1,076 research outputs found
Neonatal high protein intake enhances neonatal growth without significant adverse renal effects in spontaneous IUGR piglets.
In humans, early high protein (HP) intake has been recommended to prevent postnatal growth restriction and complications of intrauterine growth restriction (IUGR). However, the impact of such a strategy on the kidneys remains unknown, while significant renal hypertrophy, proteinuria, and glomerular sclerosis have been demonstrated in few experimental studies. The objective of this study was to evaluate the effects of a neonatal HP formula on renal structure in IUGR piglets. Spontaneous IUGR piglets were randomly allocated to normal protein (NP, javax.xml.bind.JAXBElement@68d5845e = 10) formula or to HP formula (+50% protein content, javax.xml.bind.JAXBElement@3e768c15 = 10) up to day 28 after birth. Body weight, body composition, renal functions, and structure were assessed at the end of the neonatal period. While birth weights were similar, 28-day-old HP piglets were 18% heavier than NP piglets ( javax.xml.bind.JAXBElement@206b72ec < javax.xml.bind.JAXBElement@7f241a6d 0.01). Carcass protein content was 22% higher in HP than in NP offspring ( javax.xml.bind.JAXBElement@3b9786a3 < javax.xml.bind.JAXBElement@318ba3e0 0.01). Despite a HP intake, kidney weight and glomerular fibrosis were unaltered in HP piglets. Only a 20% increase in glomerular volume was noted in HP piglets ( javax.xml.bind.JAXBElement@4a0c5b2f < 0.05) and restricted to the inner cortical area nephrons ( javax.xml.bind.JAXBElement@1524c771 = javax.xml.bind.JAXBElement@1281f9e8 0.03). Plasma urea/creatinine ratio and proteinuria were unchanged in HP piglets. In conclusion, neonatal HP feeding in IUGR piglets significantly enhanced neonatal growth and tissue protein deposition but mildly affected glomerular volume. It can be speculated that a sustained tissue protein anabolism in response to HP intake have limited single nephron glomerular hyperfiltration
Performance of Glass Resistive Plate Chambers for a high granularity semi-digital calorimeter
A new design of highly granular hadronic calorimeter using Glass Resistive
Plate Chambers (GRPCs) with embedded electronics has been proposed for the
future International Linear Collider (ILC) experiments. It features a 2-bit
threshold semi-digital read-out. Several GRPC prototypes with their electronics
have been successfully built and tested in pion beams. The design of these
detectors is presented along with the test results on efficiency, pad
multiplicity, stability and reproducibility.Comment: 16 pages, 15 figure
Construction and commissioning of a technological prototype of a high-granularity semi-digital hadronic calorimeter
A large prototype of 1.3m3 was designed and built as a demonstrator of the
semi-digital hadronic calorimeter (SDHCAL) concept proposed for the future ILC
experiments. The prototype is a sampling hadronic calorimeter of 48 units. Each
unit is built of an active layer made of 1m2 Glass Resistive Plate
Chamber(GRPC) detector placed inside a cassette whose walls are made of
stainless steel. The cassette contains also the electronics used to read out
the GRPC detector. The lateral granularity of the active layer is provided by
the electronics pick-up pads of 1cm2 each. The cassettes are inserted into a
self-supporting mechanical structure built also of stainless steel plates
which, with the cassettes walls, play the role of the absorber. The prototype
was designed to be very compact and important efforts were made to minimize the
number of services cables to optimize the efficiency of the Particle Flow
Algorithm techniques to be used in the future ILC experiments. The different
components of the SDHCAL prototype were studied individually and strict
criteria were applied for the final selection of these components. Basic
calibration procedures were performed after the prototype assembling. The
prototype is the first of a series of new-generation detectors equipped with a
power-pulsing mode intended to reduce the power consumption of this highly
granular detector. A dedicated acquisition system was developed to deal with
the output of more than 440000 electronics channels in both trigger and
triggerless modes. After its completion in 2011, the prototype was commissioned
using cosmic rays and particles beams at CERN.Comment: 49 pages, 41 figure
NORA moving forward: Developing an oyster restoration network in Europe to support the Berlin Oyster Recommendation
1. The Native Oyster Restoration Alliance (NORA) supports the protection and ecological restoration of the native European oyster, Ostrea edulis, and its habitat across its current and historical biogeographical range. NORA works to overcome barriers to the conservation, restoration, and recovery of the European oyster by providing a platform for the NORA community to collaborate and participate in knowledge exchange. NORA seeks to support responsible restoration practice, in compliance with biosecurity and sustainability.
2. Against this background, the NORA community formulated a series of specific recommendations, the Berlin Oyster Recommendation, to support native oyster restoration by developing and applying best practice with the aim to recover healthy and resilient marine ecosystems. In combination with the Standards for Ecological Restoration (SER) and the Restoration Guidelines for Shellfish Reefs, the Berlin Oyster Recommendation is a relevant tool for successful and sustainable oyster restoration in Europe.
3. The establishment of NORA working groups will support the implementation and further development of the six corresponding recommendations. Current NORA working groups cover site selection, biosecurity, production, and monitoring. The site selection working group will address the identification of suitable sites for oyster restoration to support policy relevant decision making and the conservation, reinforcement, or reintroduction of native oysters. The biosecurity working group will develop biosecurity guidelines for native oyster restoration in Europe. The production working group will assess the potential of standards for seed oyster production and supply in order to enhance production appropriate for restoration purposes. In close collaboration with the Native Oyster Network – UK & Ireland (NON), the monitoring working group will produce a monitoring guidelines handbook to provide metrics and methods that will be suitable across the range of O. edulis projects in Europe for the documentation of restoration success and ecosystem recovery.
4. The Berlin Oyster Recommendation was examined and interpreted by NORA experts in the context of the further development of joint guidelines for the practice of successful and sustainable native oyster restoration
Pion and proton showers in the CALICE scintillator-steel analogue hadron calorimeter
Showers produced by positive hadrons in the highly granular CALICE
scintillator-steel analogue hadron calorimeter were studied. The experimental
data were collected at CERN and FNAL for single particles with initial momenta
from 10 to 80 GeV/c. The calorimeter response and resolution and spatial
characteristics of shower development for proton- and pion-induced showers for
test beam data and simulations using Geant4 version 9.6 are compared.Comment: 26 pages, 16 figures, JINST style, changes in the author list, typos
corrected, new section added, figures regrouped. Accepted for publication in
JINS
Gametogenesis in the Pacific Oyster Crassostrea gigas: A Microarrays-Based Analysis Identifies Sex and Stage Specific Genes
Background: The Pacific oyster Crassostrea gigas (Mollusca, Lophotrochozoa) is an alternative and irregular protandrous hermaphrodite: most individuals mature first as males and then change sex several times. Little is known about genetic and phenotypic basis of sex differentiation in oysters, and little more about the molecular pathways regulating reproduction. We have recently developed and validated a microarray containing 31,918 oligomers (Dheilly et al., 2011) representing the oyster transcriptome. The application of this microarray to the study of mollusk gametogenesis should provide a better understanding of the key factors involved in sex differentiation and the regulation of oyster reproduction. Methodology/Principal Findings: Gene expression was studied in gonads of oysters cultured over a yearly reproductive cycle. Principal component analysis and hierarchical clustering showed a significant divergence in gene expression patterns of males and females coinciding with the start of gonial mitosis. ANOVA analysis of the data revealed 2,482 genes differentially expressed during the course of males and/or females gametogenesis. The expression of 434 genes could be localized in either germ cells or somatic cells of the gonad by comparing the transcriptome of female gonads to the transcriptome of stripped oocytes and somatic tissues. Analysis of the annotated genes revealed conserved molecular mechanisms between mollusks and mammals: genes involved in chromatin condensation, DNA replication and repair, mitosis and meiosis regulation, transcription, translation and apoptosis were expressed in both male and female gonads. Most interestingly, early expressed male-specific genes included bindin and a dpy-30 homolog and female-specific genes included foxL2, nanos homolog 3, a pancreatic lipase related protein, cd63 and vitellogenin. Further functional analyses are now required in order to investigate their role in sex differentiation in oysters. Conclusions/Significance: This study allowed us to identify potential markers of early sex differentiation in the oyster C. gigas, an alternative hermaphrodite mollusk. We also provided new highly valuable information on genes specifically expressed by mature spermatozoids and mature oocytes
Shower development of particles with momenta from 15 GeV to 150 GeV in the CALICE scintillator-tungsten hadronic calorimeter
We present a study of showers initiated by electrons, pions, kaons, and
protons with momenta from 15 GeV to 150 GeV in the highly granular CALICE
scintillator-tungsten analogue hadronic calorimeter. The data were recorded at
the CERN Super Proton Synchrotron in 2011. The analysis includes measurements
of the calorimeter response to each particle type as well as measurements of
the energy resolution and studies of the longitudinal and radial shower
development for selected particles. The results are compared to Geant4
simulations (version 9.6.p02). In the study of the energy resolution we include
previously published data with beam momenta from 1 GeV to 10 GeV recorded at
the CERN Proton Synchrotron in 2010.Comment: 35 pages, 21 figures, 8 table
Searches at HERA for Squarks in R-Parity Violating Supersymmetry
A search for squarks in R-parity violating supersymmetry is performed in e^+p
collisions at HERA at a centre of mass energy of 300 GeV, using H1 data
corresponding to an integrated luminosity of 37 pb^(-1). The direct production
of single squarks of any generation in positron-quark fusion via a Yukawa
coupling lambda' is considered, taking into account R-parity violating and
conserving decays of the squarks. No significant deviation from the Standard
Model expectation is found. The results are interpreted in terms of constraints
within the Minimal Supersymmetric Standard Model (MSSM), the constrained MSSM
and the minimal Supergravity model, and their sensitivity to the model
parameters is studied in detail. For a Yukawa coupling of electromagnetic
strength, squark masses below 260 GeV are excluded at 95% confidence level in a
large part of the parameter space. For a 100 times smaller coupling strength
masses up to 182 GeV are excluded.Comment: 32 pages, 14 figures, 3 table
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