1,059 research outputs found
Image-space visibility ordering for cell projection volume rendering of unstructured data
Dendrimer Stabilized Nanoalloys for Ink-Jet Printing of Surface-Enhanced Raman Scattering Substrates
Research on paper substrates prepared by inkjet deposition of metal nanoparticles for sensing applications has become a hot topic in recent years; however, the design of such substrates based on the deposition of alloy nanoparticles remains less explored. Herein, we report for the first time the inkjet printing of dendrimer-stabilized colloidal metal nanoalloys for the preparation of paper substrates for surface-enhanced Raman scattering (SERS) spectroscopy. To this end, nanoassemblies containing variable molar ratios of Au:Ag were prepared in the presence of poly(amidoamine) dendrimer (PAMAM), resulting in plasmonic properties that depend on the chemical composition of the final materials. The dendrimer-stabilized Au:Ag:PAMAM colloids exhibit high colloidal stability, making them suitable for the preparation of inks for long-term use in inkjet printing of paper substrates. Moreover, the pre-treatment of paper with a polystyrene (PS) aqueous emulsion resulted in hydrophobic substrates with improved SERS sensitivity, as illustrated in the analytical detection of tetramethylthiuram disulfide (thiram pesticide) dissolved in aqueous solutions. We suggest that the interactions established between the two polymers (PAMAM and PS) in an interface region over the cellulosic fibres, resulted in more exposed metallic surfaces for the adsorption of the analyte molecules. The resulting hydrophobic substrates show long-term plasmonic stability with high SERS signal retention for at least ninety days.publishe
Partial characterization and anticoagulant activity of a heterofucan from the brown seaweed Padina gymnospora
The brown algae Padina gymnospora contain different fucans. Powdered algae were submitted to proteolysis with the proteolytic enzyme maxataze. The first extract of the algae was constituted of polysaccharides contaminated with lipids, phenols, etc. Fractionation of the fucans with increasing concentrations of acetone produced fractions with different proportions of fucose, xylose, uronic acid, galactose, and sulfate. One of the fractions, precipitated with 50% acetone (v/v), contained an 18-kDa heterofucan (PF1), which was further purified by gel-permeation chromatography on Sephadex G-75 using 0.2 M acetic acid as eluent and characterized by agarose gel electrophoresis in 0.05 M 1,3 diaminopropane/acetate buffer at pH 9.0, methylation and nuclear magnetic resonance spectroscopy. Structural analysis indicates that this fucan has a central core consisting mainly of 3-ß-D-glucuronic acid 1-> or 4-ß-D-glucuronic acid 1 ->, substituted at C-2 with alpha-L-fucose or ß-D-xylose. Sulfate groups were only detected at C-3 of 4-alpha-L-fucose 1-> units. The anticoagulant activity of the PF1 (only 2.5-fold lesser than low molecular weight heparin) estimated by activated partial thromboplastin time was completely abolished upon desulfation by solvolysis in dimethyl sulfoxide, indicating that 3-O-sulfation at C-3 of 4-alpha-L-fucose 1-> units is responsible for the anticoagulant activity of the polymer.Universidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de GlicobiologiaUniversidade Federal do Rio Grande do Norte Departamento de Bioquímica Laboratório de Biotecnologia de Polímeros NaturaisUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de BioquímicaUNIFESP, EPM, Depto. de BioquímicaSciEL
Exploring Relationships Between Anthropometry, Body Composition, Maturation, and Selection for Competition:A Study in Youth Soccer Players
Purpose: The purpose of this study was to analyze variations of selection for competition between late and early mature players and test the relationships between anthropometry, body composition, maturation, and selection for competition.
Methods: Seventy-nine youth soccer players from under-11 to under-14 participated in this study, over 6 months. Body composition and maturity offset were estimated based on anthropometric data collected. Participants were also monitored for their number of matches as starters and time of play accrued in minutes.
Results: Minutes played had large correlation coefficients with maturity offset (r = 0.58), and leg length and sitting height interaction (r = 0.56). Multiple linear regression explained 35% of the variation in minutes played (p < 0.001, R2 = 0.41, R2adjusted = 0.35, RMSE = 334.8), but only 12% of the variation in matches as starter (p = 0.04, R2 = 0.21, R2adjusted = 0.12, RMSE = 5.47) between above and below the median of the maturity offset was accounted for, respectively.
Conclusions: Although maturation may play a role in the minutes of play accrued and matches as starters in young, it is not necessarily determining. A significant amount of the variation in the minutes of play accrued of players can be accounted for when considering body composition and anthropometric data
Associations between movement behaviours and obesity markers among preschoolers compliant and non-compliant with sleep duration:A latent profile analysis
This study identifies physical activity (PA) and sedentary behaviour (SB) clusters in preschoolers compliant (C) or non-compliant (NC) with sleep recommendations; and associates these clusters with obesity markers. PA and SB were objectively assessed (Actigraph WGT3-X) in 272 preschoolers (4.4 ± 0.7 years old). Sleep duration was parent-reported, and preschoolers were classified as C (3–4 years old: 600–780 min/day; 5 years old: 540–660 min/day) or NC with sleep recommendations. Body mass index (BMI) and waist circumference (WC) were assessed according to international protocols. Moderate to vigorous physical activity (MVPA) and light physical activity (LPA) were categorized as low/high (60 min/day or <180 min/180 min/day, respectively). SB was defined according to mean values between clusters. Latent profile analysis was performed. Associations between the observed clusters and obesity markers were determined using linear regression (RStudio; 1.3.1073). Four cluster solutions for C and NC preschoolers were identified. A negative association between C/Low MVPA cluster and BMI, and a positive association between NC/Low MVPA and BMI (β = −0.8, 95%CI = −1.6;−0.1, and β = 0.9, 95%CI = 0.1;1.7, respectively) were observed. No association was seen for SB clusters. Adequate sleep duration may have a protective role for preschoolers’ BMI, even if the children do not comply with MVPA recommendations
Heritabilities, proportions of heritabilities explained by GWAS findings, and implications of cross-phenotype effects on PR interval
Electrocardiogram (ECG) measurements are a powerful tool for evaluating cardiac function and are widely used for the diagnosis and prediction of a variety of conditions, including myocardial infarction, cardiac arrhythmias, and sudden cardiac death. Recently, genome-wide association studies (GWASs) identified a large number of genes related to ECG parameter variability, specifically for the QT, QRS, and PR intervals. The aims of this study were to establish the heritability of ECG traits, including indices of left ventricular hypertrophy, and to directly assess the proportion of those heritabilities explained by GWAS variants. These analyses were conducted in a large, Dutch family-based cohort study, the Erasmus Rucphen Family study using variance component methods implemented in the SOLAR (Sequential Oligogenic Linkage Analysis Routines) software package. Heritability estimates ranged from 34 % for QRS and Cornell voltage product to 49 % for 12-lead sum. Trait-specific GWAS findings for each trait explained a fraction of their heritability (17 % for QRS, 4 % for QT, 2 % for PR, 3 % for Sokolow–Lyon index, and 4 % for 12-lead sum). The inclusion of all ECG-associated single nucleotide polymorphisms explained an additional 6 % of the heritability of PR. In conclusion, this study shows that, although GWAS explain a portion of ECG trait variability, a large amount of heritability remains to be explained. In addition, larger GWAS for PR are likely to detect loci already identified, particularly those observed for QRS and 12-lead sum
Entropy and Spin Susceptibility of s-wave Type-II Superconductors near
A theoretical study is performed on the entropy and the spin
susceptibility near the upper critical field of s-wave
type-II superconductors with arbitrary impurity concentrations. The changes of
these quantities through may be expressed as , for example, where is the average flux density
and denotes entropy in the normal state. It is found that the
slopes and at T=0 are identical, connected
directly with the zero-energy density of states, and vary from 1.72 in the
dirty limit to in the clean limit. This mean-free-path dependence
of and at T=0 is quantitatively the same as that
of the slope for the flux-flow resistivity studied
previously. The result suggests that and near
T=0 are convex downward (upward) in the dirty (clean) limit, deviating
substantially from the linear behavior . The specific-heat
jump at also shows fairly large mean-free-path dependence.Comment: 8 pages, 5 figure
Hypothalamic S1p/s1pr1 axis controls energy homeostasis
Sphingosine 1-phosphate receptor 1 (S1PR1) is a G-protein-coupled receptor for sphingosine-1-phosphate (S1P) that has a role in many physiological and pathophysiological processes. Here we show that the S1P/S1PR1 signalling pathway in hypothalamic neurons regulates energy homeostasis in rodents. We demonstrate that S1PR1 protein is highly enriched in hypothalamic POMC neurons of rats. Intracerebroventricular injections of the bioactive lipid, S1P, reduce food consumption and increase rat energy expenditure through persistent activation of STAT3 and the melanocortin system. Similarly, the selective disruption of hypothalamic S1PR1 increases food intake and reduces the respiratory exchange ratio. We further show that STAT3 controls S1PR1 expression in neurons via a positive feedback mechanism. Interestingly, several models of obesity and cancer anorexia display an imbalance of hypothalamic S1P/S1PR1/STAT3 axis, whereas pharmacological intervention ameliorates these phenotypes. Taken together, our data demonstrate that the neuronal S1P/S1PR1/STAT3 signalling axis plays a critical role in the control of energy homeostasis in rats.Sphingosine 1-phosphate receptor 1 (S1PR1) is a G-protein-coupled receptor for sphingosine-1-phosphate (S1P) that has a role in many physiological and pathophysiological processes. Here we show that the S1P/S1PR1 signalling pathway in hypothalamic neurons regulates energy homeostasis in rodents. We demonstrate that S1PR1 protein is highly enriched in hypothalamic POMC neurons of rats. Intracerebroventricular injections of the bioactive lipid, S1P, reduce food consumption and increase rat energy expenditure through persistent activation of STAT3 and the melanocortin system. Similarly, the selective disruption of hypothalamic S1PR1 increases food intake and reduces the respiratory exchange ratio. We further show that STAT3 controls S1PR1 expression in neurons via a positive feedback mechanism. Interestingly, several models of obesity and cancer anorexia display an imbalance of hypothalamic S1P/S1PR1/STAT3 axis, whereas pharmacological intervention ameliorates these phenotypes. Taken together, our data demonstrate that the neuronal S1P/S1PR1/STAT3 signalling axis plays a critical role in the control of energy homeostasis in rats5485
Discrete symmetries, invisible axion and lepton number symmetry in an economic 3-3-1 model
We show that Peccei-Quinn and lepton number symmetries can be a natural
outcome in a 3-3-1 model with right-handed neutrinos after imposing a Z_11 x
Z_2 symmetry. This symmetry is suitably accommodated in this model when we
augmented its spectrum by including merely one singlet scalar field. We work
out the breaking of the Peccei-Quinn symmetry, yielding the axion, and study
the phenomenological consequences. The main result of this work is that the
solution to the strong CP problem can be implemented in a natural way, implying
an invisible axion phenomenologically unconstrained, free of domain wall
formation and constituting a good candidate for the cold dark matter.Comment: 17 pages, Revtex
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