San Vincenzo, Isola di Stromboli (Lipari, Prov. Di Messina) - Campagna 2014

Presentazione sintetica dei risultati della campagne di scavo effettuate nel 2014 nel sito archeologico di San Vincenzo a Strombol

Italo-Mycenaean and other Aegean-influenced pottery in Late Bronze Age Italy: the case for regional production

Decorated Italo-Mycenaean (IM) pottery, a high-status class found and made over three centuries from the Italian Late Middle Bronze Age onwards, was the subject of a large archaeological and archaeometric enquiry published by the present authors in 2014. The present paper focuses on identifying IM’s centres of production. The results of chemical analysis of IM using mainly ICP-ES make a strong case for regional production, irrespective of findspots in several parts of Italy. This accords well with the relative stylistic individuality of IM observed among the finds of IM across many parts of Italy, suggesting that IM is a powerful archaeological indicator of the way local communities were constructing and negotiating their identities at this crucial time of social and economic change at the end of the Bronze Age. A picture of more dispersed intra-regional production emerges from the combined chemical and petrographic analysis of two other pottery classes displaying Aegean influence: wheel-made Grey ware and decorated Final Bronze Age/Early Iron Age (FBA/EIA) pottery from sites in present-day Apulia and from Broglio di Trebisacce in Calabria. Potters manufacturing the former applied their knowledge of the wheel and kiln firing to handmade impasto shapes which were largely shared by local communities within a region. The results obtained for the latter reflect demands of the new elites of the emerging FBA/EIA in southern Italy to create symbols expressing a new cultural identity: this pottery’s style, especially of Protogeometric, was uniform but its production was localised

Comprehension of double-center embedded relatives in Italian: a case for hierarchical intervention

Object relatives are more difficult to process than subject relatives. Several sentence processing models have been proposed to explain this difference. As double-center embedding relatives contain several long-distance dependencies, they are an ideal configuration to compare sentence processing models. The main aim of the present study was to compare the predictions of the featural Relativized Minimality approach with the ones of other relevant sentence processing models. 57 Italian-speaking healthy adults answered comprehension questions concerning the first, second, or third verb to appear in both double-center embedding and control sentences. Results show that questions concerning the matrix verb of double-center embedding structures were significantly easier and were associated with faster response times than questions concerning the embedded verbs. Furthermore, in object double-center embedding relatives the questions concerning the verb of the most embedded clause were easier than the ones concerning the verb of the intermediate embedded clause. This pattern of results is consistent with featural Relativized Minimality but cannot be fully explained by other sentence processing models

Quick assessment of cell-free DNA in seminal fluid and fragment size for early non-invasive prostate cancer diagnosis

Liquid biopsy consists in the quantification and qualification of circulating cell-free DNA (cfDNA) and tumor-derived DNA (ctDNA) for cancer recognition. Recently, the characterization of seminal cfDNA (scfDNA) has been reported as a possible biomarker for prostate cancer (PCa) diagnosis

Integrated frequency comb source of heralded single photons

We report an integrated photon pair source based on a CMOS-compatible microring resonator that generates multiple, simultaneous, and independent photon pairs at different wavelengths in a frequency comb compatible with fiber communication wavelength division multiplexing channels (200 GHz channel separation) and with a linewidth that is compatible with quantum memories (110 MHz). It operates in a self-locked pump configuration, avoiding the need for active stabilization, making it extremely robust even at very low power levels

Functional Characterization of Aquaporin-4 Specific T Cells: Towards a Model for Neuromyelitis Optica

Antibodies to the water channel protein aquaporin-4 (AQP4), which is expressed in astrocytic endfeet at the blood brain barrier, have been identified in the serum of Neuromyelitis optica (NMO) patients and are believed to induce damage to astrocytes. However, AQP4 specific T helper cell responses that are required for the generation of anti-AQP4 antibodies and most likely also for the formation of intraparenchymal CNS lesions have not been characterized. specific T cells were present in the natural T cell repertoire of wild type C57BL/6 mice and T cell lines were raised. However, active immunization with these AQP4 peptides did not induce signs of spinal cord disease. Rather, sensitization with AQP4 peptides resulted in production of IFN-γ, but also IL-5 and IL-10 by antigen-specific T cells. Consistent with this cytokine profile, the AQP4 specific antibody response upon immunization with full length AQP4 included IgG1 and IgG2, which are associated with a mixed Th2/Th1 T cell response. restricted AQP4 specific T cell epitopes will allow us to investigate how AQP4 specific autoimmune reactions are regulated and to establish faithful mouse models of NMO that include both cellular and humoral responses against AQP4

TREM2-Transduced Myeloid Precursors Mediate Nervous Tissue Debris Clearance and Facilitate Recovery in an Animal Model of Multiple Sclerosis

BACKGROUND: In multiple sclerosis, inflammation can successfully be prevented, while promoting repair is still a major challenge. Microglial cells, the resident phagocytes of the central nervous system (CNS), are hematopoietic-derived myeloid cells and express the triggering receptor expressed on myeloid cells 2 (TREM2), an innate immune receptor. Myeloid cells are an accessible source for ex vivo gene therapy. We investigated whether myeloid precursor cells genetically modified to express TREM2 affect the disease course of experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis. METHODS AND FINDINGS: EAE was induced in mice by immunization with a myelin autoantigen. Intravenous application of TREM2-transduced bone marrow–derived myeloid precursor cells at the EAE peak led to an amelioration of clinical symptoms, reduction in axonal damage, and prevention of further demyelination. TREM2-transduced myeloid cells applied intravenously migrated into the inflammatory spinal cord lesions of EAE-diseased mice, showed increased lysosomal and phagocytic activity, cleared degenerated myelin, and created an anti-inflammatory cytokine milieu within the CNS. CONCLUSIONS: Intravenously applied bone marrow–derived and TREM2-tranduced myeloid precursor cells limit tissue destruction and facilitate repair within the murine CNS by clearance of cellular debris during EAE. TREM2 is a new attractive target for promotion of repair and resolution of inflammation in multiple sclerosis and other neuroinflammatory diseases

Increased CD8+ T cell responses to apoptotic T cell-associated antigens in multiple sclerosis.

BACKGROUND: Here, we evaluated the hypothesis that CD8(+) T cell responses to caspase-cleaved antigens derived from effector T cells undergoing apoptosis, may contribute to multiple sclerosis (MS) immunopathology. METHODS: The percentage of autoreactive CD8(+) T effector cells specific for various apoptotic T cell-associated self-epitopes (apoptotic epitopes) were detected in the peripheral blood and cerebrospinal fluid (CSF) by both enzyme-linked immunospot and dextramers of class I molecules complexed with relevant apoptotic epitopes. Moreover, the capacity of dextramer(+) CD8(+) T cells to produce interferon (IFN)-γ and/or interleukin (IL)-17 in response to the relevant apoptotic epitopes was evaluated by the intracellular cytokine staining. Cross-presentation assay of apoptotic T cells by dendritic cells was also evaluated ex vivo. RESULTS: We found that polyfunctional (IFN-γ and/or IL-17 producing) autoreactive CD8(+) T cells specific for apoptotic epitopes were represented in MS patients with frequencies significantly higher than in healthy donors. These autoreactive CD8(+) T cells with a strong potential to produce IFN-γ or IL-17 in response to the relevant apoptotic epitopes were significantly accumulated in the CSF from the same patients. In addition, the frequencies of these autoreactive CD8(+) T cells correlated with the disease disability. Cross-presentation assay revealed that caspase-cleaved cellular proteins are required to activate apoptotic epitope-specific CD8(+) T cells ex vivo. CONCLUSION: Taken together, these data indicate that apoptotic epitope-specific CD8(+) T cells with strong inflammatory potential are recruited at the level of the inflammatory site, where they may be involved in MS immunopathology through the production of high levels of inflammatory cytokines