Fabrication of living soft matter by symbiotic growth of unicellular microorganisms

We report the fabrication of living soft matter made as a result of the symbiotic relationship of two unicellular microorganisms. The material is composed of bacterial cellulose produced in situ by acetobacter (Acetobacter aceti NCIMB 8132) in the presence of photosynthetic microalgae (Chlamydomonas reinhardtii cc-124), which integrates into a symbiotic consortium and gets embedded in the produced cellulose composite. The same concept of growing living materials can be applied to other symbiotic microorganism pairs similar to the combination of algae and fungi in lichens, which is widespread in Nature. We demonstrate the in situ growth and immobilisation of the C. reinhardtii cells in the bacterial cellulose matrix produced by the simultaneous growth of acetobacter. The effect of the growth media composition on the produced living materials was investigated. The microstructure and the morphology of the produced living biomaterials were dependent on the shape of the growth culture container and media stirring conditions, which control the access to oxygen. As the photosynthetic C. reinhardtii cells remain viable and produce oxygen as they spontaneously integrate into the matrix of the bacterial cellulose generated by the acetobacter, such living materials have the potential for various applications in bio-hydrogen generation from the immobilised microalgae. The proposed approach for building living soft matter can provide new ways of immobilising other commercially important microorganisms in a bacterial cellulose matrix as a result of symbiosis with acetobacter without the use of synthetic binding agents and in turn increase their production efficiency

The Milky Way's bright satellites as an apparent failure of LCDM

We use the Aquarius simulations to show that the most massive subhalos in galaxy-mass dark matter halos in LCDM are grossly inconsistent with the dynamics of the brightest Milky Way dwarf spheroidal galaxies. While the best-fitting hosts of the dwarf spheroidals all have 12 < Vmax < 25 km/s, LCDM simulations predict at least ten subhalos with Vmax > 25 km/s. These subhalos are also among the most massive at earlier times, and significantly exceed the UV suppression mass back to z ~ 10. No LCDM-based model of the satellite population of the Milky Way explains this result. The problem lies in the satellites' densities: it is straightforward to match the observed Milky Way luminosity function, but doing so requires the dwarf spheroidals to have dark matter halos that are a factor of ~5 more massive than is observed. Independent of the difficulty in explaining the absence of these dense, massive subhalos, there is a basic tension between the derived properties of the bright Milky Way dwarf spheroidals and LCDM expectations. The inferred infall masses of these galaxies are all approximately equal and are much lower than standard LCDM predictions for systems with their luminosities. Consequently, their implied star formation efficiencies span over two orders of magnitude, from 0.2% to 20% of baryons converted into stars, in stark contrast with expectations gleaned from more massive galaxies. We explore possible solutions to these problems within the context of LCDM and find them to be unconvincing. In particular, we use controlled simulations to demonstrate that the small stellar masses of the bright dwarf spheroidals make supernova feedback an unlikely explanation for their low inferred densities.Comment: 18 pages, 10 figures; matches version published in MNRA

Methodology for High-Throughput Field Phenotyping of Canopy Temperature Using Airborne Thermography

Lower canopy temperature (CT), resulting from increased stomatal conductance, has been associated with increased yield in wheat. Historically, CT has been measured with hand-held infrared thermometers. Using the hand-held CT method on large field trials is problematic, mostly because measurements are confounded by temporal weather changes during the time required to measure all plots. The hand-held CT method is laborious and yet the resulting heritability low, thereby reducing confidence in selection in large scale breeding endeavors. We have developed a reliable and scalable crop phenotyping method for assessing CT in large field experiments. The method involves airborne thermography from a manned helicopter using a radiometrically-calibrated thermal camera. Thermal image data is acquired from large experiments in the order of seconds, thereby enabling simultaneous measurement of CT on potentially 1000s of plots. Effects of temporal weather variation when phenotyping large experiments using hand-held infrared thermometers are therefore reduced. The method is designed for cost-effective and large-scale use by the non-technical user and includes custom-developed software for data processing to obtain CT data on a single-plot basis for analysis. Broad-sense heritability was routinely >0.50, and as high as 0.79, for airborne thermography CT measured near anthesis on a wheat experiment comprising 768 plots of size 2 × 6 m. Image analysis based on the frequency distribution of temperature pixels to remove the possible influence of background soil did not improve broad-sense heritability. Total image acquisition and processing time was ca. 25 min and required only one person (excluding the helicopter pilot). The results indicate the potential to phenotype CT on large populations in genetics studies or for selection within a plant breeding program.This research was funded by the Australian Government National Collaborative Research Infrastructure Strategy (Australian Plant Phenomics Facility) and the Grains Research and Development Corporation (GRDC)

Small-Scale Structure in the SDSS and LCDM: Isolated L* Galaxies with Bright Satellites

We use a volume-limited spectroscopic sample of isolated galaxies in the Sloan Digital Sky Survey (SDSS) to investigate the frequency and radial distribution of luminous (M_r <~ -18.3) satellites like the Large Magellanic Cloud (LMC) around ~L* Milky Way analogs and compare our results object-by-object to LCDM predictions based on abundance matching in simulations. We show that 12% of Milky Way-like galaxies host an LMC-like satellite within 75 kpc (projected), and 42 % within 250 kpc (projected). This implies ~10% have a satellite within the distance of the LMC, and ~40% of L* galaxies host a bright satellite within the virialized extent of their dark matter halos. Remarkably, the simulation reproduces the observed frequency, radial dependence, velocity distribution, and luminosity function of observed secondaries exceptionally well, suggesting that LCDM provides an accurate reproduction of the observed Universe to galaxies as faint as L~10^9 Lsun on ~50 kpc scales. When stacked, the observed projected pairwise velocity dispersion of these satellites is sigma~160 km/s, in agreement with abundance-matching expectations for their host halo masses. Finally, bright satellites around L* primaries are significantly redder than typical galaxies in their luminosity range, indicating that environmental quenching is operating within galaxy-size dark matter halos that typically contain only a single bright satellite. This redness trend is in stark contrast to the Milky Way's LMC, which is unusually blue even for a field galaxy. We suggest that the LMC's discrepant color might be further evidence that it is undergoing a triggered star-formation event upon first infall.Comment: 14 pages, 11 figures; accepted to Ap

Stealth Galaxies in the Halo of the Milky Way

We predict that there is a population of low-luminosity dwarf galaxies orbiting within the halo of the Milky Way that have surface brightnesses low enough to have escaped detection in star-count surveys. The overall count of stealth galaxies is sensitive to the presence (or lack) of a low-mass threshold in galaxy formation. These systems have luminosities and stellar velocity dispersions that are similar to those of known ultrafaint dwarf galaxies but they have more extended stellar distributions (half light radii greater than about 100 pc) because they inhabit dark subhalos that are slightly less massive than their higher surface brightness counterparts. As a result, the typical peak surface brightness is fainter than 30 mag per square arcsec. One implication is that the inferred common mass scale for Milky Way dwarfs may be an artifact of selection bias. If there is no sharp threshold in galaxy formation at low halo mass, then ultrafaint galaxies like Segue 1 represent the high-mass, early forming tail of a much larger population of objects that could number in the hundreds and have typical peak circular velocities of about 8 km/s and masses within 300 pc of about 5 million solar masses. Alternatively, if we impose a low-mass threshold in galaxy formation in order to explain the unexpectedly high densities of the ultrafaint dwarfs, then we expect only a handful of stealth galaxies in the halo of the Milky Way. A complete census of these objects will require deeper sky surveys, 30m-class follow-up telescopes, and more refined methods to identify extended, self-bound groupings of stars in the halo.Comment: 12 pages, 7 figures, accepted by ApJ. Several crucial references added and the discussion has been expanded. Conclusions are unchanged

Are sexual media exposure, parental restrictions on media use and co-viewing TV and DVDs with parents and friends associated with teenagers' early sexual behaviour?

Sexual content in teenagers' media diets is known to predict early sexual behaviour. Research on sexual content has not allowed for the social context of media use, which may affect selection and processing of content. This study investigated whether sexual media content and/or contextual factors (co-viewing, parental media restrictions) were associated with early sexual behaviour using 2251 14–15 year-olds from Scotland, UK. A third (&lt;i&gt;n&lt;/i&gt; = 733) reported sexual intercourse. In multivariable analysis the likelihood of intercourse was lower with parental restriction of sexual media and same-sex peer co-viewing; but higher with mixed-sex peer co-viewing. Parental co-viewing, other parental restrictions on media and sexual film content exposure were not associated with intercourse. Findings suggest the context of media use may influence early sexual behaviour. Specific parental restrictions on sexual media may offer more protection against early sex than other restrictions or parental co-viewing. Further research is required to establish causal mechanisms