Cryopreservation time does not decrease follicular viability in ovarian tissue frozen for fertility preservation

OBJECTIVE: To determine the effect of storage duration on cryopreserved ovarian tissue using fresh and frozenthawed samples. METHODS: Seventeen fertile patients underwent an ovarian biopsy during elective laparoscopic tubal ligation. The tissue sample was divided into three parts: one part was processed fresh (FG), and two were slowly frozen, cryopreserved for 30 (G30) or 180 days (G180), thawed and analyzed. Follicular density, follicular viability, and steroidogenic capacity were assessed. RESULTS: We observed no differences between the groups in follicular density, which was assessed in hematoxylin and eosin-stained tissue sections. A heterogeneous follicular distribution was observed in the parenchyma, with a mean density of 361.3±255.4, 454.9±676.3, and 296.8±269.0 follicles/mm3 for FG, G30 and G180, respectively (p = 0.46). Follicular viability was greater in FG (93.4%) when compared with the cryopreserved tissues (70.8% for G30 (p<0.001) and 78.4% for G180 (p<0.001)), with no difference in viability between the frozen samples (p>0.05). The steroidogenic capacity of the tissue was not significantly reduced following cryopreservation. CONCLUSION: The slow freezing procedures used for ovarian cryopreservation are capable of preserving follicular viability and maintaining the steroidogenic capacity of the tissue despite a roughly 30% decrease in follicular viability. Furthermore, short-term storage of ovarian tissue does not appear to compromise follicle integrity

IoT Smart Collect - Routing Process and Driver Guidance

Waste collection is a traditional process that involves a driver collecting waste from a set of designated deposits based on a pre-determined route. The authors present a new approach that utilizes Artificial Intelligence to define the route based on the occupancy volume of the deposits. The new process involves the use of a mobile application to assist the driver during the journey. The application communicates with the central system to receive information on the next route, calculates the best possible route considering traffic laws and road conditions, and guides the driver throughout the journey. The application also provides real-time updates on the driver’s progress and allows the driver to provide feedback All collected data is stored and can be consulted and explored through lists, graphs, and filtering options. The authors believe that the new approach will improve the efficiency of waste collection and provide a better experience for the driver.info:eu-repo/semantics/publishedVersio

A Etnobotânica como uma ferramenta no ensino e aprendizagem de Química: uma proposta de ensino contextualizado - Ação PIBID/REUNE/UFPE

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Multitask ATPases (NBDs) of bacterial ABC importers type I and their interspecies exchangeability

We would like to thank Herminia de Lencastre (ITQB-AX NOVA), Adriano Henriques (ITQB-AX NOVA) and PaulaTamagnini (i3S) for the gift of chromosomal DNA, Paulo Tavares (I2BC-CNRS) and Adriano Henriques for the gift of flagellin and anti-sigmaA antibodies, respectively, and Diogo Pereira for carrying out some growth kinetic assays. This work was supported by the FundacAo para a Ciencia e a Tecnologia [Grant Numbers PTDC/BIA-MIC/30696/2017 to ISN and UID/Multi/04378/2019 to Unidade de Ciencias Biomoleculares Aplicadas UCIBIO; and fellowship PD/BD/105737/2014 to F.L.].ATP-binding cassette (ABC) type I importers are widespread in bacteria and play a crucial role in its survival and pathogenesis. They share the same modular architecture comprising two intracellular nucleotide-binding domains (NBDs), two transmembrane domains (TMDs) and a substrate-binding protein. The NBDs bind and hydrolyze ATP, thereby generating conformational changes that are coupled to the TMDs and lead to substrate translocation. A group of multitask NBDs that are able to serve as the cellular motor for multiple sugar importers was recently discovered. To understand why some ABC importers share energy-coupling components, we used the MsmX ATPase from Bacillus subtilis as a model for biological and structural studies. Here we report the first examples of functional hybrid interspecies ABC type I importers in which the NBDs could be exchanged. Furthermore, the first crystal structure of an assigned multitask NBD provides a framework to understand the molecular basis of the broader specificity of interaction with the TMDs.publishersversionpublishe

Accuracy of computer-aided image analysis in the diagnosis of odontogenic cysts:a systematic review

This study aimed to search for scientific evidence concerning the accuracy of computer-assisted analysis for diagnosing odontogenic cysts. A systematic review was conducted according to the PRISMA statements and considering eleven databases, including the grey literature. Protocol was registered in PROSPERO (CRD 42020189349). The PECO strategy was used to define the eligibility criteria and only studies involving diagnostic accuracy were included. Their risk of bias was investigated using the Joanna Briggs Institute Critical Appraisal tool. Out of 437 identified citations, five papers, published between 2006 and 2019, fulfilled the criteria and were included in this systematic review. A total of 5,264 images from 508 lesions, classified as radicular cyst, odontogenic keratocyst, lateral periodontal cyst, glandular odontogenic cyst, or dentigerous cyst, were analyzed. All selected articles scored low risk of bias. In three studies, the best performances were achieved when the two subtypes of odontogenic keratocysts (solitary or syndromic) were pooled together, the case-wise analysis showing a success rate of 100% for odontogenic keratocysts and radicular cysts, in one of them. In two studies, the dentigerous cyst was associated with the majority of misclassifications, and its omission from the dataset improved significantly the classification rates. The overall evaluation showed all studies presented high accuracy rates of computer-aided systems in classifying odontogenic cysts in digital images of histological tissue sections. However, due to the heterogeneity of the studies, a meta-analysis evaluating the outcomes of interest was not performed and a pragmatic recommendation about their use is not possible

Questionnaires measuring movement behaviours in adults and older adults: content description and measurement properties. A systematic review

Background Sleep, sedentary behaviour and physical activity are constituent parts of a 24h period and there are several questionnaires to measure these movement behaviours, the objective was to systematically review the literature on content and measurement properties of self- and proxy-reported questionnaires measuring movement behaviours in adults and older adults. Methods The databases PubMed, CINAHL, PsycINFO and SPORTDiscus were systematically searched until April 2021. Articles were included if: the questionnaires were design for adults and older adults; the sample size for validity studies had at least 50 participants; at least, both validity and test-retest reliability results of questionnaire that were developed specifically to measure the amount of sleep, sedentary behaviour or physical activity, or their combination were reported; and articles had to be written in English, Spanish, French, Portuguese, German, Italian or Chinese. Findings and conclusions Data extraction, results, studies’ quality, and risk of bias were evaluated using the Consensus-based Standards for the selection of health Measurement INstruments (COSMIN) guidelines. Fifty-five articles were included in this review, describing 60 questionnaires. None of the questionnaires showed adequate criterion validity and adequate reliability, simultaneously; 68.3% showed adequate content validity. The risk of bias for criterion validity and reliability were very low in 72.2% and 23.6% of the studies, respectively. Existing questionnaires have insufficient measurement properties and frequent methodologic limitations, and none was developed considering the 24h movement behaviour paradigm. The lack of valid and reliable questionnaires assessing 24h movement behaviours in an integrated way, precludes accurate monitoring and surveillance systems of 24h movement behaviours.info:eu-repo/semantics/publishedVersio

Redução na força muscular e capacidade funcional em pacientes fisicamente inativos com lúpus eritematoso sistêmico de início juvenil, apesar de doença muito leve

ResumoObjetivoComparar a força muscular (ou seja, a força muscular dos membros superiores e inferiores) e a capacidade funcional de pacientes fisicamente inativos com lúpus eritematoso sistêmico de início juvenil (LESJ) com controles saudáveis (CTRL).MétodosEstudo transversal cuja amostra foi composta por 19 pacientes com LESJ (entre 9 e 18 anos) e 15 CTRL pareados por idade, sexo, índice de massa corporal (IMC) e nível de atividade física (avaliada através do uso de acelerômetros). A força dos membros superiores e inferiores foi avaliada pelo teste de uma repetição máxima (1‐RM). A força isométrica foi avaliada através do uso de um dinamômetro. A capacidade funcional foi avaliada pelo Timed‐stands test (TST) e Timed‐up‐and‐go test (TUG).ResultadosQuando comparados com os CTRL, os pacientes com LESJ apresentaram menor força em 1‐RM no Leg press e supino (p=0,026 e p=0,008, respectivamente) e uma tendência a menor força de preensão manual (p=0,052). Os pacientes com LESJ apresentaram menores escores no TST (p=0,036) e uma tendência a maior pontuação no TUG (p=0,070), quando comparados com o grupo CTRL.ConclusãoPacientes com LESJ, fisicamente inativos, com doença muito leve mostraram redução na força muscular e capacidade funcional quando comparados com controles saudáveis pareados por níveis de atividade física. Esses achados sugerem que pacientes com LESJ podem apresentar mais efeitos deletérios por manter um estilo de vida fisicamente inativo do que controles saudáveis. Além disso, alguns efeitos “residuais” subclínicos da doença ou do tratamento farmacológico parecem afetar pacientes com LESJ, mesmo com uma doença bem controlada.AbstractObjectiveTo compare muscle strength (i.e. lower‐ and upper‐body strength) and function between physically inactive childhood‐onset systemic lupus erythematosus patients (C‐SLE) and healthy controls (CTRL).MethodsThis was a cross‐sectional study and the sample consisted of 19 C‐SLE (age between 9 to 18 years) and 15 CTRL matched by age, sex, body mass index (BMI), and physical activity levels (assessed by accelerometry). Lower‐ and upper‐body strength was assess by the one‐repetition‐maximum (1‐RM) test. Isometric strength was assessed through a handgrip dynamometer. Muscle function was evaluated by the timed‐stands test (TST) and the timed‐up‐and‐go test (TUG).ResultsWhen compared with CTRL, C‐SLE showed lower leg‐press and bench‐press 1‐RM (p=0.026 and p=0.008, respectively), and a tendency towards lower handgrip strength (p=0.052). C‐SLE showed lower TST scores (p=0.036) and a tendency towards higher TUG scores (p=0.070) when compared with CTRL.ConclusionPhysically inactive C‐SLE patients with very mild disease showed reduced muscle strength and functionality when compared with healthy controls matched by physical activity levels. These findings suggest C‐SLE patients may greatly suffer from a physically inactive lifestyle than healthy controls do. Moreover, some sub‐clinical “residual” effect of the disease or its pharmacological treatment seems to affect C‐SLE patients even with a well‐controlled disease

Visão geral sobre preservação da fertilidade feminina depois do câncer

ResumoA ação citotóxica de tratamentos antineoplásicos muito frequentemente implica sérios danos às gônadas e consequências secundárias ao hipoestrogenismo, como osteoporose, infertilidade e falência ovariana prematura, são esperadas. A oncofertilidade aparece como uma área multidisciplinar que se dedica ao desenvolvimento de estratégias para a redução de sequelas terapêuticas em sobreviventes de câncer, em última análise, com vistas à manutenção de sua qualidade de vida e à possibilidade da procriação biológica. Este artigo tem como objetivo apresentar uma visão geral sobre as opções possíveis para a preservação da fertilidade feminina em pacientes com câncer e as perspectivas em oncofertilidade.AbstractThe cytotoxic action of antineoplastic treatments very frequently implies serious damage to the gonads, and consequences due to the hypoestrogenism, such as osteoporosis, infertility and premature ovarian failure, are expected. Oncofertility appears as a new multidisciplinary area, which is dedicated to the development of strategies for the reduction of therapeutic sequels in cancer survivals, ultimately aiming the maintenance of their quality of life and the possibility of biological procreation. This article aims to present an overview of possible options for female fertility preservation after cancer and future perspectives in oncofertility

FORMAÇÃO INICIAL DOCENTE E ESTÁGIO CURRICULAR SUPERVISIONADO NA LICENCIATURA EM QUÍMICA: PERCEPÇÕES E EXPERIÊNCIAS DE ALUNOS CONCLUINTES

This study aims to analyze the perception of students about the Supervised Curricular Internship in the Teacher formation course in Chemistry, based on their experiences, and discuss the internship as a theoretical-practical space and for reflection on teaching. Based on the reflection on the internship the criticism of the reductionist understanding of this as the time of practice, a qualitative-descriptive study was developed, having as a locus the teacher formation course in Chemistry of a federal institution of higher education in the interior of the state of Ceará, Brazil, with the participation of three undergraduate students who answered five subjective questions about the internship, the answers being analyzed from their meanings and senses. The results indicate in the investigated context the predominance of internships as bureaucratic practice, imitation of models distant from the reflexive-investigative perspective, as well as the critical view of the undergraduate students about the limits of this formative perspective. However, even in the face of the adversities experienced during the internship, students perceive it as an important formative moment for their initial formation.El estudio tiene como objetivo analizar la percepción de los estudiantes de pregrado sobre la Pasantía Curricular Supervisada en la Licenciatura de Química a partir de sus experiencias, y discutir la pasantía como espacio teórico-práctico y de reflexión sobre la docencia. A partir de la reflexión sobre la pasantía y la crítica a la comprensión reduccionista de esta como el tiempo de la práctica, se desarrolló un estudio cualitativo-descriptivo, teniendo como locus la Licenciatura en Química de una institución federal de educación superior en el interior de Ceará, con la participación de tres estudiantes de pregrado que respondieron a cinco preguntas subjetivas sobre la pasantía, analizándose las respuestas desde sus significados y sentidos. Los resultados indican en el contexto investigado el predominio de las pasantías como práctica burocrática, imitación de modelos y distante de la perspectiva reflexiva-investigadora, así como la mirada crítica de los estudiantes sobre los límites de esta perspectiva formativa. Sin embargo, incluso ante las adversidades vividas durante la pasantía, los estudiantes lo perciben como un momento importante para su formación inicial.O estudo objetiva analisar a percepção de licenciandos sobre o Estágio Curricular Supervisionado na Licenciatura em Química a partir de suas experiências, e discutir o estágio como um espaço teórico-prático e de reflexão sobre a docência. Partindo da reflexão sobre o estágio e a crítica à compreensão reducionista deste como a hora da prática, foi desenvolvido um estudo qualitativo-descritivo, tendo como lócus o curso de Licenciatura em Química de uma instituição federal de ensino superior no interior do Ceará, com a participação de três licenciandos que responderam a cinco perguntas subjetivas sobre o estágio, sendo as respostas analisadas a partir de suas significações e sentidos. Os resultados indicam no contexto investigado a predominância de estágios como prática burocrática, imitação de modelos e distante da perspectiva reflexivo-investigativa, assim como a visão crítica dos licenciandos acerca dos limites dessa perspectiva formativa. Contudo, mesmo diante das adversidades vivenciadas ao longo do estágio, os alunos o percebem como um momento importante para sua formação inicial.