial. We evaluated differences between 1-week, 2-week, 3-week, and 4-week recall periods, using daily reporting as the reference. Methods—English-speaking patients with cancer receiving chemotherapy and/or radiotherapy were enrolled at four U.S. cancer centers and affiliated community clinics. Participants completed 27 PRO-CTCAE items electronically daily for 28 days, and then weekly over 4 weeks, using 1-week, 2-week, 3-week, and 4-week recall periods. For each recall period, mean differences, effect sizes, and intraclass correlation coefficients were calculated to evaluate agreement between the maximum of daily ratings and the corresponding ratings obtained using longer recall periods (e.g., maximum of daily scores over 7 days vs. 1-week recall). Analyses were repeated using the average of daily scores within each recall period rather than the maximum of daily scores. Results—127 subjects completed questionnaires (57% male; median age 57). The median of the 27 mean differences in scores on the PRO-CTCAE 5-point response scale comparing the maximum daily versus the longer recall period (and corresponding effect size), was −0.20 (−0.20) for 1-week recall; −0.36 (−0.31) for 2-week recall; −0.45 (−0.39) for 3-week recall; and −0.47 (−0.40) for 4-week recall. The median intraclass correlation across 27 items between the maximum of daily ratings and the corresponding longer recall ratings for 1-week recall was 0.70 (range: 0.54–0.82); 2-week recall: 0.74 (range: 0.58–0.83); 3-week recall: 0.72 (range: 0.61–0.84); and 4-week recall: 0.72 (range: 0.64–0.86). Similar results were observed for all analyses using the average of daily scores rather than the maximum of daily scores. Conclusions—1-week recall corresponds best to daily reporting. Although intraclass correlations remain stable over time, there are small but progressively larger differences between daily and longer recall periods at 2, 3, and 4 weeks, respectively. The preferred recall period for the PRO-CTCAE is the past 7 days, although investigators may opt for recall periods of 2, 3, or 4 weeks with an understanding that there may be some information loss