Role of brachytherapy in the treatment of cancers of the anal canal. Long-term follow-up and multivariate analysis of a large monocentric retrospective series.

BACKGROUND AND PURPOSE: There are few data on long-term clinical results and tolerance of brachytherapy in anal canal cancer. We present one of the largest retrospective analyses of anal canal cancers treated with external beam radiotherapy with/without (±) chemotherapy followed by a brachytherapy boost. MATERIALS AND METHODS: We performed a retrospective analysis of clinical results in terms of efficacy and toxicity. The impact of different clinical and therapeutic variables on these outcomes was studied. RESULTS: From May 1992 to December 2009, 209 patients received brachytherapy after external beam radiotherapy ± chemotherapy. Of these patients, 163 were stage II or stage IIIA (UICC 2002) and 58 were N1-3. According to age, ECOG performance status (PS), and comorbidities, patients received either radiotherapy alone (58/209) or radiochemotherapy (151/209). The median follow-up was 72.8 months. The 5- and 10-year local control rates were 78.6 and 73.9 %, respectively. Globally, severe acute and late G3-4 reactions (NCI-CTC scale v. 4.0) occurred in 11.2 and 6.3 % of patients, respectively. Univariate analysis showed the statistical impact of the pelvic treatment volume (p = 0.046) and of the total dose (p = 0.02) on the risk of severe acute and late toxicities, respectively. Only six patients required permanent colostomy because of severe late anorectal toxicities. CONCLUSION: After a long follow-up time, brachytherapy showed an acceptable toxicity profile and high local control rates in patients with anal canal cancer

Clinical trials in children: Equity, quality and relevance

This thesis investigates the equity, quality and relevance of clinical trials in children to inform better evidence-based child healthcare and outcomes worldwide. A comprehensive review of the literature revealed that despite current initiatives to encourage more trials in children, there is still a paucity of safety and efficacy data of many medicines prescribed in this population. An analysis of trials registered in children showed that disease burden was moderately correlated to trials and this scarcity was particularly prevalent in low-and middle-income countries. We explored the contributory factors to this inequity by conducting a systematic review of stakeholders’ views of trials in children in low-and middle-income countries. In the study evaluating the completeness of protocols of trials in children submitted to ethics committees, we found that protocols are generally comprehensive, but many key domains in trial design and conduct are not reported. Key-informant trial stakeholders who were interviewed proposed strategies to improve trials such as addressing the unique needs of children, embedding trials as part of routine clinical care and streamlining regulatory approvals. Increasing international collaboration, establishing sustainable centralised trials infrastructure, and aligning research to child health priorities were proposed to encourage more high-quality trials that address global child healthcare needs

Low-frequency radio emission in the massive galaxy cluster MACS J0717.5 + 3745

International audienceTo investigate the nonthermal emission mechanism and their interaction during cluster mergers, we analyze multiple low-frequency radio data for the X-ray luminous massive galaxy cluster MACS J0717.5 + 3745, located at z = 0.5548. Large-scale structure-formation models in the Universe suggest that galaxy clusters grow via constant accretion of gas and the merger of galaxy groups and smaller clusters. Low-frequency radio observations trace these mergers in the form of relics and halos. The dual frequency observations were performed on MACS J0717.5 + 3745 to investigate the spectral index pattern of the nonthermal emission and its interaction within the intracluster medium (ICM), during merger process. Methods: Continuum observations were carried out using GMRT at 0.235 and 0.61 GHz on MACS J0717.5 + 3745 and archival data from the VLA (0.074 and 1.42 GHz) and WSRT (0.325 GHz) was used to complement the results. Furthermore, to explore the thermal and nonthermal interactions within the ICM and the morphological distribution, Chandra X-ray and HST data were used. Results: A highly complex nonthermal radio emission distribution is seen in the cluster at very low frequencies, with a global spectral index α0.2350.61˜-1.17±0.37. We have detected a giant radio halo within the cluster system with a linear size of 1.58 Mpc and a "Chair-shaped" filament structure between the merging subclusters of linear size 853 kpc at 0.235 GHz. This is the most powerful halo ever observed with P1.4 = 9.88 × 1025 WHz-1 and an equipartition magnetic field estimate of ~6.49 μG. The bright filament structure is well located in the central merging region of subclusters with enhanced temperature, as shown by Chandra and HST data analysis, further indicating the formation of this structure due to shock waves encountered within the ICM during the merger events

Airborne Sunphotometer and Solar Spectral Flux Radiometer Measurements During INTEX/ITCT 2004

During the period 12 July - 8 August 2004, the NASA Ames 14-channel Airborne Tracking Sunphotometer (MTS-14) and Solar Spectral Flux Radiometer (SSFR) were operated aboard a Jetstream 31 (J31) aircraft and acquired measurements during nineteen science flights (approx. 53 flight hours) over the Gulf of Maine in support of the INTEX-NA (INtercontinental chemical Transport Experiment-North America) and ITCT (Intercontinental Transport and Chemical Transformation of anthropogenic pollution) field studies. In this paper, we will present results from analyses of those data sets. AATS-14 measures the direct solar beam transmission at fourteen discrete wavelengths (354-2138 nm), and provides instantaneous measurements of aerosol optical depth (AOD) spectra and water vapor column content, in addition to vertical profiles of aerosol extinction and water vapor density during suitable aircraft ascents and descents. SSFR consists of separate nadir and zenith viewing hemispheric FOV sensors that yield measurements of up- and downwelling solar irradiance at a spectral resolution of approx. 8-12 nm over the wavelength range 300-1700 nm. The objectives of the J31-based measurements during INTEX/ITCT were to provide AOD data for the evaluation of MODIS (MODerate-resolution Imaging Spectroradiometer) and MISR (Multi-angle Imaging Spectro-Radiometer) AOD retrievals, quantify sea surface spectral albedo (which can contribute the largest uncertainty to satellite aerosol retrievals for low aerosol loading), test closure (consistency) among suborbital results, test chemical-transport models using AOD profiles, and assess regional radiative forcing by combining satellite and suborbital results. Specific J31 flight patterns were designed to achieve these objectives, and they included a mixture of vertical profiles (spiral and ramped ascents and descents) and constant altitude horizontal transects at a variety of altitudes. Additional information is included in the original extended abstract

The FFAG R&D and medical application project RACCAM

JACoW web site http://accelconf.web.cern.ch/AccelConf/e06/Pre-Press/WEPCH161.pdf WEPCH161International audienceThe RACCAM project (Recherche en ACCelerateurs et Applications Medicales) has recently obtained fundings, extending over three years (2006-2008), from the French National Research Agency (ANR). RACCAM is a tripartite collaboration, involving (i) the CNRS Laboratory IN2P3/LPSC, (ii) the French magnet industrial SIGMAPHI, and (iii) the nuclear medecine Departement of Grenoble Hospital. The project concerns fixed field alternating gradient accelerator (FFAG) research on the one hand, and on the other hand their application as hadrontherapy and biology research machines. RACCAM's goal is three-fold, (i) participate to the on-going international collaborations in the field of FFAGs and recent concepts of "non-scaling" FFAGs, with frames for instance, the Neutrino Factory (NuFact) and the EMMA project of an electron model of a muon FFAG accelerator, (ii) design, build and experiment a prototype of an FFAG magnet proper to fulfil the requirements of rapid cycling acceleration, (iii) develop the concepts, and show the feasibility, of the application of such FFAG beams to hadrontherapy and to biology research

Mosquito magnet® liberty plus trap baited with octenol confirmed best candidate for Anopheles surveillance and proved promising in predicting risk of malaria transmission in French Guiana

BACKGROUND: In French Guiana, Mosquito Magnet(®) Liberty Plus trap baited with octenol (MMoct) has been proposed for sampling Anopheles darlingi after comparison with CDC light trap and Human landing catch (HLC). However, other available lures were not tested. The current study compared MMoct and MM baited with Lurex™ (MMlur) to HLC, and analysed entomological data from MMoct collection with malaria cases to facilitate malaria surveillance. METHODS: Two independent experiments were conducted during 2012 and 2013 in Saint-Georges town, French Guiana. The first experiment used Latin square design to compare MMoct and MMlur to HLC between 18:30 to 22:30 and 05:00 to 07:00. Parity rate was determined for An. darlingi from each sampling system. In the second experiment, a 24:00 hour collection was done for four consecutive days during the first week of each month and every four days for the rest of the month using MMoct. Portion of the 24 hour collection was dissected for parity rate. All anophelines were screened for Plasmodium infection by PCR. Data for number of malaria cases was analysed for association with density of An. darlingi. RESULTS: In the first experiment, 3,721 anopheline mosquitoes were collected over 21 nights. Of these, 95.7% was identified morphologically to five species and An. darlingi contributed 98.4%, mainly from HLC (75.1%, CI 95% [73.2-77.0]) than MMoct (14.1%, CI 95% [12.6-15.7]) and MMlur (10.8%, CI 95% [9.4-12.2]). Species richness was highest in HLC meanwhile species diversity index was greatest in MMoct. MMoct collected more parous An. darlingi than HLC (p < 0.0001) and MMlur (p = 0.0021). The second experiment amounted to 2035 females, 60.8% belonging to 10 species. Anopheles darlingi constituted 85.0% of the species and had parity rate of 52.3%. Specimens were uninfected with Plasmodium. Density of An. darlingi best correlated with malaria cases observed six weeks later (p = 0.0016; r = 0.4774). CONCLUSION: Though MMoct and MMlur performed well in sampling An. darlingi, MMoct captured more species and, therefore, would be useful for surveillance. Even if it collected mostly parous mosquitoes, MMoct proved useful in collecting entomological data required for predicting malaria emergence. It is a potential replacement for HLC

Heterogeneous Batch Distillation Processes: Real System Optimisation

In this paper, optimisation of batch distillation processes is considered. It deals with real systems with rigorous simulation of the processes through the resolution full MESH differential algebraic equations. Specific software architecture is developed, based on the BatchColumn® simulator and on both SQP and GA numerical algorithms, and is able to optimise sequential batch columns as long as the column transitions are set. The efficiency of the proposed optimisation tool is illustrated by two case studies. The first one concerns heterogeneous batch solvent recovery in a single distillation column and shows that significant economical gains are obtained along with improved process conditions. Case two concerns the optimisation of two sequential homogeneous batch distillation columns and demonstrates the capacity to optimize several sequential dynamic different processes. For such multiobjective complex problems, GA is preferred to SQP that is able to improve specific GA solutions

Magnetic relaxation measurements of exchange biased (Pt/Co) multilayers with perpendicular anisotropy

Magnetic relaxation measurements were carried out by magneto-optical Kerr effect on exchange biased (Pt/Co)5/Pt/FeMn multilayers with perpendicular anisotropy. In these films the coercivity and the exchange bias field vary with Pt spacer thickness, and have a maximum for 0.2 nm. Hysteresis loops do not reveal important differences between the reversal for ascending and descending fields. Relaxation measurements were fitted using Fatuzzo's model, which assumes that reversal occurs by domain nucleation and domain wall propagation. For 2 nm thick Pt spacer (no exchange bias) the reversal is dominated by domain wall propagation starting from a few nucleation centers. For 0.2 nm Pt spacer (maximum exchange bias) the reversal is strongly dominated by nucleation, and no differences between the behaviour of the ascending and descending branches can be observed. For 0.4 nm Pt spacer (weaker exchange bias) the nucleation density becomes less important, and the measurements reveal a much stronger density of nucleation centers in the descending branch.Comment: Europhysical Journal B, in print DOI: 10.1140/epjb/e2005-00053-

Exonuclease VII repairs quinolone-induced damage by resolving DNA gyrase cleavage complexes

The widely used quinolone antibiotics act by trapping prokaryotic type IIA topoisomerases, resulting in irreversible topoisomerase cleavage complexes (TOPcc). Whereas the excision repair pathways of TOPcc in eukaryotes have been extensively studied, it is not known whether equivalent repair pathways for prokaryotic TOPcc exist. By combining genetic, biochemical, and molecular biology approaches, we demonstrate that exonuclease VII (ExoVII) excises quinolone-induced trapped DNA gyrase, an essential prokaryotic type IIA topoisomerase. We show that ExoVII repairs trapped type IIA TOPcc and that ExoVII displays tyrosyl nuclease activity for the tyrosyl-DNA linkage on the 5′-DNA overhangs corresponding to trapped type IIA TOPcc. ExoVII-deficient bacteria fail to remove trapped DNA gyrase, consistent with their hypersensitivity to quinolones. We also identify an ExoVII inhibitor that synergizes with the antimicrobial activity of quinolones, including in quinolone-resistant bacterial strains, further demonstrating the functional importance of ExoVII for the repair of type IIA TOPcc

Vertically resolved aerosol optical properties over the ARM SGP site

We will present an overview of early airborne results obtained aboard the Center for Interdisciplinary Remotely-Piloted Aircraft Studies (CIRP AS) Twin Otter aircraft during the Atmospheric Radiation Measurement (ARM) program aerosol intensive observation period in May 2003