Identificação de neomacho de Jundiá Rhamdia quelen (Quoy e Gaimard, 1824) através do teste de progênie

TCC (graduação) - Universidade Federal de Santa Catarina. Centro de Ciências Agrárias. Curso de Engenharia de Aquicultura.O objetivo deste estudo foi identificar neomachos de Rhamdia quelen através de teste de progênie. Esse é um método indireto para a produção de lotes monosexo, que evita a aplicação de hormônio nos peixes destinados ao consumo humano, uma vez que o esperado no cruzamento entre neomachos e fêmeas normais são proles 100% femininas. Para os testes de progênie, machos fenotípicos cultivados por oito meses foram selecionados de um lote submetido à masculinização por ingestão de 17α-metil-testosterona (MT) incorporado na ração na dose de 60mg/kg. Cinco machos foram cruzados com um “pool” de ovos de três fêmeas normais e as proles resultantes de cada cruzamento foram cultivadas por 150 dias para que a sexagem das gônadas pudesse ser realizada através da técnica do imprint. O teste do qui-quadrado evidenciou que um macho originou prole com maior proporção sexual de fêmeas (P<0,05), sendo 75,56%. Apesar de não serem proles 100% femininas, existe um forte indício de que este macho seja neomacho, porque a proporção sexual das demais proles apresentou maior presença de machos ou foi balanceada (1:1)

The Legal Order And İts Relation With The Power And The Economy İn The Current Brazilian Scenery, İnstrumentality a priori And Rationality a posteriori

The Brazilian Legal system and its relation with the power in the current Brazilian scenery, and also the use of a posteriori rationality of the judge and the priori instrumentality of the legislator and of the Executive, is the theme of the present study. Its goal is to show a brief historical evolution of the Law and the State, the Power aspects, the forms of State and its acting with the origin of population. The next step is to present the use of Brazilian Legal System as a form of organization, that is, a priori instrumentality and the evolution from Liberal State to Social State, with significant changes in the legal aspects and in its form of acting. Furthermore, we carry on with the world crisis, especially the one of 1929, whose changes imposed by the end of Second World War (the decline of legal positivism) and also the advances which came with the globalization in 1990. Still considering crisis, it is discussed about the 2008 crisis and its consequences, the European crisis with its high costs of a Social State. A parallelism was done between the economy and the Law and besides that, it was drawn a legal scenery after these evolutions. Regarding these relations of power and the crisis of the power tripartism, we also showed that an ascension of Legal Law can be named as the legal protagonist or, for some, legal activism. These role has some reflexes in the Judicial Discretion, in the active management of processes, in the legal inflation of the Legislative and Executive (provisional measures), in the use of process as a strategy of power, in the legalization of politics and in the creation of binding overviews. Some case studies and considerations about justice were showed. This research is justified because the power, the contemporary legal scenery, the judicial role, the economy and the Law, and the forms of acting of the State (Liberal and Social) are current themes in the study of operators and Law scholars. Thus as these inquiries show great influence in the democratic state of Law

Los pacientes del hospital como recurso pedagógico irremplazable: "Todos los pacientes son neurológicos"

Con el advenimiento de la nueva tecnología es necesario prevenir una eventual distorsión en el proceso de enseñanza-aprendizaje en medicina, especialmente por erróneas suposiciones de carencia de pacientes para la realización de actividades prácticas. Situación que también ha sido detectada desde la actividad docente en nuestra Cátedra

FGF-2 and FGFR-1 immunoexpression in oral tongue squamous cell carcinoma

Orientador: Jacks Jorge JuniorDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de PiracicabaResumo: O fator de crescimento fibroblástico 2 (FGF-2) e o receptor do fator de crescimento fibroblástico 1 (FGFR-1) estão associados à maior capacidade de invasão tumoral, proliferação celular, angiogênese e ao potencial de metástase. Este estudo teve como objetivo investigar a expressão de FGF-2 e FGFR-1 na displasia epitelial oral (DEO) e carcinoma espinocelular de língua (CECL). Foram selecionados retrospectivamente cento e sessenta e sete casos, incluindo 85 espécimes cirúrgicos de pacientes com CECL, provenientes do Hospital Onofre Lopes, Natal, Brasil, além de 46 biópsias incisionais de CECL e 36 de DEO provenientes do arquivo do Laboratório de Patologia Oral da Faculdade de Odontologia de Piracicaba (UNICAMP). Cortes de tecido parafinado foram submetidas à reação imunoistoquímica para FGF-2 e FGFR-1. As lâminas foram escaneadas e a marcação imunoistoquímica foi quantificada digitalmente pelo software Aperio Positive Pixel Count v9. Cinco áreas iguais foram selecionadas no estroma e no epitélio tumoral. Os resultados foram exportados e o score final de cada lesão foi obtido através da soma da porcentagem de pixels fracos, moderados e fortes, gerando um valor que variou de 100 a 300. Os casos foram divididos como tendo "baixa expressão" ou "alta expressão" das proteínas de acordo com a mediana dos grupos. FGF-2 e FGFR-1 foram mais expressos em DEO de alto grau do que em DEO de baixo grau, tanto no epitélio, como nas células do estroma (p<0.05). A sobrevivência doença específica (SDE) em 5 anos foi de 47,3% dos pacientes com CECL. A alta expressão de FGF-2 nas células inflamatórias e mesenquimatosas do estroma foi associada à invasão vascular e pior prognóstico. Pacientes com alta expressão de FGF-2 no estroma apresentaram taxa de SDE em 5 anos de 36,7%, contra 59,3% dos pacientes com baixa expressão (HR: 2,272; IC95%: 1.213-4.254; p=0,008). A alta expressão de FGFR-1 no estroma foi correlacionada com metástases linfonodais e metástases à distância. Pacientes com alta expressão de FGFR-1 no epitélio apresentaram taxa de SDE em 5 anos de 22,9%, contra 75,6% dos pacientes com baixa expressão (HR: 2,594; IC95%: 1,390-4,841; p=0,003). O mesmo aconteceu com FGFR-1 no estroma, com taxa de SDE em 5 anos de 32,9%, contra 64,0% (HR: 3,378; IC95%: 1,816-6,286; p=0,001). A análise multivariada de Cox confirmou que a expressão de FGF-2 no estroma (HR: 2,197; IC95%: 1,128-4,282; p=0,02), FGFR-1 no epitélio (HR: 3,178; IC95%: 1,505-6,709; p=0,002) e FGFR-1 no estroma (HR: 3,041; IC95%: 1,454-6,356; p=0,003) estão fortemente associadas a um maior risco de morte relacionada ao CECL. Em conjunto, nossos achados demonstram que FGF-2 e FGFR-1 desempenham um papel importante na DEO e no CECL, estando associados à presença de metástase e à sobrevivência dos pacientesAbstract: Fibroblast growth factor 2 (FGF-2) and fibroblast growth factor receptor 1 (FGFR-1) expression is associated with tumour invasiveness, cell proliferation, angiogenesis and metastasis potential. This study aimed to investigate FGF-2 and FGFR-1 expression in oral epithelial dysplasia (OED) and tongue squamous cell carcinoma (TSCC). One hundred and sixty-seven cases were retrospectively selected, including 85 surgical specimens of patients with TSCC, from Onofre Lopes Hospital, Natal, Brazil, besides 46 TSCC and 36 OED incisional biopsies from the Laboratory of Oral Pathology, Piracicaba Dental School, University of Campinas (UNICAMP). Tissue sections were submitted to immunohistochemical reaction for FGF-2 and FGFR-1. Slides were scanned and the immunostaining was digitally quantified by the Aperio Positive Pixel Count v9 software. Five areas were selected from the stroma and the epithelium. Results were exported and the final score of each lesion was obtained by the sum of the percentage of weak, moderate and strong pixels, resulting in a value ranging from 100 to 300. Cases were classified as "weak expression" or "high expression" of the proteins, accordingly to the group median. FGF-2 and FGFR-1 were more expressed in high-grade OED than in low-grade OED, either in the stroma or in the epithelium (p<0.05). The 5-year disease-specific survival (DSS) rate was 47.3% of the patients with TSCC. FGF-2 high expression in the inflammatory and mesenchymal cells of the stroma was associated with vascular invasion and worse prognosis. Patients with high expression of FGF-2 in the stroma had a 5-year DSS of 36.7%, against 59.3% of patients with low expression (HR: 2.272; CI(95%): 1.213-4.254; p=0.008). FGFR-1 high expression in the stroma was correlated with lymph node metastasis and distant metastasis. Patients with high expression of FGFR-1 in the tumour had a 5-year DSS of 22.9%, against 75.6% of patients with low expression (HR: 2.594; CI(95%): 1.390-4.841; p=0.003). The same was observed with FGFR-1 in the stroma, with a 5-year DSS of 32.9%, against 64.0% (HR: 3.378; CI(95%): 1.816-6.286; p=0.001). The Cox multivariate analysis confirmed that the expression of FGF-2 in the stroma (HR: 2.197; CI(95%): 1.128-4.282; p=0.02), FGFR-1 in the tumour (HR: 3.178; CI(95%): 1.505-6.709; p=0.002) and FGFR-1 in the stroma (HR: 3.041; CI(95%): 1.454-6.356; p=0.003) are strongly associated with a higher risk of death related to TSCC. Taken together, our findings demonstrate that FGF-2 and FGFR-1 play an important role in OED and TSCC, and are associated with the presence of metastasis and patients disease-specific survivalMestradoEstomatopatologiaMestre em Estomatopatologia33003033009P4CAPE

Automatic Analysis of Facebook Posts and Comments Written in Brazilian Portuguese

Social networks and media are becoming increasingly important sources for knowing people\u27s opinions and sentiments on a wide variety of topics. The huge number of messages published daily in these media makes it impractical to analyze them without the help of natural language processing systems.This article presents an approach to cluster texts by similarity and identifying the sentiments expressed by comments on then (positive, negative and neutral, among others) in an integrated manner. Unlike most of the available studies that focus on the English language and use Twitter as a data source, we treat Brazilian Portuguese posts and comments published on Facebook. The proposed approach employs an unsupervised learning algorithm to group posts and a supervised algorithm to identify the sentiments expressed in comments to posts. In an experimental evaluation, a system that implements the proposed approach showed similar accuracy to that of human evaluators in the tasks of clustering and sentiment analysis, but performed the tasks in much less time