119 research outputs found
Artefacts in the estimation of adp analogs as phosphate acceptors in mitochondrial oxidative phosphorylation
Structural and functional characterization of the Mycobacterium tuberculosis uridine monophosphate kinase: insights into the allosteric regulationâ
Nucleoside Monophosphate Kinases (NMPKs) family are key enzymes in nucleotide metabolism. Bacterial UMPKs depart from the main superfamily of NMPKs. Having no eukaryotic counterparts they represent attractive therapeutic targets. They are regulated by GTP and UTP, while showing different mechanisms in Gram(+), Gram(â) and archaeal bacteria. In this work, we have characterized the mycobacterial UMPK (UMPKmt) combining enzymatic and structural investigations with site-directed mutagenesis. UMPKmt exhibits cooperativity toward ATP and an allosteric regulation by GTP and UTP. The crystal structure of the complex of UMPKmt with GTP solved at 2.5âĂ
, was merely identical to the modelled apo-form, in agreement with SAXS experiments. Only a small stretch of residues was affected upon nucleotide binding, pointing out the role of macromolecular dynamics rather than major structural changes in the allosteric regulation of bacterial UMPKs. We further probe allosteric regulation by site-directed mutagenesis. In particular, a key residue involved in the allosteric regulation of this enzyme was identified
Spectrophotometric determination of H2O2-generating oxidases using oxyhemoglobin as oxygen donor and indicator
Sensitive and fast simultaneous photometric determination of oxygen consumption and NAD(P)H formation or disappearance using a continuous flow cuvette
Standardized device for the assay of oxygen consumption adaptable to commercial photometers
SÄrata Monteoru. SÄpÄturile arheologice din Poiana ScoruĹului din 1952 Ĺi 1954 / SÄrata Monteoru. Les fouilles archĂŠologiques de Poiana ScoruĹului (dĂŠp. de BuzÄu). Rapport prĂŠliminaire pour les annĂŠes 1952â1954
Zaharia Eugenia, Bârzu Ligia. SÄrata Monteoru. SÄpÄturile arheologice din Poiana ScoruĹului din 1952 Ĺi 1954 / SÄrata Monteoru. Les fouilles archĂŠologiques de Poiana ScoruĹului (dĂŠp. de BuzÄu). Rapport prĂŠliminaire pour les annĂŠes 1952â1954. In: Materiale Ĺi cercetĂŁri arheologice (Serie nouĂŁ), N°1 2001. 1999. pp. 41-58
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