Ten‐Year Clinical Experience with the Lumenless, Catheter‐Delivered, 4.1‐Fr Diameter Pacing Lead in Patients with and without Congenital Heart

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/135972/1/pace12995.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/135972/2/pace12995_am.pd

Shaped stone balls were used for bone marrow extraction at Lower Paleolithic Qesem Cave, Israel

The presence of shaped stone balls at early Paleolithic sites has attracted scholarly attention since the pioneering work of the Leakeys in Olduvai, Tanzania. Despite the persistent presence of these items in the archaeological record over a period of two million years, their function is still debated. We present new results from Middle Pleistocene Qesem Cave on the use of these implements as percussion tools. Use-wear and abundant bone and fat residues found on ten shaped stone balls indicate crushing of fresh bones by thrusting percussion and provide direct evidence for the use of these items to access bone marrow of animal prey at this site. Two experiments conducted to investigate and verify functional aspects proved Qesem Cave shaped stone balls are efficient for bone processing and provide a comfortable grip and useful active areas for repeated use. Notably, the patina observed on the analyzed items precedes their use at the cave, indicating that they were collected by Qesem inhabitants, most probably from older Lower Paleolithic Acheulian sites. Thus, our results refer only to the final phases of the life of the items, and we cannot attest to their original function. As bone marrow played a central role in human nutrition in the Lower Paleolithic, and our experimental results show that the morphology and characteristics of shaped stone ball replicas are well-suited for the extraction of bone marrow, we suggest that these features might have been the reason for their collection and use at Qesem Cave. These results shed light on the function of shaped stone balls and are consistent with the significance of animal fat in the caloric intake of Middle Pleistocene humans as shown by the archeozoological evidence at Qesem Cave and possibly beyondWe acknowledge funding received for this project through the European Research Council (ERC Starting Grant Project HIDDEN FOODS, G.A. no. 639286 to EC). CL is grateful to MAECI (Italian Ministry for the Foreign Affairs) for its funding support to this project. EA is grateful to the Azrieli Foundation for the award of an Azrieli Fellowship. This study was funded by the grant UT 41/4-1 “Cultural and biological transformations in the Late Middle Pleistocene (420- 200 ka ago) at Qesem Cave, Israel: In search for a post-Homo erectus lineage in the Levantine corridor” (A. Gopher, R. Barkai, Th. Uthmeier) of the Deutsche Forschungsgemeinschaft (DFG). The Qesem Cave excavation project was previously supported by the Israel Science Foundation, the CARE Archaeological Foundation, the Leakey Foundation, the Wenner-Gren Foundation, the Dan David Foundation, and the German Research Foundatio

Genomic Epidemiology of Clinical Brucella melitensis Isolates from Southern Israel

Brucellosis, a zoonosis mainly transmitted by consumption of unpasteurized dairy products, is endemic in Southern Israel, mainly among the Bedouin Arab population. However, the genomic epidemiology of B. melitensis in this region has not yet been elucidated. A cohort of brucellosis cases (n = 118) diagnosed between 2017–2019 was studied using whole-genome sequencing (WGS). Phylogenetic analyses utilized core genome MLST (cgMLST) for all local isolates and core genome SNPs for 347 human-associated B. melitensis genomes, including Israeli and publicly available sequences. Israeli isolates formed two main clusters, presenting a notable diversity, with no clear dominance of a specific strain. On a global scale, the Israeli genomes clustered according to their geographical location, in proximity to genomes originating from the Middle East, and formed the largest cluster in the tree, suggesting relatively high conservation. Our study unveils the genomic epidemiology of B. melitensis in Southern Israel, implicating that rather than a common source, the transmission pattern of brucellosis among Bedouin communities is complex, predominantly local, and household-based. Further, genomic surveillance of B. melitensis is expected to inform future public health and veterinary interventions and clinical care

Estimation of farmers' risk attitude: an econometric approach

An econometric procedure for estimating Arrow-Pratt coefficients of risk aversion is derived. The model of farmers allocating land among different crops, and time between leisure and labor, allows for testing Arrow's hypotheses of decreasing absolute risk aversion and increasing relative risk aversion. The empirical results support these hypotheses

Long-Read Sequencing and Hybrid Assembly for Genomic Analysis of Clinical Brucella melitensis Isolates

Brucella melitensis is a key etiological agent of brucellosis and has been increasingly subject to characterization using sequencing methodologies. This study aimed to investigate and compare short-read, long-read, and hybrid assemblies of B. melitensis. Eighteen B. melitensis isolates from Southern Israel were sequenced using Illumina and the Oxford Nanopore (ONP) MinION, and hybrid assemblies were generated with ONP long reads scaffolded on Illumina short reads. Short reads were assembled with INNUca with SPADes, long reads and hybrid with dragonflye. Abricate with the virulence factor database (VFDB) and in silico PCR (for the genes BetB, BPE275, BSPB, manA, mviN, omp19, perA, PrpA, VceC, and ureI) were used for identifying virulence genes, and a total of 61 virulence genes were identified in short-read, long-read, and hybrid assemblies of all 18 isolates. The phylogenetic analysis using long-read assemblies revealed several inconsistencies in cluster assignment as compared to using hybrid and short-read assemblies. Overall, hybrid assembly provided the most comprehensive data, and stand-alone short-read sequencing provided comparable data to stand-alone long-read sequencing regarding virulence genes. For genomic epidemiology studies, stand-alone ONP sequencing may require further refinement in order to be useful in endemic settings

Pliocene-Pleistocene climate of the northern margin of Saharan-Arabian Desert recorded in speleothems from the Negev Desert, Israel

The Middle-Late Pliocene climate was 2-3°C warmer than today, but with similar levels of atmospheric CO2. This period reflects climate conditions expected in the near future, and is therefore an important target for current data-modeling studies. This study reconstructs the Pliocene-Quaternary evolution of arid conditions on the northern margin of the Saharan-Arabian desert, using radiometrically (U-Pb) dated periods of speleothem deposition from three caves of the Negev Desert, Israel. Speleothem growth started between ~3.75 and ~3Ma, at the end of the first significant tectonic uplift of the western shoulder of the Dead Sea Rift. Major speleothem deposition, indicating wet conditions, occurred during the Pliocene around ~3.1Ma, with subsequent aridity during the last 3Myr, punctuated by short wet episodes - the Pleistocene Negev Humid Periods (NHP). The oldest dated NHP occurred between ~1.7 and ~1.25Ma, and other short humid episodes continued intermittently later. Speleothem δ18O values (-6.9‰ to -11.2‰) show that the humid episodes were associated with periods of low global ice volume and warm temperatures. After correction for rainfall δ18O changes associated with ice volume and temperature effects, the observed relative constancy of speleothem δ18O values from Pliocene to Late Pleistocene indicates rainfall was from a common source, most probably the eastern Mediterranean Sea. The humid Pliocene conditions could be generated by more southerly position of the Mediterranean coast (allowing the access of Mediterranean precipitation to the Negev), as well as by warmer Atlantic Sea Surface Temperatures, that weakened the Azores High Pressure Cell. Maximum amounts of precipitation were 500-600mm/a during the Pliocene and >300mm/a during Pleistocene NHP. The Pliocene and the earliest Pleistocene NHP are associated with formation of lakes in the Negev. Low 87Sr/86Sr ratios of ~0.7078 in the Pliocene speleothems are indicative of low dust supply, low water residence time in the vadose zone and relatively high weathering rates of the cave host rock. Increase of 87Sr/86Sr ratios to 0.7082-0.7083 in the Pleistocene suggest an increased supply of desert dust, high water residence time in the vadose zone and reduced host rock weathering

Speleothem stable isotopes from 670 - 240 ka ago at Cape Limeworks Cave 1, Robertson, South Africa

We present stable oxygen and carbon isotope data and U-Th chronology data from five speleothems from Cape Limeworks Cave 1 near Robertson, Western Cape, South Africa (33.73S°, 19.77E°). The data set covers the time interval between 240 and 670 ka BP with hiatuses at 630-500 ka and 360-310 ka. The data are used to reconstruct climatic and environmental variability in an area known for its extraordinary plant biodiversity. Speleothems were extracted from the cave using a hammer and chisels and stable isotopes were analyzed at the Geological Survey of Israel using methods described in Bar-Matthews et al., (2003, Geochimica et Cosmochimica Acta, 67, 3181–3199. https://doi.org/10.1016/S0016-7037(02)01031-1) and Bar-Matthews et al., (1997, Quaternary Research, 47, 155–168. https://doi.org/10.1006/qres.1997.1883). U-series dating was done at the Geological Survey of Israel following methods described in Grant et al., (2012, Nature, 491(7426), 744–747. https://doi.org/10.1038/nature11593) and at the University of Minnesota following methods described in Edwards et al., (1987, Earth and Planetary Science Letters, 81, 175–192), Shen et al., (2012, Geochimica et Cosmochimica Acta, 99, 71–86. https://doi.org/10.1016/J.GCA.2012.09.018) and Cheng et al., (2013, Earth and Planetary Science Letters, 371–372, 82–91. https://doi.org/10.1016/j.epsl.2013.04.006)