484 research outputs found
sFlt-1/PlGF for prediction of early-onset pre-eclampsia: STEPS (Study of early pre-eclampsia in Spain)
Objective: A high ratio of soluble fmsâlike tyrosine kinaseâ1 (sFltâ1) to placental growth factor (PlGF) has been linked to preâeclampsia (PE). We evaluated the sFltâ1/PlGF ratio as a predictive marker for earlyâonset PE in women at risk of PE. Methods: This prospective, Spanish, multicenter study included pregnant women with a risk factor for PE, including intrauterine growth restriction, PE, eclampsia or hemolysis, elevated liver enzymes and low platelet count syndrome in previous pregnancy, pregestational diabetes or abnormal uterine artery Doppler. The primary objective was to show that the sFltâ1/PlGF ratio at 20, 24 and 28 weeks' gestation was predictive of earlyâonset PE (< 34 + 0 weeks). Serum sFltâ1 and PlGF were measured at 20, 24 and 28 weeks. Multivariate logistic regression was used to develop a predictive model. Results: A total of 819 women were enrolled, of which 729 were suitable for analysis. Of these, 78 (10.7%) women developed PE (24 early onset and 54 late onset). Median sFltâ1/PlGF ratio at 20, 24 and 28 weeks was 6.3 (interquartile range (IQR), 4.1â9.3), 4.0 (IQR, 2.6â6.3) and 3.3 (IQR, 2.0â5.9), respectively, for women who did not develop PE (controls); 14.5 (IQR, 5.5â43.7), 18.4 (IQR, 8.2â57.9) and 51.9 (IQR, 11.5â145.6) for women with earlyâonset PE; and 6.7 (IQR, 4.6â9.9), 4.7 (IQR, 2.8â7.2) and 6.0 (IQR, 3.8â10.5) for women with lateâonset PE. Compared with earlyâonset PE, the sFltâ1/PlGF ratio was significantly lower in controls (P < 0.001 at each timepoint) and in women with chronic hypertension (P < 0.001 at each timepoint), gestational hypertension (P < 0.001 at each timepoint) and lateâonset PE (P < 0.001 at each timepoint). A prediction model for earlyâonset PE was developed, which included the sFltâ1/PlGF ratio plus mean arterial pressure, being parous and previous PE, with areas under the receiverâoperating characteristics curves of 0.86 (95% CI, 0.77â0.95), 0.91 (95% CI, 0.85â0.97) and 0.93 (95% CI, 0.86â0.99) at 20, 24 and 28 weeks, respectively, and was superior to models using the sFltâ1/PlGF ratio alone or uterine artery mean pulsatility index. Conclusions: The sFltâ1/PlGF ratio can improve prediction of earlyâonset PE for women at risk of this condition
A Search for Dark Matter Annihilation with the Whipple 10m Telescope
We present observations of the dwarf galaxies Draco and Ursa Minor, the local
group galaxies M32 and M33, and the globular cluster M15 conducted with the
Whipple 10m gamma-ray telescope to search for the gamma-ray signature of
self-annihilating weakly interacting massive particles (WIMPs) which may
constitute astrophysical dark matter (DM). We review the motivations for
selecting these sources based on their unique astrophysical environments and
report the results of the data analysis which produced upper limits on excess
rate of gamma rays for each source. We consider models for the DM distribution
in each source based on the available observational constraints and discuss
possible scenarios for the enhancement of the gamma-ray luminosity. Limits on
the thermally averaged product of the total self-annihilation cross section and
velocity of the WIMP, , are derived using conservative estimates for
the magnitude of the astrophysical contribution to the gamma-ray flux. Although
these limits do not constrain predictions from the currently favored
theoretical models of supersymmetry (SUSY), future observations with VERITAS
will probe a larger region of the WIMP parameter phase space, and
WIMP particle mass (m_\chi).Comment: 33 pages, 12 figures, accepted for publication in the Astrophysical
Journa
Multiwavelength Observations of the Gamma-Ray Blazar PKS 0528+134 in Quiescence
We present multiwavelength observations of the ultraluminous blazar-type
radio loud quasar PKS 0528+134 in quiescence during the period July to December
2009. Significant flux variability on a time scale of several hours was found
in the optical regime, accompanied by a weak trend of spectral softening with
increasing flux. We suggest that this might be the signature of a contribution
from the accretion disk at the blue end of the optical spectrum. The optical
flux is weakly polarized with rapid variations of the degree and direction of
polarization, while the polarization of the 43 GHz radio core remains steady.
Optical spectropolarimetry suggests a trend of increasing degree of
polarization with increasing wavelength, providing additional evidence for an
accretion disc contribution towards the blue end of the optical spectrum. We
constructed four SEDs indicating that even in the quiescent state, the
bolometric luminosity of PKS 0528+134 is dominated by its gamma-ray emission. A
leptonic single-zone jet model produced acceptable fits to the SEDs with
contributions to the high-energy emission from synchrotron self-Compton
radiation and Comptonization of direct accretion disk emission. Fit parameters
close to equipartition were obtained. The moderate variability on long time
scales implies the existence of on-going particle acceleration, while the
observed optical polarization variability seems to point towards a turbulent
acceleration process. Turbulent particle acceleration at stationary features
along the jet therefore appears to be a viable possibility for the quiescent
state of PKS 0528+134.Comment: Accepted for Publication in The Astrophysical Journal. -
Acknowledgement adde
Accuracy of a method based on atomic absorption spectrometry to determine inorganic arsenic in food: outcome of the collaborative trial IMEP-41
A collaborative trial was conducted to determine the performance characteristics of an analytical method for the quantification of inorganic arsenic (iAs) in food. The method is based on (i) solubilisation of the protein matrix with concentrated hydrochloric acid to denature proteins and allow the release of all arsenic species into solution, and (ii) subsequent extraction of the inorganic arsenic present in the acid medium using chloroform followed by back-extraction to acidic medium. The final detection and quantification is done by flow injection hydride generation atomic absorption spectrometry (FI-HG-AAS). The seven test items used in this exercise were reference materials covering a broad range of matrices: mussels, cabbage, seaweed (hijiki), fish protein, rice, wheat, mushrooms, with concentrations ranging from 0.074 to 7.55 mg kg(-1). The relative standard deviation for repeatability (RSDr) ranged from 4.1 to 10.3%, while the relative standard deviation for reproducibility (RSDR) ranged from 6.1 to 22.8%. (C) 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Nanoinformatics: developing new computing applications for nanomedicine
Nanoinformatics has recently emerged to address the need of computing applications at the nano level. In this regard, the authors have participated in various initiatives to identify its concepts, foundations and challenges. While nanomaterials open up the possibility for developing new devices in many industrial and scientific areas, they also offer breakthrough perspectives for the prevention, diagnosis and treatment of diseases. In this paper, we analyze the different aspects of nanoinformatics and suggest five research topics to help catalyze new research and development in the area, particularly focused on nanomedicine. We also encompass the use of informatics to further the biological and clinical applications of basic research in nanoscience and nanotechnology, and the related concept of an extended ?nanotype? to coalesce information related to nanoparticles. We suggest how nanoinformatics could accelerate developments in nanomedicine, similarly to what happened with the Human Genome and other -omics projects, on issues like exchanging modeling and simulation methods and tools, linking toxicity information to clinical and personal databases or developing new approaches for scientific ontologies, among many others
The TeV spectrum of H1426+428
The BL Lac object H1426+428 was recently detected as a high energy gamma-ray
source by the VERITAS collaboration (Horan et al. 2002). We have reanalyzed the
2001 portion of the data used in the detection in order to examine the spectrum
of H1426+428 above 250 GeV. We find that the time-averaged spectrum agrees with
a power law of the shape dF/dE = 10^(-7.31 +- 0.15(stat) +- 0.16(syst)) x
E^(-3.50 +- 0.35(stat) +- 0.05(syst)) m^(-2)s^(-1)TeV^(-1) The statistical
evidence from our data for emission above 2.5 TeV is 2.6 sigma. With 95% c.l.,
the integral flux of H1426+428 above 2.5 TeV is larger than 3% of the
corresponding flux from the Crab Nebula. The spectrum is consistent with the
(non-contemporaneous) measurement by Aharonian et al. (2002) both in shape and
in normalization. Below 800 GeV, the data clearly favours a spectrum steeper
than that of any other TeV Blazar observed so far indicating a difference in
the processes involved either at the source or in the intervening space.Comment: LaTeX, 8 pages, 4 figures, accepted for publication in Ap
Detection of the BL Lac Object H1426+428 at TeV Gamma Ray Energies
A very high energy (VHE) gamma-ray signal has been detected at the 5.4 sigma
level from H1426+428, an x-ray selected BL Lacertae object at a redshift of
0.129. The object was monitored from 1995 - 1998 with the Whipple 10m imaging
atmospheric Cherenkov telescope as part of a general blazar survey; the results
of these observations, although not statistically significant, were
consistently positive. X-ray observations of H1426+428 during 1999 with the
BeppoSAX instrument revealed that the peak of its synchrotron spectrum occurs
at > 100 keV, leading to the prediction of observable TeV emission from this
object. H1426+428 was monitored extensively at the Whipple Observatory during
the 1999, 2000, and 2001 observing seasons. The strongest TeV signals were
detected in 2000 and 2001. During 2001, an integral flux of 2.04 +/- 0.35
x10e-11 cm-2 s-1 above 280 GeV was recorded from H1426+428. The detection of
H1426+428 supports the idea that, as also seen in Markarian 501 and
1ES2344+514, BL Lacertae objects with extremely high synchrotron peak
frequencies produce gamma rays in the TeV range.Comment: 35 pages, 7 figures, accepted by ApJ Two upper limits in Table 3
(upper limits for 1995 and 1997) are different from the ApJ versio
IT-supported integrated care pathways for diabetes: A compilation and review of good practices
Introduction: Integrated Care Pathways (ICPs) are a method for the mutual decision-making and organization of care for a well-defined group of patients during a well-defined period. The aim of a care pathway is to enhance the quality of care by improving patient outcomes, promoting patient safety, increasing patient satisfaction, and optimizing the use of resources. To describe this concept, different names are used, e.g. care pathways and integrated care pathways. Modern information technologies (IT) can support ICPs by enabling patient empowerment, better management, and the monitoring of care provided by multidisciplinary teams. This study analyses ICPs across Europe, identifying commonalities and success factors to establish good practices for IT-supported ICPs in diabetes care. Methods: A mixed-method approach was applied, combining desk research on 24 projects from the European Innovation Partnership on Active and Healthy Ageing (EIP on AHA) with follow-up interviews of project participants, and a non-systematic literature review. We applied a Delphi technique to select process and outcome indicators, derived from different literature sources which were compiled and applied for the identification of successful good practices. Results: Desk research identified sixteen projects featuring IT-supported ICPs, mostly derived from the EIP on AHA, as good practices based on our criteria. Follow-up interviews were then conducted with representatives from 9 of the 16 projects to gather information not publicly available and understand how these projects were meeting the identified criteria. In parallel, the non-systematic literature review of 434 PubMed search results revealed a total of eight relevant projects. On the basis of the selected EIP on AHA project data and non-systematic literature review, no commonalities with regard to defined process or outcome indicators could be identified through our approach. Conversely, the research produced a heterogeneous picture in all aspects of the projectsâ indicators. Data from desk research and follow-up interviews partly lacked information on outcome and performance, which limited the comparison between practices. Conclusion: Applying a comprehensive set of indicators in a multi-method approach to assess the projects included in this research study did not reveal any obvious commonalities which might serve as a blueprint for future IT-supported ICP projects. Instead, an unexpected high degree of heterogeneity was observed, that may reflect diverse local implementation requirements e.g. specificities of the local healthcare system, local regulations, or preexisting structures used for the project setup. Improving the definition of and reporting on project outcomes could help advance research on and implementation of effective integrated care solutions for chronic disease management across Europe
Search for High Energy Gamma Rays from an X-ray Selected Blazar Sample
Our understanding of blazars has been greatly increased in recent years by
extensive multi-wavelength observations, particularly in the radio, X-ray and
gamma-ray regions. Over the past decade the Whipple 10m telescope has
contributed to this with the detection of 5 BL Lacertae objects at very high
gamma-ray energies. The combination of multi-wavelength data has shown that
blazars follow a well-defined sequence in terms of their broadband spectral
properties. Together with providing constraints on emission models, this
information has yielded a means by which potential sources of TeV emission may
be identified and predictions made as to their possible gamma-ray flux. We have
used the Whipple telescope to search for TeV gamma-ray emission from eight
objects selected from a list of such candidates. No evidence has been found for
VHE emission from the objects in our sample, and upper limits have been derived
for the mean gamma-ray flux above 390GeV. These flux upper limits are compared
with the model predictions and the implications of our results for future
observations are discussed.Comment: 15 pages, 2 figures, Accepted for publication in Ap
Clinical heterogeneity can hamper the diagnosis of patients with ZAP70 deficiency
One of the severe combined immunodeficiencies (SCIDs), which is caused by a genetic defect in the signal transduction pathways involved in T-cell activation, is the ZAP70 deficiency. Mutations in ZAP70 lead to both abnormal thymic development and defective T-cell receptor (TCR) signaling of peripheral T-cells. In contrast to the lymphopenia in most SCID patients, ZAP70-deficient patients have lymphocytosis, despite the selective absence of CD8+ T-cells. The clinical presentation is usually before 2 years of age with typical findings of SCID. Here, we present three new ZAP70-deficient patients who vary in their clinical presentation. One of the ZAP70-deficient patients presented as a classical SCID, the second patient presented as a healthy looking wheezy infant, whereas the third patient came to clinical attention for the eczematous skin lesions simulating atopic dermatitis with eosinophilia and elevated immunoglobulin E (IgE), similar to the Omenn syndrome. This study illustrates that awareness of the clinical heterogeneity of ZAP70 deficiency is of utmost importance for making a fast and accurate diagnosis, which will contribute to the improvement of the adequate treatment of this severe immunodeficiency
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