278 research outputs found
Remodelação da margem gengival nas restauraçÔes de classe V: tĂ©cnica BOPT - caso clĂnico
A pressão social no sentido de obtenção de ideais de beleza, tem condicionado os tratamentos
em medicina dentåria, com uma procura crescente de modificaçÔes estéticas do sorriso.
A convencional linha de preparo horizontal executada em prĂłtese fixa, tem sido desafiada por
novas filosofias que preconizam preparos verticais dinĂąmicos, como a BOPT- Biological
Oriented Preparation Techniqueâ. Nesta tĂ©cnica, a linha de acabamento e o volume protĂ©tico
sĂŁo ajustados, de forma a criar um novo perfil de emergĂȘncia ao qual a gengiva se adapta.
O objetivo deste trabalho eÌ atravĂ©s de um caso clĂnico, explanar a tĂ©cnica de restauração de
classes V segundo os princĂpios BOPT, avaliando a capacidade de remodelação da margem
gengival. à intenção também comparar a resposta periodontal de uma restauração executada
pela técnica convencional e uma restauração de classe V realizadas com a técnica BOPT.
Foi efetuada uma revisão bibliogråfica prévia na Pub-Med com as seguintes palavras-chave:
âBOPTâ, âTeÌcnica de PreparaçaÌo Biologicamente Orientadaâ, âBOPT em restauraçÔes de
classe Vâ, âperfil de emergeÌnciaâ, âlesĂ”es cervicaisâ.Social pressure towards obtaining ideals of beauty has conditioned treatments in dentistry, with
an increasing demand for aesthetic changes in the smile.
The conventional line of horizontal preparation performed in fixed prosthesis, has been
challenged by new philosophies that advocate dynamic vertical preparations, such as the BOPTBiological Oriented Preparation Techniqueâ. In this technique, the finishing line and the
prosthetic volume are adjusted, in order to create a new emergency profile to which the gingiva
adapts.
The objective of this work is, through a clinical case, to explain the technique of restoration of
classes V according to the BOPT principles, evaluating the remodeling capacity of the gingival
margin. It is also intended to compare the periodontal response of a restoration performed by
the conventional technique and a class V restoration performed with the BOPT technique.
A previous bibliographic review was carried out at Pub-Med with the following keywords:
"BOPT", "Biologically Oriented Preparation Technique", "BOPT in class V restorations",
"emergency profile", "cervical lesions"
In vitro Interactions between Streptococcus intermedius and Streptococcus salivarius K12 on a Titanium Cylindrical Surface
Peri-implantitis is a steadily rising disease and is caused by oral bacterial pathogens able to form biofilm on implant surfaces and peri-implant tissues, making antibiotics treatment less effective. The use of commercial probiotics against oral pathogens could serve as an alternative to prevent biofilm formation. Streptococcus intermedius is one of the early colonizers of biofilm formation in dental implants. The aim of this study was to model the interaction between S. intermedius and Streptococcus salivarius strain K12, a probiotic bacterium producing bacteriocins. S. intermedius was co-cultured with S. salivarius K12 in an in vitro model simulating the biofilm formation in a dental implant composed by a titanium cylinder system. Biofilm formation rate was assessed by Real-Time PCR quantification of bacterial count and expression levels of luxS gene, used in response to cell density in the biofilm. Biofilm formation, bacteriocin production, luxS expression patterns were found to be already expressed within the first 12 h. More importantly, S. salivarius K12 was able to counter the biofilm formation in a titanium cylinder under the tested condition. In conclusion, our dental implant model may be useful for exploring probiotic-pathogen interaction to find an alternative to antibiotics for peri-implantitis treatment
ubi F is involved in acid stress tolerance and symbiotic competitiveness in Rhizobium favelukesii LPU83
The acidity of soils signifcantly reduces the productivity of legumes mainly because of the detrimental efects of hydrogen ions on the legume plants, leading to the establishment of an inefcient symbiosis and poor biological nitrogen fxation. We recently reported the analysis of the fully sequenced genome of Rhizobium favelukesii LPU83, an alfalfa-nodulating rhizobium with a remarkable ability to grow, nodulate and compete in acidic conditions. To gain more insight into the genetic mechanisms leading to acid tolerance in R. favelukesii LPU83, we constructed a transposon mutant library and screened for mutants displaying a more acid-sensitive phenotype than the parental strain. We identifed mutant Tn833 carrying a singletransposon insertion within LPU83_2531, an uncharacterized short ORF located immediately upstream from ubiF homolog. This gene encodes a protein with an enzymatic activity involved in the biosynthesis of ubiquinone. As the transposon was inserted near the 3âČ end of LPU83_2531 and these genes are cotranscribed as a part of the same operon, we hypothesized that the phenotype in Tn833 is most likely due to a polar efect on ubiF transcription. We found that a mutant in ubiF was impaired to grow at low pH and other abiotic stresses including 5 mM ascorbate and 0.500 mM Zn2+. Although the ubiF mutant retained the ability to nodulate alfalfa and Phaseolus vulgaris, it was unable to compete with the R. favelukesii LPU83 wild-type strain for nodulation in Medicago sativa and P. vulgaris, suggesting that ubiF is important for competitiveness. Here, we report for the frst time an ubiF homolog being essential for nodulation competitiveness and tolerance to specifc stresses in rhizobia.Fil: Martini, MarĂa Carla. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - La Plata. Instituto de BiotecnologĂa y BiologĂa Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de BiotecnologĂa y BiologĂa Molecular; ArgentinaFil: Vacca, Carolina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - La Plata. Instituto de BiotecnologĂa y BiologĂa Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de BiotecnologĂa y BiologĂa Molecular; ArgentinaFil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - La Plata. Instituto de BiotecnologĂa y BiologĂa Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de BiotecnologĂa y BiologĂa Molecular; ArgentinaFil: Draghi, Walter Omar. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - La Plata. Instituto de BiotecnologĂa y BiologĂa Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de BiotecnologĂa y BiologĂa Molecular; ArgentinaFil: Pistorio, Mariano. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - La Plata. Instituto de BiotecnologĂa y BiologĂa Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de BiotecnologĂa y BiologĂa Molecular; ArgentinaFil: Lozano, Mauricio Javier. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - La Plata. Instituto de BiotecnologĂa y BiologĂa Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de BiotecnologĂa y BiologĂa Molecular; ArgentinaFil: Lagares, Antonio. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - La Plata. Instituto de BiotecnologĂa y BiologĂa Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de BiotecnologĂa y BiologĂa Molecular; ArgentinaFil: del Papa, Maria Florencia. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - La Plata. Instituto de BiotecnologĂa y BiologĂa Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de BiotecnologĂa y BiologĂa Molecular; Argentin
Identification and Characterization of a Novel Plasmid-Encoded Laccase-Like Multicopper Oxidase from Ochrobactrum sp. BF15 Isolated from an On-Farm Bio-Purification System
Research background. In recent decades, laccases (p-diphenol-dioxygen oxidoreductases; EC 1.10.3.2) have attracted the attention of researchers due to their wide range of biotechnological and industrial applications. Laccases can oxidize a variety of organic and inorganic compounds, making them suitable as biocatalysts in biotechnological processes. Even though the most traditionally used laccases in the industry are of fungal origin, bacterial laccases have shown an enormous potential given their ability to act on several substrates and in multiple conditions. The present study aims to characterize a plasmid-encoded laccase-like multicopper oxidase (LMCO) from Ochrobactrum sp. BF15, a bacterial strain previously isolated from polluted soil.
Experimental approach. We used in silico profile hidden Markov models to identify novel laccase-like genes in Ochrobactrum sp. BF15. For laccase characterization, we performed heterologous expression in Escherichia coli, purification and activity measurement on typical laccase substrates.
Results and conclusions. Profile hidden Markov models allowed us to identify a novel LMCO, named Lac80. In silico analysis of Lac80 revealed the presence of three conserved copper oxidase domains characteristic of three-domain laccases. We successfully expressed Lac80 heterologously in E. coli, allowing us to purify the protein for further activity evaluation. Of thirteen typical laccase substrates tested, Lac80 showed lower activity on 2,2\u2019-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), pyrocatechol, pyrogallol and vanillic acid, and higher activity on 2,6-dimethoxyphenol.
Novelty and scientific contribution. Our results show Lac80 as a promising laccase for use in industrial applications. The present work shows the relevance of bacterial laccases and highlights the importance of environmental plasmids as valuable sources of new genes encoding enzymes with potential use in biotechnological processes
Asenapine Effects On Peroxidation and Calcium Movements in HL-1 Cells
Introduction Bipolar patients are at higher risk for cardiovascular morbidity and mortality than their
counterparts in the general population. In a recent in vitro study, Asenapine, a new antipsychotic for the
treatment of mania/mixed mania, was found to keep physiological endothelial function by activation of
eNOS-related NO release and to protect endothelial cells against peroxidation by interference with
mitochondria, apoptosis and cell survival.
Objectives To examine the cardiac protective effects elicited by Asenapine against peroxidation and on the
Ca2+ movements.
Methods In HL-1 that had undergone oxidative stress by 20 min hydrogen peroxide the effects of 30 min
pre-treatment with Asenapine on survival and proliferation will be examined. In Fura-2AM loaded HL-1 we will
next analyze the effects of Asenapine on Ca2+ movements and the related involvement of cAMP/PKA and
PLC pathways, CaMKII, L and T type Ca2+ channels and 5HT1A receptors. The role of 'capacitativeâ Ca2+
entry, plasma-membrane Ca2+ pump inhibitor (PMCA) and Na+/Ca2+ exchanger will be analyzed.
Changes of membrane potential caused by interference with K+ channels will be examined, as well.
Results We expect to find a proliferative and anti-peroxidative effect of Asenapine in HL-1 cells. Asenapine
could also affect Ca2+ movements through cAMP/PKA and PLC-dependent signalling and the involvement
of 5HT1A receptors. The effects of Asenapine could also be related to changes of plasma membrane by
interference with K+ channels and the modulation of PMCA activity and Na+/Ca2+ exchanger.
Conclusions We expect to further confirm the protective effect of Asenapine against peroxidative
injuries.Implications will be discusse
Th17/Treg Imbalance in Murine Cystic Fibrosis Is Linked to Indoleamine 2,3-Dioxygenase Deficiency but Corrected by Kynurenines
n/
Field sampling of soil pore water to evaluate the mobile fraction of trace elements in the Iglesiente area (SW Sardinia, Italy)
Patients with Alzheimerâs disease dementia show partially preserved parietal âhubsâ modeled from resting-state alpha electroencephalographic rhythms
Introduction: Graph theory models a network by its nodes (the fundamental unit by which graphs are formed) and connections. âDegreeâ hubs reflect node centrality (the connection rate), while âconnectorâ hubs are those linked to several clusters of nodes (mainly long-range connections). Methods: Here, we compared hubs modeled from measures of interdependencies of between-electrode resting-state eyes-closed electroencephalography (rsEEG) rhythms in normal elderly (Nold) and Alzheimerâs disease dementia (ADD) participants. At least 5 min of rsEEG was recorded and analyzed. As ADD is considered a ânetwork diseaseâ and is typically associated with abnormal rsEEG delta (<4 Hz) and alpha rhythms (8â12 Hz) over associative posterior areas, we tested the hypothesis of abnormal posterior hubs from measures of interdependencies of rsEEG rhythms from delta to gamma bands (2â40 Hz) using eLORETA bivariate and multivariate-directional techniques in ADD participants versus Nold participants. Three different definitions of âconnectorâ hub were used. Results: Convergent results showed that in both the Nold and ADD groups there were significant parietal âdegreeâ and âconnectorâ hubs derived from alpha rhythms. These hubs had a prominent outward âdirectionalityâ in the two groups, but that âdirectionalityâ was lower in ADD participants than in Nold participants. Discussion: In conclusion, independent methodologies and hub definitions suggest that ADD patients may be characterized by low outward âdirectionalityâ of partially preserved parietal âdegreeâ and âconnectorâ hubs derived from rsEEG alpha rhythms
A review of the main genetic factors influencing the course of COVID-19 in Sardinia: the role of human leukocyte antigen-G
Introduction: A large number of risk and protective factors have been identified during the SARS-CoV-2 pandemic which may influence the outcome of COVID-19. Among these, recent studies have explored the role of HLA-G molecules and their immunomodulatory effects in COVID-19, but there are very few reports exploring the genetic basis of these manifestations. The present study aims to investigate how host genetic factors, including HLA-G gene polymorphisms and sHLA-G, can affect SARS-CoV-2 infection.
Materials and Methods: We compared the immune-genetic and phenotypic characteristics between COVID-19 patients (n = 381) with varying degrees of severity of the disease and 420 healthy controls from Sardinia (Italy).
Results: HLA-G locus analysis showed that the extended haplotype HLA-G*01:01:01:01/UTR-1 was more prevalent in both COVID-19 patients and controls. In particular, this extended haplotype was more common among patients with mild symptoms than those with severe symptoms [22.7% vs 15.7%, OR = 0.634 (95% CI 0.440 â 0.913); P = 0.016]. Furthermore, the most significant HLA-G 3âUTR polymorphism (rs371194629) shows that the HLA-G 3âUTR Del/Del genotype frequency decreases gradually from 27.6% in paucisymptomatic patients to 15.9% in patients with severe symptoms (X2 = 7.095, P = 0.029), reaching the lowest frequency (7.0%) in ICU patients (X2 = 11.257, P = 0.004). However, no significant differences were observed for the soluble HLA-G levels in patients and controls. Finally, we showed that SARS-CoV-2 infection in the Sardinian population is also influenced by other genetic factors such as ÎČ-thalassemia trait (rs11549407C>T in the HBB gene), KIR2DS2/HLA-C C1+ group combination and the HLA-B*58:01, C*07:01, DRB1*03:01 haplotype which exert a protective effect [P = 0.005, P = 0.001 and P = 0.026 respectively]. Conversely, the Neanderthal LZTFL1 gene variant (rs35044562A>G) shows a detrimental consequence on the disease course [P = 0.001]. However, by using a logistic regression model, HLA-G 3âUTR Del/Del genotype was independent from the other significant variables [ORM = 0.4 (95% CI 0.2 â 0.7), PM = 6.5 x 10-4].
Conclusion: Our results reveal novel genetic variants which could potentially serve as biomarkers for disease prognosis and treatment, highlighting the importance of considering genetic factors in the management of COVID-19 patients
Functional cortical source connectivity of resting state electroencephalographic alpha rhythms shows similar abnormalities in patients with mild cognitive impairment due to Alzheimer's and Parkinson's diseases
Objective: This study tested the hypothesis that markers of functional cortical source connectivity of resting state eyes-closed electroencephalographic (rsEEG) rhythms may be abnormal in subjects with mild cognitive impairment due to Alzheimer's (ADMCI) and Parkinson's (PDMCI) diseases compared to healthy elderly subjects (Nold). Methods: rsEEG data had been collected in ADMCI, PDMCI, and Nold subjects (N = 75 for any group). eLORETA freeware estimated functional lagged linear connectivity (LLC) from rsEEG cortical sources. Area under receiver operating characteristic (AUROC) curve indexed the accuracy in the classification of Nold and MCI individuals. Results: Posterior interhemispheric and widespread intrahemispheric alpha LLC solutions were abnormally lower in both MCI groups compared to the Nold group. At the individual level, AUROC curves of LLC solutions in posterior alpha sources exhibited moderate accuracies (0.70-0.72) in the discrimination of Nold vs. ADMCI-PDMCI individuals. No differences in the LLC solutions were found between the two MCI groups. Conclusions: These findings unveil similar abnormalities in functional cortical connectivity estimated in widespread alpha sources in ADMCI and PDMCI. This was true at both group and individual levels. Significance: The similar abnormality of alpha source connectivity in ADMCI and PDMCI subjects might reflect common cholinergic impairment. (C) 2018 International Federation of Clinical Neurophysiology. Published by Elsevier B.V. All rights reserved
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