1,744 research outputs found
West Asian sources of the Eurasian component in Ethiopians: a reassessment
The presence of genomic signatures of Eurasian origin in contemporary Ethiopians has been reported by several authors and estimated to have arrived in the area from 3000 years ago. Several studies reported plausible source populations for such a signature, using haplotype based methods on modern data or single-site methods on modern or ancient data. These studies did not reach a consensus and suggested an Anatolian or Sardinia-like proxy, broadly Levantine or Neolithic Levantine as possible sources. We demonstrate, however, that the deeply divergent, autochthonous African component which accounts for ~50% of most contemporary Ethiopian genomes, affects the overall allele frequency spectrum to an extent that makes it hard to control for it and, at once, to discern between subtly different, yet important, Eurasian sources (such as Anatolian or Levant Neolithic ones). Here we re-assess pattern of allele sharing between the Eurasian component of Ethiopians (here called “NAF” for Non African) and ancient and modern proxies. Our results unveil a genomic legacy that may connect the Eurasian genetic component of contemporary Ethiopians with Sea People and with population movements that affected the Mediterranean area and the Levant after the fall of the Minoan civilization
Mapping the bathymetry of supraglacial lakes and streams on the Greenland Ice Sheet using field measurements and high resolution satellite images
Recent melt events on the Greenland ice sheet (GrIS) accentuate the need to constrain estimates of sea level rise through improved characterization of meltwater pathways. This effort will require more precise estimates of the volume of water stored on the surface of the GrIS. We assessed the potential to obtain such information by mapping the bathymetry of supraglacial lakes and streams from WorldView2 (WV2) satellite images. Simultaneous in situ observations of depth and reflectance from two streams and a lake with measured depths up to 10.45 m were used to test a spectrally based depth retrieval algorithm. We performed optimal band ratio analysis (OBRA) of continuous field spectra and spectra convolved to the bands of the WV2, Landsat 7 (ETM+), MODIS, and ASTER sensors. The field spectra yielded a strong relationship with depth (R² = 0.94), and OBRA R² values were nearly as high (0.87–0.92) for convolved spectra, suggesting that these sensors' broader bands would be sufficient for depth retrieval. Our field measurements thus indicated that remote sensing of supraglacial bathymetry is not only feasible but potentially highly accurate. OBRA of spectra from 2 m-pixel WV2 images acquired within 3–72 h of our field observations produced an optimal R² value of 0.92 and unbiased, precise depth estimates, with mean and root mean square errors < 1% and 10–25% of the mean depth. Bathymetric maps produced by applying OBRA relations revealed subtle features of lake and channel morphology. In addition to providing refined storage volume estimates for lakes of various sizes, this approach can help provide estimates of the transient flux of meltwater through stream
The activation of the atypical PKC zeta in light-induced retinal degeneration and its involvement in L-DNase II control.
Light-induced retinal degeneration is characterized by photoreceptor cell death. Many studies showed that photoreceptor demise is caspase-independent. In our laboratory we showed that leucocyte elastase inhibitor/LEI-derived DNase II (LEI/L-DNase II), a caspase-independent apoptotic pathway, is responsible for photoreceptor death. In this work, we investigated the activation of a pro-survival kinase, the protein kinase C (PKC) zeta. We show that light exposure induced PKC zeta activation. PKC zeta interacts with LEI/L-DNase II and controls its DNase activity by impairing its nuclear translocation. These results highlight the role of PKC zeta in retinal physiology and show that this kinase can control caspase-independent pathways
Anatomy of the AGN in NGC 5548: V. A clear view of the X-ray narrow emission lines
Context. Our consortium performed an extensive multi-wavelength campaign of
the nearby Seyfert 1 galaxy NGC 5548 in 2013-14. The source appeared unusually
heavily absorbed in the soft X-rays, and signatures of outflowing absorption
were also present in the UV. He-like triplets of neon, oxygen and nitrogen, and
radiative recombination continuum (RRC) features were found to dominate the
soft X-ray spectrum due to the low continuum flux.
Aims. Here we focus on characterising these narrow emission features using
data obtained from the XMM-Newton RGS (770 ks stacked spectrum).
Methods. We use SPEX for our initial analysis of these features.
Self-consistent photoionisation models from Cloudy are then compared with the
data to characterise the physical conditions of the emitting region.
Results. Outflow velocity discrepancies within the O VII triplet lines can be
explained if the X-ray narrow-line region (NLR) in NGC 5548 is absorbed by at
least one of the six warm absorber components found by previous analyses. The
RRCs allow us to directly calculate a temperature of the emitting gas of a few
eV ( K), favouring photoionised conditions. We fit the data with a
Cloudy model of log erg cm s, log cm and log v km s for the emitting
gas; this is the first time the X-ray NLR gas in this source has been modelled
so comprehensively. This allows us to estimate the distance from the central
source to the illuminated face of the emitting clouds as pc,
consistent with previous work.Comment: Accepted by A&A, 15 pages, 6 figure
The outer limiting membrane (OLM) revisited: clinical implications
PURPOSE: The outer limiting membrane (OLM) is considered to play a role in maintaining the structure of the retina through mechanical strength. However, the observation of junction proteins located at the OLM and its barrier permeability properties may suggest that the OLM may be part of the retinal barrier.
MATERIAL AND METHODS: Normal and diabetic rat, monkey, and human retinas were used to analyze junction proteins at the OLM. Proteome analyses were performed using immunohistochemistry on sections and flat-mounted retinas and western blotting on protein extracts obtained from laser microdissection of the photoreceptor layers. Semi-thin and ultrastructure analyses were also reported.
RESULTS: In the rat retina, in the subapical region zonula occludens-1 (ZO-1), junction adhesion molecule (JAM), an atypical protein kinase C, is present and the OLM shows dense labeling of occludin, JAM, and ZO-1. The presence of occludin has been confirmed using western blot analysis of the microdissected OLM region. In diabetic rats, occludin expression is decreased and glial cells junctions are dissociated. In the monkey retina, occludin, JAM, and ZO-1 are also found in the OLM. Junction proteins have a specific distribution around cone photoreceptors and Müller glia. Ultrastructural analyses suggest that structures like tight junctions may exist between retinal glial Müller cells and photoreceptors.
CONCLUSIONS: In the OLM, heterotypic junctions contain proteins from both adherent and tight junctions. Their structure suggests that tight junctions may exist in the OLM. Occludin is present in the OLM of the rat and monkey retina and it is decreased in diabetes. The OLM should be considered as part of the retinal barrier that can be disrupted in pathological conditions contributing to fluid accumulation in the macula
The Chandra X-ray Survey of Planetary Nebulae (ChanPlaNS): Probing Binarity, Magnetic Fields, and Wind Collisions
We present an overview of the initial results from the Chandra Planetary
Nebula Survey (ChanPlaNS), the first systematic (volume-limited) Chandra X-ray
Observatory survey of planetary nebulae (PNe) in the solar neighborhood. The
first phase of ChanPlaNS targeted 21 mostly high-excitation PNe within ~1.5 kpc
of Earth, yielding 4 detections of diffuse X-ray emission and 9 detections of
X-ray-luminous point sources at the central stars (CSPNe) of these objects.
Combining these results with those obtained from Chandra archival data for all
(14) other PNe within ~1.5 kpc that have been observed to date, we find an
overall X-ray detection rate of ~70%. Roughly 50% of the PNe observed by
Chandra harbor X-ray-luminous CSPNe, while soft, diffuse X-ray emission tracing
shocks formed by energetic wind collisions is detected in ~30%; five objects
display both diffuse and point-like emission components. The presence of X-ray
sources appears correlated with PN density structure, in that molecule-poor,
elliptical nebulae are more likely to display X-ray emission (either point-like
or diffuse) than molecule-rich, bipolar or Ring-like nebulae. All but one of
the X-ray point sources detected at CSPNe display X-ray spectra that are harder
than expected from hot (~100 kK) central star photospheres, possibly indicating
a high frequency of binary companions to CSPNe. Other potential explanations
include self-shocking winds or PN mass fallback. Most PNe detected as diffuse
X-ray sources are elliptical nebulae that display a nested shell/halo structure
and bright ansae; the diffuse X-ray emission regions are confined within inner,
sharp-rimmed shells. All sample PNe that display diffuse X-ray emission have
inner shell dynamical ages <~5x10^3 yr, placing firm constraints on the
timescale for strong shocks due to wind interactions in PNe.Comment: 41 pages, 6 figures; submitted to the Astronomical Journa
X-ray Characteristics of NGC 3516: A View through the Complex Absorber
We consider new Suzaku data for NGC 3516 taken during 2009, along with other
recent X-ray observations of the source. The cumulative characteristics of NGC
3516 cannot be explained without invoking changes in the line-of-sight
absorption. Contrary to many other well-studied Seyfert galaxies, NGC 3516 does
not show a positive lag of hard X-ray photons relative to soft photons over the
timescales sampled. In the context of reverberation models for the X-ray lags,
the lack of such a signal in NGC 3516 is consistent with flux variations being
dominated by absorption changes. The lack of any reverberation signal in such a
highly variable source disfavors intrinsic continuum variability in this case.
Instead, the colorless flux variations observed at high flux states for NGC
3516 are suggested to be a consequence of Compton-thick clumps of gas crossing
the line-of-sight.Comment: 12 pages, 6 figures, accepted for publication in The Astrophysical
Journa
Adaptive response and enlargement of dynamic range
Many membrane channels and receptors exhibit adaptive, or desensitized,
response to a strong sustained input stimulus, often supported by protein
activity-dependent inactivation. Adaptive response is thought to be related to
various cellular functions such as homeostasis and enlargement of dynamic range
by background compensation. Here we study the quantitative relation between
adaptive response and background compensation within a modeling framework. We
show that any particular type of adaptive response is neither sufficient nor
necessary for adaptive enlargement of dynamic range. In particular a precise
adaptive response, where system activity is maintained at a constant level at
steady state, does not ensure a large dynamic range neither in input signal nor
in system output. A general mechanism for input dynamic range enlargement can
come about from the activity-dependent modulation of protein responsiveness by
multiple biochemical modification, regardless of the type of adaptive response
it induces. Therefore hierarchical biochemical processes such as methylation
and phosphorylation are natural candidates to induce this property in signaling
systems.Comment: Corrected typos, minor text revision
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