The research of the pulsed electron beam effect on polylactic acid scaffolds

The effect of nanosecond pulsed electron beam irradiation on properties of polylactic acid (PLLA)scaffolds was investigated. Modification of scaffolds was performed in electron beam accelerator with absorbeddose from 30 to200 kGy. Properties of the scaffolds were examined by means of XRD analysis and scanningelectron microscopy. It was shown that pulsed electron beam exposure leads to the recrystallization and changesin surface morphology

Постановка задачи для разработки ИС учета игровых подходов в обучении

Рассмотрены положительные и отрицательные аспекты обучения через онлайн-среды с применением игровых методов обучения, поставлена задача разработки

Разработка налоговой политики МУП «КРУ г.Юрги»

Объектом исследования является муниципальное унитарное предприятие "Комбинат ритуальных услуг г. Юрги". Цель работы – формирования налоговой политики муниципального унитарного предприятия "Комбинат ритуальных услуг г. Юрги". Актуальность работы определяется критическим влиянием налоговой политики на финансово-хозяйственную деятельность предприятия и повышение эффективности управления его налоговыми потоками. Для достижения поставленной цели решены следующие задачи: 1.Рассмотреть теоретическую базу проведения налогового анализа на микроуровне. 2.Выполнить анализ финансово-хозяйственной деятельности МУП КРУ г. Юрги. 3.Оценить влияние налоговых платежей на финансовый результат деятельности МУП КРУ г. Юрги. 4.Предложить рекомендации по оптимизации налоговых выплат.The purpose of the work is the formation of the tax policy of the municipal unitary enterprise "Combine of funeral services of Yurga". The relevance of the work is determined by the critical impact of tax policy on the financial and economic activities of the enterprise and improving the efficiency of managing its tax flows. To achieve this goal, the following tasks were solved: 1. Consider the theoretical basis for conducting tax analysis at the micro level. 2. To analyze the financial and economic activities of the municipal unitary enterprise of the switchgear of the city of Yurga. 3. To assess the impact of tax payments on the financial result of the activities of the municipal unitary enterprise of KRU of Yurga 4. To offer recommendations for optimizing tax payments

Влияние параметров пласта на процесс конусообразования в горизонтальных скважинах при разработке нефтегазовых месторождений

Анализ механизма проявления конусообразования и прорыва воды при разработке месторождения. Обоснование применения технологий предотвращения поступления нецелевого флюида. Комплексные решения для повышение эффективности разработки нефтегазовых залежей в условиях конусообразования.Analysis of the mechanism of manifestation of coning and water breakthrough during field development. Justification of the use of technologies to prevent the ingress of non-target fluid. Integrated solutions to improve the efficiency of oil and gas deposits development in coning conditions

Targeted Therapies in Liver Fibrosis:Combining the Best Parts of Platelet-Derived Growth Factor BB and Interferon Gamma

Cytokines, growth factors and other locally produced mediators play key roles in the regulation of disease progression. During liver fibrosis, these mediators orchestrate the balance between pro- and antifibrotic activities as exerted by the hepatic cells. Two important players in this respect are the profibrotic mediator platelet derived growth factor BB (PDGF-BB) and the antifibrotic cytokine interferon gamma (IFNγ). PDGF-BB, produced by many resident and infiltrating cells, causes extensive proliferation, migration and contraction of hepatic stellate cells (HSCs) and myofibroblasts. These cells are the extracellular matrix producing hepatic cells and they highly express the PDGFβ-receptor. On the other hand, IFNγ is produced by natural killer cells in fibrotic livers and is endowed with pro-inflammatory, antiviral and antifibrotic activities. This cytokine attracted much attention as a possible therapeutic compound in fibrosis. However, clinical trials yielded disappointing results because of low efficacy and adverse effects, most likely related to the dual role of IFNγ in fibrosis. In our studies, we targeted the antifibrotic IFNγ to the liver myofibroblasts. For that, we altered the cell binding properties of IFNγ, by delivery of the IFNγ-nuclear localization sequence to the highly expressed PDGFβ-receptor using a PDGFβ-receptor recognizing peptide, thereby creating a construct referred to as Fibroferon (i.e. fibroblast-targeted interferon γ). In recent years, we demonstrated that HSC-specific delivery of IFNγ increased its antifibrotic potency and improved its general safety profile in vivo, making Fibroferon highly suitable for the treatment of (fibrotic) diseases associated with elevated PDGFβ-receptor expression. The present review summarizes the knowledge on these two key mediators, PDGF-BB and IFNγ, and outlines how we used this knowledge to create the cell-specific antifibrotic compound Fibroferon containing parts of both of these mediators

Recombinant Expression, Purification, and Functional Characterisation of Connective Tissue Growth Factor and Nephroblastoma-Overexpressed Protein

The CCN family of proteins, especially its prominent member, the Connective tissue growth factor (CTGF/CCN2) has been identified as a possible biomarker for the diagnosis of fibrotic diseases. As a downstream mediator of TGF-β1 signalling, it is involved in tissue scarring, stimulates interstitial deposition of extracellular matrix proteins, and promotes proliferation of several cell types. Another member of this family, the Nephroblastoma-Overexpressed protein (NOV/CCN3), has growth-inhibiting properties. First reports further suggest that these two CCN family members act opposite to each other in regulating extracellular matrix protein expression and reciprocally influence their own expression when over-expressed. We have established stable HEK and Flp-In-293 clones as productive sources for recombinant human CCN2/CTGF. In addition, we generated an adenoviral vector for recombinant expression of rat NOV and established protocols to purify large quantities of these CCN proteins. The identity of purified human CCN2/CTGF and rat CCN3/NOV was proven by In-gel digest followed by ESI-TOF/MS mass spectrometry. The biological activity of purified proteins was demonstrated using a Smad3-sensitive reporter gene and BrdU proliferation assay in permanent cell line EA•hy 926 cells. We further demonstrate for the first time that both recombinant CCN proteins are N-glycosylated