Heterotrophy as a tool to overcome the long and costly autotrophic scale-up process for large scale production of microalgae

Industrial scale-up of microalgal cultures is often a protracted step prone to culture collapse and the occurrence of unwanted contaminants. To solve this problem, a two-stage scale-up process was developed - heterotrophically Chlorella vulgaris cells grown in fermenters (1st stage) were used to directly inoculate an outdoor industrial autotrophic microalgal production unit (2nd stage). A preliminary pilot-scale trial revealed that C. vulgaris cells grown heterotrophically adapted readily to outdoor autotrophic growth conditions (1-m3 photobioreactors) without any measurable difference as compared to conventional autotrophic inocula. Biomass concentration of 174.5 g L-1, the highest value ever reported for this microalga, was achieved in a 5-L fermenter during scale-up using the heterotrophic route. Inocula grown in 0.2- and 5-m3 industrial fermenters with mean productivity of 27.54 ± 5.07 and 31.86 ± 2.87 g L-1 d-1, respectively, were later used to seed several outdoor 100-m3 tubular photobioreactors. Overall, all photobioreactor cultures seeded from the heterotrophic route reached standard protein and chlorophyll contents of 52.18 ± 1.30% of DW and 23.98 ± 1.57 mg g-1 DW, respectively. In addition to providing reproducible, high-quality inocula, this two-stage approach led to a 5-fold and 12-fold decrease in scale-up time and occupancy area used for industrial scale-up, respectively.Agência financiadora project FERMALG 017608 Fundacao para a Ciencia e a Tecnologia (FCT) UID/Multi/04326/2019 project FERMALG (AVISO) 32/SI/2015info:eu-repo/semantics/publishedVersio

Combining static and dynamic analysis for the reverse engineering of web applications

Software has become so complex that it is increasingly hard to have a complete understanding of how a particular system will behave. Web applications, their user interfaces in particular, are built with a wide variety of technologies making them particularly hard to debug and maintain. Reverse engineering techniques, either through static analysis of the code or dynamic analysis of the running application, can be used to help gain this understanding. Each type of technique has its limitations. With static analysis it is difficult to have good coverage of highly dynamic applications, while dynamic analysis faces problems with guaranteeing that generated models fully capture the behavior of the system. This paper proposes a new hybrid approach for the reverse engineering of web applications' user interfaces. The approach combines dynamic analyzes of the application at runtime, with static analyzes of the source code of the event handlers found during interaction. Information derived from the source code is both directly added to the generated models, and used to guide the dynamic analysis.This work is funded by ERDF - European Regional Development Fund through the COMPETE Programme (operational programme for competitiveness) and by National Funds through the FCT - Fundação para a Ciência e a Tecnologia (Portuguese Foundation for Science and Technology) within project FCOMP-01-0124-FEDER-015095. Carlos Eduardo Silva is further funded by the Portuguese Government through FCT, grant SFRH/BD/71136/2010

Can GUI implementation markup languages be used for modelling?

The current diversity of available devices and form factors increases the need for model-based techniques to support adapting applications from one device to another.Most work on user interface modelling is built around declarative markup languages. Markup languages play a relevant role, not only in the modelling of user interfaces, but also in their implementation. However, the languages used by each community (modellers/ developers) have, to a great extent evolved separately. This means that the step from concrete model to final interface becomes needlessly complicated, requiring either compilers or interpreters to bridge this gap. In this paper we compare a modelling language (UsiXML) with several markup implementation languages. We analyse if it is feasible to use the implementation languages as modelling languages.(undefined

Characterizing the control logic of web applications’ user interfaces

In order to develop an hybrid approach to the Reverse Engineer of Web applications, we need first to understand how much of the control logic of the user interface can be obtained from the analysis of event listeners. To that end, we have developed a tool that enables us to perform such analysis, and applied it to the implementation of the one thousand most widely used Websites (according to Alexa Top Sites). This paper describes our approach for analyzing the user interface layer of those Websites, and the results we got from the analysis. The conclusions drawn from the exercise will be used to guide the development of the proposed hybrid reverse engineering tool.This work was partly funded by project LATiCES (Ref. NORTE-07-0124-FEDER-000062) financed by the North Portugal Regional Operational Programme (ON.2 – O Novo Norte), under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF), and by national funds, through the Portuguese funding agency, Fundação para a Ciência e a Tecnologia (FCT). Carlos Eduardo Silva is further funded by the Portuguese Government through FCT, grant SFRH/BD/71136/2010

Constraining Elko Dark Matter at the LHC with Monophoton Events

A mass dimension one fermion, also known as Elko, constitutes a dark matter candidate which might interact with photons at the tree level in a specific fashion. In this work, we investigate the constraints imposed by unitarity and LHC data on this type of interactions using the search for new physics in monophoton events. We found that Elkos which can explain the dark matter relic abundance mainly through electromagnetic interactions are excluded at the 95\%CL by the 8 TeV LHC data for masses up to 1 TeV.Comment: 6 pages, 4 figure

Choroidal and retinal structural, cellular and vascular changes in a rat model of Type 2 diabetes

Increasing evidence points to inflammation as a key factor in the pathogenesis of diabetic retinopathy (DR). Choroidal changes in diabetes have been reported and several attempts were made to validate in vivo choroidal thickness (CT) as a marker of retinopathy. We aimed to study choroidal and retinal changes associated with retinopathy in an animal model of spontaneous Type 2 diabetes, Goto-Kakizaki (GK) rats. Sclerochoroidal whole mounts and cryosections were prepared from 52-week-old GK and age-matched control Wistar Han rats. CT was measured by optical coherence tomography. Microglia reactivity, pericyte and endothelial cells distribution, and immunoreactivity of vascular endothelial growth factor (VEGF) and VEGF receptor 2 (VEGFR2) were evaluated by immunofluorescence. Choroidal vessels were visualized by direct perfusion with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (Dil). Choroidal vascular density was evaluated by fluorescence microscopy. GK rats had increased CT (58.40 ± 1.15 μm versus 50.90 ± 1.58 μm, p < 0.001), reduced vascular density of the choriocapillaris (CC) (p = 0.045), increased Iba1+ cells density in the outer retina (p = 0.003) and increased VEGFR2 immunoreactivity in most retinal layers (p = 0.021 to 0.037). Choroidal microglial cells and pericytes showed polarity in their distribution, sparing the innermost choroid. This cell-free gap in the inner choroid was more pronounced in GK rats. In summary, GK rats have increased CT with decreased vascular density in the innermost choroid, increased VEGFR2 immunoreactivity in the retina and increased Iba1+ cells density in the outer retina.info:eu-repo/semantics/publishedVersio

Tuned liquid dampers simulation for earthquake response control of buildings

This paper is focused on the study of an earthquake protection system, the Tuned Liquid Damper (TLD), which can, if adequately designed, reduce earthquake demands on buildings. This positive effect is accomplished taking into account the oscillation of the free surface of a fluid inside a tank (sloshing). The behaviour of an isolated Tuned Liquid Damper, subjected to a sinusoidal excitation at its base, with different displacement amplitudes, was studied by finite element analysis. The efficiency of the TLD in improving the seismic response of an existing building, representative of modern architecture buildings in southern European countries was also evaluated based on linear dynamic analyses

GUIsurfer : a reverse engineering framework for user interface software

GUIsurfer: A Reverse Engineering Framework for User Interface Softwar

Combining formal methods and functional strategies regarding the reverse engineering of interactive applications

Graphical user interfaces (GUIs) make software easy to use by providing the user with visual controls. Therefore, correctness of GUI’s code is essential to the correct execution of the overall software. Models can help in the evaluation of interactive applications by allowing designers to concentrate on its more important aspects. This paper describes our approach to reverse engineer an abstract model of a user interface directly from the GUI’s legacy code. We also present results from a case study. These results are encouraging and give evidence that the goal of reverse engineering user interfaces can be met with more work on this technique.Fundação para a Ciência e a Tecnologia (FCT) Fundo Europeu de Desenvolvimento Regional (FEDER