Para bem formar uma personalidade normal. Discursos sobre como educar as crianças em revistas de Educação Familiar (1945-1958)

Este artigo tem como objeto de análise os discursos acerca do desenvolvimento socioemocional, veiculados por meio das revistas de ‘educação familiar’ publicadas em Portugal, entre 1945 e 1958, período em que se assiste a uma maior preocupação, por parte de médicos e educadores, com a preservação da saúde mental da criança. A análise realizada permitiu compreender que o desenvolvimento socioemocional é construído, pelos colaboradores das revistas, como algo problemático, cuja normalidade é constantemente ameaçada pelas ações incorretas dos pais. Tal constitui um poderoso argumento para uma crescente intervenção dos peritos psi na vida familiar, que se vão constituir, durante a segunda metade do século XX, em elementos centrais do processo de governação da parentalidade e da infância

Implementation of a Practical Teaching Course on Protein Engineering

Simple Summary Proteins are the workhorses of the cell. With different combinations of the 20 common amino acids and some modifications of these amino acids, proteins have evolved with a staggering array of new functions and capabilities due to Protein Engineering techniques. The practical course presented was offered to undergraduate bioengineering and chemical students at the Faculty of Engineering of the University of Porto (Portugal) and consists of sequential laboratory sessions to learn the basic skills related to the expression and purification of recombinant proteins in bacterial hosts. These experiments were successfully applied by students as all working groups were able to isolate a model recombinant protein (the enhanced green fluorescent protein) from a cell lysate containing a mixture of proteins and other biomolecules produced by an Escherichia coli strain and evaluate the performance of the extraction and purification procedures they learned. Protein Engineering is a highly evolved field of engineering aimed at developing proteins for specific industrial, medical, and research applications. Here, we present a practical teaching course to demonstrate fundamental techniques used to express, purify and analyze a recombinant protein produced in Escherichia coli-the enhanced green fluorescent protein (eGFP). The methodologies used for eGFP production were introduced sequentially over six laboratory sessions and included (i) bacterial growth, (ii) sonication (for cell lysis), (iii) affinity chromatography and dialysis (for eGFP purification), (iv) bicinchoninic acid (BCA) and fluorometry assays for total protein and eGFP quantification, respectively, and (v) sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for qualitative analysis. All groups were able to isolate the eGFP from the cell lysate with purity levels up to 72%. Additionally, a mass balance analysis performed by the students showed that eGFP yields up to 46% were achieved at the end of the purification process following the adopted procedures. A sensitivity analysis was performed to pinpoint the most critical steps of the downstream processing

O conhecimento e o uso de suplementos alimentares em escolares

Orientadora : Profª Fernanda Cassanho TeodoroMonografia (especialização) - Universidade Federal do Paraná, Setor de Ciências da Saúde, Curso de Especialização em Saúde para professores do ensino Fundamental e Médio. Núcleo de Educação a Distância.Inclui referênciasResumo : A escolha da academia, a preocupação com a aparência, o exercício do corpo e o seguimento de dieta adequada são velhos hábitos herdados da Grécia antiga e almejados por muitas pessoas. Tem se difundido a utilização de suplementos, compostos à base de carboidratos, lipídios, vitaminas e minerais, e principalmente aminoácidos, comercializados e consumidos indiscriminadamente, sem orientação competente, sem que se conheçam seus efeitos, muitos dos quais ainda estão sob investigação. A justificativa por esse trabalho deu-se observando os dias atuais, que estão em "alta" as propagandas e a preocupação excessiva da mídia em relação ao padrão de beleza e ao corpo de jovens e adolescentes, surgindo a preocupação em relação ao uso indiscriminado de suplementos alimentares entre adolescentes e jovens do ensino médio do colégio em que trabalho. O presente trabalho foi desenvolvido através da metodologia roda de conversa e teve como objetivo implementar atividades que propiciassem a reflexão crítica dos alunos dos 2º e 3º anos do Ensino Médio do Colégio Estadual Jardim Universitário da cidade de Sarandi, PR, sobre o conhecimento e uso dos suplementos alimentares, através de questionário, manhãs de leitura, seminários e rodas de conversa. Através das atividades aplicadas concluiu-se que diante das considerações tratar das questões dos suplementos alimentares na escola foi fundamental para o conhecimento dos alunos, pois é algo que está presente no dia a dia, pois devido a maioria das respostas dos questionários, observou-se que os alunos citados na pesquisa se preocupam muito com a mídia e os padrões de beleza, onde exige do adolescente uma aparência bonita e um corpo "perfeito"

Transitional Care Management from Emergency Services to Communities: An Action Research Study

In recent years, nurses have developed projects in the area of hospital to community transition. The objective of the present study was to analyze the transitional care offered to elderly people after they used emergency services and were discharged to return to the community. The action research method was chosen. The participants were nurses, elderly people 70 years old or older, and their caregivers. The study was carried out from October 2018 to August 2019. The data were collected by means of semi-structured interviews with the nurses, analysis of medical records, participatory observation, phone calls to the elderly people and caregivers, and team meetings. The qualitative data were submitted to Bardin’s content analysis. Statistical treatment was carried out by applying SPSS version 23.0. The institution’s research ethics committee approved the research. Only 31.4% of the sample experienced care continuity after discharge, and the rate of readmission to emergency services during the first 30 days after discharge was 33.4%. The referral letters lacked data on information provided to patients or caregivers, and nurses mentioned difficulties in communication between care levels, as well as obstacles to teamwork; they also mentioned that the lack of health policies and clinical rules to formalize transitional care between the hospital and the community perpetuated non-coordination of care between the two contexts. The low level of literacy of patients and their relatives are mentioned as a cause for not understanding the information regarding seeking primary health care services and handing the discharge letter. It was concluded that there is an urgent need to mobilize health teams toward action in the patients’ process of returning home, and this factor must be taken into account in care planning.info:eu-repo/semantics/publishedVersio

Sexualidade na adolescência : diagnóstico de situação de Penalva do Castelo

A adolescência é marcada pela descoberta da sexualidade, podendo conduzir a problemas de saúde pública como a gravidez na adolescência e as infecções sexualmente transmissíveis. Assim, com o apoio do Centro de Saúde local desenvolveu-se um diagnóstico de situação dos adolescentes de Penalva do Castelo, com o intuito de identificar os principais factores de risco, conhecimentos e comportamentos associados à sexualidade na adolescência. Espera-se, no futuro, delinear um plano de intervenção sustentado que permita alcançar ganhos em saúde sensíveis aos cuidados de enfermagem

FERNANDO PESSOA E A “ALGIBEIRA VAZIA”. ESTUDO IMAGÉTICO DO POEMA ANIVERSÁRIO

É no tema da infância que Fernando Pessoa mais revela a gênese do seu processo heteronímico, identificador maior de sua obra poética. É em Álvaro de Campos, o heterônimo que mais explora esse tema, a sua manifestação do sentir mais intensamente, a saudade da sua infância. O poema “Aniversário”, escrito em 1929, será o nosso objeto de estudo, para ilustrar a fase do “engenheiro aposentado”, detectando e analisando a sua composição imagética ao fazer uma comparação entre o passado feliz e o presente melancólico

Gneuropathy: Validation process at clinical environment

Spinal cord injuries are one of the most traumatic situations with a major impact on a person's quality of life. This type of injury have a extremely impact in the performance of daily life activities not only due to motor alterations but also due to the appearance of neuropathic pain Throughout the rehabilitation process the evaluation and intervention methodologies are not very systematic and are not personalized. Thus, to bridge this gap, the VR4NeuroPain was developed a technology that associates virtual reality with a glove "GNeuroPathy". The glove "GNeuroPathy" allows the collection of physiological parameters, namely to identify the electrodermic activity (EDA) while the patient carries out activities in an immersive environment. The main objective of this article is to present the validation process of the "GNeuroPathy" in clinical context. "GNeuroPathy" was applied to a group of 17 individuals with incomplete spinal cord injury. The results showed that "GNeuroPathy" is easy to apply and is suitable for comfort and texture. Data were also collected from EDA and it was found that there is a significant difference in signal amplitude in patients with low and high functionality.preprintpublishe

The importance of phenotyping legumes at multiple levels in order to mitigate climate change scenarios and help address sustainable development goals

info:eu-repo/semantics/publishedVersio

“ESTRANGEIRO AQUI COMO EM TODA A PARTE” UMA ABORDAGEM DOS POEMAS “LISBON REVISITED”, DE FERNANDO PESSOA.

Nostalgia of childhood marks the poetry of Fernando Pessoa, as relating to mythical and idealization. The objective of this research is to analyze the poems Lisbon Revisited (1923) and Lisbon Revisited (1926) by Alvaro de Campos, in his imagistic components, his revealed confrontations of the emptiness of the self and the connection with the poetic process that links Modernism to memory. Examining the explicit confrontation with the city of Lisbon after generalizing the figure of the foreigner to all the needs of involvement and social commitment (poem, 1923). Interpreting the poems, since the strangeness of the title and the need to inquire about the process of heteronomy, nihilism and the passivity, despite the connection to memory, (poem, 1926). A “saudade da infância” marca a poesia de Fernando Pessoa, no que ela tem de mítica e de idealização. O objetivo dessa pesquisa é analisar os poemas “Lisbon Revisited” (1923) e “Lisbon Revisited” (1926) de Álvaro de Campos, em suas componentes imagéticas, seus confrontos reveladores do esvaziamento do eu, e da ligação com o processo poético que liga o Modernismo à memória. Examinar o confronto explícito com a cidade de Lisboa depois de generalizar a figura do estrangeiro a todas as necessidades de envolvimento e compromisso social, (poema de 1923). Interpretar os poemas, desde o estranhamento do título e a necessidade de inquirir sobre o processo da heteronímia, o niilismo e a passividade, apesar da ligação com a memória, (poema de 1926). &nbsp

Learning from Online Voices: A Mixed Methods Approach to Explore Patient Online Reviews of Hospital Care in Portugal

Online patient reviews can offer a rich information source to users of healthcare services, as well as for hospital management and quality monitoring. Whereas in recent years the volume of online patient reviews has been consistently growing, organizations still lack standardized approaches and tools to allow for the systematic monitoring of users’ online comments. Therefore, managers are lagging in the ability to make use of such data from patients’ voices for improving the quality of the services provided. If organizations fail to develop the right capabilities to consider users’ online reviews and feedback, they risk not only to miss important quality failure alerts, as wells as to frustrate their customers’ expectations for service and attention. In this chapter, we present a qualitative analysis of patients’ reviews for healthcare services in Portugal, building on a sample of data extracted from Google for the year of 2019. The chapter reports the major quality management themes addressed by hospital users in their online expressions and offers some guidelines to support a structured analysis and visualization of results from online users’ word of mouth data