Characterization of monoclonal and polyclonal antibodies to bovine enteric coronavirus: Establishment of an efficient ELISA for antigen detection in feces

Monoclonal antibodies to bovine enteric coronavirus (BEC) were produced. Additionally, polyclonal antibodies were made in rabbits and guinea pigs and extracted from the yolk of immunized hens. The antibodies were characterized by neutralization test, hemagglutination inhibition test, enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Neutralizing antibody titers of polyclonal antisera ranged from 1:1280 to 1:40 000. Only one out of 908 hybridoma colonies tested secreted antibodies with neutralizing activity. By ELISA, polyclonal sera exhibited high background reactions that could be significantly reduced by treatment with kaolin in the case of rabbit sera. Attempts to establish an ELISA for BEC antigen detection based on polyclonal sera failed due to low sensitivity and specificity. Optimal results were achieved when a mixture of two monoclonal antibodies was coated onto microplates for antigen capture, while rabbit hyperimmune serum served as detecting antibodies in an indirect assay. The combination of the two monoclonal antibodies did not increase sensitivity synergistically, but in a compensatory fashion, probably because of epitope differences between BEC field strain

The Nature of the Emission Components in the Quasar/NLS1 PG1211+143

We present the study of the emission properties of the quasar PG1211+143, which belongs to the class of Narrow Line Seyfert 1 galaxies. On the basis of observational data analyzed by us and collected from the literature, we study the temporal and spectral variability of the source in the optical/UV/X-ray bands and we propose a model that explains the spectrum emitted in this broad energy range. In this model, the intrinsic emission originating in the warm skin of the accretion disk is responsible for the spectral component that is dominant in the softest X-ray range. The shape of reflected spectrum as well as Fe K line detected in hard X-rays require the reflecting medium to be mildly ionized (xi~500). We identify this reflector with the warm skin of the disk and we show that the heating of the skin is consistent with the classical alpha P_{tot} prescription, while alpha P_{gas} option is at least two orders of magnitude too low to provide the required heating. We find that the mass of the central black hole is relatively small (M_BH~10^7- 10^8 Msun, which is consistent with the Broad Line Region mapping results and characteristic for NLS1 class.Comment: 22 pages, 10 figures, accepted to Ap

Modeling of Atmospheric-Pressure Dielectric Barrier Discharges in Argon with Small Admixtures of Tetramethylsilane

A time-dependent, spatially one-dimensional fluid-Poisson model is applied to analyze the impact of small amounts of tetramethylsilane (TMS) as precursor on the discharge characteristics of an atmospheric-pressure dielectric barrier discharge (DBD) in argon. Based on an established reaction kinetics for argon, it includes a plasma chemistry for TMS, which is validated by measurements of the ignition voltage at the frequency f=86.2kHz for TMS amounts of up to 200 ppm. Details of both a reduced Ar-TMS reaction kinetics scheme and an extended plasma-chemistry model involving about 60 species and 580 reactions related to TMS are given. It is found that good agreement between measured and calculated data can be obtained, when assuming that 25% of the reactions of TMS with excited argon atoms with a rate coefficient of 3.0×10$^{−16}$m$^{3}$/s lead to the production of electrons due to Penning ionization. Modeling results for an applied voltage U$_{a,0}$=4kV show that TMS is depleted during the residence time of the plasma in the DBD, where the percentage consumption of TMS decreases with increasing TMS fraction because only a finite number of excited argon species is available to dissociate and/or ionize the precursor via energy transfer. Main species resulting from that TMS depletion are presented and discussed. In particular, the analysis clearly indicates that trimethylsilyl cations can be considered to be mainly responsible for the film formation

The mass of the black hole in RE J1034+396

The black hole mass measurement in active galaxies is a challenge, particularly in sources where the reverberation method cannot be applied. We aim to determine the black hole mass in a very special object, RE J1034+396, one of the two AGN with QPO oscillations detected in X-rays, and a single bright AGN with optical band totally dominated by starlight. We fit the stellar content using the code starlight, and the broad band disk contribution to optical/UV/X-ray emission is modeled with optxagnf. We also determine the black hole mass using several other independent methods. Various methods give contradictory results. Most measurements of the blacc hole mass are in the range 1.e6-1.e7 Msun, and the measurements based on dynamics give higher values than measurements based on Hbeta and Mg II emission lines.Comment: A&A, in pres

Solutions for 10,000 Eclipsing Binaries in the Bulge Fields of OGLE II Using DEBiL

We have developed a fully-automated pipeline for systematically identifying and analyzing eclipsing binaries within large datasets of light curves. The pipeline is made up of multiple tiers which subject the light curves to increasing levels of scrutiny. After each tier, light curves that did not conform to a given criteria were filtered out of the pipeline, reducing the load on the following, more computationally intensive tiers. As a central component of the pipeline, we created the fully automated Detached Eclipsing Binary Light curve fitter (DEBiL), which rapidly fits large numbers of light curves to a simple model. Using the results of DEBiL, light curves of interest can be flagged for follow-up analysis. As a test case, we analyzed the 218699 light curves within the bulge fields of the OGLE II survey and produced 10862 model fits. We point out a small number of extreme examples as well as unexpected structure found in several of the population distributions. We expect this approach to become increasingly important as light curve datasets continue growing in both size and number.Comment: Accepted for publication in ApJ, 36 pages, 15 figures, 5 tables. See http://cfa-www.harvard.edu/~jdevor/DEBiL.html for high-resolution figures and further informatio

A Composite HST Spectrum of Quasars

We construct a composite quasar spectrum from 284 HST FOS spectra of 101 quasars with redshifts z > 0.33. The spectrum covers the wavelengths between 350 and 3000 A in the rest frame. There is a significant steepening of the continuum slope around 1050 A. The continuum between 1050 and 2200 A can be modeled as a power law with alpha = -0.99. For the full sample the power-law index in the extreme ultraviolet (EUV) between 350 and 1050 A is alpha = -1.96. The continuum flux in the wavelengths near the Lyman limit shows a depression of about 10 percent. The break in the power-law index and the slight depression of the continuum near the Lyman limit are features expected in Comptonized accretion-disk spectra.Comment: 10 figures To appear in the February 1, 1997, issue of the Ap.

Strong Field Gravity and X-Ray Observations of 4U1820-30

The behavior of quasi-periodic oscillations (QPOs) at frequencies near 1 kHz in the x-ray emission from the neutron star x-ray binary 4U1820-30 has been interpreted as evidence for the existence of the marginally stable orbit, a key prediction of strong-field general relativity. The signature of the marginally stable orbit is a saturation in QPO frequency, assumed to track inner disk radius, versus mass accretion rate. Previous studies of 4U1820-30 have used x-ray count rate as an indicator of mass accretion rate. However, x-ray count rate is known to not correlate robustly with mass accretion rate or QPO frequency in other sources. Here, we examine the QPO frequency dependence on two other indicators of mass accretion rate: energy flux and x-ray spectral shape. Using either of these indicators, we find that the QPO frequency saturates at high mass accretion rates. We interpret this as strong evidence for the existence of the marginally stable orbit.Comment: accepted to the Astrophysical Journal Letters, 7 page

A ~4.6 h quasi-periodic oscillation in the BL Lacertae PKS 2155-304?

We report a possible detection of an ~4.6-hour quasi-periodic oscillation (QPO) in the 0.3-10 keV emission of the high-energy peaked blazar PKS 2155-304 from a 64 ks observation by the XMM-Newton EPIC/pn detector. We identify a total modulation of ~5% in the light curve and confirm that nominal period by periodogram, structure function and wavelet analyses. The limited light curve duration allows the capture of only 3.8 cycles of this oscillation and thus precludes a very strong claim for this QPO, despite a nominally high (>3 sigma) statistical significance. We briefly discuss models capable of producing an X-ray QPO of such a period in a blazar.Comment: 4 pages, 6 figures, accepted for publication in A&A Letter

Novel Cauchy-horizon instability

The evolution of weak discontinuity is investigated on horizons in the $n$-dimensional static solutions in the Einstein-Maxwell-scalar-$\Lambda$ system, including the Reissner-Nordstr\"om-(anti) de Sitter black hole. The analysis is essentially local and nonlinear. We find that the Cauchy horizon is unstable, whereas both the black-hole event horizon and the cosmological event horizon are stable. This new instability, the so-called kink instability, of the Cauchy horizon is completely different from the well-known ``infinite-blueshift'' instability. The kink instability makes the analytic continuation beyond the Cauchy horizon unstable.Comment: 6 pages, 1 figure, final version to appear in Physical Review

Discovery of a peculiar Cepheid-like star towards the northern edge of the Small Magellanic Cloud

For seven years, the EROS-2 project obtained a mass of photometric data on variable stars. We present a peculiar Cepheid-like star, in the direction of the Small Magellanic Cloud, which demonstrates unusual photometric behaviour over a short time interval. We report on data of the photometry acquired by the MARLY telescope and spectroscopy from the EFOSC instrument for this star, called EROS2 J005135-714459(sm0060n13842), which resembles the unusual Cepheid HR 7308. The light curve of our target is analysed using the Analysis of Variance method to determine a pulsational period of 5.5675 days. A fit of time-dependent Fourier coefficients is performed and a search for proper motion is conducted. The light curve exhibits a previously unobserved and spectacular change in both mean magnitude and amplitude, which has no clear theoretical explanation. Our analysis of the spectrum implies a radial velocity of 104 km s$^{-1}$ and a metallicity of -0.4$\pm$0.2 dex. In the direction of right ascension, we measure a proper motion of 17.4$\pm$6.0 mas yr$^{-1}$ using EROS astrometry, which is compatible with data from the NOMAD catalogue. The nature of EROS2 J005135-714459(sm0060n13842) remains unclear. For this star, we may have detected a non-zero proper motion for this star, which would imply that it is a foreground object. Its radial velocity, pulsational characteristics, and photometric data, however, suggest that it is instead a Cepheid-like object located in the SMC. In such a case, it would present a challenge to conventional Cepheid models.Comment: Correction of typos in the abstrac