A far UV study of interstellar gas towards HD34078: high excitation H2 and small scale structure - Based on observations performed by the FUSE mission and at the CFHT telescope

To investigate the presence of small scale structure in the spatial distribution of H2 molecules we have undertaken repeated FUSE UV observations of the runaway O9.5V star, HD34078. In this paper we present five spectra obtained between January 2000 and October 2002. These observations reveal an unexpectedly large amount of highly excited H2. Column densities for H2 levels from (v = 0, J = 0) up to (v = 0, J = 11) and for several v = 1 and v = 2 levels are determined. These results are interpreted in the frame of a model involving essentially two components: i) a foreground cloud (unaffected by HD34078) responsible for the H2 (J = 0, 1), CI, CH, CH+ and CO absorptions; ii) a dense layer of gas (n = 10E4 cm-3) close to the O star and strongly illuminated by its UV flux which accounts for the presence of highly excited H2. Our model successfully reproduces the H2 excitation, the CI fine-structure level populations as well as the CH, CH+ and CO column densities. We also examine the time variability of H2 absorption lines tracing each of these two components. From the stability of the J = 0, 1 and 2 damped H2 profiles we infer a 3 sigma upper limit on column density variations Delta(N(H2))/N(H2) of 5% over scales ranging from 5 to 50 AU. This result clearly rules out any pronounced ubiquitous small scale "density" structure of the kind apparently seen in HI. The lines from highly excited gas are also quite stable (equivalent to Delta(N)/N <= 30%) indicating i) that the ambient gas through which HD34078 is moving is relatively uniform and ii) that the gas flow along the shocked layer is not subject to marked instabilitie

The Stellar Composition of the Star Formation Region CMa R1 -- III. A new outburst of the Be star component in Z CMa

We report on a recent event in which, after more than a decade of slowly fading, the visual brightness of the massive young binary Z CMa suddenly started to rise by about 1 magnitude in December 1999, followed by a rapid decline to its previous brightness over the next six months. This behaviour is similar to that exhibited by this system around its eruption in February 1987. A comparison of the intrinsic luminosities of the system with recent evolutionary calculations shows that Z CMa may consist of a 16 M_sun B0 IIIe primary star and a ~ 3 M_sun FUOr secondary with a common age of ~ 3 x 10^5 yr. We also compare new high-resolution spectra obtained in Jan. and Feb. 2000, during the recent rise in brightness, with archive data from 1991 and 1996. The spectra are rich in emission lines, which originate from the envelope of the early B-type primary star. The strength of these emission lines increased strongly with the brightness of Z CMa. We interpret the collected spectral data in terms of an accretion disc with atmosphere around the Herbig B0e component of Z CMa, which has expanded during the outbursts of 1987 and 2000. A high resolution profile of the 6300 A [O I] emission line, obtained by us in March 2002 shows an increase in flux and a prominent blue shoulder to the feature extending to ~ -700 km/s, which was much fainter in the pre-outburst spectra. We propose that this change in profile is a result of a strong change in the collimation of a jet, as a result of the outburst at the start of this century.Comment: 22 pages, 12 figures, accepted for publication in MNRA

Comparison and characterization of α-amylase inducers in Aspergillus nidulans based on nuclear localization of AmyR

AmyR, a fungal transcriptional activator responsible for induction of amylolytic genes in Aspergillus nidulans, localizes to the nucleus in response to the physiological inducer isomaltose. Maltose, kojibiose, and d-glucose were also found to trigger the nuclear localization of GFP-AmyR. Isomaltose- and kojibiose-triggered nuclear localization was not inhibited by the glucosidase inhibitor, castanospermine, while maltose-triggered localization was inhibited. Thus, maltose itself does not appear to be an direct inducer, but its degraded or transglycosylated product does. Non-metabolizable d-glucose analogues were also able to trigger the nuclear localization, implying that these sugars, except maltose, directly function as the inducers of AmyR nuclear entry. The inducing activity of d-glucose was 4 orders-of-magnitude weaker compared with isomaltose. Although d-glucose has the ability to induce α-amylase production, this activity would generally be masked by CreA-dependent carbon catabolite repression. Significant induction of α-amylase by d-glucose was observed in creA-defective A. nidulans

The CRE1 carbon catabolite repressor of the fungus Trichoderma reesei: a master regulator of carbon assimilation

<p>Abstract</p> <p>Background</p> <p>The identification and characterization of the transcriptional regulatory networks governing the physiology and adaptation of microbial cells is a key step in understanding their behaviour. One such wide-domain regulatory circuit, essential to all cells, is carbon catabolite repression (CCR): it allows the cell to prefer some carbon sources, whose assimilation is of high nutritional value, over less profitable ones. In lower multicellular fungi, the C2H2 zinc finger CreA/CRE1 protein has been shown to act as the transcriptional repressor in this process. However, the complete list of its gene targets is not known.</p> <p>Results</p> <p>Here, we deciphered the CRE1 regulatory range in the model cellulose and hemicellulose-degrading fungus <it>Trichoderma reesei </it>(anamorph of <it>Hypocrea jecorina</it>) by profiling transcription in a wild-type and a delta-<it>cre1 </it>mutant strain on glucose at constant growth rates known to repress and de-repress CCR-affected genes. Analysis of genome-wide microarrays reveals 2.8% of transcripts whose expression was regulated in at least one of the four experimental conditions: 47.3% of which were repressed by CRE1, whereas 29.0% were actually induced by CRE1, and 17.2% only affected by the growth rate but CRE1 independent. Among CRE1 repressed transcripts, genes encoding unknown proteins and transport proteins were overrepresented. In addition, we found CRE1-repression of nitrogenous substances uptake, components of chromatin remodeling and the transcriptional mediator complex, as well as developmental processes.</p> <p>Conclusions</p> <p>Our study provides the first global insight into the molecular physiological response of a multicellular fungus to carbon catabolite regulation and identifies several not yet known targets in a growth-controlled environment.</p

Identification of the CRE-1 Cellulolytic Regulon in Neurospora crassa

Background: In filamentous ascomycete fungi, the utilization of alternate carbon sources is influenced by the zinc finger transcription factor CreA/CRE-1, which encodes a carbon catabolite repressor protein homologous to Mig1 from Saccharomyces cerevisiae. In Neurospora crassa, deletion of cre-1 results in increased secretion of amylase and b-galactosidase. Methodology/Principal Findings: Here we show that a strain carrying a deletion of cre-1 has increased cellulolytic activity and increased expression of cellulolytic genes during growth on crystalline cellulose (Avicel). Constitutive expression of cre-1 complements the phenotype of a N. crassa Dcre-1 strain grown on Avicel, and also results in stronger repression of cellulolytic protein secretion and enzyme activity. We determined the CRE-1 regulon by investigating the secretome and transcriptome of a Dcre-1 strain as compared to wild type when grown on Avicel versus minimal medium. Chromatin immunoprecipitation-PCR of putative target genes showed that CRE-1 binds to only some adjacent 59-SYGGRG-39 motifs, consistent with previous findings in other fungi, and suggests that unidentified additional regulatory factors affect CRE-1 binding to promoter regions. Characterization of 30 mutants containing deletions in genes whose expression level increased in a Dcre-1 strain under cellulolytic conditions identified novel genes that affect cellulase activity and protein secretion

Identification of the promoter region involved in the autoregulation of the transcriptional activator ALCR in Aspergillus nidulans.

The ALCR protein is the transcriptional activator of the ethanol utilization pathway in the filamentous fungus Aspergillus nidulans. This activator belongs to a family of fungal proteins having a conserved DNA-binding domain containing six cysteines (C6 class) with some striking features. At variance with other motifs of this class, the binding domain of ALCR is strongly asymmetrical in relation to the central cysteines and moreover was predicted to adopt a helix-turn-helix structure. This domain of ALCR was synthesized in Escherichia coli and purified as a glutathione-S-transferase fusion protein. Our results show that the transcriptional activator ALCR is a DNA-binding protein. The DNA-binding motif contains zinc that is necessary for the specific DNA binding. The ALCR peptide binds upstream of the coding region of alcR to two specific targets with different affinities that are characterized by a conserved 5-nucleotide core, 5'-CCGCA-3' (or its reverse). One site, the lower-affinity binding site, is a direct repeat, and the other, the higher-affinity binding site, is a palindromic sequence with dyad symmetry. Therefore, the ALCR binding protein is able to recognize one DNA sequence in two different configurations. An alcR mutant obtained by deletion of the two specific targets in the cis-acting region of the alcR gene is unable to grow on ethanol and does not express any alcohol dehydrogenase activity. These results demonstrate that the binding sites are in vivo functional targets (UASalc) for the ALCR protein in A. nidulans. They corroborate prior evidence that alcR is autoregulated