The Fundamental Diagram of Pedestrian Movement Revisited

The empirical relation between density and velocity of pedestrian movement is not completely analyzed, particularly with regard to the `microscopic' causes which determine the relation at medium and high densities. The simplest system for the investigation of this dependency is the normal movement of pedestrians along a line (single-file movement). This article presents experimental results for this system under laboratory conditions and discusses the following observations: The data show a linear relation between the velocity and the inverse of the density, which can be regarded as the required length of one pedestrian to move. Furthermore we compare the results for the single-file movement with literature data for the movement in a plane. This comparison shows an unexpected conformance between the fundamental diagrams, indicating that lateral interference has negligible influence on the velocity-density relation at the density domain $1 m^{-2}<\rho<5 m^{-2}$. In addition we test a procedure for automatic recording of pedestrian flow characteristics. We present preliminary results on measurement range and accuracy of this method.Comment: 13 pages, 9 figure

Enhanced empirical data for the fundamental diagram and the flow through bottlenecks

In recent years, several approaches for modelling pedestrian dynamics have been proposed and applied e.g. for design of egress routes. However, so far not much attention has been paid to their 'quantitative' validation. This unsatisfactory situation belongs amongst others on the uncertain and contradictory experimental data base. The fundamental diagram, i.e. the density-dependence of the flow or velocity, is probably the most important relation as it connects the basic parameter to describe the dynamic of crowds. But specifications in different handbooks as well as experimental measurements differ considerably. The same is true for the bottleneck flow. After a comprehensive review of the experimental data base we give an survey of a research project, including experiments with up to 250 persons performed under well controlled laboratory conditions. The trajectories of each person are measured in high precision to analyze the fundamental diagram and the flow through bottlenecks. The trajectories allow to study how the way of measurement influences the resulting relations. Surprisingly we found large deviation amongst the methods. These may be responsible for the deviation in the literature mentioned above. The results are of particular importance for the comparison of experimental data gained in different contexts and for the validation of models.Comment: A contribution to: Pedestrian and Evacuation Dynamics 2008 (Springer) 12 pages, 7 figure

Sporadic Creutzfeldt-Jakob disease VM1: phenotypic and molecular characterization of a novel subtype of human prion disease

The methionine (M)-valine (V) polymorphic codon 129 of the prion protein gene (PRNP) plays a central role in both susceptibility and phenotypic expression of sporadic Creutzfeldt-Jakob diseases (sCJD). Experimental transmissions of sCJD in humanized transgenic mice led to the isolation of five prion strains, named M1, M2C, M2T, V2, and V1, based on two major conformations of the pathological prion protein (PrPSc, type 1 and type 2), and the codon 129 genotype determining susceptibility and propagation efficiency. While the most frequent sCJD strains have been described in codon 129 homozygosis (MM1, MM2C, VV2) and heterozygosis (MV1, MV2K, and MV2C), the V1 strain has only been found in patients carrying VV. We identified six sCJD cases, 4 in Catalonia and 2 in Italy, carrying MV at PRNP codon 129 in combination with PrPSc type 1 and a new clinical and neuropathological profile reminiscent of the VV1 sCJD subtype rather than typical MM1/MV1. All patients had a relatively long duration (mean of 20.5 vs. 3.5 months of MM1/MV1 patients) and lacked electroencephalographic periodic sharp-wave complexes at diagnosis. Distinctive histopathological features included the spongiform change with vacuoles of larger size than those seen in sCJD MM1/MV1, the lesion profile with prominent cortical and striatal involvement, and the pattern of PrPSc deposition characterized by a dissociation between florid spongiform change and mild synaptic deposits associated with coarse, patch-like deposits in the cerebellar molecular layer. Western blot analysis of brain homogenates revealed a PrPSc type 1 profile with physicochemical properties reminiscent of the type 1 protein linked to the VV1 sCJD subtype. In summary, we have identified a new subtype of sCJD with distinctive clinicopathological features significantly overlapping with those of the VV1 subtype, possibly representing the missing evidence of V1 sCJD strain propagation in the 129MV host genotype

A glossary for research on human crowd dynamics

This article presents a glossary of terms that are frequently used in research on human crowds. This topic is inherently multidisciplinary as it includes work in and across computer science, engineering, mathematics, physics, psychology and social science, for example. We do not view the glossary presented here as a collection of finalised and formal definitions. Instead, we suggest it is a snapshot of current views and the starting point of an ongoing process that we hope will be useful in providing some guidance on the use of terminology to develop a mutual understanding across disciplines. The glossary was developed collaboratively during a multidisciplinary meeting. We deliberately allow several definitions of terms, to reflect the confluence of disciplines in the field. This also reflects the fact not all contributors necessarily agree with all definitions in this glossary

Structural insights into cardiolipin transfer from the Inner membrane to the outer membrane by PbgA in Gram-negative bacteria

The outer membrane (OM) of Gram-negative bacteria is a unique asymmetric lipid bilayer in which the outer leaflet is composed of lipopolysaccharide (LPS) and the inner leaflet is formed by glycerophospholipid (GPL). The OM plays a fundamental role in protecting Gram-negative bacteria from harsh environments and toxic compounds. The transport and assembly pathways for phospholipids of bacterial OM are unknown. Cardiolipin (CL) plays an important role in OM biogenesis and pathogenesis, and the inner membrane (IM) protein PbgA, containing five transmembrane domains and a globular domain in periplasm has been recently identified as a CL transporter from the IM to the OM with an unknown mechanism. Here we present the first two crystal structures of soluble periplasmic globular domain of PbgA from S. typhimurium and E. coli, which revealed that the globular domains of PbgA resemble the structures of the arylsulfatase protein family and contains a novel core hydrophobic pocket that may be responsible for binding and transporting CLs. Our structural and functional studies shed an important light on the mechanism of CL transport in Gram-negative bacteria from the IM to the OM, which offers great potential for the development of novel antibiotics against multi-drug resistant bacterial infections

Antimicrobial activity of cobalt imidazolate metal-organic frameworks

INGENIERIE+SAG:JEC:DFATwo cobalt imidazolate metal-organic frameworks were evaluated as a bactericidal material against the growth of the Gram-negative bacteria Pseudomonas putida and Escherichia coil. Under the most unfavourable conditions, within the exponential growth phase and in the culture media for both microorganisms, the growth inhibition reached over 50% for concentrations of biocidal material in the 5(-10) mg L-1 range. The release of metal gives excellent durability with the antibacterial effect persisting after 3 months. Both cobalt-based materials can be prepared with simple, cheap and easily accessible commercial ligands, leading to a more affordable possible future application as antimicrobial materials. (C) 2014 Elsevier Ltd. All rights reserved

Bidirectional Flow on Stairs at Different Flow Ratios

Bidirectional flow happens not only on horizontal planes, but also on stairs. So far, bidirectional horizontal movements have been investigated through various field studies and controlled experiments, but such movements on stairs are rarely paid attention to, especially those based on trajectories. In this paper we investigate bidirectional flow on stairs at different flow ratios ranging from 0.1 to 0.9. Preliminary results such as lane formation, crossing time and fundamental diagrams in three movement scenarios are presented, and the characteristics of the descending and ascending pedestrians are compared. Our results can be applied for the validation and calibration of simulation models on stairs