Urbanismo aruccitano: el trazado regulador de la domus norte

En el siguiente trabajo se presenta una propuesta del trazado regulador de la denominada Casa Norte de la ciudad de Arucci, uno de los elementos urbanos mejor conocidos en su planta arquitectónica. El estudio de su modulación y el trazado regulador que rigió su diseño definen diversas particularidades de este conjunto estructural como son la ruptura de su eje mayor, el acceso en uno de los lados mayores, así como otros elementos que la caracterizan. Con todo, se muestran diversos resultados que definen de una manera cada vez más nítida el trazado viario de la ciudad de un modo general y la arquitectura doméstica de la ciudad en particular

Comparison of surface integral equations for left-handed materials

A wide analysis of left-handed material (LHM) spheres with di®erent constitutive parameters has been carried out employ- ing di®erent integral-equation formulations based on the Method of Moments. The study is focused on the accuracy assessment of for- mulations combining normal equations (combined normal formula- tion, CNF), tangential equations (combined tangential formulation, CTF, and Poggio-Miller-Chang-Harrington-Wu-Tsai formulation, PM- CHWT) and both of them (electric and magnetic current combined ¯eld integral equation, JMCFIE) when dealing with LHM's. Relevant and informative features as the condition number, the eigenvalues dis- tribution and the iterative response are analyzed. The obtained results show up the suitability of the JMCFIE for this kind of analysis in con- trast with the unreliable behavior of the other approaches.Ministerio de Ciencia e Innovación | Ref. TEC2008-06714-C02-01Ministerio de Ciencia e Innovación | Ref. TEC2008-06714-C02-02Ministerio de Ciencia e Innovación | Ref. CSD2008-00068Xunta de Galicia | Ref. INCITE08PXIB322250P

Relación entre las alteraciones estructurales y el deterioro cognitivo en pacientes con Esclerosis Múltiple

Setzenes Jornades de Foment de la Investigació (Any 2011)Las causas del deterioro cognitivo en los pacientes de Esclerosis Múltiple (EM) son todavía desconocidas, así como la relación entre dicho deterioro y el daño cerebral observado con distintas técnicas de adquisición y análisis de neuroimagen. Objetivos Observar la distribución de la atrofia en sustancia gris y blanca en pacientes de EM con Deterioro Cognitivo (DC) y Sin Deterioro Cognitivo (SDC), así como su relación con variables clínicas y cognitivas Pacientes y métodos Se reclutaron para el estudio a 22 participantes control (edad= 32,22 +6,24) y a 75 pacientes diagnosticados de EM (edad= 40,06 +10,01). Todos los participantes fueron valorados con la Batería Neuropsicológica Breve (BNB) especifica para valorar deterioro cognitivo en EM. Los pacientes fueron seleccionados en dos grupos: 1) DC: los que presentaban al menos 1 test de la batería por debajo de 2 DT (n=41); 2) SDC: rendimiento normal en pruebas neuropsicológicas (n=34). Posteriormente se adquirieron los datos morfométricos de todos los participantes en una Resonancia Magnética Siemens Avanto 1.5 T. Se obtuvieron parámetros de volumen en sustancia blanca y gris utilizando el programa de análisis Diffeomorphic Anatomical Registrations Through Exponentiated Lie Algebra (DARTEL). Resultados Los pacientes con DC muestran un menor rendimiento cognitivo en todas las pruebas neuropsicológicas. Estos pacientes también muestran más atrofia en distintas zonas corticales y subcorticales tanto en sustancia blanca como en sustancia gris respecto al grupo control y respecto al grupo de pacientes SDC. Finalmente, se observa una relación entre el rendimiento neuropsicológico en distintas pruebas cognitivas y la atrofia en sustancia gris en el grupo de pacientes con DC. Conclusiones: Se observa en pacientes de EM con DC un mayor grado de atrofia así como una consistente relación entre la atrofia en sustancia gris y rendimiento en pruebas neuropsicológicas

Solution of large-scale plasmonic problems with the multilevel fast multipole algorithm

A surface integral equation together with the multilevel fast multipole algorithm is successfully applied to fast and accurate resolution of plasmonic problems involving a large number of unknowns. The absorption, scattering, and extinction efficiencies of several plasmonic gold spheres of increasing size are efficiently obtained solving the elec- tric andmagnetic current combined-field integral equation. The numerical predictions are compared with reference analytic results to demonstrate the accuracy, suitability, and capabilities of this approach when dealing with large-scale plasmonic problems

Antioxidant responses related to temozolomide resistance in glioblastoma

Ministerio de Ciencia, Innovación y Universidades; Universidad de Málaga CBUA.Glioblastoma remains one of the most challenging and devastating cancers, with only a very small proportion of patients achieving 5-year survival. The current standard of care consists of surgery, followed by radiation therapy with concurrent and maintenance chemotherapy with the alkylating agent temozolomide. To date, this drug is the only one that provides a significant survival benefit, albeit modest, as patients end up acquiring resistance to this drug. As a result, tumor progression and recurrence inevitably occur, leading to death. Several factors have been proposed to explain this resistance, including an upregulated antioxidant system to keep the elevated intracellular ROS levels, a hallmark of cancer cells, under control. In this review, we discuss the mechanisms of chemoresistance -including the important role of glioblastoma stem cells-with emphasis on antioxidant defenses and how agents that impair redox balance (i.e.: sulfasalazine, erastin, CB-839, withaferin, resveratrol, curcumin, chloroquine, and hydroxychloroquine) might be advantageous in combined therapies against this type of cancer.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Changes in the nuclear proteomic profiling of human glioblastoma cells after glutaminase overexpression

A proteomics study of the nuclear protein profiling of human glioblastoma cells with targeted glutaminase expression. The study was performed with protein microarrays and SELDI-TOF technology.Human glutaminase (GA) enzymes are the products of two genes, GLS and GLS2. GA isozymes play markedly different roles in tumour biology: GLS isoforms (KGA and GAC) are related to cell growth and proliferation, whereas GLS2 isoforms (GAB and LGA) are associated with low proliferation rates and are characteristics for resting or quiescent cells. Furthermore, the GAB isoform has been recently involved in transcriptional regulation. Due to the proposed roles of GAB isoform in cellular differentiation and transcriptional modulation, the aim of the present study is to profile differentially expressed nuclear proteins in order to discover putative biomarkers associated with GAB overexpression in glioma cells. Nuclear cell proteins were incubated with strong anion exchange (Q10) and weak cation exchange (CM10) ProteinChip arrays and analyzed using surface-enhanced laser desorption/ionization time-of- flight mass spectrometry (SELDI-TOF/MS) proteomics technology. T98G-GAB nuclear protein expression profiles were compared with those of T98G-WT and empty vector transfected T98G-pcDNA3 with the ProteinChip Data Manager Client 4.0 software. Eighteen proteins were found to be differentially expressed between control cells and GAB-transfected T98G cells. Nine proteins with m/z between 5.5-29.7 were identified to be highly expressed in T98G-GAB (P≤ 0.01), while the expression of two proteins with m/z values of 7.8 and 8.5 were higher in control cells (P≤ 0.01). Our study shows the potential of proteomics profiling to get a deep insight into the role of nuclear GAB in brain tumors in order to assess its suitability as a novel anti-cancer therapeutic target. References Pérez-Gómez C, et al. Co-expression of glutaminase K and L isoenzymes in human tumour cells. Biochem. J. 2005; 386, 535-42. Szeliga M, et al. Transfection with liver-type glutaminase (LGA) cDNA alteres gene expression and reduces viability, migration and proliferation of T98G glioma cells. Glia 2009; 57, 1014-23.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.This work was supported by Grant SAF2010-17573 from the Ministry of Science and Innovation of Spain, Excellence Grant CVI-6656 from the regional Andalusian government (Junta de Andalucia), and Grant RD06/1012 of the RTA RETICS network from the Spanish Health Institute Carlos III

Comparison of surface integral equation formulations for electromagnetic analysis of plasmonic nanoscatterers

The performance of most widespread surface integral equation (SIE) formulations with the method of moments (MoM) are studied in the context of plasmonic materials. Although not yet widespread in optics, SIE-MoM approaches bring important advantages for the rigorous analysis of penetrable plasmonic bodies. Criteria such as accuracy in near and far field calculations, iterative convergence and reliability are addressed to assess the suitability of these formulations in the field of plasmonics.Ministerio de Ciencia e Innovación | Ref. TEC2011-28784-C02-01Ministerio de Ciencia e Innovación | Ref. TEC2011-28784-C02-0

Tanzawaic acids, a chemically novel set of bacterial conjugation inhibitors

Bacterial conjugation is the main mechanism for the dissemination of multiple antibiotic resistance in human pathogens. This dissemination could be controlled by molecules that interfere with the conjugation process. A search for conjugation inhibitors among a collection of 1,632 natural compounds, identified tanzawaic acids A and B as best hits. They specially inhibited IncW and IncFII conjugative systems, including plasmids mobilized by them. Plasmids belonging to IncFI, IncI, IncL/M, IncX and IncH incompatibility groups were targeted to a lesser extent, whereas IncN and IncP plasmids were unaffected. Tanzawaic acids showed reduced toxicity in bacterial, fungal or human cells, when compared to synthetic conjugation inhibitors, opening the possibility of their deployment in complex environments, including natural settings relevant for antibiotic resistance dissemination.Work in the FDLC group was supported by grants BFU2014-55534-C2-1-P from the Spanish Ministry of Economy and Competitiveness (http://www.mineco.gob.es) and 612146/FP7-ICT-2013-10 and 282004/FP7-HEALTH-2011-2.3.1-2 from the European Seventh Framework Programme (https://ec.europa.eu/research/fp7). MG was supported by a PhD fellowship from the University of Cantabria (http://www.unican.es). JCG was supported by a postdoctoral fellowship ASTF 402-2010 from the European Molecular Biology Organization (http://www.embo.org). Biomar Microbial Technologies was supported by grant 282004/FP7-HEALTH-2011-2.3.1-2 from the European Seventh Framework Programme (https://ec.europa.eu/research/fp7).USD 1,495 APC fee funded by the EC FP7 Post-Grant Open Access PilotPeer reviewe

Synthetic Fatty Acids Prevent Plasmid-Mediated Horizontal Gene Transfer

Bacterial conjugation constitutes a major horizontal gene transfer mechanism for the dissemination of antibiotic resistance genes among human pathogens. Antibiotic resistance spread could be halted or diminished by molecules that interfere with the conjugation process. In this work, synthetic 2-alkynoic fatty acids were identified as a novel class of conjugation inhibitors. Their chemical properties were investigated by using the prototype 2-hexadecynoic acid and its derivatives. Essential features of effective inhibitors were the carboxylic group, an optimal long aliphatic chain of 16 carbon atoms, and one unsaturation. Chemical modification of these groups led to inactive or less-active derivatives. Conjugation inhibitors were found to act on the donor cell, affecting a wide number of pathogenic bacterial hosts, including Escherichia, Salmonella, Pseudomonas, and Acinetobacter spp. Conjugation inhibitors were active in inhibiting transfer of IncF, IncW, and IncH plasmids, moderately active against IncI, IncL/M, and IncX plasmids, and inactive against IncP and IncN plasmids. Importantly, the use of 2-hexadecynoic acid avoided the spread of a derepressed IncF plasmid into a recipient population, demonstrating the feasibility of abolishing the dissemination of antimicrobial resistances by blocking bacterial conjugation.The work performed by the F.D.L.C. group was supported by grants BFU2011-26608 from the Spanish Ministry of Economy and Competitiveness and 612146/FP7-ICT-2013-10 and 282004/FP7-HEALTH-2011-2.3.1-2 from the European Seventh Framework Programme. The work performed by M.G. was supported by a Ph.D. fellowship from the University of Cantabria. The work performed by D.J.S.-R. was supported by the National Center for Research Resources and the National Institute of General Medical Sciences of the National Institutes of Health through grant no. 5P20GM103475-13 and the Interamerican University of Puerto Rico. The work performed by J.C.-G. was supported by an EMBO postdoctoral fellowship, ASTF 402-2010. The work performed by Biomar Microbial Technologies was supported by grant 282004/FP7-HEALTH-2011-2.3.1-2 from the European Seventh Framework Programme.USD 2,190 APC fee funded by the EC FP7 Post-Grant Open Access PilotPeer reviewe

Lysophosphatidic Acid and Glutamatergic Transmission

Signaling through bioactive lipids regulates nervous system development and functions. Lysophosphatidic acid (LPA), a membrane-derived lipid mediator particularly enriched in brain, is able to induce many responses in neurons and glial cells by affecting key processes like synaptic plasticity, neurogenesis, differentiation and proliferation. Early studies noted sustained elevations of neuronal intracellular calcium, a primary response to LPA exposure, suggesting functional modifications of NMDA and AMPA glutamate receptors. However, the crosstalk between LPA signaling and glutamatergic transmission has only recently been shown. For example, stimulation of presynaptic LPA receptors in hippocampal neurons regulates glutamate release from the presynaptic terminal, and excess of LPA induce seizures. Further evidence indicating a role of LPA in the modulation of neuronal transmission has been inferred from animal models with deficits on LPA receptors, mainly LPA1 which is the most prevalent receptor in human and mouse brain tissue. LPA1 null-mice exhibit cognitive and attention deficits characteristic of schizophrenia which are related with altered glutamatergic transmission and reduced neuropathic pain. Furthermore, silencing of LPA1 receptor in mice induced a severe down-regulation of the main glutaminase isoform (GLS) in cerebral cortex and hippocampus, along with a parallel sharp decrease on active matrix-metalloproteinase 9. The downregulation of both enzymes correlated with an altered morphology of glutamatergic pyramidal cells dendritic spines towards a less mature phenotype, indicating important implications of LPA in synaptic excitatory plasticity which may contribute to the cognitive and memory deficits shown by LPA1-deficient mice. In this review, we present an updated account of current evidence pointing to important implications of LPA in the modulation of synaptic excitatory transmission