Marktforschung in der Praxis: Kundenpräferenzen beim Kauf eines neuen Fernsehgerätes

In der heutigen Zeit sind Medien aller Art im alltäglichen Leben anzutreffen. Ob früh am Morgen die frisch gedruckte Zeitung, das Radio in der Küche, der Lieblingsfilm im Fernsehen oder das Internet via Mobiltelefon. Viele Menschen können sich heutzutage ein Leben ohne Mobiltelefon oder Fernsehgerät nicht mehr vorstellen. Die Industrie hat dies erkannt und bringt fast täglich neue Weiterentwicklungen auf den Markt. Smartphones, die beinahe so viel können wie ein Laptop oder Fernseher, die den Zuschauer via 3D-Technologie in das Geschehen eintauchen lassen, entwickeln sich zu Standardprodukten. Bei der heutigen Geschwindigkeit der Neuentwicklungen und den immer kürzer werdenden Produktlebenszyklen ist es besonders wichtig, den Endverbraucher vom Mehrwert zu überzeugen. In diesem Zusammenhang ist es notwendig herauszukristallisieren, worauf der Endkunde Wert legt und welche Informationsschwächen er hat. Hierzu haben acht Studierende der TH Wildau aus dem Fachbereich Betriebswirtschaft/Wirtschaftsinformatik eine empirische Studie entwickelt.In your everyday life, media of all kinds are found. Whether early in the morning, freshly printed newspaper, the radio in the kitchen, favorite movies on TV or the Internet via mobile phone. Many people can not imagine a life without mobile phone or TV. The industry has recognized that and almost every day they have new developments for the market. Smartphones have nearly as much as functions as a laptop or a television and are able to bring the viewers into actions via 3D technology as a standard. Because of the speed of new developments and the shortening product life cycle it is especially important to convince the consumer«s added value. In this case it is important to have a look what is significant for the end consumer and what lack of information does the consumer have. Concerning these problems, eight students of the University of Applied Science Wildau from the Department of Business Administration and Business Informatics designed an empirical study

Caribbean Corals in Crisis: Record Thermal Stress, Bleaching, and Mortality in 2005

BACKGROUND The rising temperature of the world's oceans has become a major threat to coral reefs globally as the severity and frequency of mass coral bleaching and mortality events increase. In 2005, high ocean temperatures in the tropical Atlantic and Caribbean resulted in the most severe bleaching event ever recorded in the basin. METHODOLOGY/PRINCIPAL FINDINGS Satellite-based tools provided warnings for coral reef managers and scientists, guiding both the timing and location of researchers' field observations as anomalously warm conditions developed and spread across the greater Caribbean region from June to October 2005. Field surveys of bleaching and mortality exceeded prior efforts in detail and extent, and provided a new standard for documenting the effects of bleaching and for testing nowcast and forecast products. Collaborators from 22 countries undertook the most comprehensive documentation of basin-scale bleaching to date and found that over 80% of corals bleached and over 40% died at many sites. The most severe bleaching coincided with waters nearest a western Atlantic warm pool that was centered off the northern end of the Lesser Antilles. CONCLUSIONS/SIGNIFICANCE Thermal stress during the 2005 event exceeded any observed from the Caribbean in the prior 20 years, and regionally-averaged temperatures were the warmest in over 150 years. Comparison of satellite data against field surveys demonstrated a significant predictive relationship between accumulated heat stress (measured using NOAA Coral Reef Watch's Degree Heating Weeks) and bleaching intensity. This severe, widespread bleaching and mortality will undoubtedly have long-term consequences for reef ecosystems and suggests a troubled future for tropical marine ecosystems under a warming climate.This work was partially supported by salaries from the NOAA Coral Reef Conservation Program to the NOAA Coral Reef Conservation Program authors. NOAA provided funding to Caribbean ReefCheck investigators to undertake surveys of bleaching and mortality. Otherwise, no funding from outside authors' institutions was necessary for the undertaking of this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

The missing link: Bordetella petrii is endowed with both the metabolic versatility of environmental bacteria and virulence traits of pathogenic Bordetellae

Gross R, Guzman CA, Sebaihia M, et al. The missing link: Bordetella petrii is endowed with both the metabolic versatility of environmental bacteria and virulence traits of pathogenic Bordetellae. BMC Genomics. 2008;9(1): 449.Background: Bordetella petrii is the only environmental species hitherto found among the otherwise host-restricted and pathogenic members of the genus Bordetella. Phylogenetically, it connects the pathogenic Bordetellae and environmental bacteria of the genera Achromobacter and Alcaligenes, which are opportunistic pathogens. B. petrii strains have been isolated from very different environmental niches, including river sediment, polluted soil, marine sponges and a grass root. Recently, clinical isolates associated with bone degenerative disease or cystic fibrosis have also been described. Results: In this manuscript we present the results of the analysis of the completely annotated genome sequence of the B. petrii strain DSMZ12804. B. petrii has a mosaic genome of 5,287,950 bp harboring numerous mobile genetic elements, including seven large genomic islands. Four of them are highly related to the clc element of Pseudomonas knackmussii B13, which encodes genes involved in the degradation of aromatics. Though being an environmental isolate, the sequenced B. petrii strain also encodes proteins related to virulence factors of the pathogenic Bordetellae, including the filamentous hemagglutinin, which is a major colonization factor of B. pertussis, and the master virulence regulator BvgAS. However, it lacks all known toxins of the pathogenic Bordetellae. Conclusion: The genomic analysis suggests that B. petrii represents an evolutionary link between free-living environmental bacteria and the host-restricted obligate pathogenic Bordetellae. Its remarkable metabolic versatility may enable B. petrii to thrive in very different ecological niches

Correction: Protocol of the Healthy Brain Study:An accessible resource for understanding the human brain and how it dynamically and individually operates in its bio-social context

[This corrects the article DOI: 10.1371/journal.pone.0260952.]

Automatisierung von Black Box Softwaretests mit risikobasierter Priorisierung bei Eurogate IT Services GmbH

Thema dieser Arbeit ist die Vorgehensweise zur Einführung von automatisierten Black Box Softwaretests in einem betrieblichen Umfeld. Anhand von zwei unterschiedlichen Projekten werden Voraussetzungen und wichtige Schritte zur Implementierungbeschrieben. Dies umfasst die Analyse der zu testenden Programme und Testprozesse, die Auswahl von Automatisierungstools sowie die konkrete Arbeit mit diesen Tools. Die Arbeit mit den Automatisierungstool HP QuickTest Professional wird mit Praxisbeispielen verdeutlicht.This thesis specifies an approach to the implementation of automatic black-box software tests in a business environment. Requirements and essential steps of an implementation are described on the basis of two different projects. This concept includes the analysis of the applications under test and the test process, the selection of automatic test tools as well as the practical work with these tools. The work with the automation tool HP QuickTest Professional is exemplified by practical examples

RV Poseidon Cruise P413 - Ligurian Sea/Gulf of Genoa - Cruise Report

10.05. - 28.05.2011 Ligurische See, Golf von Genu

KDEL Receptor 1 Contributes to Cell Surface Association of Protein Disulfide Isomerases

Background/Aims: Endoplasmic reticulum (ER)-resident proteins with a C-terminal KDEL ER-retention sequence are captured in the Golgi apparatus by KDEL receptors (KDELRs). The binding of such proteins to these receptors induces their retrograde transport. Nevertheless, some KDEL proteins, such as Protein Disulfide Isomerases (PDIs), are found at the cell surface. PDIs target disulfide bridges in the extracellular domains of proteins, such as integrins or A Disintegrin And Metalloprotease 17 (ADAM17) leading to changes in the structure and function of these molecules. Integrins become activated and ADAM17 inactivated upon disulfide isomerization. The way that PDIs escape from retrograde transport and reach the plasma membrane remains far from clear. Various mechanisms might exist, depending on whether a local cell surface association or a more global secretion is required. Methods: To get a more detailed insight in the transport of PDIs to the cell surface, methods such as cell surface biotinylation, flow cytometric analysis, immunoprecipitation, fluorescence microscopy as well as labeling of cells with fluorescence labled recombinant PDIA6 was performed. Results: Here, we show that the C-terminal KDEL ER retention sequence is sufficient to prevent secretion of PDIA6 into the extracellular space but is mandatory for its association with the cell surface. The cell surface trafficking of PDIA1, PDIA3, and PDIA6 is dependent on KDELR1, which travels in a dynamic manner to the cell surface. This transport is assumed to result in PDI cell surface association, which differs from PDI inducible secretion into the extracellular space. Distinct PDIs differ in their trafficking properties. Endogenous KDELR1, detectable at the cell surface, might be involved not only in the transport of cell-surface-associated PDIs, but also in their retrieval and internalization from the extracellular space. Conclusion: Beside their ER retention motive PDIs travel to the cell surface. Here they target different proteins to render their function. To escape the ER PDIs travel via various pathways. One of them depends on the KDELR1, which can transport its target to the cell surface, where it is to be expected to release its cargo in close vicinity to its target molecules. Hence, the KDEL sequence is needed for cell surface association of PDIs, such as PDIA6