Avaliação de soluções energéticas em ambiente hospitalar : uma oportunidade de negócio ESE : Unidade Local de Saúde de Matosinhos, EPE

Estágio realizado na Unidade Local de Saúde de Matosinhos, EPE (Hospital Pedro Hispano), e orientado pelo Eng. Pedro MachadoTese de mestrado integrado. Engenharia Mecânica. Faculdade de Engenharia. Universidade do Porto. 201

Technical Report: Gingival Vein Punction: A New Simple Technique for Drug Administration or Blood Sampling in Rats and Mice

Blood collection or intravenous injection of substances in rats and mice are necessary for a wide variety of scientific studies. To date, several methods have been developed to access different vessels, according to the different research purpose. However, animal behavioural responses like stress, pain or traumatic injury during some procedures may influence subsequent results. In this technical report we demonstrate the advantages of using the labialis mandibularis vein route that, in addition, seems to prevent unnecessary animal suffering

Potassium nitrate priming to mitigate the salt stress on cucumber seedlings

Salinity is one of the abiotic stresses that further limit the seed germination and growth of plants because of low water potential of the soil. In this study, seeds of cucumber (Cucumis sativus L., cv. Aodai Melhorado) were used to investigate the effects of potassium nitrate priming on germination and early seedling growth under different salinity levels. It was hypothesized that priming with potassium nitrate may improve seed germination and plant establishment by mitigating the negative effects of saline stress through its role in cell osmotic balance. The seeds were soaked in distilled water or in a 2 g L–1 KNO3 solution at 25 °C for 6 hours, and after drying, were distributed in plastic boxes with blotter paper containing different salt solutions prepared with concentrations of 0 (control), 25, 50, 75 and 100 mmol NaCl L–1. The plastic boxes were kept into a seed germinator, at 25 °C for 10 days. A completely randomized design in a 2 × 5 factorial scheme with four replications of 25 seeds each was used. The results showed that the priming of cucumber seeds with potassium nitrate had little effect to improve germination capacity and growth rate of seedlings under salt stress conditions. Low salt concentrations may induce osmotic adjustment activity in the cucumber plants and lead to increases in hypocotyl and radicle length of seedlings, whereas higher concentrations cause severe inhibition of plant growth. The ‶Aodai Melhorado″ cucumber cultivar is a moderately tolerant genotype to salt stress during the germination and seedling establishment stage by presenting yield stability index greater than 0.50 until the level of 100 mmol NaCl L–1.Salinity is one of the abiotic stresses that further limit the seed germination and growth of plants because of low water potential of the soil. In this study, seeds of cucumber (Cucumis sativus L., cv. Aodai Melhorado) were used to investigate the effects of potassium nitrate priming on germination and early seedling growth under different salinity levels. It was hypothesized that priming with potassium nitrate may improve seed germination and plant establishment by mitigating the negative effects of saline stress through its role in cell osmotic balance. The seeds were soaked in distilled water or in a 2 g L-1 KNO3 solution at 25 ºC for 6 hours, and after drying, were distributed in plastic boxes with blotter paper containing different salt solutions prepared with concentrations of 0 (control), 25, 50, 75 and 100 mmol NaCl L-1. The plastic boxes were kept into a seed germinator, at 25 ºC for 10 days. A completely randomized design in a 2 x 5 factorial scheme with four replications of 25 seeds each was used. The results showed that the priming of cucumber seeds with potassium nitrate had little effect to improve germination capacity and growth rate of seedlings under salt stress conditions. Low salt concentrations may induce osmotic adjustment activity in the cucumber plants and lead to increases in hypocotyl and radicle length of seedlings, whereas higher concentrations cause severe inhibition of plant growth. The “Aodai Melhorado” cucumber cultivar is a moderately tolerant genotype to salt stress during the germination and seedling establishment stage by presenting yield stability index greater than 0.50 until the level of 100 mmol NaCl L-1

Crescimento de plantas e severidade da mancha foliar em eucalipto a diferentes concentrações de CO2 no ar

O objetivo deste trabalho foi avaliar os efeitos do aumento da concentração de CO2 do ar sobre o crescimento de plantas e sobre a mancha foliar causada por Cylindrocladium candelabrum em Eucalyptus urophylla. As mudas foram cultivadas durante 30 dias, a 451, 645, 904, 1.147 µmol mol-1 de CO2; em seguida, elas foram inoculadas com o patógeno e mantidas nas mesmas condições por sete dias. O aumento da concentração de CO2 aumentou a altura de plantas e a massa de matéria seca da parte aérea, e diminuiu a incidência e a severidade da doença. O diâmetro do caule não foi afetado pelos tratamentos. O aumento das concentrações de CO2 atmosférico afeta favoravelmente o crescimento de plântulas de eucalipto e reduz a severidade da mancha foliar

Identificação dos principais temas relacionados a sistemas de suporte a tomada de decisões logísticas de 2000 a 2013

A logística é uma área essencial para a competitividade de uma empresa, e é composta basicamente por decisões relacionadas a localização, transporte, processamento de pedidos, serviços ao cliente, armazenagem e compras. O presente artigo buscou, através de uma revisão bibliométrica da literatura científica de 2000 a 2013, caracterizar os principais temas relacionados aos sistemas utilizados na tomada de decisões logísticas nas diferentes cadeias de suprimentos. A análise identificou 165 artigos relevantes, que foram divididos em modelos de aplicações e modelos conceituais, e em cinco categorias: ambiental, inventários, riscos, desenho da cadeia, e outros. Observou-se que a categoria mais importante foi a de desenho de cadeias, com 47% dos artigos avaliados. Identificaram-se também os principais autores, periódicos científicos e clusters de palavras-chave nos documentos analisados. Trabalhos futuros dizem respeito a uma análise aprofundada dos clusters identificado

Renal Myxoma in a Feline

Background: Renal neoplasms are a rare condition in felines, with metastatic lymphoma presenting the highest incidence rate. Secondary renal neoplasms are more frequent (88%) than primary renal tumors, with primary mesenquimal renal neoplasms accounting for 22% of the cases and the incidence of benign tumors not exceeding 5%. Myxomas are neoplasms in undifferentiated mesenchymal cells with abundant reproduction of the myxoid matrix, with few few case reports about this type of neoplasm in the veterinary literature. This study reports the first case of renal myxoma in a young feline afflicted by granulomatous nephritis and diagnosed with FIP (Feline Infectious Peritonitis).Case: A 9-month-old, male, mixed breed feline, was admitted into the Veterinary Hospital maintained by the School of Veterinary Medicine and Animal Science, Botucatu, Brazil, presenting symptoms such progressive weight loss and occasional episodes of emesis for one week, progressing to anorexia, hematochezia, vocalization, lack of coordination, spasms, anuria and inability to defecate. A physical examination revealed moderate dehydration, low temperatures (< 33ºC), dyspnea (36 mpm), slight hyperglycemia (187 mg/dL) and distension of the abdomen and of the urinary bladder. The hematological assay and the serum biochemistry assay revealed neutrophilic leukocytosis, thrombocytopenia, anemia and slight uremia. In view of the severity of the neurologic signs and lack of response to the clinical treatment, the owner opted to euthanize the animal. The necropsy revealed diffused jaundice, with kidney injuries observed through the pale coloration of the tissue and irregular surface with multiple off-white small nodules distributed in a marked and diffuse way. The same injury pattern was observed markedly in the lungs and discretely in the spleen and liver. The material collected was fixed in a 10% formalin solution, and processed according to the routine technique (Hematoxylin & Eosin). The remaining samples were submitted to a histochemical analysis with the Alcian Blue and Masson’s Trichrome stains, in addition to an immunohistochemical expression test. The histopathological examination with H&E staining revealed multiple focuses of pyogranulomatous injuries in the kidneys, lungs, spleen, uvea and leptomeninges, corroborating the diagnosis of feline infectious peritonitis (FIP). In addition, there was a neoplastic proliferation of fusiform cells among an abundant myxoid matrix, evident in Alcian Blue staining. Normal renal tissue, such as glomeruli or tubules, were occasionally observed inside the neoplastic proliferation. Masson’s Trichrome technique was used to exclude the possibility of a fibroblastic origin. In addition, immunohistochemistry (IHC) was performed to confirm the diagnosis of myxoma using the Vimentin, pan-cytokeratin and p63 antibodies. Discussion: Kidney tumors are rare in veterinary medicine, but have an incidence rate four to five times greater in cats than in dogs. The veterinary literature describes myxomas located in joints, cardiac valves and ureter, but reports of myxomas afflicting the kidneys are more commons in human medicine. This tumor should be included in the differential diagnosis process for young cats with suspicions of renal neoplasms, however, to differentiate between neoplasms with a myxoid component in benign tumors. This is the first reported case of renal myxoma afflicting a young feline with feline infectious peritonitis (FIP) in the veterinary literature

MACROALGAS MARINHAS EXÓTICAS NA COSTA BRASILEIRA: UMA REVISÃO

The introduction of exotic marine species is a recent concern in Brazil, and seaweeds are the least studied group. The first publications deal with intentional introduction of species for aquaculture. Recent papers focus on putative accidental introductions. In this review we summarize the existing knowledge about the presence of non-indigenous seaweeds on the Brazilian coast. Nevertheless, the labeling of a species as an exotic one can be a complex issue. Kappaphycus alvarezii and K. striatum were the only authorized and well documented intentional introductions in Brazil, aiming to carrageenan production. Caulerpa scalpelliformis, known from a large stretch of the northeast and east Brazilian coast, seems to have spread to Rio de Janeiro state and can be considered as the only invasive species in Brazil so far. Available data indicate that this species is impacting benthic communities on Ilha Grande Bay. In this same region, Laurencia caduciramulosa populations appeared in 2001 and begun to spread. Other introductions are mere suspicions: Anotrichium yagii and Dasya brasiliensis are suspected to be exotic for being conspicuous species not reported previously to well studied areas. Cladophora corallicola, Laurencia venusta, Pedobesia ryukyuensis, Porphyra leucosticta, Porphyra rizzinii and Porphyra suborbiculata, all reported on recent surveys and suspected to be newcomers, should rather be considered as cryptogenic because they may have been overlooked or misidentified on previous surveys.La introducción de especies exóticas marinas es un evento reciente en Brasil, en donde las algas son el grupo menos estudiado. Las primeras publicaciones trataron sobre la introducción intencional de especies para su uso en acuicultura, mientras que las más recientes versan sobre introducciones involuntarias. En este trabajo se revisa el conocimiento actual sobre la presencia de macroalgas no nativas en la costa de Brasil, teniendo en cuenta las dificultades propias al proceso de establecer a una especie como tal. Kappaphycus alvarezii y K. striatum son las únicas especies introducidas oficialmente al Brasil para la producción de carragenanos. Caulerpa scalpelliformis una especie presente a lo largo de la costa noreste del país, parece haber llegado recientemente al estado de Río de Janeiro, en donde se comportaría como una especie invasora, siendo la única que podría ser catalogada como tal en la actualidad. Otra especie que parece haber llegado recientemente a la misma región, es Laurencia caduciramulosa, que fue registrada por primera vez el año 2001. Además de estas especies hay algunas evidencias de que Anotrichium yagii y Dasya brasiliensis, actualmente conspicuas y comunes, pero no reportadas en trabajos anteriores, habrían llegado al Brasil en las últimas décadas. Otros casos son los de Cladophora corallicola, Laurencia venusta, Pedobesia ryukyuensis, Porphyra leucosticta, Porphyra rizzinii y Porphyra suborbiculata que han sido registradas recientemente, pero que no necesariamente serían especies exóticas pues son inconspicuas y de difícil determinación.A preocupação com a introdução de organismos exóticos é recente no país, especialmente no que diz respeito às algas. As primeiras publicações a tratarem de algas exóticas no Brasil tiveram como foco a introdução de espécies de interesse comercial, visando à maricultura, enquanto que publicações mais recentes abordaram introduções involuntárias. Nosso trabalho resume o que se sabe sobre a presença de espécies de macroalgas não-nativas na costa brasileira, enfatizando as dificuldades inerentes ao processo de reconhecer uma espécie como tal.  Kappaphycus alvarezii e K. striatum são as únicas espécies introduzidas oficialmente no país visando a produção de matéria prima para obtenção de carragenanas. Caulerpa scalpelliformis, uma espécie que está presente em larga faixa da costa nordeste do país, parece ter chegado recentemente na Ilha Grande (RJ), onde vem se comportando como espécie invasora, a única que poderia ser catalogada nesta categoria até o momento. Outra espécie que parece ter chegado recentemente na mesma região é Laurencia caduciramulosa, registrada pela primeira vez em 2001. Além destas existem algumas evidências de que Anotrichium yagii e Dasya brasiliensis, atualmente conspícuas e comuns, mas não registradas em trabalhos pretéritos, tenham chegado ao Brasil nas últimas décadas. Casos semelhantes são os de Cladophora corallicola, Laurencia venusta, Pedobesia ryukyuensis, Porphyra leucosticta, Porphyra rizzinii e Porphyra suborbiculata, todas registradas há pouco tempo mas não necessariamente exóticas, pelo fato de serem inconspícuas e de difícil determinação

PHYSICAL CHARACTERIZATION OF FRUITS AND SEEDS Inga laurina (Sw.) Willd. (Fabaceae - Mimosaceae)

The main physical and morphological characteristics of Inga laurina (Sw.) Willd. (Fabaceae –Mimosaceae) fruits and seeds, and correlation estimates between these characteristics were investigate in this study. Mature fruits of I. laurina were collected from matrices trees established in a savannah area located at the municipality of Cassilândia, Mato Grosso do Sul, Brazil. The length, width, and thickness of 100 fruits and 100 seeds were measured. The fresh mass of fruits, pulp and seeds, and seed number per fruit were quantified. Data were analyzed by using descriptive statistics, adjustment of statistical distributions, and Spearman's rank correlation analysis (rS). The I. laurina presents variation in the size and weight of fruits, pulp, and seeds, which can be explored for breeding programs and should be considered in the formation of seed lots that are more homogeneous. The fruits of I. laurina species are composed of 41% of pulp, 39% of bark and 20% of the seed. The greater fruit pulp yield can be optimized with the selection of fruits with greater fresh mass and longer due to the high degree of association between these characteristics and the pulp yield. The fruit length is a good indication in the selection of seeds with greater weight