1,796 research outputs found
SCET sum rules for B->P and B->V transition form factors
We investigate sum rules for heavy-to-light transition form factors at large
recoil derived from correlation functions with interpolating currents for light
pseudoscalar or vector fields in soft-collinear effective theory (SCET). We
consider both, factorizable and non-factorizable contributions at leading power
in the Lambda/m_b expansion and to first order in the strong coupling constant
alpha_s, neglecting contributions from 3-particle distribution amplitudes in
the B-meson. We pay particular attention to various sources of parametric and
systematic uncertainties. We also discuss certain form factor ratios where part
of the hadronic uncertainties related to the B-meson distribution amplitude and
to logarithmically enhanced alpha_s corrections cancel.Comment: 27 pages, 19 figures, minor corrections, matches journal versio
Zebrafish embryos allow prediction of nanoparticle circulation times in mice and facilitate quantification of nanoparticle-cell interactions
The zebrafish embryo is a vertebrate well suited for visualizing nanoparticles at high resolution in live animals. Its optical transparency and genetic versatility allow noninvasive, real-time observations of vascular flow of nanoparticles and their interactions with cells throughout the body. As a consequence, this system enables the acquisition of quantitative data that are difficult to obtain in rodents. Until now, a few studies using the zebrafish model have only described semiquantitative results on key nanoparticle parameters. Here, a MACRO dedicated to automated quantitative methods is described for analyzing important parameters of nanoparticle behavior, such as circulation time and interactions with key target cells, macrophages, and endothelial cells. Direct comparison of four nanoparticle (NP) formulations in zebrafish embryos and mice reveals that data obtained in zebrafish can be used to predict NPs' behavior in the mouse model. NPs having long or short blood circulation in rodents behave similarly in the zebrafish embryo, with low circulation times being a consequence of NP uptake into macrophages or endothelial cells. It is proposed that the zebrafish embryo has the potential to become an important intermediate screening system for nanoparticle research to bridge the gap between cell culture studies and preclinical rodent models such as the mouse
Development and evaluation of a computer-based medical work assessment programme
Background: There are several ways to conduct a job task analysis in medical work environments including pencil-paper observations, interviews and questionnaires. However these methods implicate bias problems such as high inter-individual deviations and risks of misjudgement. Computer-based observation helps to reduce these problems. The aim of this paper is to give an overview of the development process of a computer-based job task analysis instrument for real-time observations to quantify the job tasks performed by physicians working in different medical settings. In addition reliability and validity data of this instrument will be demonstrated.
Methods: This instrument was developed in consequential steps. First, lists comprising tasks performed by physicians in different care settings were classified. Afterwards content validity of task lists was proved. After establishing the final task categories, computer software was programmed and implemented in a mobile personal computer. At least inter-observer reliability was evaluated. Two trained observers recorded simultaneously tasks of the same physician.
Results: Content validity of the task lists was confirmed by observations and experienced specialists of each medical area. Development process of the job task analysis instrument was completed successfully. Simultaneous records showed adequate interrater reliability.
Conclusion: Initial results of this analysis supported the validity and reliability of this developed method for assessing physicians' working routines as well as organizational context factors. Based on results using this method, possible improvements for health professionals' work organisation can be identified
Intracellularly Released Cholesterol from Polymer-Based Delivery Systems Alters Cellular Responses to Pneumolysin and Promotes Cell Survival
Cholesterol is highly abundant within all human body cells and modulates critical cellular functions related to cellular plasticity, metabolism, and survival. The cholesterol-binding toxin pneumolysin represents an essential virulence factor of Streptococcus pneumoniae in establishing pneumonia and other pneumococcal infections. Thus, cholesterol scavenging of pneumolysin is a promising strategy to reduce S. pneumoniae induced lung damage. There may also be a second cholesterol-dependent mechanism whereby pneumococcal infection and the presence of pneumolysin increase hepatic sterol biosynthesis. Here we investigated a library of polymer particles varying in size and composition that allow for the cellular delivery of cholesterol and their effects on cell survival mechanisms following pneumolysin exposure. Intracellular delivery of cholesterol by nanocarriers composed of Eudragit E100-PLGA rescued pneumolysin-induced alterations of lipid homeostasis and enhanced cell survival irrespective of neutralization of pneumolysin
QRS complex and T wave planarity for the efficacy prediction of automatic implantable defibrillators.
OBJECTIVE
To test the hypothesis that in recipients of primary prophylactic implantable cardioverter-defibrillators (ICDs), the non-planarity of ECG vector loops predicts (a) deaths despite ICD protection and (b) appropriate ICD shocks.
METHODS
Digital pre-implant ECGs were collected in 1948 ICD recipients: 21.4% females, median age 65 years, 61.5% ischaemic heart disease (IHD). QRS and T wave three-dimensional loops were constructed using singular value decomposition that allowed to measure the vector loop planarity. The non-planarity, that is, the twist of the three-dimensional loops out of a single plane, was related to all-cause mortality (n=294; 15.3% females; 68.7% IHD) and appropriate ICD shocks (n=162; 10.5% females; 87.7% IHD) during 5-year follow-up after device implantation. Using multivariable Cox regression, the predictive power of QRS and T wave non-planarity was compared with that of age, heart rate, left ventricular ejection fraction, QRS duration, spatial QRS-T angle, QTc interval and T-peak to T-end interval.
RESULTS
QRS non-planarity was significantly (p<0.001) associated with follow-up deaths despite ICD protection with HR of 1.339 (95% CI 1.165 to 1.540) but was only univariably associated with appropriate ICD shocks. Non-planarity of the T wave loop was the only ECG-derived index significantly (p<0.001) associated with appropriate ICD shocks with multivariable Cox regression HR of 1.364 (1.180 to 1.576) but was not associated with follow-up mortality.
CONCLUSIONS
The analysed data suggest that QRS and T wave non-planarity might offer distinction between patients who are at greater risk of death despite ICD protection and those who are likely to use the defibrillator protection
Genomics of Divergence along a Continuum of Parapatric Population Differentiation
MM received funding from the Max Planck innovation funds for this project. PGDF was supported by a Marie Curie European Reintegration Grant (proposal nr 270891). CE was supported by German Science Foundation grants (DFG, EI 841/4-1 and EI 841/6-1)
Observational and Dynamical Characterization of Main-Belt Comet P/2010 R2 (La Sagra)
We present observations of comet-like main-belt object P/2010 R2 (La Sagra)
obtained by Pan-STARRS 1 and the Faulkes Telescope-North on Haleakala in
Hawaii, the University of Hawaii 2.2 m, Gemini-North, and Keck I telescopes on
Mauna Kea, the Danish 1.54 m telescope at La Silla, and the Isaac Newton
Telescope on La Palma. An antisolar dust tail is observed from August 2010
through February 2011, while a dust trail aligned with the object's orbit plane
is also observed from December 2010 through August 2011. Assuming typical phase
darkening behavior, P/La Sagra is seen to increase in brightness by >1 mag
between August 2010 and December 2010, suggesting that dust production is
ongoing over this period. These results strongly suggest that the observed
activity is cometary in nature (i.e., driven by the sublimation of volatile
material), and that P/La Sagra is therefore the most recent main-belt comet to
be discovered. We find an approximate absolute magnitude for the nucleus of
H_R=17.9+/-0.2 mag, corresponding to a nucleus radius of ~0.7 km, assuming an
albedo of p=0.05. Using optical spectroscopy, we find no evidence of
sublimation products (i.e., gas emission), finding an upper limit CN production
rate of Q_CN<6x10^23 mol/s, from which we infer an H2O production rate of
Q_H2O<10^26 mol/s. Numerical simulations indicate that P/La Sagra is
dynamically stable for >100 Myr, suggesting that it is likely native to its
current location and that its composition is likely representative of other
objects in the same region of the main belt, though the relatively close
proximity of the 13:6 mean-motion resonance with Jupiter and the (3,-2,-1)
three-body mean-motion resonance with Jupiter and Saturn mean that dynamical
instability on larger timescales cannot be ruled out.Comment: 23 pages, 13 figures, accepted for publication in A
QRS micro-fragmentation as a mortality predictor.
AIMS
Fragmented QRS complex with visible notching on standard 12-lead electrocardiogram (ECG) is understood to represent depolarization abnormalities and to signify risk of cardiac events. Depolarization abnormalities with similar prognostic implications likely exist beyond visual recognition but no technology is presently suitable for quantification of such invisible ECG abnormalities. We present such a technology.
METHODS AND RESULTS
A signal processing method projects all ECG leads of the QRS complex into optimized three perpendicular dimensions, reconstructs the ECG back from this three-dimensional projection, and quantifies the difference (QRS 'micro'-fragmentation, QRS-μf) between the original and reconstructed signals. QRS 'micro'-fragmentation was assessed in three different populations: cardiac patients with automatic implantable cardioverter-defibrillators, cardiac patients with severe abnormalities, and general public. The predictive value of QRS-μf for mortality was investigated both univariably and in multivariable comparisons with other risk factors including visible QRS 'macro'-fragmentation, QRS-Mf. The analysis was made in a total of 7779 subjects of whom 504 have not survived the first 5 years of follow-up. In all three populations, QRS-μf was strongly predictive of survival (P < 0.001 univariably, and P < 0.001 to P = 0.024 in multivariable regression analyses). A similar strong association with outcome was found when dichotomizing QRS-μf prospectively at 3.5%. When QRS-μf was used in multivariable analyses, QRS-Mf and QRS duration lost their predictive value.
CONCLUSION
In three populations with different clinical characteristics, QRS-μf was a powerful mortality risk factor independent of several previously established risk indices. Electrophysiologic abnormalities that contribute to increased QRS-μf values are likely responsible for the predictive power of visible QRS-Mf.
KEY QUESTION
KEY FINDING
TAKE-HOME MESSAGE
QRS-μf is a strong predictor of worsened survival. It can be assessed in standard short-term 12-lead electrocardiograms
Extensive Copy-Number Variation of Young Genes across Stickleback Populations
MM received funding from the Max Planck innovation funds for this project. PGDF was supported by a Marie Curie European Reintegration Grant (proposal nr 270891). CE was supported by German Science Foundation grants (DFG, EI 841/4-1 and EI 841/6-1). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
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