32 research outputs found
International study to evaluate PCR methods for detection of Trypanosoma cruzi DNA in blood samples from Chagas disease patients
A century after its discovery, Chagas disease, caused by the parasite Trypanosoma cruzi, still represents a major neglected tropical threat. Accurate diagnostics tools as well as surrogate markers of parasitological response to treatment are research priorities in the field. The polymerase chain reaction (PCR) has been proposed as a sensitive laboratory tool for detection of T. cruzi infection and monitoring of parasitological treatment outcome. However, high variation in accuracy and lack of international quality controls has precluded reliable applications in the clinical practice and comparisons of data among cohorts and geographical regions. In an effort towards harmonization of PCR strategies, 26 expert laboratories from 16 countries evaluated their current PCR procedures against sets of control samples, composed by serial dilutions of T.cruzi DNA from culture stocks belonging to different lineages, human blood spiked with parasite cells and blood samples from Chagas disease patients. A high variability in sensitivities and specificities was found among the 48 reported PCR tests. Out of them, four tests with best performance were selected and further evaluated. This study represents a crucial first step towards device of a standardized operative procedure for T. cruzi PCR.Fil: Schijman, Alejandro G. Instituto de Investigaciones en IngenierĂa GenĂ©tica y BiologĂa Molecular (INGEBI-CONICET). Laboratorio de BiologĂa Molecular de la Enfermedad de Chagas (LabMECh); Argentina.Fil: Bisio, Margarita. Instituto de Investigaciones en IngenierĂa GenĂ©tica y BiologĂa Molecular (INGEBI-CONICET). Laboratorio de BiologĂa Molecular de la Enfermedad de Chagas (LabMECh); Argentina.Fil: Orellana, Liliana. Universidad de Buenos Aires. Instituto de CĂĄlculo; Argentina.Fil: Sued, Mariela. Universidad de Buenos Aires. Instituto de CĂĄlculo; Argentina.Fil: Duffy, TomĂĄs. Instituto de Investigaciones en IngenierĂa GenĂ©tica y BiologĂa Molecular (INGEBI-CONICET). Laboratorio de BiologĂa Molecular de la Enfermedad de Chagas (LabMECh); Argentina.Fil: Mejia Jaramillo, Ana M. Universidad de Antioquia. Grupo Chagas; Colombia.Fil: Cura, Carolina. Instituto de Investigaciones en IngenierĂa GenĂ©tica y BiologĂa Molecular (INGEBI-CONICET). Laboratorio de BiologĂa Molecular de la Enfermedad de Chagas (LabMECh); Argentina.Fil: Auter, Frederic. French Blood Services; Francia.Fil: Veron, Vincent. Universidad de ParasitologĂa. Laboratorio Hospitalario; Guayana Francesa.Fil: Qvarnstrom, Yvonne. Centers for Disease Control. Department of Parasitic Diseases; Estados Unidos.Fil: Deborggraeve, Stijn. Institute of Tropical Medicine; BĂ©lgica.Fil: Hijar, Gisely. Instituto Nacional de Salud; PerĂș.Fil: Zulantay, InĂ©s. Facultad de Medicina; Chile.Fil: Lucero, RaĂșl Horacio. Universidad Nacional del Nordeste; Argentina.Fil: VelĂĄzquez, Elsa. ANLIS Dr.C.G.MalbrĂĄn. Instituto Nacional de ParasitologĂa Dr. Mario Fatala Chaben; Argentina.Fil: Tellez, Tatiana. Universidad Mayor de San Simon. Centro Universitario de Medicina Tropical; Bolivia.Fil: Sanchez Leon, Zunilda. Universidad Nacional de AsunciĂłn. Instituto de Investigaciones en Ciencias de la Salud; Paraguay.Fil: GalvĂŁo, Lucia. Faculdade de FarmĂĄcia; Brasil.Fil: Nolder, Debbie. Hospital for Tropical Diseases. London School of Tropical Medicine and Hygiene Department of Clinical Parasitology; Reino Unido.Fil: Monje Rumi, MarĂa. Universidad Nacional de Salta. Laboratorio de PatologĂa Experimental; Argentina.Fil: Levi, JosĂ© E. Hospital Sirio LibanĂȘs. Blood Bank; Brasil.Fil: Ramirez, Juan D. Universidad de los Andes. Centro de Investigaciones en MicrobiologĂa y ParasitologĂa Tropical; Colombia.Fil: Zorrilla, Pilar. Instituto Pasteur; Uruguay.Fil: Flores, MarĂa. Instituto de Salud Carlos III. Centro de Mahahonda; España.Fil: Jercic, Maria I. Instituto Nacional De Salud. SecciĂłn ParasitologĂa; Chile.Fil: Crisante, Gladys. Universidad de los Andes. Centro de Investigaciones ParasitolĂłgicas J.F. Torrealba; Venezuela.Fil: Añez, NĂ©stor. Universidad de los Andes. Centro de Investigaciones ParasitolĂłgicas J.F. Torrealba; Venezuela.Fil: De Castro, Ana M. Universidade Federal de GoiĂĄs. Instituto de Patologia Tropical e SaĂșde PĂșblica (IPTSP); Brasil.Fil: Gonzalez, Clara I. Universidad Industrial de Santander. Grupo de InmunologĂa y EpidemiologĂa Molecular (GIEM); Colombia.Fil: Acosta Viana, Karla. Universidad AutĂłnoma de YucatĂĄn. Departamento de Biomedicina de Enfermedades Infecciosas y Parasitarias Laboratorio de BiologĂa Celular; MĂ©xico.Fil: Yachelini, Pedro. Universidad CatĂłlica de Santiago del Estero. Instituto de Biomedicina; Argentina.Fil: Torrico, Faustino. Universidad Mayor de San Simon. Centro Universitario de Medicina Tropical; Bolivia.Fil: Robello, Carlos. Instituto Pasteur; Uruguay.Fil: Diosque, Patricio. Universidad Nacional de Salta. Laboratorio de PatologĂa Experimental; Argentina.Fil: Triana Chavez, Omar. Universidad de Antioquia. Grupo Chagas; Colombia.Fil: Aznar, Christine. Universidad de ParasitologĂa. Laboratorio Hospitalario; Guayana Francesa.Fil: Russomando, Graciela. Universidad Nacional de AsunciĂłn. Instituto de Investigaciones en Ciencias de la Salud; Paraguay.Fil: BĂŒscher, Philippe. Institute of Tropical Medicine; BĂ©lgica.Fil: Assal, Azzedine. French Blood Services; Francia.Fil: Guhl, Felipe. Universidad de los Andes. Centro de Investigaciones en MicrobiologĂa y ParasitologĂa Tropical; Colombia.Fil: Sosa Estani, Sergio. ANLIS Dr.C.G.MalbrĂĄn. Centro Nacional de DiagnĂłstico e InvestigaciĂłn en Endemo-Epidemias; Argentina.Fil: DaSilva, Alexandre. Centers for Disease Control. Department of Parasitic Diseases; Estados Unidos.Fil: Britto, Constança. Instituto Oswaldo Cruz/FIOCRUZ. LaboratĂłrio de Biologia Molecular e Doenças EndĂȘmicas; Brasil.Fil: Luquetti, Alejandro. LaboratĂłrio de Pesquisa de Doença de Chagas; Brasil.Fil: Ladzins, Janis. World Health Organization (WHO). Special Programme for Research and Training in Tropical Diseases (TDR); Suiza
International Study to Evaluate PCR Methods for Detection of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients
A century after its discovery, Chagas disease, caused by the parasite Trypanosoma cruzi, still represents a major neglected tropical threat. Accurate diagnostics tools as well as surrogate markers of parasitological response to treatment are research priorities in the field. The polymerase chain reaction (PCR) has been proposed as a sensitive laboratory tool for detection of T. cruzi infection and monitoring of parasitological treatment outcome. However, high variation in accuracy and lack of international quality controls has precluded reliable applications in the clinical practice and comparisons of data among cohorts and geographical regions. In an effort towards harmonization of PCR strategies, 26 expert laboratories from 16 countries evaluated their current PCR procedures against sets of control samples, composed by serial dilutions of T.cruzi DNA from culture stocks belonging to different lineages, human blood spiked with parasite cells and blood samples from Chagas disease patients. A high variability in sensitivities and specificities was found among the 48 reported PCR tests. Out of them, four tests with best performance were selected and further evaluated. This study represents a crucial first step towards device of a standardized operative procedure for T. cruzi PCR
Le virus
Le virus West Nile (WNV) ou virus du Nil occidental fait parler de lui depuis les annĂ©es 1990 par les Ă©pidĂ©mies quâil a provoquĂ©es autour du bassin mĂ©diterranĂ©en et en Europe centrale. Depuis son apparition en 1999 en AmĂ©rique du Nord, le WNV est devenu la principale cause dâencĂ©phalites arbovirales aux Ătats-Unis. Il sâest rĂ©pandu au cours des dix derniĂšres annĂ©es sur lâensemble du continent nord-amĂ©ricain. La sĂ©vĂ©ritĂ© des symptĂŽmes observĂ©s et la transmission du virus lors de transfusions sanguines et de transplantations dâorganes ont nĂ©cessitĂ© la mise en place de tests de dĂ©pistage, de rĂ©seaux de surveillance et dâĂ©tudes pour mieux comprendre lâĂ©cologie, lâĂ©pidĂ©miologie et la biologie de cet agent pathogĂšne. En Europe, les Ă©pidĂ©mies ont Ă©tĂ© plus limitĂ©es, mais leur rĂ©currence dans certaines rĂ©gions est prĂ©occupante. Une collaboration internationale sâest organisĂ©e pour Ă©viter la transmission de ce flavivirus Ă©mergent et pour comprendre sa biologie afin de dĂ©velopper de futurs traitements
Microwave absorbent material based on glass waste and carbon fibers
International audienc
Synthesis and characterization of foam glass composites for electromagnetic absorption application
International audienc
Elaboration and characterization of foams glass composites for electromagnetic waves insulation application
International audienc
Waste tire-based glass foam composites for microwave absorption application
International audienc
Glass foam composites based on used tires for microwave absorbing application
International audienc
Viral load and clinical manifestations of hepatitis E virus genotype 3 infections
International audienc