POSITION PAPER OF THE CATALAN SOCIETY OF GASTROENTEROLOGY ABOUT HEPATIC ELASTOGRAPHY 2022

After almost 20 years using transient elastography (TE) for the non-invasive diagnosis of liver fibrosis, its use has been extended to population screening, evaluation of steatosis and complications of cirrhosis. For this reason, the "Catalan Society of Digestology" commissioned a group of experts to update the first Document carried out in 2011.The working group (8 doctors and 4 nurses) prepared a panel of questions based on the online survey "Hepatic Elastography in Catalonia 2022" following the PICO structure and the Delphi method.The answers are presented with the level of evidence, the degree of recommendation and the final consensus after being evaluated by 2 external reviewers.TE uses the simplest and most reliable elastographic method to quantify liver fibrosis, assess steatosis, and determine the risk of complications in patients with cirrhosis.Copyright © 2022 Elsevier España, S.L.U. All rights reserved

A novel pathway producing dimethylsulphide in bacteria is widespread in soil environments

The volatile compound dimethylsulphide (DMS) is important in climate regulation, the sulphur cycle and signalling to higher organisms. Microbial catabolism of the marine osmolyte dimethylsulphoniopropionate (DMSP) is thought to be the major biological process generating DMS. Here we report the discovery and characterisation of the first gene for DMSP-independent DMS production in any bacterium. This gene, mddA, encodes a methyltransferase that methylates methanethiol (MeSH) and generates DMS. MddA functions in many taxonomically diverse bacteria including sediment-dwelling pseudomonads, nitrogen-fixing bradyrhizobia and cyanobacteria, and mycobacteria, including the pathogen Mycobacterium tuberculosis. The mddA gene is present in metagenomes from varied environments, being particularly abundant in soil environments, where it is predicted to occur in up to 76% of bacteria. This novel pathway may significantly contribute to global DMS emissions, especially in terrestrial environments, and could represent a shift from the notion that DMSP is the only significant precursor of DMS

Protocol for a randomised controlled trial of risk screening and early intervention comparing child- and family-focused cognitive-behavioural therapy for PTSD in children following accidental injury

Background: Accidental injury represents the most common type of traumatic event to which a child or adolescent may be exposed, with a significant number of these children going on to experience posttraumatic stress disorder (PTSD). However, very little research has examined potential interventions for the treatment of PTSD in these children. The present trial aims to evaluate and compare child- and family-focused versions of a cognitive-behavioural early intervention for PTSD following accidental injury.Methods/Design: The principal clinical question under investigation is the efficacy of an early, trauma-focused cognitive-behavioural intervention for the treatment of PTSD in children following accidental injury. Specifically, we compare the efficacy of two active treatments (child-focused and family-focused CBT) and a waitlist control (no therapy) to determine which is associated with greater reductions in psychological and health-related outcome measures over time. The primary outcome will be a reduction in trauma symptoms on a diagnostic interview in the active treatments compared to the waitlist control and greater reductions in the family-compared to the child-focused condition. In doing so, this project will also trial a method of stepped screening and assessment to determine those children requiring early intervention for PTSD following accidental injury.Discussion: The present trial will be one of the first controlled trials to examine a trauma-focused CBT, early intervention for children experiencing PTSD following accidental injury (as opposed to other types of traumatic events) and the first within a stepped care approach. In addition, it will provide the first evidence comparing the efficacy of child and family-focused interventions for this target group. Given the significant number of children and adolescents exposed to accidental injury, the successful implementation of this protocol has considerable implications. If efficacious, this early intervention will assist in reducing symptoms of traumatic stress as well as preventing chronic disorder and disability in children experiencing acute PTSD following accidental injury

Are you IDDSI ready?

The International Dysphagia Diet Standardisation Initiative (IDDSI) global framework was launched in November 2015. It was subsequently adopted by the Royal College of Speech and Language Therapists and the British Dietetic Association following expert review and professional membership consultation. In the UK, NHS England established an External IDDSI Reference Group comprising of stakeholders including professional, catering, industry and manufacturing representatives. The IDDSI External reference Group have endorsed a UK Aware, Prepare, Adopt model of implementation and all manufacturers and all healthcare settings are anticipated to be fully IDDSI compliant by April 2019. This requires whole system change with a comprehensive training programme. Those providing and delivering food and drink to individuals with swallowing difficulties need to familiarise themselves with food and drink consistencies and their testing methods. Resources are available to assist local healthcare institutions with implementation of the framework at www.rcslt.org / www.bda.org / www.iddsi.org

Digital Health Europe (DHE) Twinning on Severe Asthma—Kick-off Meeting Report

info:eu-repo/semantics/publishedVersio

Mechanochemical basis of protein degradation by a double-ring AAA+ machine

Molecular machines containing double or single AAA+ rings power energy-dependent protein degradation and other critical cellular processes, including disaggregation and remodeling of macromolecular complexes. How the mechanical activities of double-ring and single-ring AAA+ enzymes differ is unknown. Using single-molecule optical trapping, we determine how the double-ring ​ClpA enzyme from Escherichia coli, in complex with the ​ClpP peptidase, mechanically degrades proteins. We demonstrate that ​ClpA unfolds some protein substrates substantially faster than does the single-ring ​ClpX enzyme, which also degrades substrates in collaboration with ​ClpP. We find that ​ClpA is a slower polypeptide translocase and that it moves in physical steps that are smaller and more regular than steps taken by ​ClpX. These direct measurements of protein unfolding and translocation define the core mechanochemical behavior of a double-ring AAA+ machine and provide insight into the degradation of proteins that unfold via metastable intermediates.Howard Hughes Medical InstituteNational Institutes of Health (U.S.) (Grant AI-16892

A ROC analysis-based classification method for landslide susceptibility maps

[EN] A landslide susceptibility map is a crucial tool for landuse spatial planning and management in mountainous areas. An essential issue in such maps is the determination of susceptibility thresholds. To this end, the map is zoned into a limited number of classes. Adopting one classification system or another will not only affect the map's readability and final appearance, but most importantly, it may affect the decision-making tasks required for effective land management. The present study compares and evaluates the reliability of some of the most commonly used classification methods, applied to a susceptibility map produced for the area of La Marina (Alicante, Spain). A new classification method based on ROC analysis is proposed, which extracts all the useful information from the initial dataset (terrain characteristics and landslide inventory) and includes, for the first time, the concept of misclassification costs. This process yields a more objective differentiation of susceptibility levels that relies less on the intrinsic structure of the terrain characteristics. The results reveal a considerable difference between the classification methods used to define the most susceptible zones (in over 20% of the surface) and highlight the need to establish a standard method for producing classified susceptibility maps. The method proposed in the study is particularly notable for its consistency, stability and homogeneity, and may mark the starting point for consensus on a generalisable classification method.Cantarino-Martí, I.; Carrión Carmona, MÁ.; Goerlich-Gisbert, F.; Martínez Ibáñez, V. (2018). A ROC analysis-based classification method for landslide susceptibility maps. Landslides. 1-18. doi:10.1007/s10346-018-1063-4S118Armstrong MP, Xiao N, Bennett DA (2003) Using genetic algorithms to create multicriteria class intervals for choropleth maps. Ann Assoc Am Geogr 93(3):595–623. https://doi.org/10.1111/1467-8306.9303005Atkinson P, Massari R (1998) Generalised linear modelling of susceptibility to landsliding in the central Apennines, Italy. Comput Geosci 24(4):373–385. https://doi.org/10.1016/S0098-3004(97)00117-9Ayalew L, Yamagishi H (2005) The application of GIS-based logistic regression for landslide susceptibility mapping in the Kakuda-Yahiko Mountains, Central Japan. Geomorphology 65(1–2):15–31. https://doi.org/10.1016/j.geomorph.2004.06.010Baeza C, Lantada N, Amorim S (2016) Statistical and spatial analysis of landslide susceptibility maps with different classification systems. Environ Earth Science 75:1318. https://doi.org/10.1007/s12665-016-6124-1Basofi A, Fariza A, Ahsan AS, Kamal IM (2015) A comparison between natural and head/tail breaks in LSI (landslide susceptibility index) classification for landslide susceptibility mapping: a case study in Ponorogo, East Java, Indonesia. 2015 International Conference on Science in Information Technology, pp 337–342Cantarino I (2013) Elaboración y validación de un modelo jerárquico derivado de SIOSE. Revista de Teledetección 39:5–21Carrara A, Crosta GB, Frattini P (2008) Comparing models of debris-flow susceptibility in the alpine environment. Geomorphology 94(3–4):353–378. https://doi.org/10.1016/j.geomorph.2006.10.033Chacón J, Irigaray C, Fernández T, El Hamdouni R (2006) Engineering geology maps: landslides and geographical information systems. Bull Eng Geol Environ 65(4):341–411Chung CJF, Fabbri AG (2003) Validation of spatial prediction models for landslide hazard mapping. Nat Hazards 30:451–472COPUT (1998) Lithology, exploitation of industrial rocks and landslide risk in the Valencian Community. Thematic Mapping Series. Department of Public Works of the Valencian Regional GovernmentDrummond C, Holte RC (2006) Cost curves: an improved method for visualizing classifier performance. Mach Learn 65(1):95–130Duman TY, Can T, Gokceoglu C, Nefeslioglu HA, Sonmez H (2006) Application of logistic regression for landslide susceptibility zoning of Cekmece Area, Istanbul, Turkey. Environ Geol 51(2):241–256. https://doi.org/10.1007/s00254-006-0322-1Evans IS (1977) The selection of class intervals. Transactions of the Institute of British Geographers. Contemp Cartograph 2(1):98–124. https://doi.org/10.2307/622195Fleiss JL, Levin B, Paik MC (2003) Statistical methods for rates and proportions, Book Series: Wiley Series in Probability and Statistics. John Wiley & Sons. Print ISBN: 9780471526292. doi: https://doi.org/10.1002/0471445428Foody GM (2004) Thematic map comparison: evaluating the statistical significance of differences in classification accuracy. Photogramm Eng Remote Sens 70(5):627–633Fotheringham AS, Brunsdon C, Charlton M (2000) Quantitative geography: perspectives on spatial data analysis. SAGE Publications, Thousand Oaks 270 ppFrattini P, Crosta G, Carrara A (2010) Techniques for evaluating the performance of landslide susceptibility models. Eng Geol 111(1–4):62–72. https://doi.org/10.1016/j.enggeo.2009.12.004Geisser S (1998) Comparing two tests used for diagnostic or screening processes. Stat Probability Lett 40:113–119Greiner M, Pfeiffer D, Smith RD (2000) Principles and practical application of the receiver-operating characteristic analysis for diagnostic tests. Prev Vet Med 45:23–41Günther A, Reichenbach P, Malet JP, van den Eeckhaut M, Hervás J, Dashwood C, Guzzetti F (2013) Tier-based approaches for landslide susceptibility assessment in Europe. Landslides 10:529–546. https://doi.org/10.1007/s10346-012-0349-1Günther A, Van Den Eeckhaut M, Malet J-P, Reichenbach P, Hervás J (2014) Climate-physiographically differentiated Pan-European landslide susceptibility assessment using spatial multi-criteria evaluation and transnational landslide information. Geomorphology 224:69–85Gupta RP, Kanungo DP, Arora MK, Sarkar S (2008) Approaches for comparative evaluation of raster GIS-based landslide susceptibility zonation maps. Int J Appl Earth Obs Geoinf 10(3):330–341. https://doi.org/10.1016/j.jag.2008.01.003Guzzetti F, Reichenbach P, Ardizzone F, Cardinali M, Galli M (2006) Estimating the quality of landslide susceptibility models. Geomorphology 81(1–2):166–184. https://doi.org/10.1016/j.geomorph.2006.04.007Hervás J (2017) El inventario de movimientos de ladera de España ALISSA: Metodología y análisis preliminar. In: Alonso E, Corominas J, Hürlimann M (Eds.), Taludes 2017. Proc. IX Simposio Nacional sobre Taludes y Laderas Inestables, Santander, 27–30 June 2017. CIMNE, Barcelona, pp. 629–639Jaedicke C, Van Den Eeckhaut M, Nadim F et al (2014) Identification of landslide hazard and risk ‘hotspots’ in Europe. Bull Eng Geol Environ 73:325. https://doi.org/10.1007/s10064-013-0541-0Jenks GF (1967) The data model concept in statistical mapping. Int Yearbook Cartograph 7:186–190Jiang B (2013) Head/tail breaks: a new classification scheme for data with a heavy-tailed distribution. Prof Geogr 65(3):482–494. https://doi.org/10.1080/00330124.2012.700499Kiang MY (2003) A comparative assessment of classification methods. Decis Support Syst 35(4):441–454. https://doi.org/10.1016/S0167-9236(02)00110-0Landis JR, Koch GG (1977) The measurement of observer agreement for categorical data. Biometrics 33(1):159–174Langping L, Hengxing L, Changbao G, Yongshuang Z, Quanwen L, Yuming W (2017) A modified frequency ratio method for landslide susceptibility assessment. Landslides 14:727–741. https://doi.org/10.1007/s10346-016-0771-xLee S (2007) Comparison of landslide susceptibility maps generated through multiple logistic regression for three test areas in Korea. Earth Surf Process Landforms 32:2133–2148. https://doi.org/10.1002/esp.1517Liu C, Frazier P, Kumar L (2007) Comparative assessment of the measures of thematic classification accuracy. Remote Sens Environ 107(4):606–616. https://doi.org/10.1016/j.rse.2006.10.010López-Ratón M, Rodríguez-Álvarez MX, Cadarso-Suárez C, Gude-Sampedro F (2014) Optimal cutpoints: an R package for selecting optimal cutpoints in diagnostic tests. J Stat Softw 61(8):4Malet JP, Puissant A, Mathieu A, Van Den Eeckhaut M, Fressard M (2013) Integrating spatial multi-criteria evaluation and expert knowledge for country-scale landslide susceptibility analysis: application to France. In: Margottini C, Canuti P, Sassa K (eds) Landslide science and practice. Springer, Berlin. https://doi.org/10.1007/978-3-642-31325-7_40McGee S (2002) Simplifying likelihood ratios. J Gen Intern Med 17:647–650Metz C (1978) Basic principles of ROC analysis. Semin Nucl Med VIII(4):183–198Nadim F, Kjekstad O, Peduzzi P, Herold C, Jaedicke C (2006) Global landslide and avalanche hotspots. Landslides 3:159–173. https://doi.org/10.1007/s10346-006-0036-1Ohlmacher G, Davis J (2003) Using multiple logistic regression and GIS technology to predict landslide hazard in northeast Kansas, USA. Eng Geol 69(3–4):331–343. https://doi.org/10.1016/S0013-7952(03)00069-3Powell RL, Matzke N, de Souza C Jr, Clark M, Numata I, Hess LL, Roberts DA (2004) Sources of error accuracy assessment of thematic land-cover maps in the Brazilian Amazon. Remote Sens Environ 90(2):221–234. https://doi.org/10.1016/j.rse.2003.12.007Saaty T (1980) The analytic hierarchy process. McGraw Hill, New YorkSmits PC, Dellepiane SG, Schowengerdt RA (1999) Quality assessment of image classification algorithms for land-cover mapping: a review and proposal for a cost-based approach. Int J Remote Sens 20:1461–1486Stehman SV, Czaplewski RL (1998) Design and analysis of thematic map accuracy assessment: fundamental principles. Remote Sens Environ 64:331–344Swets JA (1988) Measuring the accuracy of diagnostic systems. Science 240(4857):1285–1293Van Den Eeckhaut M, Hervás J, Jaedicke C, Malet J-P, Montanarella L, Nadim F (2012) Statistical modelling of Europe-wide landslide susceptibility using limited landslide inventory data. Landslides 8:357–369Varnes DJ (1984) Landslide hazard zonation: a review of principles and practice. Natural hazards. UNESCO, ParisZhu X (2016) GIS for environmental applications. Routledge, Abingdon, p 490Zweig MH, Campbell G (1993) Receiver-operating characteristic (ROC) plots: a fundamental evaluation tool in clinical medicine. Clin Chem 39(4):561–57

Are Females More Responsive to Emotional Stimuli? A Neurophysiological Study Across Arousal and Valence Dimensions

Men and women seem to process emotions and react to them differently. Yet, few neurophysiological studies have systematically investigated gender differences in emotional processing. Here, we studied gender differences using Event Related Potentials (ERPs) and Skin Conductance Responses (SCR) recorded from participants who passively viewed emotional pictures selected from the International Affective Picture System (IAPS). The arousal and valence dimension of the stimuli were manipulated orthogonally. The peak amplitude and peak latency of ERP components and SCR were analyzed separately, and the scalp topographies of significant ERP differences were documented. Females responded with enhanced negative components (N100 and N200), in comparison to males, especially to the unpleasant visual stimuli, whereas both genders responded faster to high arousing or unpleasant stimuli. Scalp topographies revealed more pronounced gender differences on central and left hemisphere areas. Our results suggest a difference in the way emotional stimuli are processed by genders: unpleasant and high arousing stimuli evoke greater ERP amplitudes in women relatively to men. It also seems that unpleasant or high arousing stimuli are temporally prioritized during visual processing by both genders

The Role of Turtles as Coral Reef Macroherbivores

Herbivory is widely accepted as a vital function on coral reefs. To date, the majority of studies examining herbivory in coral reef environments have focused on the roles of fishes and/or urchins, with relatively few studies considering the potential role of macroherbivores in reef processes. Here, we introduce evidence that highlights the potential role of marine turtles as herbivores on coral reefs. While conducting experimental habitat manipulations to assess the roles of herbivorous reef fishes we observed green turtles (Chelonia mydas) and hawksbill turtles (Eretmochelys imbricata) showing responses that were remarkably similar to those of herbivorous fishes. Reducing the sediment load of the epilithic algal matrix on a coral reef resulted in a forty-fold increase in grazing by green turtles. Hawksbill turtles were also observed to browse transplanted thalli of the macroalga Sargassum swartzii in a coral reef environment. These responses not only show strong parallels to herbivorous reef fishes, but also highlight that marine turtles actively, and intentionally, remove algae from coral reefs. When considering the size and potential historical abundance of marine turtles we suggest that these potentially valuable herbivores may have been lost from many coral reefs before their true importance was understood

Enzymatic Mechanisms Involved in Evasion of Fungi to the Oxidative Stress: Focus on Scedosporium apiospermum

The airways of patients with cystic fibrosis (CF) are frequently colonized by various filamentous fungi, mainly Aspergillus fumigatus and Scedosporium species. To establish within the respiratory tract and cause an infection, these opportunistic fungi express pathogenic factors allowing adherence to the host tissues, uptake of extracellular iron, or evasion to the host immune response. During the colonization process, inhaled conidia and the subsequent hyphae are exposed to reactive oxygen species (ROS) and reactive nitrogen species (RNS) released by phagocytic cells, which cause in the fungal cells an oxidative stress and a nitrosative stress, respectively. To cope with these constraints, fungal pathogens have developed various mechanisms that protect the fungus against ROS and RNS, including enzymatic antioxidant systems. In this review, we summarize the different works performed on ROS- and RNS-detoxifying enzymes in fungi commonly encountered in the airways of CF patients and highlight their role in pathogenesis of the airway colonization or respiratory infections. The potential of these enzymes as serodiagnostic tools is also emphasized. In addition, taking advantage of the recent availability of the whole genome sequence of S. apiospermum, we identified the various genes encoding ROS- and RNS-detoxifying enzymes, which pave the way for future investigations on the role of these enzymes in pathogenesis of these emerging species since they may constitute new therapeutics targets