71 research outputs found
Differences Between The Optical/Uv Spectra Of X-Ray Bright And X-Ray Faint QSOs
We contrast measurements of composite optical and ultraviolet (UV) spectra
constructed from samples of QSOs defined by their soft X-ray brightness. X-ray
bright (XB) composites show stronger emission lines in general, but
particularly from the narrow line region. The difference in the [OIII]/Hbeta
ratio is particularly striking, and even more so when blended FeII emission is
properly subtracted. The correlation of this ratio with X-ray brightness were
principal components of QSO spectral diversity found by Boroson & Green (1992).
We find here that other, much weaker narrow optical forbidden lines ([OII] and
NeV) are enhanced by factors of 2 to 3 in our XB composites, and that narrow
line emission is also strongly enhanced in the XB UV composite. Broad permitted
line fluxes are slightly larger for all XB spectra, but the narrow/broad line
ratio stays similar or increases strongly with X-ray brightness for all strong
permitted lines except Hbeta.
Spectral differences between samples divided by their relative X-ray
brightness (as measured by alpha_{ox}) exceed those seen between complementary
samples divided by luminosity or radio loudness. We propose that the Baldwin
effect may be a secondary correlation to the primary relationship between
alpha_{ox} and emission line equivalent width. We conclude that either 1)
equivalent width depends strongly on the SHAPE of the ionizing continuum, as
crudely characterized here by alpha_{ox} or 2) both equivalent width and
alpha_{ox} are related to some third parameter characterizing the QSO physics.
One such possibility is intrinsic warm absorption; a soft X-ray absorber
situated between the broad and narrow line regions can successfully account for
many of the properties observed.Comment: 16 pages including 3 figures, AAS latex, plus 4 tables totaling 5
pages, to appear in ApJ Vol. 498, May 1, 199
High-Redshift SDSS Quasars with Weak Emission Lines
We identify a sample of 74 high-redshift quasars (z>3) with weak emission
lines from the Fifth Data Release of the Sloan Digital Sky Survey and present
infrared, optical, and radio observations of a subsample of four objects at
z>4. These weak emission-line quasars (WLQs) constitute a prominent tail of the
Lya+NV equivalent width distribution, and we compare them to quasars with more
typical emission-line properties and to low-redshift active galactic nuclei
with weak/absent emission lines, namely BL Lac objects. We find that WLQs
exhibit hot (T~1000 K) thermal dust emission and have rest-frame 0.1-5 micron
spectral energy distributions that are quite similar to those of normal
quasars. The variability, polarization, and radio properties of WLQs are also
different from those of BL Lacs, making continuum boosting by a relativistic
jet an unlikely physical interpretation. The most probable scenario for WLQs
involves broad-line region properties that are physically distinct from those
of normal quasars.Comment: Updated to match version published in ApJ. 20 pages, 12 figure
Functionally Redundant RXLR Effectors from <em>Phytophthora infestans</em> Act at Different Steps to Suppress Early flg22-Triggered Immunity
Genome sequences of several economically important phytopathogenic oomycetes have revealed the presence of large families of so-called RXLR effectors. Functional screens have identified RXLR effector repertoires that either compromise or induce plant defense responses. However, limited information is available about the molecular mechanisms underlying the modes of action of these effectors in planta. The perception of highly conserved pathogen- or microbe-associated molecular patterns (PAMPs/MAMPs), such as flg22, triggers converging signaling pathways recruiting MAP kinase cascades and inducing transcriptional re-programming, yielding a generic anti-microbial response. We used a highly synchronizable, pathogen-free protoplast-based assay to identify a set of RXLR effectors from Phytophthora infestans (PiRXLRs), the causal agent of potato and tomato light blight that manipulate early stages of flg22-triggered signaling. Of thirty-three tested PiRXLR effector candidates, eight, called Suppressor of early Flg22-induced Immune response (SFI), significantly suppressed flg22-dependent activation of a reporter gene under control of a typical MAMP-inducible promoter (pFRK1-Luc) in tomato protoplasts. We extended our analysis to Arabidopsis thaliana, a non-host plant species of P. infestans. From the aforementioned eight SFI effectors, three appeared to share similar functions in both Arabidopsis and tomato by suppressing transcriptional activation of flg22-induced marker genes downstream of post-translational MAP kinase activation. A further three effectors interfere with MAMP signaling at, or upstream of, the MAP kinase cascade in tomato, but not in Arabidopsis. Transient expression of the SFI effectors in Nicotiana benthamiana enhances susceptibility to P. infestans and, for the most potent effector, SFI1, nuclear localization is required for both suppression of MAMP signaling and virulence function. The present study provides a framework to decipher the molecular mechanisms underlying the manipulation of host MAMP-triggered immunity (MTI) by P. infestans and to understand the basis of host versus non-host resistance in plants towards P. infestans
The Genome of Deep-Sea Vent Chemolithoautotroph Thiomicrospira crunogena XCL-2
Presented here is the complete genome sequence of Thiomicrospira crunogena XCL-2, representative of ubiquitous chemolithoautotrophic sulfur-oxidizing bacteria isolated from deep-sea hydrothermal vents. This gammaproteobacterium has a single chromosome (2,427,734 base pairs), and its genome illustrates many of the adaptations that have enabled it to thrive at vents globally. It has 14 methyl-accepting chemotaxis protein genes, including four that may assist in positioning it in the redoxcline. A relative abundance of coding sequences (CDSs) encoding regulatory proteins likely control the expression of genes encoding carboxysomes, multiple dissolved inorganic nitrogen and phosphate transporters, as well as a phosphonate operon, which provide this species with a variety of options for acquiring these substrates from the environment. Thiom. crunogena XCL-2 is unusual among obligate sulfur-oxidizing bacteria in relying on the Sox system for the oxidation of reduced sulfur compounds. The genome has characteristics consistent with an obligately chemolithoautotrophic lifestyle, including few transporters predicted to have organic allocrits, and Calvin-Benson-Bassham cycle CDSs scattered throughout the genome
A Full Year's Chandra Exposure on SDSS Quasars from the Chandra Multiwavelength Project
We study the spectral energy distributions and evolution of a large sample of
optically selected quasars from the Sloan Digital Sky Survey (SDSS) that were
observed in 323 Chandra images analyzed by the Chandra Multiwavelength Project
(ChaMP). Our highest-confidence matched sample includes 1135 X-ray detected
quasars in the redshift range 0.2<z<5.4, representing some 36Msec of effective
exposure. Spectroscopic redshifts are available for about 1/3 of the detected
sample; elsewhere, redshifts are estimated photometrically. With 56 z>3 QSOs
detected, we find no evidence for evolution out to z~5 for either the X-ray
photon index Gamma or for the ratio of optical/UV to X-ray flux alpha_ox. About
10% of detected QSOs are obscured (Nh>1E22), but the fraction might reach ~1/3
if most non-detections are absorbed. We confirm a significant correlation
between alpha_ox and optical luminosity, but it flattens or disappears for
fainter AGN alone. Gamma hardens significantly both towards higher X-ray
luminosity, and for relatively X-ray loud quasars. These trends may represent a
relative increase in non-thermal X-ray emission, and our findings thereby
strengthen analogies between Galactic black hole binaries and AGN.Comment: 28 pages, 21 figures. Accepted (26 Aug 2008) for publication in ApJS.
Electronic datafiles (for tables 2 and 3) and high resolution figures
available at http://hea-www.harvard.edu/CHAMP
The Arabidopsis protein phosphatase PP2C38 negatively regulates the central immune kinase BIK1
Plants recognize pathogen-associated molecular patterns (PAMPs) via cell surface-localized pattern recognition receptors (PRRs), leading to PRR-triggered immunity (PTI). The Arabidopsis cytoplasmic kinase BIK1 is a downstream substrate of several PRR complexes. How plant PTI is negatively regulated is not fully understood. Here, we identify the protein phosphatase PP2C38 as a negative regulator of BIK1 activity and BIK1-mediated immunity. PP2C38 dynamically associates with BIK1, as well as with the PRRs FLS2 and EFR, but not with the co-receptor BAK1. PP2C38 regulates PAMP-induced BIK1 phosphorylation and impairs the phosphorylation of the NADPH oxidase RBOHD by BIK1, leading to reduced oxidative burst and stomatal immunity. Upon PAMP perception, PP2C38 is phosphorylated on serine 77 and dissociates from the FLS2/EFR-BIK1 complexes, enabling full BIK1 activation. Together with our recent work on the control of BIK1 turnover, this study reveals another important regulatory mechanism of this central immune component
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The Genome of Deep-Sea Vent Chemolithoautotroph Thiomicrospira crunogena XCL-2
Presented here is the complete genome sequence of Thiomicrospira crunogena XCL-2, representative of ubiquitous chemolithoautotrophic sulfur-oxidizing bacteria isolated from deep-sea hydrothermal vents. This gammaproteobacterium has a single chromosome (2,427,734 bp), and its genome illustrates many of the adaptations that have enabled it to thrive at vents globally. It has 14 methyl-accepting chemotaxis protein genes, including four that may assist in positioning it in the redoxcline. A relative abundance of CDSs encoding regulatory proteins likely control the expression of genes encoding carboxysomes, multiple dissolved inorganic nitrogen and phosphate transporters, as well as a phosphonate operon, which provide this species with a variety of options for acquiring these substrates from the environment. T. crunogena XCL-2 is unusual among obligate sulfur oxidizing bacteria in relying on the Sox system for the oxidation of reduced sulfur compounds. A 38 kb prophage is present, and a high level of prophage induction was observed, which may play a role in keeping competing populations of close relatives in check. The genome has characteristics consistent with an obligately chemolithoautotrophic lifestyle, including few transporters predicted to have organic allocrits, and Calvin-Benson-Bassham cycle CDSs scattered throughout the genome
A global experiment on motivating social distancing during the COVID-19 pandemic
Finding communication strategies that effectively motivate social distancing continues to be a global public health priority during the COVID-19 pandemic. This cross-country, preregistered experiment (n = 25,718 from 89 countries) tested hypotheses concerning generalizable positive and negative outcomes of social distancing messages that promoted personal agency and reflective choices (i.e., an autonomy-supportive message) or were restrictive and shaming (i.e., a controlling message) compared with no message at all. Results partially supported experimental hypotheses in that the controlling message increased controlled motivation (a poorly internalized form of motivation relying on shame, guilt, and fear of social consequences) relative to no message. On the other hand, the autonomy-supportive message lowered feelings of defiance compared with the controlling message, but the controlling message did not differ from receiving no message at all. Unexpectedly, messages did not influence autonomous motivation (a highly internalized form of motivation relying on one’s core values) or behavioral intentions. Results supported hypothesized associations between people’s existing autonomous and controlled motivations and self-reported behavioral intentions to engage in social distancing. Controlled motivation was associated with more defiance and less long-term behavioral intention to engage in social distancing, whereas autonomous motivation was associated with less defiance and more short- and long-term intentions to social distance. Overall, this work highlights the potential harm of using shaming and pressuring language in public health communication, with implications for the current and future global health challenges
A global experiment on motivating social distancing during the COVID-19 pandemic
Finding communication strategies that effectively motivate social distancing continues to be a global public health priority during the COVID-19 pandemic. This cross-country, preregistered experiment (n = 25,718 from 89 countries) tested hypotheses concerning generalizable positive and negative outcomes of social distancing messages that promoted personal agency and reflective choices (i.e., an autonomy-supportive message) or were restrictive and shaming (i.e. a controlling message) compared to no message at all. Results partially supported experimental hypotheses in that the controlling message increased controlled motivation (a poorly-internalized form of motivation relying on shame, guilt, and fear of social consequences) relative to no message. On the other hand, the autonomy-supportive message lowered feelings of defiance compared to the controlling message, but the controlling message did not differ from receiving no message at all. Unexpectedly, messages did not influence autonomous motivation (a highly-internalized form of motivation relying on one’s core values) or behavioral intentions. Results supported hypothesized associations between people’s existing autonomous and controlled motivations and self-reported behavioral intentions to engage in social distancing: Controlled motivation was associated with more defiance and less long-term behavioral intentions to engage in social distancing, whereas autonomous motivation was associated with less defiance and more short- and long-term intentions to social distance. Overall, this work highlights the potential harm of using shaming and pressuring language in public health communication, with implications for the current and future global health challenges
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