Molecular phylogeny and timing of diversification in Alpine Rhithrogena (Ephemeroptera: Heptageniidae).

BACKGROUND: Larvae of the Holarctic mayfly genus Rhithrogena Eaton, 1881 (Ephemeroptera, Heptageniidae) are a diverse and abundant member of stream and river communities and are routinely used as bio-indicators of water quality. Rhithrogena is well diversified in the European Alps, with a number of locally endemic species, and several cryptic species have been recently detected. While several informal species groups are morphologically well defined, a lack of reliable characters for species identification considerably hampers their study. Their relationships, origin, timing of speciation and mechanisms promoting their diversification in the Alps are unknown. RESULTS: Here we present a species-level phylogeny of Rhithrogena in Europe using two mitochondrial and three nuclear gene regions. To improve sampling in a genus with many cryptic species, individuals were selected for analysis according to a recent DNA-based taxonomy rather than traditional nomenclature. A coalescent-based species tree and a reconstruction based on a supermatrix approach supported five of the species groups as monophyletic. A molecular clock, mapped on the most resolved phylogeny and calibrated using published mitochondrial evolution rates for insects, suggested an origin of Alpine Rhithrogena in the Oligocene/Miocene boundary. A diversification analysis that included simulation of missing species indicated a constant speciation rate over time, rather than any pronounced periods of rapid speciation. Ancestral state reconstructions provided evidence for downstream diversification in at least two species groups. CONCLUSIONS: Our species-level analyses of five gene regions provide clearer definitions of species groups within European Rhithrogena. A constant speciation rate over time suggests that the paleoclimatic fluctuations, including the Pleistocene glaciations, did not significantly influence the tempo of diversification of Alpine species. A downstream diversification trend in the hybrida and alpestris species groups supports a previously proposed headwater origin hypothesis for aquatic insects

Structure of Dark Triad Dirty Dozen Across Eight World Regions

The Dark Triad (i.e., narcissism, psychopathy, Machiavellianism) has garnered intense attention over the past 15 years. We examined the structure of these traits’ measure—the Dark Triad Dirty Dozen (DTDD)—in a sample of 11,488 participants from three W.E.I.R.D. (i.e., North America, Oceania, Western Europe) and five non-W.E.I.R.D. (i.e., Asia, Middle East, non-Western Europe, South America, sub-Saharan Africa) world regions. The results confirmed the measurement invariance of the DTDD across participants’ sex in all world regions, with men scoring higher than women on all traits (except for psychopathy in Asia, where the difference was not significant). We found evidence for metric (and partial scalar) measurement invariance within and between W.E.I.R.D. and non-W.E.I.R.D. world regions. The results generally support the structure of the DTDD

Detection of Social Isolation Based on Meal-Taking Activity and Mobility of Elderly People Living Alone

International audienceObjectives BackgroundSocial isolation is probably one of the most affected health outcomes in the elderly people, particularly those living alone, due to the COVID-19 pandemic. Therefore, we try to identify it by detecting changes in the elderly such as malnutrition and lack of mobility.Material and methodsThe system consists of two types of sensors installed at various locations in the user's home: Passive infrared (PIR) sensors and reed switch sensors. It was implemented for 15 days in the home of a 26-year-old student living alone, as a first step to later be deployed in the home of elderly people.ResultsOur study showed strong similarities between the activities detected by the algorithm and the real activity pattern of the interviewed individual. In addition, the system was able to identify two daily patterns (weekday and weekend) of the person as he is a student and is present in class during the week.ConclusionA system composed of low-cost, unobtrusive, non-intrusive and miniaturized sensors is able to detect meal-taking activity and mobility. These results are an intermediate step in assessing the potential risk of social isolation in older people living alone based on these ADLs

IL-34 and M-CSF form a novel heteromeric cytokine and regulate the M-CSF receptor activation and localization

Interleukin-34 (IL-34) is a newly-discovered homodimeric cytokine that regulates, like Macrophage Colony-Stimulating Factor (M-CSF), the differentiation of the myeloid lineage through M-CSF receptor (M-CSFR) signaling pathways. To date, both cytokines have been considered as competitive cytokines with regard to the M-CSFR. The aim of the present work was to study the functional relationships of these cytokines on cells expressing the M-CSFR. We demonstrate that simultaneous addition of M-CSF and IL-34 led to a specific activation pattern on the M-CSFR, with higher phosphorylation of the tyrosine residues at low concentrations. Similarly, both cytokines showed an additive effect on cellular proliferation or viability. In addition, BIAcore experiments demonstrated that M-CSF binds to IL-34, and molecular docking studies predicted the formation of a heteromeric M-CSF/IL-34 cytokine. A proximity ligation assay confirmed this interaction between the cytokines. Finally, co-expression of the M-CSFR and its ligands differentially regulated M-CSFR trafficking into the cell. This study establishes a new foundation for the understanding of the functional relationship between IL-34 and M-CSF, and gives a new vision for the development of therapeutic approaches targeting the IL-34/M-CSF/M-CSFR axis

Are Fluorescent Organic Nanoparticles Relevant Tools for Tracking Cancer Cells or Macrophages?

Strongly solvatochromic fluorophores are devised, containing alkyl chains and enable to self-assemble as very bright fluorescent organic nanoparticles (FONs) in water (Φf = 0.28). The alkyl chains impart each fluorophore with strongly hydrophobic surroundings, causing distinct emission colors between FONs where the fluorophores are associated, and their disassembled state. Such color change is harnessed to assess the long-term fate of FONs in both cancer cells and monocytes/macrophages. Disintegration of the orange-emitting FONs by monocytes/macrophages is evidenced through the formation of micrometer green-yellowish emitting vesicles. By contrast, cancer cells retain longer the integrity of organic nanoparticles. In both cases, no significant toxicity is detected, making FONs as valuable bioimaging agents for cell tracking with weak risks of deleterious accumulation and low degradation rate

Talking about terrorism: A study of vernacular discourse

This article examines a neglected dimension of the dominant discourse of terrorism, namely, the vernacular, ‘everyday narratives’ of lay members of the public. While there are many studies of elite- and public-level discourses of terrorism, large numbers of public opinion and attitude studies, and a growing number of focus group studies on vernacular understandings of security and citizenship, there are few studies which specifically focus on lay discourses of terrorism. Importantly, there are even fewer studies which employ discursive psychology and conversation analysis as their primary methodological approach. Consequently, we do not know much about how ordinary people speak about in daily conversation – and therefore, how they understand or ‘know’ – what terrorism is, how it manifests, what its causes are, and how it is best dealt with. This article reports on some of the key findings of an empirical study of vernacular discourse about terrorism carried out in Aberystwyth, Wales. It reflects on what these findings tell us about how important political discourses are expressed in different arenas by different actors, how they are consumed and circulate, how they are resisted, how hegemonic they are in practice, and how they reflect cultural dispositions and the politics of legitimacy