Integrated luminometer for the determination of trace metals in seawater using fluorescence, phosphorescence and chemiluminescence detection

The paper describes an integrated luminometer able to perform fluorescence (FL), room temperature phosphorescence (RTP) and chemiluminescence (CL) measurements on seawater samples. The technical details of the instrumentation are presented together with flow injection (FI) manifolds for the determination of cadmium and zinc (by FL), lead (RTP) and cobalt (CL). The analytical figures of merit are given for each manifold and results are presented for the determination of the four trace metals in seawater reference materials (NASS-5, SLEW-2) and Scheldt estuarine water samples

Numerical Modelling of Masonry Arches Strengthened with SFRM

The adoption of effective strengthening techniques of historical constructions is one of the most widely debated aspects in structural engineering. Within this topic, the application of steel fiber reinforced mortar (SFRM) has been recently proposed as a low invasive and effective way to obtain a considerable structural benefit in the safety of existing masonry structure. To this purpose, in this paper the experimental results obtained on a circular masonry arches are presented. The considered specimens, subjected to a vertical increasing static load, is tested in the unstrengthened and strengthened configurations, and is part of a wider experimental campaign. After presenting and discussing the experimental results, they are compared with those relative to numerical simulations conducted by means of a discrete macro-element (DME) strategy, based on a simple mechanical scheme, able to model the nonlinear behavior of masonry structures with a limited computational effort. Such an approach is here extended to model the SFRM strengthening technique accounting for the main failure mechanisms associated to the combined presence existing masonry and the additional strengthening layer applied at the intrados of the arch. Numerical and experimental results demonstrate the efficacy of the proposed retrofitting strategy both in terms of bearing capacity and increase of ductility

An outflow powers the optical rise of the nearby, fast-evolving tidal disruption event AT2019qiz

At 66 Mpc, AT2019qiz is the closest optical tidal disruption event (TDE) to date, with a luminosity intermediate between the bulk of the population and iPTF16fnl. Its proximity allowed a very early detection and triggering of multiwavelength and spectroscopic follow-up well before maximum light. The velocity dispersion of the host galaxy and fits to the TDE light curve indicate a black hole mass $\approx 10^6$ M$_\odot$, disrupting a star of $\approx 1$ M$_\odot$. Comprehensive UV, optical and X-ray data shows that the early optical emission is dominated by an outflow, with a luminosity evolution $L \propto t^2$, consistent with a photosphere expanding at constant velocity ($\gtrsim 2000$ km s$^{-1}$), and a line-forming region producing initially blueshifted H and He II profiles with $v=3000-10000$ km s$^{-1}$. The fastest optical ejecta approach the velocity inferred from radio detections (modelled in a forthcoming companion paper from K.~D.~Alexander et al.), thus the same outflow may be responsible for both the fast optical rise and the radio emission -- the first time this connection has been observed in a TDE. The light curve rise begins $29 \pm 2$ days before maximum light, peaking when the photosphere reaches the radius where optical photons can escape. The photosphere then undergoes a sudden transition, first cooling at constant radius then contracting at constant temperature. At the same time, the blueshifts disappear from the spectrum and Bowen fluorescence lines (N III) become prominent, implying a source of far-UV photons, while the X-ray light curve peaks at $\approx 10^{41}$ erg s$^{-1}$. Assuming that these X-rays are from prompt accretion, the size and mass of the outflow are consistent with the reprocessing layer needed to explain the large optical to X-ray ratio in this and other optical TDEs, possibly favouring accretion-powered over collision-powered outflow models.Comment: Accepted for publication in MNRA

A 12-year-old girl with absent radial pulse: arterial thoracic outlet syndrome with subclavian artery aneurysm and thrombosis of the brachial artery

Brachial arterial occlusion is rare in children and adolescents. Once a traumatic cause is excluded, the differential diagnosis consists of a variety of rare conditions. We report the case of a 12-year-old girl whose presenting symptoms--an absent radial pulse and Raynaud's phenomenon of the right hand--could be easily mistaken for a vasculitis. She was found to have arterial thoracic outlet syndrome with right subclavian artery compression and aneurysm formation caused by an anomalous first rib and consecutive thromboembolic occlusion of the brachial artery. The diagnosis and differential diagnosis of this condition are reviewed

Mass spectrometric analysis of the active site tryptic peptide of recombinant O6-methylguanine-DNA methyltransferase following incubation with human colorectal DNA reveals the presence of an O6-alkylguanine adductome

Human exposure to DNA alkylating agents is poorly characterized, partly because only a limited range of specific alkyl DNA adducts have been quantified. The human DNA repair protein, O6-methylguanine O6-methyltransferase (MGMT), irreversibly transfers the alkyl group from DNA O6-alkylguanines (O6-alkGs) to an acceptor cysteine, allowing the simultaneous detection of multiple O6-alkG modifications in DNA by mass spectrometric analysis of the MGMT active site peptide (ASP). Recombinant MGMT was incubated with oligodeoxyribonucleotides (ODNs) containing different O6-alkGs, Temozolomide-methylated calf thymus DNA (Me-CT-DNA), or human colorectal DNA of known O6-MethylG (O6-MeG) levels. It was digested with trypsin, and ASPs were detected and quantified by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry. ASPs containing S-methyl, S-ethyl, S-propyl, S-hydroxyethyl, S-carboxymethyl, S-benzyl, and S-pyridyloxobutyl cysteine groups were detected by incubating MGMT with ODNs containing the corresponding O6-alkGs. The LOQ of ASPs containing S-methylcysteine detected after MGMT incubation with Me-CT-DNA was <0.05 pmol O6-MeG per mg CT-DNA. Incubation of MGMT with human colorectal DNA produced ASPs containing S-methylcysteine at levels that correlated with those of O6-MeG determined previously by HPLC-radioimmunoassay (r2 = 0.74; p = 0.014). O6-CMG, a putative O6-hydroxyethylG adduct, and other potential unidentified MGMT substrates were also detected in human DNA samples. This novel approach to the identification and quantitation of O6-alkGs in human DNA has revealed the existence of a human DNA alkyl adductome that remains to be fully characterized. The methodology establishes a platform for characterizing the human DNA O6-alkG adductome and, given the mutagenic potential of O6-alkGs, can provide mechanistic information about cancer pathogenesis

An outflow powers the optical rise of the nearby, fast-evolving tidal disruption event AT2019qiz

At 66 Mpc, AT2019qiz is the closest optical tidal disruption event (TDE) to date, with a luminosity intermediate between the bulk of the population and the faint-and-fast event iPTF16fnl. Its proximity allowed a very early detection and triggering of multiwavelength and spectroscopic follow-up well before maximum light. The velocity dispersion of the host galaxy and fits to the TDE light curve indicate a black hole mass ≈106 M, disrupting a star of ≈1 M. By analysing our comprehensive UV, optical, and X-ray data, we show that the early optical emission is dominated by an outflow, with a luminosity evolution L ∝ t 2, consistent with a photosphere expanding at constant velocity (2000 km s−1), and a line-forming region producing initially blueshifted H and He II profiles with v = 3000–10 000 km s−1. The fastest optical ejecta approach the velocity inferred from radio detections (modelled in a forthcoming companion paper from K. D. Alexander et al.), thus the same outflow may be responsible for both the fast optical rise and the radio emission – the first time this connection has been observed in a TDE. The light-curve rise begins 29 ± 2 d before maximum light, peaking when the photosphere reaches the radius where optical photons can escape. The photosphere then undergoes a sudden transition, first cooling at constant radius then contracting at constant temperature. At the same time, the blueshifts disappear from the spectrum and Bowen fluorescence lines (N III) become prominent, implying a source of far-UV photons, while the X-ray light curve peaks at ≈1041 erg s−1. Assuming that these X-rays are from prompt accretion, the size and mass of the outflow are consistent with the reprocessing layer needed to explain the large optical to X-ray ratio in this and other optical TDEs, possibly favouring accretion-powered over collision-powered outflow models

A detailed spectroscopic study of tidal disruption events

Spectroscopically, tidal disruption events (TDEs) are characterized by broad (∼104 km s−1) emission lines and show a large diversity as well as different line profiles. After carefully and consistently performing a series of data reduction tasks including host galaxy light subtraction, we present here the first detailed, spectroscopic population study of 16 optical and UV TDEs. We study a number of emission lines prominent among TDEs including Hydrogen, Helium, and Bowen lines and we quantify their evolution with time in terms of line luminosities, velocity widths, and velocity offsets. We report a time lag between the peaks of the optical light curves and the peak luminosity of Hα spanning between ∼7 and 45 days. If interpreted as light echoes, these lags correspond to distances of ∼2 − 12 × 1016 cm, which are one to two orders of magnitudes larger than the estimated blackbody radii (RBB) of the same TDEs and we discuss the possible origin of this surprisingly large discrepancy. We also report time lags for the peak luminosity of the He I 5876 Å line, which are smaller than the ones of Hα for H TDEs and similar or larger for N III Bowen TDEs. We report that N III Bowen TDEs have lower Hα velocity widths compared to the rest of the TDEs in our sample and we also find that a strong X-ray to optical ratio might imply weakening of the line widths. Furthermore, we study the evolution of line luminosities and ratios with respect to their radii (RBB) and temperatures (TBB). We find a linear relationship between Hα luminosity and the RBB (Lline ∝ RBB) and potentially an inverse power-law relation with TBB (Lline ∝ TBB−β), leading to weaker Hα emission for TBB ≥ 25 000 K. The He II/He I ratio becomes large at the same temperatures, possibly pointing to an ionization effect. The He II/Hα ratio becomes larger as the photospheric radius recedes, implying a stratified photosphere where Helium lies deeper than Hydrogen. We suggest that the large diversity of the spectroscopic features seen in TDEs along with their X-ray properties can potentially be attributed to viewing angle effects

A multi-centre polyp detection and segmentation dataset for generalisability assessment

Polyps in the colon are widely known cancer precursors identified by colonoscopy. Whilst most polyps are benign, the polyp’s number, size and surface structure are linked to the risk of colon cancer. Several methods have been developed to automate polyp detection and segmentation. However, the main issue is that they are not tested rigorously on a large multicentre purpose-built dataset, one reason being the lack of a comprehensive public dataset. As a result, the developed methods may not generalise to different population datasets. To this extent, we have curated a dataset from six unique centres incorporating more than 300 patients. The dataset includes both single frame and sequence data with 3762 annotated polyp labels with precise delineation of polyp boundaries verified by six senior gastroenterologists. To our knowledge, this is the most comprehensive detection and pixel-level segmentation dataset (referred to as PolypGen) curated by a team of computational scientists and expert gastroenterologists. The paper provides insight into data construction and annotation strategies, quality assurance, and technical validation

Human papilloma viruses and cervical tumours: mapping of integration sites and analysis of adjacent cellular sequences

BACKGROUND: In cervical tumours the integration of human papilloma viruses (HPV) transcripts often results in the generation of transcripts that consist of hybrids of viral and cellular sequences. Mapping data using a variety of techniques has demonstrated that HPV integration occurred without obvious specificity into human genome. However, these techniques could not demonstrate whether integration resulted in the generation of transcripts encoding viral or viral-cellular sequences. The aim of this work was to map the integration sites of HPV DNA and to analyse the adjacent cellular sequences. METHODS: Amplification of the INTs was done by the APOT technique. The APOT products were sequenced according to standard protocols. The analysis of the sequences was performed using BLASTN program and public databases. To localise the INTs PCR-based screening of GeneBridge4-RH-panel was used. RESULTS: Twelve cellular sequences adjacent to integrated HPV16 (INT markers) expressed in squamous cell cervical carcinomas were isolated. For 11 INT markers homologous human genomic sequences were readily identified and 9 of these showed significant homologies to known genes/ESTs. Using the known locations of homologous cDNAs and the RH-mapping techniques, mapping studies showed that the INTs are distributed among different human chromosomes for each tumour sample and are located in regions with the high levels of expression. CONCLUSIONS: Integration of HPV genomes occurs into the different human chromosomes but into regions that contain highly transcribed genes. One interpretation of these studies is that integration of HPV occurs into decondensed regions, which are more accessible for integration of foreign DNA