551 research outputs found

    Sustainability assessment of building rehabilitation actions in old urban centres

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    It is essential that the society understands the importance of sustainable development, its implication for the existing living standards and the need to implement management policies that promote sustainability. Rehabilitation assumes a fundamental role in this process; however, we are faced with the lack of specific, adequate and simplified tools for the sustainability assessment in rehabilitation actions. In this paper, the analysis of different sustainability assessment systems was conducted, comparing them in order to identify the adjustments to be made to a Portuguese model taken under consideration. Subsequently, the model was cross-checked with current European and national urban rehabilitation and regeneration policies, analysing their contribution to sustainability, in order to define the assessment criteria and create a Simplified Method for the Sustainability Assessment for rehabilitation in old urban centres, which can be a useful tool, easy to use by all actors in the process and as a way of preserving the history of this areas, and the memory of those who inhabit them. This Method considers five major areas: Water, Energy, Materials, Emissions and Cultural, Economic and Social Environments. For each of these criteria, measurement indicators and levels of assessment were settled, adjusted to the context of old urban centres.info:eu-repo/semantics/publishedVersio

    Multiplicity distributions inside parton cascades developing in a medium

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    The explanation of the suppression of high-pT hadron yields at RHIC in terms of jet-quenching implies that the multiplicity distributions of particles inside a jet and jet-like particle correlations differ strongly in nucleus-nucleus collisions at RHIC or at the LHC from those observed at e+e- or hadron colliders. We present a framework for describing the medium-induced modification, which has a direct interpretation in terms of a probabilistic medium-modified parton cascade, and which treats leading and subleading partons on an equal footing. We show that our approach can account for the strong suppression of single inclusive hadron spectra measured in Au-Au collisions at RHIC, and that this implies a characteristic distortion of the single inclusive distribution of soft partons inside the jet. We determine, as a function of the jet energy, to what extent the soft fragments within a jet can be measured above some momentum cut.Comment: 5 pages, 4 eps-figures; talk given at Hot Quarks 2006, Villasimius (Sardinia, Italy), May 15-20, 200

    Influence of substitutional disorder on the electrical transport and the superconducting properties of Fe1+z_{1+z}Te1−x−y_{1-x-y}Sex_{x}Sy_{y}

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    We have carried out an investigation of the structural, magnetic, transport and superconducting properties of Fe1+z_{1+z}Te1−x−y_{1-x-y}Sex_xSy_y ceramic compounds, for z=0z=0 and some specific Se (0≀\leq x ≀\leq 0.5) and S (0 ≀\leq y ≀\leq0.12) contents. The incorporation of Se and S to the FeTe structure produces a progressive reduction of the crystallographic parameters as well as different degrees of structural disorder associated with the differences of the ionic radius of the substituting cations. In the present study, we measure transport properties of this family of compounds and we show the direct influence of disorder in the normal and superconductor states. We notice that the structural disorder correlates with a variable range hopping conducting regime observed at temperatures T>T > 200 K. At lower temperatures, all the samples except the one with the highest degree of disorder show a crossover to a metallic-like regime, probably related to the transport of resilient-quasi-particles associated with the proximity of a Fermi liquid state at temperatures below the superconducting transition. Moreover, the superconducting properties are depressed only for that particular sample, in accordance to the condition that superconductivity is affected by disorder when the electronic localization length ΟL\xi_L becomes smaller than the coherence length ΟSC\xi_{SC}.Comment: 23 pages, 9 figure

    Analysis Of The Ergosterol Biosynthesis Pathway Cloning, Molecular Characterization And Phylogeny Of Lanosterol 14 α-demethylase (erg11) Gene Of Moniliophthora Perniciosa

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    The phytopathogenic fungus Moniliophthora perniciosa (Stahel) Aime & Philips-Mora, causal agent of witches’ broom disease of cocoa, causes countless damage to cocoa production in Brazil. Molecular studies have attempted to identify genes that play important roles in fungal survival and virulence. In this study, sequences deposited in the M. perniciosa Genome Sequencing Project database were analyzed to identify potential biological targets. For the first time, the ergosterol biosynthetic pathway in M. perniciosa was studied and the lanosterol 14α-demethylase gene (ERG11) that encodes the main enzyme of this pathway and is a target for fungicides was cloned, characterized molecularly and its phylogeny analyzed.ERG11 genomic DNA and cDNA were characterized and sequence analysis of the ERG11 protein identified highly conserved domains typical of this enzyme, such as SRS1, SRS4, EXXR and the heme-binding region (HBR). Comparison of the protein sequences and phylogenetic analysis revealed that the M. perniciosa enzyme was most closely related to that of Coprinopsis cinerea.374683693Aime, M.C., Phillips-Mora, W., The causal agents of witches’ broom and frost pod rot of cacao (chocolate, Theobroma cacao) from a new lineage of Marasmiaceae (2005) Mycologia, 97, pp. 1012-1022Albertini, C., Thebaud, G., Fournier, E., Leroux, P., Eburicol 14α-demethylase gene (CYP51) polymorphism and speciation in Botrytis cinerea (2002) Mycol Res, 106, pp. 1171-1178Altschul, S.F., Gish, W., Miller, W., Myersewand Lipman, D.J., Basic local alignment search tool (1990) J Mol Biol, 215, pp. 403-410Bak, S., Kahn, R.A., Oisen, C.E., Halkier, B.A., Cloning and expression in Escherichia coli of the obtusifoliol 14α-demethylase of Sorghum bicolor (L.) Moench, a cytochrome P450 orthologous to the sterol 14α demethylases (CYP51) from fungi and mammals (1997) Plant J, 11, pp. 191-201Barrett-Bee, K., Dixon, G., Ergosterol biosynthesis inhibition: A target for antifungal agents (1995) Acta Biochim Pol, 42, pp. 465-480Bellamine, A., Mangla, A.T., Nes, W.D., Waterman, M.R., Characterization and catalytic properties of the sterol 14α-demethylase from Mycobacterium tuberculosis (1999) Proc Natl Acad Sci USA, 96, pp. 8937-8942Butler, G., Rasmussen, M.D., Lin, M.F., Santos, M.A., Sakthikumar, S., Munro, C.A., Rheinbay, E., Reedy, J.L., Evolution of pathogenicity and sexual reproduction in eight Candida genomes (2009) Nature, 459, pp. 657-662Carrillo-Muñoz, A.J., Giusiano, G., Ezkurra, P.A., QuindĂłs, G., Antifungal agents: Mode of action in yeast cells (2006) Rev Esp Quim, 19, pp. 130-139Ceita, G.O., Macedo, J.N., Santos, T.B., Alemanno, L., Gesteira, A.S., Micheli, F., Mariano, A.C., Meinhardt, L.W., Involvement of calcium oxalate degradation during programmed cell death in Theobroma cacao tissues triggered by the hemibiotrophic fungus Moniliophthora perniciosa (2007) Plant Sci, 173, pp. 106-117D’souza, C.A., Kronstad, J.W., Taylor, G., Warren, R., Yuen, M., Hu, G., Jung, W.H., Tangen, K., Genome variation in Cryptococcus gattii, an emerging pathogen of immunocompetent hosts (2011) MBio, 2, pp. e00342-e00410DĂ©lye, C., Laigret, F., Corio-Costet, M.F., Cloning and sequence analysis of the eburicol 14α-demethylase gene of the obligate biotrophic grape powdery mildew fungus (1997) Gene, 195, pp. 29-33Dujon, B., Sherman, D., Fischer, G., Durrens, P., Casaregola, S., Lafontaine, I., De Montigny, J., Talla, E., Genome evolution in yeasts (2004) Nature, 430, pp. 35-44Evans, H.C., Cacao diseases - The trilogy revisited (2007) Phytopathology, 97, pp. 1640-1643Felsenstein, J., Confidence limits on phylogenies: An approach using the bootstrap (1985) Evolution, 39, pp. 783-791Formighieri, E.F., Tiburcio, R.A., Armas, E.D., Medrano, 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Birch, R., Witches’ brooms and frosty pods: Two major pathogens of cacao (2003) New Zeal J Bot, 41, pp. 423-435Hall, T.A., BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT (1999) Nucleic Acids Res, 41, pp. 95-98Hof, H., Critical annotations to the use of azole antifungals for plant protection (2001) Antimicrob Agents Chemother, 45, pp. 2987-2990Jackson, C.J., Lamb, D.C., Marczylo, T.H., Parker, J.E., Manning, N.L., Kelly, D.E., Kelly, S.L., Conservation and cloning of CYP51: A sterol 14α-demethylase from Mycobacterium smegmatis (2003) Biochem Biophys Res Commun, 301, pp. 558-563James, T.Y., Kauff, F., Schoch, C.L., Matheny, P.B., Hofstetter, V., Cox, C.J., Celio, G., Miadlikowska, J., Reconstructing the early evolution of Fungi using a six-gene phylogeny (2006) Nature, 19, pp. 818-822Jones, T., Federspiel, N.A., Chibana, H., Dungan, J., Kalman, S., Magee, B.B., Newport, G., Magee, P.T., The diploid genome sequence of Candida albicans (2004) Proc Natl Acad Sci USA, 101, pp. 7329-7334Kairuz, P.B., Zuber, J.P., Jaunin, P., Buchman, T.G., Bille, J., Rossier, M., Rapid detection and identification of Candida albicans and Torulopsis (Candida) glabrata in clinical specimens by species-specific nested PCR amplification of a cytochrome P-450 lanosterol-α-demethylase (L1A1) gene fragment (1994) J Clin Microbiol, 32, pp. 1902-1907Kalb, V.F., Woods, C.W., Turi, T.G., Dey, C.R., Sutter, T.R., Loper, J.C., Primary structure of the P450 lanosterol demethylase gene from Saccharomyces cerevisiae (1987) DNA, 6, pp. 529-537Kall, L., Krogh, A., Sonnhammer, E.L., Advantages of combined transmembrane topology and signal peptide prediction- the Phobius web server (2007) Nucleic Acids Res 35:429-, p. 432Kim, D., Lim, Y.R., Ohk, S.O., Kim, B.J., Chun, Y.J., Functional expression and characterization of CYP51 from dandruffcausing Malassezia globosa (2011) FEMS Yeast Res, 11, pp. 80-87Lai, M.H., Kirsch, D.R., Nucleotide sequence of 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determinant in Monilia fructicola field isolates from Georgia (2008) Appl Environ Microb, 74, pp. 359-366Martin, F., Aerts, A., AhrĂ©n, D., Brun, A., Danchin, E.G., Duchaussoy, F., Gibon, J., Pereda, V., The genome of Laccaria bicolor provides insights into mycorrhizal symbiosis (2008) Nature, 452, pp. 88-92McQuilken, M.P., Rudgard, S.A., Sensitivity of Crinipellis periciosa to two triazole fungicides in vitro and their effect on development of the fungus in cocoa (1988) Plant Pathol, 37, pp. 499-506Meinhardt, L.W., Bellato, C.M., Rincones, J., Azevedo, R.A., Cascardo, J.C.M., Pereira, G.A.G., In vitro production of biotrophic- like cultures of Crinipellis perniciosa, the causal agent of Witches’ broom disease of Theobroma cacao (2006) Curr Microbiol, 52, pp. 191-196Mellado, E., Guerra, T.M.D., Estrela, M.C., Tudela, J.L.R., Identification of two different 14α-sterol demethylase related genes (cyp51A and cyp51B) in Aspergillus fumigatus and other Aspergillus species (2001) J Clin Microbiol 39:2431-, p. 2438Mondego, J.M.C., Carazzolle, M.F., Costa, G.G.L., Formighieri, E.F., Parizzi, L.P., Rincones, J., Cotomacci, C., Carrer, H., A genome survey of Moniliophthora perniciosa gives new insights into Witches’ broom disease of cacao (2008) BMC Genomics, 9, pp. 1-25Morales, I.J., Vohra, P.K., Puri, V., Kottom, T.J., Limper, A.H., Thomas, C.F., Characterization of a lanosterol 14α demethylase from Pneumocystis carinii (2003) Am J Resp Cell Mol, 29, pp. 232-238Mota, S.G.R., Barros, T.F., Castilho, M.S., In vitro screening and chemometrics analysis on a series of azole derivativeswith fungicide activity against Moniliophthora perniciosa (2010) J Braz Chem Soc, 21, pp. 510-519Nierman, W.C., Pain, A., Anderson, M.J., Wortman, J.R., Kim, H.S., Arroyo, J., Berriman, M., Bermejo, C., Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus (2005) Nature, 438, pp. 1151-1156Page, R.D.M., TREEVIEW: An application to display phylogenetic trees on personal computers (1996) Comput Appl Biosci, 12, pp. 357-358Park, H.G., Lee, I.S., Chun, Y.J., Yun, C.H., Johnston, J.B., Montellano, P.R.O., Kim, D., Heterologous expression and characterization of the sterol 14α-demethylase CYP51F from Candida albicans (2011) Arch Biochem Biophys, 509, pp. 9-15Pereira, J.L., Ram, A., Figueiredo, J.M., Almeida, L.C.C., Primeira ocorrĂȘncia de vassoura-de-bruxa na principal regiĂŁo produtora de cacau do Brasil (1989) AgrotrĂłpica, 1, pp. 79-81Petersen, T.N., Brunak, S., Heijne, G., Nielsen, H., SignalIP 4.0: Discriminating signal peptides from transmembrane regions (2011) Nat Methods, 10, pp. 785-786Pietila, M.P., Vohra, P.K., Sanyat, B., Wengenack, N.L., Raghavakaimal, S., Thomas, C.F., Cloning and characterization of CYP51 from Mycobacterium avium (2006) Am J Resp Cell Mol, 35, pp. 236-240Pires, A.B.L., Gramacho, K.P., Silva, D.C., GĂłes-Neto, A., Silva, M.M., Muniz-Sobrinho, J.S., Porto, R.F., Cascardo, J.C.M., Early development of Moniliophthora perniciosa basidiomata and developmentally regulated genes (2009) BMC Microbiol, 9, p. e158Purdy, L.H., Schimidt, R.A., Status of cacao witches’ broom: Biology, epidemiology, and management (1996) Annu Rev Phytopathol, 34, pp. 573-594Raeder, U., Broda, P., Rapid preparation of DNA from filamentous fungi (1985) Lett Appl Microbiol, 1, pp. 17-20Revankar, S.G., Fu, J., Rinaldi, M.G., Kelly, S.L., Kelly, D.E., Lamb, D.C., Keller, S.M., Wickes, B.L., Cloning and characterization of the lanosterol 14α-demethylase (ERG11) gene in Cryptococcus neoformans (2004) Biochem Biophys Res Commun, 324, pp. 719-728Rincones, J., Scarpari, L.M., Carazzolle, M.F., Mondego, J.M.C., Formighieri, E.F., Barau, J.G., Costa, G.G.L., Vilas-Boas, L.A., Differential gene expression between the biotrophic-like and saprotrophic mycelia of the witches’ broom pathogen Moniliophthora perniciosa (2008) Mol Plant Microbe Int, 21, pp. 891-908Rio, M.C.S., Oliveira, B.V., Tomazella, D.P.T., Silva, J.A.F., Pereira, G.A.G., Production of calcium oxalate crystals by the basidiomycete Moniliophthora perniciosa, the causal agent of witches’ broom disease of cacao (2008) Curr Microbiol, 56, pp. 363-370Rozman, D., Stromstedt, M., Tsui, L.C., Scherer, S.W., Waterman, M.R., Structure and mapping of the human lanosterol 14α-demethylase gene (CYP51) encoding the cytochrome P450 involved in cholesterol biosynthesis: Comparison of exon/intron organization with other mammalian and fungal CYP genes (1996) Genomics, 38, pp. 371-381Sheng, C., Miao, Z., Ji, H., Yao, J., Wang, W., Che, X., Dong, G., Zhang, W., Three-dimensional model of lanosterol 14α-demethylase from Cryptococcus neoformans: Active- site characterization and insights into azole binding (2009) Antimicrob Agents Chemother, 53, pp. 3487-3495Sigrist, C.J.A., Cerutti, L., Castro, E., Langendijk-Genevaux, P.S., Bulliard, V., Bairoch, A., Hulo, N., PROSITE, a protein domain database for functional characterization and annotation (2009) Nucleic Acids Res, 38, pp. 161-166Stajich, J.E., Wilke, S.K., AhrĂ©n, D., Au, C.H., Birren, B.W., Borodovsky, M., Burns, C., Cheng, C.K., Insights into evolution of multicellular fungi from the assembled chromosomes of the mushroom Coprinopsis cinerea (Coprinus cinereus) (2010) Proc Natl Acad Sci USA, 107, pp. 11889-11894Stanke, M., Keller, O., Gunduz, I., Hayes, A., Waack, S., Morgenstern, B., AUGUSTUS: Ab initio prediction of alternative transcripts (2006) Nucleic Acids Res, 34, pp. 435-439Swofford, D.L., PAUP - Phylogenetic Analysis Using Parsimony (and other methods). Version 4.0b10 (2002) Sinauer Associates, , Sunderland, MATer-Hovhannisyan, V., Lomsadze, L., Chernoff, Y.O., Borodovsky, M., Gene prediction in novel fungal genomes using an ab initio algorithm with unsupervised training (2008) Genome Res, 18, pp. 1979-1990Thompson, J.D., Higgins, D.G., Gibson, T.J., CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weigh matrix choice (1994) Nucleic Acids Res, 22, pp. 4673-4680Veen, M., Lang, C., Interactions of the ergosterol biosynthetic pathway with other lipid pathways (2005) Biochem Soc Trans, 33, pp. 1178-1181Warrilow, A.G.S., Melo, N., Martel, C.M., Parker, J.E., Nes, W.D., Kelly, S.L., Kelly, D.E., Expression, purification and characterization of Aspergillus fumigatus sterol 14α demethylase (CYP51) isoenzymes A and B (2010) Antimicrob Agents Chemother, 54, pp. 4225-4234Waterman, M.R., Lepesheva, G.I., Sterol 14 _-demethylase, an abundant and essential mixed-function oxidase (2005) Biochem Biophys Res Commun, 338, pp. 418-422Wood, H.M., Dickinson, M.J., Lucas, J.A., Dyer, P.S., Cloning of the CYP51 gene from the eyespot pathogen Tapesia yallundae indicates that resistance to the DMI fungicide prochloraz is not related to sequence changes in the gene encoding the target site enzyme (2001) FEMS Microbiol Lett, 196, pp. 183-187Zhao, L., Liu, D., Zhang, Q., Zhang, S., Wan J and XiaoW(2007) Expression and homology modeling of sterol 14α-demethylase from Penicillium digitatium FEMS Microbiol Lett, 277, pp. 37-4

    Chaotic scalar fields as models for dark energy

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    We consider stochastically quantized self-interacting scalar fields as suitable models to generate dark energy in the universe. Second quantization effects lead to new and unexpected phenomena is the self interaction strength is strong. The stochastically quantized dynamics can degenerate to a chaotic dynamics conjugated to a Bernoulli shift in fictitious time, and the right amount of vacuum energy density can be generated without fine tuning. It is numerically observed that the scalar field dynamics distinguishes fundamental parameters such as the electroweak and strong coupling constants as corresponding to local minima in the dark energy landscape. Chaotic fields can offer possible solutions to the cosmological coincidence problem, as well as to the problem of uniqueness of vacua.Comment: 30 pages, 3 figures. Replaced by final version accepted by Phys. Rev.

    Molecular characterization of dissolved organic matter linked to microbial (Bacteria and Archaea) diversity in the main water masses of the Eastern North Atlantic Ocean

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    The microbe-dissolved organic matter (DOM) interactions include microbial uptake and DOM reworking and release, affecting the composition of the heterogeneous DOM pool. In turns, this distinct DOM composition can select for microbial assemblages. We investigated the diversity of microbial (both Bacteria and Archaea) communities (combining Illumina tag sequencing of 16S rRNA gen -amplicon sequencing variants, ASVs- and metagenomics) and the chemodiversity of dissolved organic molecules (extracted by solid phase extraction and analyzed by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry, FT-ICR-MS) in the Eastern North Atlantic Ocean off the Galician coast (43oN, 9o-15oW). Sampling ranged from 100 m to 5000 m, thereby encompassing a wide variety of water masses with contrasting origins and different aging. Applying diversity ecological metrics to both organic compounds and ASVs, we found that microbial diversity and richness were negatively correlated with DOM concentration and chemo-diversity. Besides, our results demonstrated the link between this trend and water mass aging, which enhances biosphere taxonomic diversity but reduces molecular variety. DOM diversity, decreasing along the water mass aging gradient, would likely reflect the persistence of the most refractory molecules, generated as by- product of the DOM degradation by microbes. In two PCoAs based on the metagenomic data, combined PCoA axis 1 and PCoA axis 2, explained the 80% and 20% of the microbial gene ́s structural variability among water masses. The oldest water masses, originated at high latitudes, such as NADW and LDW, were associated to higher abundance of genes involved in metabolism of aromatic compound. Intermediate waters such as ENACWst were related to sulphur/iron/phosphorous metabolism related genes. Surface waters were linked to genes involved in photosynthesis, autotrophy and cell division. Taken together, the observed increase of DOM homogenization along water mass aging was associated to differences in the functional diversity of microbial communities

    Diversity of ferns and lycophytes in Brazil

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    This compilation of ferns and lycophytes in Brazil is an update of the one published in 2010 in CatĂĄlogo de Plantas e Fungos do Brasil. The methodology consisted in collecting data from regional checklists, taxonomic revisions, and selected databases. Invited specialists improved the list accessing a website housed at the Jardim BotĂąnico do Rio de Janeiro. The results show 1,253 species: 1,111 of ferns and 142 of lycophytes. This number is 6.5% higher than the previous one (1,176 spp.). The percentage of endemic species decreased from 38.2% to 36.7%. We recognized 36 families and 133 genera (vs. 33 families, 121 genera in 2010). The 10 most diverse families are Pteridaceae (196 spp.), Dryopteridaceae (179), Polypodiaceae (164), Hymenophyllaceae (90), Thelypteridaceae (86), Aspleniaceae (78), Lycopodiaceae (64), Selaginellaceae (55), Anemiaceae (51), and Cyatheaceae (45). The three most diverse genera are still Elaphoglossum (87 spp.), Thelypteris (85), and Asplenium (74). The richest phytogeographic domain continues to be in the Atlantic Rainforest with 883 species which also has the largest number of endemic and threatened species, followed by the Amazon Rainforest (503), Cerrado (269), Pantanal (30), Caatinga (26), and Pampa (eight). Minas Gerais remains as the richest state (657 spp. vs. 580 in 2010).Esta compilação de samambaias e licĂłfitas do Brasil Ă© uma atualização daquela de 2010, no CatĂĄlogo de Plantas e Fungos do Brasil. A metodologia consistiu na reuniĂŁo de dados de listas regionais, revisĂ”es de grupos e bancos de dados selecionados. Especialistas convidados melhoraram a lista atravĂ©s do acesso a um sĂ­tio da web do Jardim BotĂąnico do Rio Janeiro. Os resultados apontam uma diversidade de 1.253 espĂ©cies, sendo 1.111 samambaias e 142 licĂłfitas. Este nĂșmero Ă© 6,5% maior que o anterior (1.176 espĂ©cies). As espĂ©cies endĂȘmicas decresceram de 38,2% para 36,7%. Foram reconhecidas 36 famĂ­lias e 133 gĂȘneros (vs. 33 famĂ­lias, 121 gĂȘneros em 2010). As dez famĂ­lias mais diversas sĂŁo: Pteridaceae (196 espĂ©cies), Dryopteridaceae (179), Polypodiaceae (164), Hymenophyllaceae (90), Thelypteridaceae (86), Aspleniaceae (78), Lycopodiaceae (64), Selaginellaceae (55), Anemiaceae (51) e Cyatheaceae (45). Os trĂȘs gĂȘneros mais diversos continuam sendo Elaphoglossum (87 espĂ©cies), Thelypteris (85) e Asplenium (74). O DomĂ­nio FitogeogrĂĄfico mais rico continua sendo a Mata AtlĂąntica (883 espĂ©cies) e tambĂ©m com mais espĂ©cies endĂȘmicas e ameaçadas, seguido pela AmazĂŽnia (503 espĂ©cies), Cerrado (269), Pantanal (30), Caatinga (26) e Pampa (oito). Minas Gerais permanece como o estado com maior riqueza (657 espĂ©cies vs. 580 em 2010)

    Studies on the mineral nutrition of the rice plant: XII. effects of the deficiencies of macronutrients on the varieties IAC-25 and IAC-47

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    Plantas de arroz, variedades IAC-25 e IAC-47, foram cultivadas em solução nutritiva completa e com deficiĂȘncia de macronutrientes. Foram obtidos dados sobre a influĂȘncia dos tratamentos na ĂĄrea foliar, produção de matĂ©ria seca e na composição mineral e observados os sintomas de carĂȘncia. O estado nutricional das plantas deficientes em N foi tambĂ©m avaliado pela determinação da atividade da redĂștase de nitrato na folha.Rice plants, varieties IAC-25 and IAC-47 were grown in nutrient solution both in the presence and in the absence of macronutrients. Symptons of deficiency were observed in general agreement with description found in the literature. The deficiency of N affected most dry matter yield among the three major macronutrients. although the effect on grain production was greater when K was omitted. The following levels could be taken as indication of adequate nutrition when leaf samples are analysed at the boot stage (respectively) for var. IAC-25 and IAC-47): N - 4.18% and 3.54%; P - 0.36 and 0.27; K - 2.30 and 2.09; Ca - 1.22 and 1.00; Mg - 0.80 and 0.77; S - 0.24 and 0.18. Leaf nitrate reductase activity at the same physiological period correlated well both with N supply and dry matter production
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