50 research outputs found
Cytotoxicity and dentin composition alterations promoted by different chemomechanical caries removal agents : a preliminary in vitro study
The use of chemomechanical agents for caries removal has been indicated as a non-invasive treatment option; however, their possible deleterious effects on the dental-pulp complex have been insufficiently studied. This study assessed the direct cytotoxici
The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence
Cellular senescence is an irreversible state of cell cycle arrest occurring in response to stressful stimuli, such as telomere attrition, DNA damage, reactive oxygen species, and oncogenic proteins. Although beneficial and protective in several physiological processes, an excessive senescent cell burden has been involved in various pathological conditions including aging, tissue dysfunction and chronic diseases. Oxidative stress (OS) can drive senescence due to a loss of balance
between pro-oxidant stimuli and antioxidant defences. Therefore, the identification and characterization of antioxidant compounds capable of preventing or counteracting the senescent phenotype is of major interest. However, despite the considerable number of studies, a comprehensive overview of the main antioxidant molecules capable of counteracting OS-induced senescence is still lacking. Here, besides a brief description of the molecular mechanisms implicated in OS-mediated aging, we review and discuss the role of enzymes, mitochondria-targeting compounds, vitamins, carotenoids, organosulfur compounds, nitrogen non-protein molecules, minerals, flavonoids, and non-flavonoids as antioxidant compounds with an anti-aging potential, therefore offering insights into innovative lifespan-extending approaches
Geomagnetically trapped, albedo and solar energetic particles: trajectory analysis and flux reconstruction with PAMELA
The PAMELA satellite experiment is providing comprehensive observations of
the interplanetary and magnetospheric radiation in the near-Earth environment.
Thanks to its identification capabilities and the semi-polar orbit, PAMELA is
able to precisely measure the energetic spectra and the angular distributions
of the different cosmic-ray populations over a wide latitude region, including
geomagnetically trapped and albedo particles. Its observations comprise the
solar energetic particle events between solar cycles 23 and 24, and the
geomagnetic cutoff variations during magnetospheric storms. PAMELA's
measurements are supported by an accurate analysis of particle trajectories in
the Earth's magnetosphere based on a realistic geomagnetic field modeling,
which allows the classification of particle populations of different origin and
the investigation of the asymptotic directions of arrival.Comment: Accepted for publication in Advances in Space Research, 2016. 21
pages, 7 figure
PAMELA's measurements of geomagnetic cutoff variations during solar energetic particle events
Data from the PAMELA satellite experiment were used to measure the
geomagnetic cutoff for high-energy ( 80 MeV) protons during the solar
particle events on 2006 December 13 and 14. The variations of the cutoff
latitude as a function of rigidity were studied on relatively short timescales,
corresponding to single spacecraft orbits (about 94 minutes). Estimated cutoff
values were cross-checked with those obtained by means of a trajectory tracing
approach based on dynamical empirical modeling of the Earth's magnetosphere. We
find significant variations in the cutoff latitude, with a maximum suppression
of about 6 deg for 80 MeV protons during the main phase of the storm. The
observed reduction in the geomagnetic shielding and its temporal evolution were
compared with the changes in the magnetosphere configuration, investigating the
role of IMF, solar wind and geomagnetic (Kp, Dst and Sym-H indexes) variables
and their correlation with PAMELA cutoff results.Comment: Conference: The 34th International Cosmic Ray Conference (ICRC2015),
30 July - 6 August, 2015, The Hague, The Netherlands, Volume:
PoS(ICRC2015)28
Solar energetic particle events: trajectory analysis and flux reconstruction with PAMELA
The PAMELA satellite experiment is providing first direct measurements of
Solar Energetic Particles (SEPs) with energies from about 80 MeV to several GeV
in near-Earth space, bridging the low energy data by other space-based
instruments and the Ground Level Enhancement (GLE) data by the worldwide
network of neutron monitors. Its unique observational capabilities include the
possibility of measuring the flux angular distribution and thus investigating
possible anisotropies. This work reports the analysis methods developed to
estimate the SEP energy spectra as a function of the particle pitch-angle with
respect to the Interplanetary Magnetic Field (IMF) direction. The crucial
ingredient is provided by an accurate simulation of the asymptotic exposition
of the PAMELA apparatus, based on a realistic reconstruction of particle
trajectories in the Earth's magnetosphere. As case study, the results for the
May 17, 2012 event are presented.Comment: Conference: The 34th International Cosmic Ray Conference (ICRC2015),
30 July - 6 August, 2015, The Hague, The Netherlands, Volume:
PoS(ICRC2015)08
Rare mutations in SQSTM1 modify susceptibility to frontotemporal lobar degeneration
Mutations in the gene coding for Sequestosome 1 (SQSTM1) have been genetically associated with amyotrophic lateral sclerosis (ALS) and Paget disease of bone. In the present study, we analyzed the SQSTM1 coding sequence for mutations in an extended cohort of 1,808 patients with frontotemporal lobar degeneration (FTLD), ascertained within the European Early-Onset Dementia consortium. As control dataset, we sequenced 1,625 European control individuals and analyzed whole-exome sequence data of 2,274 German individuals (total n = 3,899). Association of rare SQSTM1 mutations was calculated in a meta-analysis of 4,332 FTLD and 10,240 control alleles. We identified 25 coding variants in FTLD patients of which 10 have not been described. Fifteen mutations were absent in the control individuals (carrier frequency < 0.00026) whilst the others were rare in both patients and control individuals. When pooling all variants with a minor allele frequency < 0.01, an overall frequency of 3.2 % was calculated in patients. Rare variant association analysis between patients and controls showed no difference over the whole protein, but suggested that rare mutations clustering in the UBA domain of SQSTM1 may influence disease susceptibility by doubling the risk for FTLD (RR = 2.18 [95 % CI 1.24-3.85]; corrected p value = 0.042). Detailed histopathology demonstrated that mutations in SQSTM1 associate with widespread neuronal and glial phospho-TDP-43 pathology. With this study, we provide further evidence for a putative role of rare mutations in SQSTM1 in the genetic etiology of FTLD and showed that, comparable to other FTLD/ALS genes, SQSTM1 mutations are associated with TDP-43 pathology
PAMELA Observation of the 2012 May 17 GLE Event
The PAMELA (Payload for Antimatter Matter Exploration and Light-nuclei Astrophysics) satellite-borne experiment has been collecting data in orbit since July 2006, providing accurate measurements of the energy spectra and composition of the cosmic radiation from a few hundred MeVn up to hundred GeVn. This wide interval of measured energies makes PAMELA a unique instrument for Solar Energetic Particle (SEP) observations. Not only does it span the energy range between the ground-based neutron monitor data and the observations of SEPs from space, but also PAMELA carries out the first direct measurements of the composition for the highest energy SEP events, including those causing Ground Level Enhancements (GLEs). PAMELA has registered many SEP events in solar cycle 24 including the 2012 May 17 GLE event (GLE 71), offering unique opportunities to address the question of high-energy SEP origin. Experimental performances and preliminary results on the 2012 May 17 events will be presented. We will discuss the derived particle injection time and compare with other time scales at the Sun including the flare and CME onset times
PAMELA's Measurements of Magnetospheric Effects on High Energy Solar Particles
The nature of particle acceleration at the Sun, whether through flare
reconnection processes or through shocks driven by coronal mass ejections
(CMEs), is still under scrutiny despite decades of research. The measured
properties of solar energetic particles (SEPs) have long been modeled in
different particle-acceleration scenarios. The challenge has been to
disentangle to the effects of transport from those of acceleration. The Payload
for Antimatter Matter Exploration and Light-nuclei Astrophysics (PAMELA)
instrument, enables unique observations of SEPs including composition and the
angular distribution of the particles about the magnetic field, i.e. pitch
angle distribution, over a broad energy range (>80 MeV) -- bridging a critical
gap between space-based measurements and ground-based. We present high-energy
SEP data from PAMELA acquired during the 2012 May 17 SEP event. These data
exhibit differential anisotropies and thus transport features over the
instrument rigidity range. SEP protons exhibit two distinct pitch angle
distributions; a low-energy population that extends to 90{\deg} and a
population that is beamed at high energies (>1 GeV), consistent with neutron
monitor measurements. To explain a low-energy SEP population that exhibits
significant scattering or redistribution accompanied by a high-energy
population that reaches the Earth relatively unaffected by dispersive transport
effects, we postulate that the scattering or redistribution takes place
locally. We believe these are the first comprehensive measurements of the
effects of solar energetic particle transport in the Earth's magnetosheath.Comment: 21 pages, 4 figures. Accepted for publication in The Astrophysical
Journal Letter
Current concepts in the prevention of pathogen transmission via blood/plasma-derived products for bleeding disorders
The pathogen safety of blood/plasma-derived products has historically been a subject of significant concern to the medical community. Measures such as donor selection and blood screening have contributed to increase the safety of these products, but pathogen transmission does still occur. Reasons for this include lack of sensitivity/specificity of current screening methods, lack of reliable screening tests for some pathogens (e.g. prions) and the fact that many potentially harmful infectious agents are not routinely screened for. Methods for the purification/inactivation of blood/plasma-derived products have been developed in order to further reduce the residual risk, but low concentrations of pathogens do not necessarily imply a low level of risk for the patient and so the overall challenge of minimising risk remains. This review aims to discuss the variable level of pathogenic risk and describes the current screening methods used to prevent/detect the presence of pathogens in blood/plasma-derived products