A simple compensation method for the accurate measurement of magnetic losses with a single strip tester

International audienceWe present a new method for the accurate characterization of soft magnetic sheets using a permeameter based on the precise compensation of the magnetomotive force (MMF) drop in the flux-closing yoke. It has been developed in order to overcome the systematic uncertainty affecting the value of the magnetic fieldstrength in single sheet testers when obtained, according to the standards, through the measurement of the magnetizing current. This phenomenon is more critical for high permeability materials, because of the reduced MMF drop across the sample. While additional sensors and auxiliary windings have been proposed in the literature, a novel approach is demonstrated here, based on the use of the permeameter upper half yoke as the MMF drop sensor and of an auxiliary winding on the lower half yoke, implementing compensation. This solution, dispensing one from dealing with the usually small signal levels of the conventional MMF drop sensors (e.g. Chattock coils), provides best results with the introduction of wedge-shaped magnetic poles, in order to accurately define the magnetic path length. The method is validated by measurements of power loss, apparent power, and hysteresis cycles on non-oriented and grain-oriented Fe-Si steel sheets, which are compared with local measurements performed on the same samples using H-coil and B-coil across a uniformly magnetized region

Hemodynamic and antifibrotic effects of a selective liver nitric oxide donor V-PYRRO/NO in bile duct ligated rats.

AIM: To assess whether a liver specific nitric oxide (NO) donor (V-PYRRO/NO) would prevent the development of portal hypertension and liver fibrosis in rats with bile duct ligation (BDL). METHODS: Treatment (placebo or V-PYRRO/NO 0.53 micromol/kg per hour) was administered i.v. to rats 2 d before BDL (D-2) and maintained until the day of hemodynamic measurement (D26). Intra-hepatic NO level was estimated by measuring liver cGMP level. Effects of V-PYRRO/NO on liver fibrosis and lipid peroxidation were also assessed. RESULTS: Compared to placebo treatment, V-PYRRO/NO improved splanchnic hemodynamics in BDL rats: portal pressure was significantly reduced by 27% (P<0.0001) and collateral circulation development was almost completely blocked (splenorenal shunt blood flow by 74%, P=0.007). Moreover, V-PYRRO/NO significantly prevented liver fibrosis development in BDL rats (by 30% in hepatic hydroxyproline content and 31% in the area of fibrosis, P<0.0001 respectively), this effect being probably due to a decrease in lipid peroxidation by 44% in the hepatic malondialdehyde level (P=0.007). Interestingly, we observed a significant and expected increase in liver cGMP, without any systemic hemodynamic effects (mean arterial pressure, vascular systemic resistance and cardiac output) in both sham-operated and BDL rats treated with V-PYRRO/NO. This result is in accordance with studies on V-PYRRO/NO metabolism showing a specific release of NO in the liver. CONCLUSION: Continuous administrations of V-PYRRO/NO in BDL rats improved liver fibrosis and splanchnic hemodynamics without any noxious systemic hemo-dynamic effects

Pelizaeus-Merzbacher-Like disease presentation of MCT8 mutated male subjects.

Pelizaeus-Merzbacher Disease is an X-linked hypomyelinatiing leukodystrophy. We report mutations in the thyroid hormone transporter gene MCT8 in 11% of 53 families affected by hypomyelinating leukodystrophies of unknown aetiology. The 12 MCT8 mutated patients express initially a Pelizaeus-Merzbacher-Like disease phenotype with a latter unusual improvement of magnetic resonance imaging white matter signal despite absence of clinical progression. This observation underlines the interest of determining both free T3 and free T4 serum concentrations to screen for MCT8 mutations in young patients (<3 y) with a severe Pelizaeus-Merzbacher-Like disease presentation or older severe mentally retarded male patients with "hypomyelinated" regions

Probing dust grain evolution in IM Lupi's circumstellar disc. Multi-wavelength observations and modelling of the dust disc

We present a panchromatic study, involving a multiple technique approach, of the circumstellar disc surrounding the T Tauri star IM Lupi (Sz 82). We have undertaken a comprehensive observational study of IM Lupi using photometry, spectroscopy, millimetre interferometry and multi-wavelength imaging. For the first time, the disc is resolved from optical and near-infrared wavelengths in scattered light, to the millimetre regime in thermal emission. Our data-set, in conjunction with existing photometric data, provides an extensive coverage of the spectral energy distribution, including a detailed spectrum of the silicate emission bands. We have performed a simultaneous modelling of the various observations, using the radiative transfer code MCFOST, and analysed a grid of models over a large fraction of the parameter space via Bayesian inference. We have constructed a model that can reproduce all of the observations of the disc. Our analysis illustrates the importance of combining a wide range of observations in order to fully constrain the disc model, with each observation providing a strong constraint only on some aspects of the disc structure and dust content. Quantitative evidence of dust evolution in the disc is obtained: grain growth up to millimetre-sized particles, vertical stratification of dust grains with micrometric grains close to the disc surface and larger grains which have settled towards the disc midplane, and possibly the formation of fluffy aggregates and/or ice mantles around grains.Comment: 20 pages, 13 figures. Accepted for publication in A&

Laser Doppler flowmetry signals: pointwise Hölder exponents of experimental signals from young healthy subjects and numerically simulated data

We analyze the complexity of laser Doppler flowmetry (LDF) signals which give a peripheral view of the cardiovascular system. For this purpose, experimental and numerically simulated LDF signals are processed. The experimental signals are recorded in young healthy subjects. The numerically simulated LDF data are computed from a model containing six nonlinear coupled oscillators reflecting six almost periodic rhythmic activities present in experimental LDF signals. In the model, the oscillators are coupled with both linear and parametric couplings in order to represent cardiovascular system behaviors. To our knowledge this modeling has never been proposed yet. The complexity of all the experimental and simulated signals is studied by the computation of pointwise Hölder exponents. The latter identify the possible multifractal characteristics of data. The pointwise Hölder exponents are determined with a parametric generalized quadratic variation based estimation method first calibrated from white noise measures. The results of our signal processing analysis show that experimental LDF signals are weakly multifractal for young healthy subjects at rest. Furthermore, our findings together with another recent work of our group show that pointwise Hölder exponents of the simulated data do not describe the ones of the young healthy subjects but are closer to the ones of elderly healthy people. This paper provides useful information to go deeper into the modeling of LDF data, that could bring enlightenment for a better understanding of the peripheral cardiovascular system

Horizontal DNA transfer mechanisms of bacteria as weapons of intragenomic conflict

Horizontal DNA transfer (HDT) is a pervasive mechanism of diversification in many microbial species, but its primary evolutionary role remains controversial. Much recent research has emphasised the adaptive benefit of acquiring novel DNA, but here we argue instead that intragenomic conflict provides a coherent framework for understanding the evolutionary origins of HDT. To test this hypothesis, we developed a mathematical model of a clonally descended bacterial population undergoing HDT through transmission of mobile genetic elements (MGEs) and genetic transformation. Including the known bias of transformation toward the acquisition of shorter alleles into the model suggested it could be an effective means of counteracting the spread of MGEs. Both constitutive and transient competence for transformation were found to provide an effective defence against parasitic MGEs; transient competence could also be effective at permitting the selective spread of MGEs conferring a benefit on their host bacterium. The coordination of transient competence with cell-cell killing, observed in multiple species, was found to result in synergistic blocking of MGE transmission through releasing genomic DNA for homologous recombination while simultaneously reducing horizontal MGE spread by lowering the local cell density. To evaluate the feasibility of the functions suggested by the modelling analysis, we analysed genomic data from longitudinal sampling of individuals carrying Streptococcus pneumoniae. This revealed the frequent within-host coexistence of clonally descended cells that differed in their MGE infection status, a necessary condition for the proposed mechanism to operate. Additionally, we found multiple examples of MGEs inhibiting transformation through integrative disruption of genes encoding the competence machinery across many species, providing evidence of an ongoing "arms race." Reduced rates of transformation have also been observed in cells infected by MGEs that reduce the concentration of extracellular DNA through secretion of DNases. Simulations predicted that either mechanism of limiting transformation would benefit individual MGEs, but also that this tactic's effectiveness was limited by competition with other MGEs coinfecting the same cell. A further observed behaviour we hypothesised to reduce elimination by transformation was MGE activation when cells become competent. Our model predicted that this response was effective at counteracting transformation independently of competing MGEs. Therefore, this framework is able to explain both common properties of MGEs, and the seemingly paradoxical bacterial behaviours of transformation and cell-cell killing within clonally related populations, as the consequences of intragenomic conflict between self-replicating chromosomes and parasitic MGEs. The antagonistic nature of the different mechanisms of HDT over short timescales means their contribution to bacterial evolution is likely to be substantially greater than previously appreciated