30 research outputs found
Dependability for the Internet of Things—from dependable networking in harsh environments to a holistic view on dependability
Get PDFElectrochemically controlled self-assembled monolayers characterized with molecular and sub-molecular resolution
Get PDFA community resource for paired genomic and metabolomic data mining
Get PDFGenomics and metabolomics are widely used to explore specialized metabolite diversity. The Paired Omics Data Platform is a community initiative to systematically document links between metabolome and (meta)genome data, aiding identification of natural product biosynthetic origins and metabolite structures.Peer reviewe
Die Umgestaltung der Unternehmenslandschaft Ostdeutschlands - eine Analyse des Transformationsprozesses der Jahre 1990 bis 1994 unter regionalen Aspekten
No full textDie Neugestaltung der Unternehmenslandschaft in den neuen Bundesländern. Eine regionalwissenschaftliche Analyse der ersten "Nach-Wende-Jahre"
No full textHeterologous expression of the cryptic mdk gene cluster and structural revision of maduralactomycin A
No full textAbstract: After conducting an in silico analysis of the cryptic mdk cluster region and performing transcriptomic studies, an integrative Streptomyces BAC Vector containing the mdk gene sequence was constructed. The heterologous expression of the mdk cluster in Streptomyces albus J1074 resulted in the production of the angucyclic product, seongomycin, which allowed for the assesment of its antibacterial, antiproliferative, and antiviral activities. Heterologous production was further confirmed by targeted knock-out experiments involving key regulators of the biosynthetic pathways. We were further able to revise the core structure of maduralactomycin A, using a computational approach
Heterologous expression of the cryptic mdk gene cluster and structural revision of maduralactomycin A
No full textAfter conducting an in silico analysis of the cryptic mdk cluster region and performing transcriptomic studies, an integrative Streptomyces BAC Vector containing the mdk gene sequence was constructed. The heterologous expression of the mdk cluster in Streptomyces albus J1074 resulted in the production of the angucyclic product, seongomycin, which allowed for the assesment of its antibacterial, antiproliferative, and antiviral activities. Heterologous production was further confirmed by targeted knock-out experiments involving key regulators of the biosynthetic pathways. We were further able to revise the core structure of maduralactomycin A, using a computational approach
Gene cluster activation in a bacterial symbiont leads to halogenated angucyclic maduralactomycins and spirocyclic actinospirols
No full textPlease read abstract in the article.https://pubs.acs.org/page/orlef7/about.htmlhj2021BiochemistryForestry and Agricultural Biotechnology Institute (FABI)GeneticsMicrobiology and Plant Patholog
