Vibrational Relaxation and Redistribution Dynamics in Ruthenium(II) Polypyridyl-Based Charge-Transfer Excited States: A Combined Ultrafast Electronic and Infrared Absorption Study

Ultrafast time-resolved electronic and infrared absorption measurements have been carried out on a series of Ru­(II) polypyridyl complexes in an effort to delineate the dynamics of vibrational relaxation in this class of charge transfer chromophores. Time-dependent density functional theory calculations performed on compounds of the form [Ru­(CN-Me-bpy)x(bpy)3‑x]2+ (x = 1–3 for compounds 1–3, respectively, where CN-Me-bpy is 4,4′-dicyano-5,5′-dimethyl-2,2′-bipyridine and bpy is 2,2′-bipyridine) reveal features in their charge-transfer absorption envelopes that allow for selective excitation of the Ru­(II)–(CN-Me-bpy) moiety, the lowest-energy MLCT state(s) in each compound of the series. Changes in band shape and amplitude of the time-resolved differential electronic absorption data are ascribed to vibrational cooling in the CN-Me-bpy-localized 3MLCT state with a time constant of 8 ± 3 ps in all three compounds. This conclusion was corroborated by picosecond time-resolved infrared absorption measurements; sharpening of the CN stretch in the 3MLCT excited state was observed with a time constant of 3.0 ± 1.5 ps in all three members of the series. Electronic absorption data acquired at higher temporal resolution revealed spectral modulation over the first 2 ps occurring with a time constant of τ = 170 ± 50 fs, in compound 1; corresponding effects are significantly attenuated in compound 2 and virtually absent in compound 3. We assign this feature to intramolecular vibrational redistribution (IVR) within the 3MLCT state and represents a rare example of this process being identified from time-resolved electronic absorption data for this important class of chromophores

Mass-producible 2D-WS2 bulk modified screen printed electrodes towards the hydrogen evolution reaction

© The Royal Society of Chemistry 2019. A screen-printable ink that contained varying percentage mass incorporations of two dimensional tungsten disulphide (2D-WS2) was produced and utilized to fabricate bespoke printed electrodes (2D-WS2-SPEs). These WS2-SPEs were then rigorously tested towards the Hydrogen Evolution Reaction (HER) within an acidic media. The mass incorporation of 2D-WS2 into the 2D-WS2-SPEs was found to critically affect the observed HER catalysis with the larger mass incorporations resulting in more beneficial catalysis. The optimal (largest possible mass of 2D-WS2 incorporation) was the 2D-WS2-SPE40%, which displayed a HER onset potential, Tafel slope value and Turn over Frequency (ToF) of -214 mV (vs. RHE), 51.1 mV dec-1 and 2.20, respectively. These values significantly exceeded the HER catalysis of a bare/unmodified SPE, which had a HER onset and Tafel slope value of -459 mV (vs. RHE) and 118 mV dec-1, respectively. Clearly, indicating a strong electrocatalytic response from the 2D-WS2-SPEs. An investigation of the signal stability of the 2D-WS2-SPEs was conducted by performing 1000 repeat cyclic voltammograms (CVs) using a 2D-WS2-SPE10% as a representative example. The 2D-WS2-SPE10% displayed remarkable stability with no variance in the HER onset potential of ca. -268 mV (vs. RHE) and a 44.4% increase in the achievable current over the duration of the 1000 CVs. The technique utilized to fabricate these 2D-WS2-SPEs can be implemented for a plethora of different materials in order to produce large numbers of uniform and highly reproducible electrodes with bespoke electrochemical signal outputs

Multinational tagging efforts illustrate regional scale of distribution and threats for east pacific green turtles (Chelonia mydas agassizii).

Published onlineJournal ArticleResearch Support, Non-U.S. Gov'tThis is the final version of the article. Available from Public Library of Science via the DOI in this record.To further describe movement patterns and distribution of East Pacific green turtles (Chelonia mydas agassizii) and to determine threat levels for this species within the Eastern Pacific. In order to do this we combined published data from existing flipper tagging and early satellite tracking studies with data from an additional 12 satellite tracked green turtles (1996-2006). Three of these were tracked from their foraging grounds in the Gulf of California along the east coast of the Baja California peninsula to their breeding grounds in Michoacán (1337-2928 km). In addition, three post-nesting females were satellite tracked from Colola beach, Michoacán to their foraging grounds in southern Mexico and Central America (941.3-3020 km). A further six turtles were tracked in the Gulf of California within their foraging grounds giving insights into the scale of ranging behaviour. Turtles undertaking long-distance migrations showed a tendency to follow the coastline. Turtles tracked within foraging grounds showed that foraging individuals typically ranged up to 691.6 km (maximum) from release site location. Additionally, we carried out threat analysis (using the cumulative global human impact in the Eastern Pacific) clustering pre-existing satellite tracking studies from Galapagos, Costa Rica, and data obtained from this study; this indicated that turtles foraging and nesting in Central American waters are subject to the highest anthropogenic impact. Considering that turtles from all three rookeries were found to migrate towards Central America, it is highly important to implement conservation plans in Central American coastal areas to ensure the survival of the remaining green turtles in the Eastern Pacific. Finally, by combining satellite tracking data from this and previous studies, and data of tag returns we created the best available distributional patterns for this particular sea turtle species, which emphasized that conservation measures in key areas may have positive consequences on a regional scale.The work was supported by Earthwatch Institute, David and Lucile Packard Foundation, Wallace Research Foundation, PADI Foundation and the Arizona-Sonora Desert Museum. C. E. H. received a Masters degree bursary from the University of Exeter and the European Social Fund and would like to thank Consejo Nacional de Ciencia y Tecnología (Mexico) for support through a PhD scholarship. W. J. N. was supported by a Fulbright Fellowship and a Marshall Fellowship during the period field research in Baja California was conducted. B. J. G. is supported by the Darwin Initiative, European Social Fund and The Natural Environment Research Council. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

A peptide mimic of the chemotaxis inhibitory protein of Staphylococcus aureus: towards the development of novel anti-inflammatory compounds

Complement factor C5a is one of the most powerful pro-inflammatory agents involved in recruitment of leukocytes, activation of phagocytes and other inflammatory responses. C5a triggers inflammatory responses by binding to its G-protein-coupled C5a-receptor (C5aR). Excessive or erroneous activation of the C5aR has been implicated in numerous inflammatory diseases. The C5aR is therefore a key target in the development of specific anti-inflammatory compounds. A very potent natural inhibitor of the C5aR is the 121-residue chemotaxis inhibitory protein of Staphylococcus aureus (CHIPS). Although CHIPS effectively blocks C5aR activation by binding tightly to its extra-cellular N terminus, it is not suitable as a potential anti-inflammatory drug due to its immunogenic properties. As a first step in the development of an improved CHIPS mimic, we designed and synthesized a substantially shorter 50-residue adapted peptide, designated CHOPS. This peptide included all residues important for receptor binding as based on the recent structure of CHIPS in complex with the C5aR N terminus. Using isothermal titration calorimetry we demonstrate that CHOPS has micromolar affinity for a model peptide comprising residues 7–28 of the C5aR N terminus including two O-sulfated tyrosine residues at positions 11 and 14. CD and NMR spectroscopy showed that CHOPS is unstructured free in solution. Upon addition of the doubly sulfated model peptide, however, the NMR and CD spectra reveal the formation of structural elements in CHOPS reminiscent of native CHIPS

Litterfall, litter decomposition and associated nutrient fluxes in Pinus halepensis: influence of tree removal intensity in a Mediterranean forest

The online version of this article (doi:10.1007/s10342-015-0893-z) contains supplementary material, which is available to authorized users[EN] Our knowledge about the influence of silvicultural treatments on nutrient cycling processes in Mediterranean forests is still limited. Four levels of tree removal were compared in an Aleppo pine forest in eastern Spain to determine the effects on litterfall, litter decomposition and the associated nutrient fluxes after 12 years. Removal treatments included clearfelling, two shelterwood intensities (60 and 75 % of basal area removed) and untreated controls. Twelve years later, the basal area removed still explained 60 % of litterfall mass variance and 60 % of C, 52 % of N, 45 % of P, 17 % of K, 47 % of Ca and 60 % of Mg return variances. Litter decomposed somewhat more slowly in clearfellings compared to controls (p = 0.049), accumulated more Ca and released less K compared to the other three treatments. This was explained by contamination with mineral particles due to the poorly developed O horizon in clearfellings. We conclude that the management practices reduced the nutrient return via litterfall, but the nutrient release through decomposition seems poorly sensitive to canopy disturbance. In order to accurately quantify the harvesting impacts on nutrient cycling in this Mediterranean forest system, it is necessary to measure the litterfall of the understory layer.This work has been supported by a fellowship from the Generalitat Valenciana, Conselleria de Educacion, Formacion y Empleo awarded to L. Lado-Monserrat (BFPI/2008/041). Silvicultural treatments were carried out by the Mediterranean Centre for Environmental Studies (CEAM) through programme "I + D en relacion con la restauracion de la cubierta vegetal y otros aspectos de investigacion forestal". Dataloggers and probes were provided by the Generalitat Valenciana through Project "Efecto de diferentes sistemas de aclareo de masa forestal sobre la disponibilidad de agua, nutrientes y la regeneracion de la masa arborea y arbustiva en parcelas de pinar" (GV06/126). We acknowledge Joana Oliver, Ruth M. Tavera and Daniel Fortanet for their help in the laboratory and in the field. The authors wish to thank Francisco Galiana for his assistance, including help in fieldwork and providing information about the experimental design of the silvicultural treatments. Thanks also go to Rafael Herrera from the Centro de Ecologia, Instituto Venezolano de Investigaciones Cientificas, Caracas, Venezuela and two anonymous reviewers for critically reviewing the manuscript.Lado Monserrat, L.; Lidón, A.; Bautista, I. (2015). Litterfall, litter decomposition and associated nutrient fluxes in Pinus halepensis: influence of tree removal intensity in a Mediterranean forest. European Journal of Forest Research. 134(5):833-844. https://doi.org/10.1007/s10342-015-0893-zS8338441345Almagro M, Martínez-Mena M (2012) Exploring short-term leaf-litter decomposition dynamics in a Mediterranean ecosystem: dependence on litter type and site conditions. Plant Soil 358:323–335Alvarez A, Gracia M, Vayreda J, Retana J (2012) Patterns of fuel types and crown fire potential in Pinus halepensis forests in the Western Mediterranean Basin. For Ecol Manage 270:282–290Austin AT, Vivanco L (2006) Plant litter decomposition in a semi-arid ecosystem controlled by photodegradation. Nature 442:555–558Bates JD, Svejcar TS, Miller RF (2007) Litter decomposition in cut and uncut western juniper woodlands. J Arid Environ 70:222–236Binkley D (2008) Three key points in the design of forest experiments. For Ecol Manage 255:2022–2023Blair JM, Crossley DA Jr (1988) Litter decomposition, nitrogen dynamics and litter microarthropods in a southern Appalachian hardwood forest 8 years following clearcutting. J Appl Ecol 25:683–698Blanco JA, Zavala MA, Imbert JB, Castillo FJ (2005) Sustainability of forest management practices: evaluation through a simulation model of nutrient cycling. For Ecol Manage 213:209–228Blanco JA, Imbert JB, Castillo FJ (2006) Influence of site characteristics and thinning intensity on litterfall production in two Pinus sylvestris L. forests in the western Pyrenees. For Ecol Manage 237:342–352Blanco JA, Imbert JB, Castillo FJ (2008) Nutrient return via litterfall in two contrasting Pinus sylvestris forests in the Pyrenees under different thinning intensities. For Ecol Manage 256:1840–1852Blanco JA, Imbert JB, Castillo FJ (2011) Thinning affects Pinus sylvestris needle decomposition rates and chemistry differently depending on site conditions. Biogeochemistry 106:397–414Caldentey J, Ibarra M, Hernández J (2001) Litter fluxes and decomposition in Nothofagus pumilio stands in the region of Magallanes, Chile. For Ecol Manage 148:145–157Christensen JH, Krishna Kumar K, et al. (2013) Climate phenomena and their relevance for future regional climate change. In: Stocker TF, Qin D, Plattner G-K et al (Eds.) Climate change 2013: the physical science basis. Contribution of Working Group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge University Press, Cambridge, United Kingdom and New York, NY, USACortina J, Vallejo VR (1994) Effects of clearfelling on forest floor accumulation and litter decomposition in a radiata pine plantation. For Ecol Manage 70:299–310Entry JA, Rose CL, Cromack K Jr (1991) Litter decomposition and nutrient release in ectomycorrhizal mat soils of a Douglas fir ecosystem. Soil Biol Biochem 23:285–290Fabbio G, Merlo M, Tosi V (2003) Silvicultural management in maintaining biodiversity and resistance of forests in Europe—the Mediterranean region. J Environ Manage 67:67–76Galiana F, Pérez-Badía R, Camarero E, Estruch V, Currás R (2001) Estimación de la Radiación solar incidente en pinares de Pinus halepensis sometidos a tratamientos selvícolas de cortas finales. In: Junta de Andalucía. Consejería de Medio Ambiente (Ed.) Actas del III Congreso Forestal Español. Junta de Andalucía. Granada (Original in Spanish)García-Plé C, Vanrell P, Morey M (1995) Litter fall and decomposition in a Pinus halepensis forest on Mallorca. J Veg Sci 6:17–22González Utrillas N, González Pérez E, Galiana F (2005) Variación del crecimiento diametral de la masa de pinar de carrasco en cortas finales experimentales, en los montes de Tuejar y Chelva (Valencia). IV Congreso Forestal Español. Zaragoza. Soc. Esp. Cien. For. (Original in Spanish)Guo LB, Sims REH (1999) Litter decomposition and nutrient release via litter decomposition in New Zealand eucalypt short rotation forests. Agric Ecosyst Environ 75:133–140GVA (1995) Mapa de Suelos de la Comunidad Valenciana. Chelva (666). Proyecto LUCDEME (Icona), Centro de Investigaciones sobre Desertificación y Conselleria d’Agricultura i Mig Ambient. Generalitat Valenciana. Valencia, Spain. (Original in Spanish)Hennessey TC, Dougherty PM, Cregg BM, Wittwer RF (1992) Annual variation in needle fall of a loblolly pine stand in relation to climate and stand density. For Ecol Manage 51:329–338Inagaki Y, Kuramoto S, Torii A, Shinomiya Y, Fukata H (2008) Effects of thinning on leaf-fall and leaf-litter nitrogen concentration in hinoki cypress (Chamaecyparis obtusa Endlicher) plantation stands in Japan. For Ecol Manage 255:1859–1867Jonard M, Misson L, Ponette Q (2006) Long-term thinning effects on the forest floor and the foliar nutrient status of Norway spruce stands in the Belgian Ardennes. Can J For Res 36:2684–2695Kim C, Sharik TL, Jurgensen MF (1996a) Canopy cover effects on mass loss, and nitrogen and phosphorus dynamics from decomposing litter in oak and pine stands in northern Lower Michigan. For Ecol Manage 80:13–20Kim C, Sharik TL, Jurgensen MF (1996b) Litterfall, nitrogen and phosphorus inputs at various levels of canopy removal in oak and pine stands in northern lower Michigan. Am Midl Nat 135:195–204Kim C, Son Y, Lee WK, Jeong J, Noh NJ, Kim SR, Yang AR, Ju NG (2012) Influence of forest tending (Soopkakkugi) works on litterfall and nutrient inputs in a Pinus densiflora stand. For Sci Technol 8:83–88Kimmins JP (2004) Forest ecology, a foundation for sustainable management and environmental ethics in forestry. Prentice-Hall, New JerseyKimmins JP, Mailly D, Seely B (1999) Modelling forest ecosystem net primary production: the hybrid simulation approach used in FORECAST. Ecol Modell 122:195–224Klemmedson JO, Meier CE, Campbell RE (1990) Litter fall transfers of dry matter and nutrients in ponderosa pine stands. Can J For Res 20:1105–1115Kunhamu TK, Kumar BM, Viswanath S (2009) Does thinning affect litterfall, litter decomposition, and associated nutrient release in Acacia mangium stands of Kerala in peninsular India? Can J For Res 39:792–801Lytle DE, Cronan CS (1998) Comparative soil CO2 evolution, litter decay, and root dynamics in clearcut and uncut spruce–fir forest. For Ecol Manage 103:121–128Molina AJ, Del Campo AD (2012) The effects of experimental thinning on throughfall and stemflow: a contribution towards hydrology-oriented silviculture in Aleppo pine plantations. For Ecol Manage 269:206–213Navarro FB, Romero-Freire A, Del Castillo T, Foronda A, Jiménez MN, Ripoll MA, Sánchez-Miranda A, Hutsinger L, Fernández-Ondoño E (2013) Effects of thinning on litterfall were found after years in a Pinus halepensis afforestation area at tree and stand levels. For Ecol Manage 289:354–362Olson JS (1963) Energy storage and the balance of producers and decomposers in ecological systems. Ecology 44:322–331Pérez Cueva AJ (1994) Atlas Climático de la Comunidad Valenciana. Colección Territori nº 4. Generalitat Valenciana. Conselleria d’Obres Publiques, Urbanisme i Transport, ValenciaPetritsch R, Hasenauer H, Pietsch SA (2007) Incorporating forest growth response to thinning within biome-BGC. For Ecol Manage 242:324–336Prescott CE (1997) Effects of clearcutting and alternative silvicultural systems on rates of decomposition and nitrogen mineralization in a coastal montane coniferous forest. For Ecol Manage 95:253–260Prescott CE (2002) The influence of the forest canopy on nutrient cycling. Tree Physiol 22:1193–1200Prescott CE, Blevins LL, Staley CL (2000) Effects of clear-cutting on decomposition rates of litter and forest floor in forests of British Columbia. Can J For Res 30:1751–1757Roig S, Del Río M, Cañellas I, Montero G (2005) Litter fall in Mediterranean Pinus pinaster Ait. stands under different thinning regimes. For Ecol Manage 206:179–190Sardans J, Peñuelas J, Rodà F (2005) Changes in nutrient use efficiency, status and retranslocation in young post-fire regeneration Pinus halepensis in response to sudden N and P input, irrigation and removal of competing vegetation. Trees 19:233–250Scarascia-Mugnozza G, Oswald H, Piussi P, Radoglou K (2000) Forests of the Mediterranean region: gaps in knowledge and research needs. For Ecol Manage 132:97–109Slovik S (1997) Tree physiology. In: Hüttl RF, Schaaf W (eds) Magnesium deficiency in forest ecosystems. Kluwer Academic Publishers, London, pp 101–214Taylor BR, Parkinson D (1988) Does repeated freezing and thawing accelerate decay of leaf litter? Soil Biol Biochem 20:657–665Torras O, Saura S (2008) Effects of silvicultural treatments on forest biodiversity indicators in the Mediterranean. For Ecol Manage 255:3322–3330Trofymow JA, Barclay HJ, McCullough KM (1991) Annual rates and elemental concentrations of litter fall in thinned and fertilized Douglas-fir. Can J For Res 21:1601–1615Wallace ES, Freedman B (1986) Forest floor dynamics in a chronosequence of hardwood stands in central Nova Scotia. Can J For Res 16:293–302Whitford WG, Meentemeyer V, Seastedt TR, Cromack Jr K, Crossley Jr DA, Santos P, Todd RL, Waide JB (1981) Exceptions to the AET model: deserts and clear-cut forest. Ecology 62:275–277Yin X, Perry JA, Dixon RK (1989) Influence of canopy removal on oak forest floor decomposition. Can J For Res 19:204–21

Comparative algological and bacteriological examinations on biofilms developed on different substrata in a shallow soda lake

According to the European Water Framework Directives, benthic diatoms of lakes are a tool for ecological status assessment. In this study, we followed an integrative sample analysis approach, in order to find an appropriate substratum for the water qualification-oriented biomonitoring of a shallow soda lake, Lake Velencei. Six types of substrata (five artificial and one natural), i.e., andesite, granite, polycarbonate, old reed stems, Plexiglass discs and green reed, were sampled in May and in November. We analysed total alga and diatom composition, chlorophyll a content of the periphyton, surface tension and roughness of the substrata and carbon source utilisation of microbial communities. Water quality index was calculated based on diatom composition. Moreover, using a novel statistical tool, a self-organising map, we related algal composition to substratum types. Biofilms on plastic substrates deviated to a great extent from the stone and reed substrata, with regard to the parameters measured, whereas the biofilms developing on reed and stone substrata were quite similar. We conclude that for water quality monitoring purposes, sampling from green reed during springtime is not recommended, since this is the colonization time of periphyton on the newly growing reed, but it may be appropriate from the second half of the vegetation period. Stone and artificially placed old reed substrata may be appropriate for biomonitoring of shallow soda lakes in both spring and autumn since they showed in both seasons similar results regarding all measured features

Maternal melatonin: Effective intervention against developmental programming of cardiovascular dysfunction in adult offspring of complicated pregnancy

Funder: British Heart Foundation; Id: http://dx.doi.org/10.13039/501100000274Abstract: Adopting an integrative approach, by combining studies of cardiovascular function with those at cellular and molecular levels, this study investigated whether maternal treatment with melatonin protects against programmed cardiovascular dysfunction in the offspring using an established rodent model of hypoxic pregnancy. Wistar rats were divided into normoxic (N) or hypoxic (H, 10% O2) pregnancy ± melatonin (M) treatment (5 μg·ml−1.day−1) in the maternal drinking water. Hypoxia ± melatonin treatment was from day 15–20 of gestation (term is ca. 22 days). To control for possible effects of maternal hypoxia‐induced reductions in maternal food intake, additional dams underwent pregnancy under normoxic conditions but were pair‐fed (PF) to the daily amount consumed by hypoxic dams from day 15 of gestation. In one cohort of animals from each experimental group (N, NM, H, HM, PF, PFM), measurements were made at the end of gestation. In another, following delivery of the offspring, investigations were made at adulthood. In both fetal and adult offspring, fixed aorta and hearts were studied stereologically and frozen hearts were processed for molecular studies. In adult offspring, mesenteric vessels were isolated and vascular reactivity determined by in‐vitro wire myography. Melatonin treatment during normoxic, hypoxic or pair‐fed pregnancy elevated circulating plasma melatonin in the pregnant dam and fetus. Relative to normoxic pregnancy, hypoxic pregnancy increased fetal haematocrit, promoted asymmetric fetal growth restriction and resulted in accelerated postnatal catch‐up growth. Whilst fetal offspring of hypoxic pregnancy showed aortic wall thickening, adult offspring of hypoxic pregnancy showed dilated cardiomyopathy. Similarly, whilst cardiac protein expression of eNOS was downregulated in the fetal heart, eNOS protein expression was elevated in the heart of adult offspring of hypoxic pregnancy. Adult offspring of hypoxic pregnancy further showed enhanced mesenteric vasoconstrictor reactivity to phenylephrine and the thromboxane mimetic U46619. The effects of hypoxic pregnancy on cardiovascular remodelling and function in the fetal and adult offspring were independent of hypoxia‐induced reductions in maternal food intake. Conversely, the effects of hypoxic pregnancy on fetal and postanal growth were similar in pair‐fed pregnancies. Whilst maternal treatment of normoxic or pair‐fed pregnancies with melatonin on the offspring cardiovascular system was unremarkable, treatment of hypoxic pregnancies with melatonin in doses lower than those recommended for overcoming jet lag in humans enhanced fetal cardiac eNOS expression and prevented all alterations in cardiovascular structure and function in fetal and adult offspring. Therefore, the data support that melatonin is a potential therapeutic target for clinical intervention against developmental origins of cardiovascular dysfunction in pregnancy complicated by chronic fetal hypoxia

Personalisation in MOOCs: a critical literature review

The advent and rise of Massive Open Online Courses (MOOCs) have brought many issues to the area of educational technology. Researchers in the field have been addressing these issues such as pedagogical quality of MOOCs, high attrition rates, and sustainability of MOOCs. However, MOOCs personalisation has not been subject of the wide discussions around MOOCs. This paper presents a critical literature survey and analysis of the available literature on personalisation in MOOCs to identify the needs, the current states and efforts to personalise learning in MOOCs. The findings illustrate that there is a growing attention to personalisation to improve learners’ individual learning experiences in MOOCs. In order to implement personalised services, personalised learning path, personalised assessment and feedback, personalised forum thread and recommendation service for related learning materials or learning tasks are commonly applied

Cytotoxic T Lymphocyte Trafficking and Survival in an Augmented Fibrin Matrix Carrier

Cell-based therapies have intriguing potential for the treatment of a variety of neurological disorders. One such example is genetically engineered cytotoxic T lymphocytes (CTLs) that are being investigated in brain tumor clinical trials. The development of methods for CTL delivery is critical to their use in the laboratory and clinical setting. In our study, we determined whether CTLs can migrate through fibrin matrices and if their migration, survival, and function could be modulated by adding chemokines to the matrix. Our results indicated that CTLs can freely migrate through fibrin matrices. As expected, the addition of the monocyte chemotactic protein-1 (MCP-1), also known as chemokine C-C motif ligand 2 (CCL2), to the surrounding media increased egress of the CTLs out of the fibrin clot. Interleukin (IL) -2 and/or IL-15 embedded in the matrix enhanced T cell survival and further promoted T cell migration. The interleukin-13 receptor alpha 2 specific (IL-13R alpha2) T cells that traveled out of the fibrin clot retained the capacity to kill U251 glioma cells. In summary, CTLs can survive and migrate robustly in fibrin matrices. These processes can be influenced by modification of matrix constituents. We conclude that fibrin matrices may be suitable T cell carriers and can be used to facilitate understanding of T cell interaction with the surrounding microenvironment

Decision-making, cognitive distortions and alcohol use in adolescent problem and non-problem gamblers: an experimental study

In the psychological literature, many studies have investigated the neuropsychological and behavioral changes that occur developmentally during adolescence. These studies have consistently observed a deficit in the decision-making ability of children and adolescents. This deficit has been ascribed to incomplete brain development. The same deficit has also been observed in adult problem and pathological gamblers. However, to date, no study has examined decision-making in adolescents with and without gambling problems. Furthermore, no study has ever examined associations between problem gambling, decision-making, cognitive distortions and alcohol use in youth. To address these issues, 104 male adolescents participated in this study. They were equally divided in two groups, problem gamblers and non-problem gamblers, based on South Oaks Gambling Screen Revised for Adolescents scores. All participants performed the Iowa Gambling Task and completed the Gambling Related Cognitions Scale and the Alcohol Use Disorders Identification Test. Adolescent problem gamblers displayed impaired decision-making, reported high cognitive distortions, and had more problematic alcohol use compared to non-problem gamblers. Strong correlations between problem gambling, alcohol use, and cognitive distortions were observed. Decision-making correlated with interpretative bias. This study demonstrated that adolescent problem gamblers appear to have the same psychological profile as adult problem gamblers and that gambling involvement can negatively impact on decision-making ability that, in adolescence, is still developing. The correlations between interpretative bias and decision-making suggested that the beliefs in the ability to influence gambling outcomes may facilitate decision-making impairment