131 research outputs found

    Hierarchical Traffic Management of Multi-AGV Systems With Deadlock Prevention Applied to Industrial Environments

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    This paper concerns the coordination and the traffic management of a group of Automated Guided Vehicles (AGVs) moving in a real industrial scenario, such as an automated factory or warehouse. The proposed methodology is based on a three-layer control architecture, which is described as follows: 1) the Top Layer (or Topological Layer) allows to model the traffic of vehicles among the different areas of the environment; 2) the Middle Layer allows the path planner to compute a traffic sensitive path for each vehicle; 3) the Bottom Layer (or Roadmap Layer) defines the final routes to be followed by each vehicle and coordinates the AGVs over time. In the paper we describe the coordination strategy we propose, which is executed once the routes are computed and has the aim to prevent congestions, collisions and deadlocks. The coordination algorithm exploits a novel deadlock prevention approach based on time-expanded graphs. Moreover, the presented control architecture aims at grounding theoretical methods to an industrial application by facing the typical practical issues such as graphs difficulties (load/unload locations, weak connections,), a predefined roadmap (constrained by the plant layout), vehicles errors, dynamical obstacles, etc. In this paper we propose a flexible and robust methodology for multi-AGVs traffic-aware management. Moreover, we propose a coordination algorithm, which does not rely on ad hoc assumptions or rules, to prevent collisions and deadlocks and to deal with delays or vehicle motion errors. Note to Practitioners-This paper concerns the coordination and the traffic management of a group of Automated Guided Vehicles (AGVs) moving in a real industrial scenario, such as an automated factory or warehouse. The proposed methodology is based on a three-layer control architecture, which is described as follows: 1) the Top Layer (or Topological Layer) allows to model the traffic of vehicles among the different areas of the environment; 2) the Middle Layer allows the path planner to compute a traffic sensitive path for each vehicle; 3) the Bottom Layer (or Roadmap Layer) defines the final routes to be followed by each vehicle and coordinates the AGVs over time. In the paper we describe the coordination strategy we propose, which is executed once the routes are computed and has the aim to prevent congestions, collisions and deadlocks. The coordination algorithm exploits a novel deadlock prevention approach based on time-expanded graphs. Moreover, the presented control architecture aims at grounding theoretical methods to an industrial application by facing the typical practical issues such as graphs difficulties (load/unload locations, weak connections, ), a predefined roadmap (constrained by the plant layout), vehicles errors, dynamical obstacles, etc. In this paper we propose a flexible and robust methodology for multi-AGVs traffic-aware management. Moreover, we propose a coordination algorithm, which does not rely on ad hoc assumptions or rules, to prevent collisions and deadlocks and to deal with delays or vehicle motion errors

    In the eye of the storm : the Italian economy and the eurozone crisis

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    The eurozone crisis had a more significant and longer-lasting impact on Italy than on virtually any other member state, with the effects still visible a decade after. The extent of the shock was surprising in view of progress Italy had apparently made in the 1990s in terms of enhancing its capacity to meet the demands of European Monetary Union. The explanation for this traumatic economic experience lies in Italy’s deep, long-term, structural tensions which were placed under severe pressure during the 1990s and which were cracked open by the 2011 sovereign debt crisis. These have had long-standing economic effects as well as political ramifications in terms of a significant change in the Italy–EU relationship

    Making wine safer: the case of ochratoxin A

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    This study aims to assess the risk of ochratoxin A (OTA) in European wine with the objective of reducing toxin levels through an integrated management of production and processing. All European countries of the Mediterranean basin are involved. Preliminary results indicate that OTA producing fungi are already present on grapes in the vineyard, prior to harvest. Vineyard location has more influence on OTA levels than grape variety. Weather patterns also seem to influence OTA levels. Results obtained from applications of various adjuvants aimed at reducing and/or eliminating OTA in wine are discussed.info:eu-repo/semantics/publishedVersio

    Diverse tick-borne microorganisms identified in free-living ungulates in Slovakia

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    Background: Free-living ungulates are hosts of ixodid ticks and reservoirs of tick-borne microorganisms in central Europe and many regions around the world. Tissue samples and engorged ticks were obtained from roe deer, red deer, fallow deer, mouflon, and wild boar hunted in deciduous forests of south-western Slovakia. DNA isolated from these samples was screened for the presence of tick-borne microorganisms by PCR-based methods. Results: Ticks were found to infest all examined ungulate species. The principal infesting tick was Ixodes ricinus, identified on 90.4% of wildlife, and included all developmental stages. Larvae and nymphs of Haemaphysalis concinna were feeding on 9.6% of wildlife. Two specimens of Dermacentor reticulatus were also identified. Ungulates were positive for A. phagocytophilum and Theileria spp. Anaplasma phagocytophilum was found to infect 96.1% of cervids, 88.9% of mouflon, and 28.2% of wild boar, whereas Theileria spp. was detected only in cervids (94.6%). Importantly, a high rate of cervids (89%) showed mixed infections with both these microorganisms. In addition to A. phagocytophilum and Theileria spp., Rickettsia helvetica, R. monacensis, unidentified Rickettsia sp., Coxiella burnetii, "Candidatus Neoehrlichia mikurensis", Borrelia burgdorferi (s.l.) and Babesia venatorum were identified in engorged I. ricinus. Furthermore, A. phagocytophilum, Babesia spp. and Theileria spp. were detected in engorged H. concinna. Analysis of 16S rRNA and groEL gene sequences revealed the presence of five and two A. phagocytophilum variants, respectively, among which sequences identified in wild boar showed identity to the sequence of the causative agent of human granulocytic anaplasmosis (HGA). Phylogenetic analysis of Theileria 18S rRNA gene sequences amplified from cervids and engorged I. ricinus ticks segregated jointly with sequences of T. capreoli isolates into a moderately supported monophyletic clade. Conclusions: The findings indicate that free-living ungulates are reservoirs for A. phagocytophilum and Theileria spp. and engorged ixodid ticks attached to ungulates are good sentinels for the presence of agents of public and veterinary concern. Further analyses of the A. phagocytophilum genetic variants and Theileria species and their associations with vector ticks and free-living ungulates are required.Fil: Kazimírová, Mária. Slovak Academy of Sciences. Institute of Zoology; EslovaquiaFil: Hamšíková, Zuzana. Slovak Academy of Sciences. Institute of Zoology; EslovaquiaFil: Spitalská, Eva. Slovak Academy of Sciences. Institute of Virology. Biomedical Research Center,; EslovaquiaFil: Minichová, Lenka. Slovak Academy of Sciences. Institute of Virology. Biomedical Research Center,; EslovaquiaFil: Mahríková, Lenka. Slovak Academy of Sciences. Institute of Zoology; EslovaquiaFil: Caban, Radoslav. Široká ; EslovaquiaFil: Sprong, Hein. National Institute for Public Health and Environment.Laboratory for Zoonoses and Environmental Microbiology; Países BajosFil: Fonville, Manoj. National Institute for Public Health and Environment.Laboratory for Zoonoses and Environmental Microbiology; Países BajosFil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Kocianová, Elena. Slovak Academy of Sciences. Institute of Virology. Biomedical Research Center,; Eslovaqui

    Lateral flow immunoassay for on-site detection of Xanthomonas arboricola pv. Pruni in symptomatic field samples

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    [EN] Xanthomonas arboricola pv. pruni is a quarantine pathogen and the causal agent of the bacterial spot disease of stone fruits and almond, a major threat to Prunus species. Rapid and specific detection methods are essential to improve disease management, and therefore a prototype of a lateral flow immunoassay (LFIA) was designed for the detection of X. arboricola pv. pruni in symptomatic field samples. It was developed by producing polyclonal antibodies which were then combined with carbon nanoparticles and assembled on nitrocellulose strips. The specificity of the LFIA was tested against 87 X. arboricola pv. pruni strains from different countries worldwide, 47 strains of other Xanthomonas species and 14 strains representing other bacterial genera. All X. arboricola pv. pruni strains were detected and cross-reactions were observed only with four strains of X. arboricola pv. corylina, a hazelnut pathogen that does not share habitat with X. arboricola pv. pruni. The sensitivity of the LFIA was assessed with suspensions from pure cultures of three X. arboricola pv. pruni strains and with spiked leaf extracts prepared from four hosts inoculated with this pathogen (almond, apricot, Japanese plum and peach). The limit of detection observed with both pure cultures and spiked samples was 10(4) CFU ml(-1). To demonstrate the accuracy of the test, 205 samples naturally infected with X. arboricola pv. pruni and 113 samples collected from healthy plants of several different Prunus species were analyzed with the LFIA. Results were compared with those obtained by plate isolation and real time PCR and a high correlation was found among techniques. Therefore, we propose this LFIA as a screening tool that allows a rapid and reliable diagnosis of X. arboricola pv. pruni in symptomatic plants.The work was supported by the following: Instituto Nacional de Tecnologia Agraria y Alimentaria, Project RTA-2011-00140-C03-01 (http://www.inia.es), PLS MTG EMN MML; Instituto Nacional de Tecnologia Agraria y Alimentaria, FPI-INIA grant (http://www.inia.es), PLS; Generalitat Valenciana (Prometeo II 2014/040) (http://www.gva.es), PN RP AM; Ministerio de Economia y Competitividad (MINECO) (CTQ2013-45875R) (http://www.mineco.gob.es), PN RP AM; European Social Fund, PLS MTG EMN MML; and European Regional Development Fund, PLS MTG EMN MML.López-Soriano, P.; Noguera Murray, PS.; Gorris, MT.; Puchades, R.; Maquieira Catala, A.; Marco-Noales, E.; López, M. (2017). Lateral flow immunoassay for on-site detection of Xanthomonas arboricola pv. Pruni in symptomatic field samples. PLoS ONE. 12(4):1-13. https://doi.org/10.1371/journal.pone.0176201S113124Tjou-Tam-Sin, N. N. A., van de Bilt, J. L. J., Bergsma-Vlami, M., Koenraadt, H., Naktuinbouw, J. W., van Doorn, J., … Martin, W. S. (2012). First Report of Xanthomonas arboricola pv. pruni in Ornamental Prunus laurocerasus in the Netherlands. Plant Disease, 96(5), 759-759. doi:10.1094/pdis-04-11-0265-pdnPothier, J. F., Vorhölter, F.-J., Blom, J., Goesmann, A., Pühler, A., Smits, T. H. M., & Duffy, B. (2011). The ubiquitous plasmid pXap41 in the invasive phytopathogen Xanthomonas arboricola pv. pruni: complete sequence and comparative genomic analysis. FEMS Microbiology Letters, 323(1), 52-60. doi:10.1111/j.1574-6968.2011.02352.xPalacio-Bielsa, A., Cubero, J., Cambra, M. A., Collados, R., Berruete, I. M., & López, M. M. (2010). Development of an Efficient Real-Time Quantitative PCR Protocol for Detection ofXanthomonas arboricolapv. pruni inPrunusSpecies. Applied and Environmental Microbiology, 77(1), 89-97. doi:10.1128/aem.01593-10Xanthomonas arboricola pv. pruni. (2006). EPPO Bulletin, 36(1), 129-133. doi:10.1111/j.1365-2338.2006.00925.xPagani MC. An ABC transporter protein and molecular diagnoses of Xanthomonas arboricola pv. pruni causing bacterial spot of stone fruits. Raleigh, North Carolina, USA: North Carolina State University, PhD thesis. 2004; Online, http://repository.lib.ncsu.edu/ir/bitstream/1840.16/4540/1/etd.pdfPark, S. Y., Lee, Y. S., Koh, Y. J., Hur, J.-S., & Jung, J. S. (2010). Detection of Xanthomonas arboricola pv. pruni by PCR using primers based on DNA sequences related to the hrp genes. The Journal of Microbiology, 48(5), 554-558. doi:10.1007/s12275-010-0072-3Pothier, J. F., Pagani, M. C., Pelludat, C., Ritchie, D. F., & Duffy, B. (2011). A duplex-PCR method for species- and pathovar-level identification and detection of the quarantine plant pathogen Xanthomonas arboricola pv. pruni. Journal of Microbiological Methods, 86(1), 16-24. doi:10.1016/j.mimet.2011.03.019Ballard, E. L., Dietzgen, R. G., Sly, L. I., Gouk, C., Horlock, C., & Fegan, M. (2011). Development of a Bio-PCR Protocol for the Detection of Xanthomonas arboricola pv. pruni. Plant Disease, 95(9), 1109-1115. doi:10.1094/pdis-09-10-0650Boonham, N., Glover, R., Tomlinson, J., & Mumford, R. (2008). Exploiting generic platform technologies for the detection and identification of plant pathogens. European Journal of Plant Pathology, 121(3), 355-363. doi:10.1007/s10658-008-9284-3Posthuma-Trumpie, G. A., Korf, J., & van Amerongen, A. (2008). Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey. Analytical and Bioanalytical Chemistry, 393(2), 569-582. doi:10.1007/s00216-008-2287-2De Boer, S. H., & López, M. M. (2012). New Grower-Friendly Methods for Plant Pathogen Monitoring. Annual Review of Phytopathology, 50(1), 197-218. doi:10.1146/annurev-phyto-081211-172942Thornton, C. R., Groenhof, A. C., Forrest, R., & Lamotte, R. (2004). A One-Step, Immunochromatographic Lateral Flow Device Specific to Rhizoctonia solani and Certain Related Species, and Its Use to Detect and Quantify R. solani in Soil. Phytopathology®, 94(3), 280-288. doi:10.1094/phyto.2004.94.3.280Lane, C. R., Hobden, E., Walker, L., Barton, V. C., Inman, A. J., Hughes, K. J. D., … Barker, I. (2007). Evaluation of a rapid diagnostic field test kit for identification of Phytophthora species, including P. ramorum and P. kernoviae at the point of inspection. Plant Pathology, 56(5), 828-835. doi:10.1111/j.1365-3059.2007.01615.xSafenkova, I., Zherdev, A., & Dzantiev, B. (2012). Factors influencing the detection limit of the lateral-flow sandwich immunoassay: a case study with potato virus X. Analytical and Bioanalytical Chemistry, 403(6), 1595-1605. doi:10.1007/s00216-012-5985-8Safenkova, I. V., Pankratova, G. K., Zaitsev, I. A., Varitsev, Y. A., Vengerov, Y. Y., Zherdev, A. V., & Dzantiev, B. B. (2016). Multiarray on a test strip (MATS): rapid multiplex immunodetection of priority potato pathogens. Analytical and Bioanalytical Chemistry, 408(22), 6009-6017. doi:10.1007/s00216-016-9463-6Braun-Kiewnick, A., Altenbach, D., Oberhänsli, T., Bitterlin, W., & Duffy, B. (2011). A rapid lateral-flow immunoassay for phytosanitary detection of Erwinia amylovora and on-site fire blight diagnosis. Journal of Microbiological Methods, 87(1), 1-9. doi:10.1016/j.mimet.2011.06.015Safenkova, I. V., Zaitsev, I. A., Pankratova, G. K., Varitsev, Y. A., Zherdev, A. V., & Dzantiev, B. B. (2014). Lateral flow immunoassay for rapid detection of potato ring rot caused by Clavibacter michiganensis subsp. sepedonicus. Applied Biochemistry and Microbiology, 50(6), 675-682. doi:10.1134/s0003683814120011Hodgetts, J., Karamura, G., Johnson, G., Hall, J., Perkins, K., Beed, F., … Smith, J. (2014). Development of a lateral flow device for in-field detection and evaluation of PCR-based diagnostic methods forXanthomonas campestrispv.musacearum, the causal agent of banana xanthomonas wilt. Plant Pathology, 64(3), 559-567. doi:10.1111/ppa.12289Noguera, P., Posthuma-Trumpie, G. A., van Tuil, M., van der Wal, F. J., de Boer, A., Moers, A. P. H. A., & van Amerongen, A. (2010). Carbon nanoparticles in lateral flow methods to detect genes encoding virulence factors of Shiga toxin-producing Escherichia coli. Analytical and Bioanalytical Chemistry, 399(2), 831-838. doi:10.1007/s00216-010-4334-zCambra M, López MM. Titration of Agrobacterium radiobacter var. tumefaciens antibodies by using enzyme labeled anti-rabbit γ-globulines (ELISA indirect method). In: Proceedings of the 4th International Conference on Plant Pathogenic Bacteria, ed. Station Pathologie Végétale, INRA Angers, 1978. pp: 327–331.O’Keeffe, M., Crabbe, P., Salden, M., Wichers, J., Van Peteghem, C., Kohen, F., … Moneti, G. (2003). Preliminary evaluation of a lateral flow immunoassay device for screening urine samples for the presence of sulphamethazine. Journal of Immunological Methods, 278(1-2), 117-126. doi:10.1016/s0022-1759(03)00207-2PM 7/98 (2) Specific requirements for laboratories preparing accreditation for a plant pest diagnostic activity. (2014). EPPO Bulletin, 44(2), 117-147. doi:10.1111/epp.12118Lamichhane, J. R., & Varvaro, L. (2013). Xanthomonas arboricoladisease of hazelnut: current status and future perspectives for its management. Plant Pathology, 63(2), 243-254. doi:10.1111/ppa.12152Fischer-Le Saux, M., Bonneau, S., Essakhi, S., Manceau, C., & Jacques, M.-A. (2015). Aggressive Emerging Pathovars of Xanthomonas arboricola Represent Widespread Epidemic Clones Distinct from Poorly Pathogenic Strains, as Revealed by Multilocus Sequence Typing. Applied and Environmental Microbiology, 81(14), 4651-4668. doi:10.1128/aem.00050-15Bühlmann, A., Pothier, J. F., Tomlinson, J. A., Frey, J. E., Boonham, N., Smits, T. H. M., & Duffy, B. (2012). Genomics-informed design of loop-mediated isothermal amplification for detection of phytopathogenicXanthomonas arboricolapv. pruniat the intraspecific level. Plant Pathology, 62(2), 475-484. doi:10.1111/j.1365-3059.2012.02654.xGarita-Cambronero, J., Palacio-Bielsa, A., López, M. M., & Cubero, J. (2017). Pan-Genomic Analysis Permits Differentiation of Virulent and Non-virulent Strains of Xanthomonas arboricola That Cohabit Prunus spp. and Elucidate Bacterial Virulence Factors. Frontiers in Microbiology, 8. doi:10.3389/fmicb.2017.00573Ghezzi, J. I., & Steck, T. R. (1999). Induction of the viable but non-culturable condition in Xanthomonas campestris pv. campestris in liquid microcosms and sterile soil. FEMS Microbiology Ecology, 30(3), 203-208. doi:10.1111/j.1574-6941.1999.tb00648.xDel Campo, R., Russi, P., Mara, P., Mara, Hã©., Peyrou, M., de León, I. P., & Gaggero, C. (2009). Xanthomonas axonopodispv.citriâ enters the VBNC state after copper treatment and retains its virulence. FEMS Microbiology Letters, 298(2), 143-148. doi:10.1111/j.1574-6968.2009.01709.
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